Endrews Delbaje

Universidade de São Paulo

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Marli Fátima Fiore

Universidade de São Paulo

Gustavo H. Goldman

Universidade de São Paulo

David P. Fewer

University of Helsinki

Patrícia Alves de Castro

Universidade de São Paulo

Camila Figueiredo Pinzan

Universidade de Ribeirão Preto

Matti Wahlsten

University of Helsinki

Jouni Jokela

University of Helsinki

Thaila Fernanda dos Reis

Universidade de São Paulo

Antonis Rokas

Vanderbilt University

Ernani Pinto

Universidade de São Paulo

Cooperative Institutions

Universidade de São Paulo

University of Helsinki

Universidade de Ribeirão Preto

Methodist University of Piracicaba

Universidade Estadual de Campinas (UNICAMP)

Hospital Universitário da Universidade de São Paulo

Vanderbilt University

Biocenter Finland

Instituto de Ciências Farmacêuticas

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)

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Bacterial Communities Along Environmental Gradients in Tropical Soda Lakes

Research Square (Research Square) (2022)

Thierry A. Pellegrinetti Simone Raposo Cotta Hugo Sarmento Juliana Sampaio da Costa Endrews Delbaje

Abstract Soda lakes environment is known to be variable and can have distinct differences according to geographical location. In this study, we investigated the effect of different environmental conditions of six adjacent soda lakes on bacterial communities and their functioning using a metagenomic approach combined with flow cytometry and chemical analyses. Ordination analysis using flow cytometry and water chemistry data from two sampling periods (wet and dry) clustered soda lakes in three different profiles: eutrophic turbid (ET), oligotrophic turbid (OT), and clear vegetated oligotrophic (CVO). Analysis of bacterial community composition and functioning corroborated this ordination; the exception was one ET lake, that was similar to one OT lake during the wet season, indicating drastic shifts between seasons. Microbial abundance and diversity increased during the dry period, along with a considerable number of limnological variables, all indicative of a strong effect of the precipitation-evaporation balance in these systems. Cyanobacteria were linked to high electric conductivity, pH, and nutrient availability, whereas Actinobacteria, Alphaproteobacteria, and Betaproteobacteria were correlated with landscape morphology variability (surface water, surface perimeter, and lake volume) and less stressed lake conditions. Stress response metabolism was overrepresented in ET and OT lakes and underrepresented in CVO lakes. Altogether, this study illustrated the sensitivity of tropical soda lakes to climate change, as slight changes in hydrological regimes might produce drastic shifts in community diversity.

Dry season

Betaproteobacteria

10.21203/rs.3.rs-1196209/v1

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Direct Evidence of Microbial Sunscreen Production by Scum‐Forming Cyanobacteria in the Baltic Sea

Environmental Microbiology Reports (2025)

Inkeri Vuori Greta Gaiani Sıla Arsın Endrews Delbaje Julia Järn

ABSTRACT Mycosporine‐like amino acids are water‐soluble secondary metabolites that protect photosynthetic microorganisms from ultraviolet radiation. Here, we present direct evidence for the production of these compounds in surface scums of cyanobacteria along the Baltic Sea coast. We collected 59 environmental samples from the southern coast of Finland during the summers of 2021 and 2022 and analysed them using high‐resolution liquid chromatography‐mass spectrometry. Our results revealed the presence of microbial sunscreens in nearly all surface scum samples. Mycosporine‐like amino acids are synthesised through the coordinated action of four biosynthetic enzymes encoded in a compact biosynthetic pathway. Bioinformatics analysis of the mysB biosynthetic gene from a surface scum indicated that the cyanobacteria responsible for production belonged to the Anabaena / Dolichospermum / Aphanizomenon species complex. We mapped the distribution of biosynthetic enzymes onto a phylogenomic tree, utilising 120 bacterial single‐copy conserved genes from 101 draft or complete genomes within the species complex. This analysis showed that 48% of identified species possess the ability to produce these compounds, with biosynthetic pathways being most common in Dolichospermum and Aphanizomenon strains. We detected the production of porphyra‐334 and shinorine, two widely reported family members, in Dolichospermum strains isolated from the Gulf of Finland's surface layer. The estimated content of porphyra‐334 in Dolichospermum sp. UHCC 0684 was 7.4 mg per gram dry weight. Our results suggest that bloom‐forming cyanobacteria could be a potential source of these compounds for cosmetic and biotechnological applications and may play a significant role in cyanobacterial bloom formation.

Baltic sea

10.1111/1758-2229.70056

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A phylogenetic approach to explore the Aspergillus fumigatus conidial surface-associated proteome and its role in pathogenesis

Research Square (Research Square) (2023)

Gustavo H. Goldman Clara Valero Camila Figueiredo Pinzan Patrícia de Castro Norman van Rhijn

, an important pulmonary fungal pathogen causing several diseases collectively called aspergillosis, relies on asexual spores (conidia) for initiating host infection. Here, we used a phylogenomic approach to compare proteins in the conidial surface of

Proteome

Conidiation

10.21203/rs.3.rs-3306535/v1

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Espécie do hospedeiro, micro-habitat e época do ano modulam a estrutura das comunidades de micro-organismos diazotróficos na floresta Amazônica

Endrews Delbaje

10.11606/d.11.2019.tde-10102018-134610

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Aspergillus fumigatus conidial surface-associated proteome reveals factors for fungal evasion and host immunity modulation

Nature Microbiology (2024)

Camila Figueiredo Pinzan Clara Valero Patrícia Alves de Castro Jefferson Luiz da Silva Kayleigh Earle

Proteome

Fungal protein

Pathogenic fungus

10.1038/s41564-024-01782-y

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Identification of a homoarginine biosynthetic gene from a microcystin biosynthetic pathway in Fischerella sp. PCC 9339

Toxicon (2024)

Xiaodan Ouyang Matti Wahlsten Maija Pollari Endrews Delbaje Jouni Jokela

Microcystins (MCs) are a family of chemically diverse toxins produced by numerous distantly related cyanobacteria. They are potent inhibitors of eukaryotic protein phosphatases 1 and 2A and are responsible for the toxicosis and death of wild and domestic animals around the world. Microcystins are synthesized on a large enzyme complex comprised of peptide synthetases, polyketide synthases, and additional modifying enzymes. Bioinformatic analysis identified the presence of an additional uncharacterized enzyme in the microcystin (mcy) biosynthetic gene cluster in Fischerella sp. PCC 9339, which we named McyK, that lacked a clearly defined role in the biosynthesis of microcystin. Further bioinformatic analysis suggested that McyK belongs to the inosamine-phosphate amidinotransferase family and could be involved in synthesizing homo amino acids. Quadrupole time-of-flight tandem mass spectrometry (Q-TOFMS/MS) analysis confirmed that Fischerella sp. PCC 9339 produces MC-Leucine2-Homoarginine4(MC-LHar) and [Aspartic acid3]MC-Leucine2-Homoarginine4 ([Asp3]MC-LHar) as the dominant chemical variants. We hypothesized that the McyK enzyme might be involved in the production of microcystin variants containing homoarginine (Har) in the strain. Heterologous expression of a codon-optimized mcyK gene in Escherichia coli confirmed that McyK is responsible for the synthesis of L-Har. These results confirm the production of MC-LHar, a novel microcystin chemical variant [Asp3]MC-LHar, and a new microcystin biosynthetic enzyme involved in supply of the rare homo-amino acid Har to the microcystin biosynthetic pathway in Fischerella sp. PCC 9339. This study provides new insights into the logic underpinning the biosynthesis of microcystin chemical variants and broadens our knowledge of structural diversity of microcystins.

Nonribosomal peptide

Heterologous expression

Polyketide synthase

Gene cluster

10.1016/j.toxicon.2024.107733

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Effect of ultraviolet radiation on the metabolomic profiles of potentially toxic cyanobacteria

FEMS Microbiology Ecology (2020)

Fernanda Rios Jacinavicius Vanessa Geraldes Camila M. Crnkovic Endrews Delbaje Marli Fátima Fiore

ABSTRACT Interactions between climate change and ultraviolet radiation (UVR) have a substantial impact on aquatic ecosystems, especially on photosynthetic organisms. To counteract the damaging effects of UVR, cyanobacteria developed adaptive strategies such as the biosynthesis of secondary metabolites. This study aimed to evaluate the effects of UVR on the metabolomic profiles of potentially toxic cyanobacteria. Twelve strains were irradiated with ultraviolet A and ultraviolet B radiation and parabolic aluminized reflector lamps for 3 days, followed by liquid chromatography–tandem mass spectometry (LC-MS/MS) analysis to assess changes in metabolomic profiles. Matrices were used to generate principal component analysis biplots, and molecular networks were obtained using the Global Natural Products platform. Most strains showed significant changes in their metabolomic profiles after UVR exposure. On average, 7% of MS features were shown to be exclusive to metabolomic profiles before UVR exposure, while 9% were unique to metabolomic profiles after UVR exposure. The identified compounds included aeruginosins, spumigins, cyanopeptolins, microginins, namalides, pseudospumigins, anabaenopeptins, mycosporine-like amino acids, nodularins and microcystins. Data showed that cyanobacteria display broad metabolic plasticity upon UVR exposure, including the synthesis and differential expression of a variety of secondary metabolites. This could result in a competitive advantage, supporting cyanobacterial blooms under various UVR light exposures.

10.1093/femsec/fiaa243

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Citations (19)

The Influence of Aspergillus fumigatus Fatty Acid Oxygenases PpoA and PpoC on Caspofungin Susceptibility

Journal of Fungi (2024)

Endrews Delbaje Patrícia Alves de Castro Dante G. Calise Mengyao Niu Maria Augusta Crivelente Horta

can cause invasive pulmonary aspergillosis (IPA). Fungicidal azoles and fungistatic caspofungin (CAS) are the first- and second-line therapies, respectively, used to treat IPA. Treatment of

10.3390/jof10110749

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A Plastic Biosynthetic Pathway for the Production of Structurally Distinct Microbial Sunscreens

ACS Chemical Biology (2023)

Sıla Arsın Endrews Delbaje Jouni Jokela Matti Wahlsten Zoe May Farrar

Mycosporine-like amino acids (MAAs) are small, colorless, and water-soluble secondary metabolites. They have high molar extinction coefficients and a unique UV radiation absorption mechanism that make them effective sunscreens. Here we report the discovery of two structurally distinct MAAs from the lichen symbiont strain Nostoc sp. UHCC 0926. We identified these MAAs as aplysiapalythine E (C23H38N2O15) and tricore B (C34H53N4O15) using a combination of high-resolution liquid chromatography–mass spectrometry (HR-LCMS) analysis and nuclear magnetic resonance (NMR) spectroscopy. We obtained a 8.3 Mb complete genome sequence of Nostoc sp. UHCC 0926 to gain insights into the genetic basis for the biosynthesis of these two structural distinct MAAs. We identified MAA biosynthetic genes encoded in three separate locations of the genome. The organization of biosynthetic enzymes in Nostoc sp. UHCC 0926 necessitates a branched biosynthetic pathway to produce two structurally distinct MAAs. We detected the presence of such discontiguous MAA biosynthetic gene clusters in 12% of the publicly available complete cyanobacterial genomes. Bioinformatic analysis of public MAA biosynthetic gene clusters suggests that they are subject to rapid evolutionary processes resulting in highly plastic biosynthetic pathways that are responsible for the chemical diversity in this family of microbial sunscreens.

Nostoc

Metabolic pathway

Secondary metabolism

10.1021/acschembio.3c00112

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Evolutionary origin and population diversity of a cryptic hybrid pathogen

Nature Communications (2024)

Jacob L. Steenwyk S E Knowles Rafael Wesley Bastos Charu Balamurugan David C. Rinker

10.1038/s41467-024-52639-1

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Citations (4)