Susana Fuentes

National Institute for Public Health and the Environment

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Willem M. de Vos

University of Helsinki

Sudarshan A. Shetty

Wageningen University & Research

Mercedes Monteoliva‐Sánchez

Universidad de Granada

Hauke Smidt

Wageningen University & Research

Erwin G. Zoetendal

Wageningen University & Research

Eelco Franz

National Institute for Public Health and the Environment

Debbie van Baarle

University Medical Center Groningen

Jolanda Kool

National Institute for Public Health and the Environment

A. Ramos‐Cormenzana

Universidad de Granada

Debby Bogaert

Centre for Inflammation Research

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National Institute for Public Health and the Environment

University of Amsterdam

Amsterdam University Medical Centers

Academic Medical Center

Wageningen University & Research

University Medical Center Utrecht

University Medical Center

Utrecht University

Istituti di Ricovero e Cura a Carattere Scientifico

Radboud University Nijmegen

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Consenso intersocietario para el tratamiento de la obesidad en adultos en Argentina

Revista de la Sociedad Argentina de Diabetes (2023)

Marianela Aguirre Ackermann María Victoria Salinas Marina Torresani Ana María Cappelletti Lilia Cafaro

La obesidad es una enfermedad crónica que genera altos costos en salud, cuyo tratamiento, en ocasiones, no se aborda o se lo hace de forma inadecuada, y muchas veces no se basa en la evidencia disponible. Es por ello que, a partir de una iniciativa de la Sociedad Argentina de Nutrición, diferentes sociedades científicas se reunieron con el objetivo de construir un documento actualizado, basado en una metodología científica, que aporte al mejor tratamiento de las personas que viven con obesidad en Argentina. Este es el primer Consenso Intersocietario para el tratamiento de la Obesidad en adultos en Argentina a partir del cual se obtuvieron recomendaciones para la práctica clínica basadas en la evidencia científica. Es una necesidad primordial contar con herramientas, adecuadas a la realidad local y consensuadas por expertos de distintas disciplinas.

10.47196/diab.v57i3.718

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Chromohalobacter nigrandesensis sp. nov., a moderately halophilic, Gram-negative bacterium isolated from Lake Tebenquiche on the Atacama Saltern, Chile

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY (2006)

Bernardo Prado Catherine Lizama Jaime Aguilera A. Ramos‐Cormenzana Susana Fuentes

A total of 52 strains of moderately halophilic bacteria isolated from hypersaline sediment of Lake Tebenquiche on the Atacama Saltern, Chile, were subjected to a taxonomic study. The morphological, physiological, biochemical and nutritional characteristics of the strains matched those described for the genus Chromohalobacter . Cells were Gram-negative, non-spore-forming, rod-shaped and motile. A black pigmentation was produced. One strain, designated LTS-4N T , grew optimally at 32 °C. Growth occurred in media containing 0·5–25 % (w/v) total salts; the optimum was 7·5 % (w/v) total salts. The pH range for growth was 5–10. The G+C content of the DNA of strain LTS-4N T was 59·8 mol%. Analysis of 16S rRNA gene sequence similarity revealed that strain LTS-4N T was closely related to Chromohalobacter species; however, DNA–DNA hybridization of representative strain LTS-4N T failed to associate the strain with any species of the genus Chromohalobacter with validly published names. Therefore, the name Chromohalobacter nigrandesensis sp. nov. is proposed. The type strain is LTS-4N T (=CECT 5315 T =DSM 14323 T ).

Strain (injury)

10.1099/ijs.0.63983-0

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Citations (27)

A strain of Lactobacillus plantarum a¡ects segmented¢lamentous bacteria in the intestineof immunosuppressedmice

Susana Fuentes Markus Egert María Jiménez‐Valera Mercedes Monteoliva‐Sánchez Alfonso Ruiz‐Bravo

A strain of Lactobacillus plantarum aiects segmented¢lamentous bacteria in the intestineof immunosuppressedmice Susana Fuentes, Markus Egert, Maria Jimenez-Valera, Mercedes Monteoliva-Sanchez, Alfonso Ruiz-Bravo & Hauke Smidt Department of Microbiology, School of Pharmacy, University of Granada, Granada, Spain; Laboratory of Microbiology, Agrotechnology and Food Sciences Group, Wageningen University, Wageningen, The Netherlands; and Top Institute Food and Nutrition, Nieuwe Kanaal, Wageningen, The Netherlands

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The relationship between faecal-associated and mucosal-associated microbiota in irritable bowel syndrome patients and healthy subjects

Alimentary Pharmacology & Therapeutics (2015)

Igancio Rangel Johanna Sundin Susana Fuentes Dirk Repsilber Willem M. de Vos

The faecal-associated microbiota is commonly seen as a surrogate of the mucosal-associated microbiota. However, previous studies indicate that they are different. Furthermore, analyses of the mucosal microbiota are commonly done after standard bowel cleansing, affecting the microbial composition.To compare the mucosal-associated microbiota, obtained from unprepared colon, with faecal-associated microbiota in healthy subjects and irritable bowel syndrome (IBS) patients.Faecal and mucosal biopsies were obtained from 33 IBS patients and 16 healthy controls. Of IBS patients, 49% belonged to the diarrhoea-predominant subgroup and 80% suffered from IBS symptoms during at least 5 years. Biopsies were collected from unprepared sigmoid colon and faecal samples a day before colonoscopy. Microbiota analyses were performed with a phylogenetic microarray and redundancy discriminant analysis.The composition of the mucosal- and the faecal-associated microbiota in unprepared sigmoid colon differs significantly (P = 0.002). Clinical characteristics of IBS did not correlate with this difference. Bacteroidetes dominate the mucosal-associated microbiota. Firmicutes, Actinobacteria and Proteobacteria dominate the faecal-associated microbiota. Healthy subjects had a significantly higher (P < 0.005) abundance (1.9%) of the bacterial group uncultured Clostridiales I in the mucosal-associated microbiota than IBS patients (0.3%). Bacterial diversity was higher in faecal- compared with mucosal-associated microbiota in IBS patients (P < 0.005). No differences were found in healthy subjects.Differences in the mucosal-associated microbiota between healthy individuals and IBS patients are minimal (one bacterial group) compared to differences in the faecal microbiota of both groups (53 bacterial groups). Microbial aberrations characterising IBS are more pronounced in the faeces than in the mucosa.

Dysbiosis

10.1111/apt.13399

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Associations between Pro- and Anti-Inflammatory Gastro-Intestinal Microbiota, Diet, and Cognitive Functioning in Dutch Healthy Older Adults: The NU-AGE Study

Nutrients (2020)

Annick P. M. van Soest Gerben D. A. Hermes Agnes A. M. Berendsen Ondine van de Rest Erwin G. Zoetendal

Dietary modulation of the gastro-intestinal microbiota is a potential target in improving healthy ageing and age-related functional outcomes, including cognitive decline. We explored the association between diet, gastro-intestinal microbiota and cognition in Dutch healthy older adults of the 'New dietary strategies addressing the specific needs of the elderly population for healthy aging in Europe' (NU-AGE) study. The microbiota profile of 452 fecal samples from 226 subjects was determined using a 16S ribosomal RNA gene-targeted microarray. Dietary intake was assessed by 7-day food records. Cognitive functioning was measured with an extensive cognitive test battery. We observed a dietary and microbial pro- to anti-inflammatory gradient associated with diets richer in animal- or plant-based foods. Fresh fruits, nuts, seeds and peanuts, red and processed meat and grain products were most strongly associated to microbiota composition. Plant-rich diets containing fresh fruits, nuts, seeds and peanuts were positively correlated with alpha-diversity, various taxa from the Bacteroidetes phylum and anti-inflammatory species, including those related to Faecalibacterium prausnitzii and Eubacterium rectale and E. biforme. Animal product-rich diets associated with pro-inflammatory species, including those related to Ruminococcus gnavus and Collinsella spp.. Cognition was neither associated with microbiota composition nor alpha-diversity. In conclusion, diets richer in animal- and plant-based foods were related to a pro- and anti-inflammatory microbial profile, while cognition was associated with neither.

Faecalibacterium prausnitzii

Ruminococcus

Cognitive Decline

10.3390/nu12113471

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Correlation between Protection against Sepsis by Probiotic Therapy and Stimulation of a Novel Bacterial Phylotype

Applied and Environmental Microbiology (2011)

Jacoline Gerritsen Harro M. Timmerman Susana Fuentes L. Paul van Minnen Henk Panneman

Prophylactic probiotic therapy has shown beneficial effects in an experimental rat model for acute pancreatitis on the health status of the animals. Mechanisms by which probiotic therapy interferes with severity of acute pancreatitis and associated sepsis, however, are poorly understood. The aims of this study were to identify the probiotic-induced changes in the gut microbiota and to correlate these changes to disease outcome. Duodenum and ileum samples were obtained from healthy and diseased rats subjected to pancreatitis for 7 days and prophylactically treated with either a multispecies probiotic mixture or a placebo. Intestinal microbiota was characterized by terminal-restriction fragment length polymorphism (T-RFLP) analyses of PCR-amplified 16S rRNA gene fragments. These analyses showed that during acute pancreatitis the host-specific ileal microbiota was replaced by an "acute pancreatitis-associated microbiota." This replacement was not reversed by administration of the probiotic mixture. An increase, however, was observed in the relative abundance of a novel bacterial phylotype most closely related to Clostridium lituseburense and referred to as commensal rat ileum bacterium (CRIB). Specific primers targeting the CRIB 16S rRNA gene sequence were developed to detect this phylotype by quantitative PCR. An ileal abundance of CRIB 16S rRNA genes of more than 7.5% of the total bacterial 16S rRNA gene pool was correlated with reduced duodenal bacterial overgrowth, reduced bacterial translocation to remote organs, improved pancreas pathology, and reduced proinflammatory cytokine levels in plasma. Our current findings and future studies involving this uncharacterized bacterial phylotype will contribute to unraveling one of the potential mechanisms of probiotic therapy.

Phylotype

Bacterial Genetics

10.1128/aem.05428-11

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Human intestinal microbiota composition is associated with local and systemic inflammation in obesity

Obesity (2013)

Froukje J. Verdam Susana Fuentes Charlotte de Jonge Erwin G. Zoetendal Runi Erbil

Intestinal microbiota have been suggested to contribute to the development of obesity, but the mechanism remains elusive. The relationship between microbiota composition, intestinal permeability, and inflammation in nonobese and obese subjects was investigated.Fecal microbiota composition of 28 subjects (BMI 18.6-60.3 kg m(-2) ) was analyzed by a phylogenetic profiling microarray. Fecal calprotectin and plasma C-reactive protein levels were determined to evaluate intestinal and systemic inflammation. Furthermore, HbA1c , and plasma levels of transaminases and lipids were analyzed. Gastroduodenal, small intestinal, and colonic permeability were assessed by a multisaccharide test.Based on microbiota composition, the study population segregated into two clusters with predominantly obese (15/19) or exclusively nonobese (9/9) subjects. Whereas intestinal permeability did not differ between clusters, the obese cluster showed reduced bacterial diversity, a decreased Bacteroidetes/Firmicutes ratio, and an increased abundance of potential proinflammatory Proteobacteria. Interestingly, fecal calprotectin was only detectable in subjects within the obese microbiota cluster (n = 8/19, P = 0.02). Plasma C-reactive protein was also increased in these subjects (P = 0.0005), and correlated with the Bacteroidetes/Firmicutes ratio (rs = -0.41, P = 0.03).Intestinal microbiota alterations in obese subjects are associated with local and systemic inflammation, suggesting that the obesity-related microbiota composition has a proinflammatory effect.

Proinflammatory cytokine

Intestinal Permeability

10.1002/oby.20466

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CONSENSO INTERSOCIETARIO PARA EL TRATAMIENTO DE LA OBESIDAD EN ADULTOS EN ARGENTINA

Actualización en Nutrición (2023)

Marianela Aguirre Ackermann María Victoria Salinas Marina Torresani Ana María Cappelletti Lilia Cafaro

10.48061/san.2022.24.3.132

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An Explorative Biomarker Study for Vaccine Responsiveness after a Primary Meningococcal Vaccination in Middle-Aged Adults

Frontiers in Immunology (2018)

Marieke van der Heiden Guy A. M. Berbers Susana Fuentes Menno C. van Zelm Annemieke M. H. Boots

Prevention of infectious diseases in the elderly is essential to establish healthy aging. Yet, immunological aging impairs successful vaccination of the elderly. Predictive biomarkers for vaccine responsiveness in middle-aged adults may help to identify responders and non-responders before reaching old age. Therefore, we aimed to determine biomarkers associated with low and high responsiveness toward a primary vaccination in middle-aged adults, for which a tetravalent meningococcal vaccine was used as a model.Middle-aged adults (50-65 years of age, N = 100), receiving a tetravalent meningococcal vaccination, were divided into low and high responders using the functional antibody titers at 28 days postvaccination. A total of 48 parameters, including absolute numbers of immune cells and serum levels of cytokines and biochemical markers, were determined prevaccination in all participants. Heat maps and multivariate redundancy analysis (RDA) were used to reveal immune phenotype characteristics and associations of the low and high responders.Several significant differences in prevaccination immune markers were observed between the low and high vaccine responders. Moreover, RDA analysis revealed a significant association between the prevaccination immune phenotype and vaccine responsiveness. In particular, our analysis pointed at high numbers of CD4 T cells, especially naïve CD4 and regulatory T subsets, to be associated with low vaccine responsiveness. In addition, low responders showed lower prevaccination IL-1Ra levels than high responders.This explorative biomarker study shows associations between the prevaccination immune phenotype and vaccine responsiveness after a primary meningococcal vaccination in middle-aged adults. Consequently, these results provide a basis for predictive biomarker discovery for vaccine responsiveness that will require validation in larger cohort studies.

Immunosenescence

Healthy aging

Healthy ageing

Meningococcal vaccine

10.3389/fimmu.2017.01962

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Succession of early microbial consortia in the developing infant gut microbiota unveiled by meta-omics approaches

Federica Del Chierico Pamela Vernocchi Stefano Levi Mortera Luca Laghi Lucia Vannini

Omics

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