Rastislav Monošík

University of Copenhagen

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Miroslav Streďanský

Biorealis (Slovakia)

EŠ

Ernest Šturdı́k

Slovak University of Technology in Bratislava

Lars Ove Dragsted

University of Copenhagen

John Nielsen

University of Copenhagen

Ján Tkáč

Institute of Chemistry of the Slovak Academy of Sciences

Lúcio Angnes

Universidade de São Paulo

Lesli H. Larsen

University of Copenhagen

Gabriel Greif

Slovak University of Technology in Bratislava

Malene W. Poulsen

University of Copenhagen

Monika Bąk

University of Gdańsk

Cooperative Institutions

University of Copenhagen

Slovak University of Technology in Bratislava

Slovak Academy of Sciences

Institute of Chemistry of the Slovak Academy of Sciences

Frederiksberg Hospital

Nutrition Sciences (Belgium)

Copenhagen University Hospital

Technical University of Denmark

Universidade de São Paulo

Novo Nordisk Foundation

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Biosensors - classification, characterization and new trends

Acta Chimica Slovaca (2012)

Rastislav Monošík Miroslav Streďanský Ernest Šturdı́k

Biosensors - classification, characterization and new trends Biosensors represent promising analytical tools applicable in areas such as clinical diagnosis, food industry, environment monitoring and in other fields, where rapid and reliable analyses are needed. Some biosensors were successfully implemented in the commercial sphere, but majority needs to be improved in order to overcome some imperfections. This review covers the basic types, principles, constructions and use of biosensors as well as new trends used for their fabrication.

Characterization

10.2478/v10188-012-0017-z

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Citations (249)

Comparison of biosensors based on gold and nanocomposite electrodes for monitoring of malic acid in wine

Open Chemistry (2011)

Rastislav Monošík Miroslav Streďanský Gabriel Greif Ernest Šturdı́k

Abstract Amperometric biosensors based on a gold planar electrode and on two types of nanocomposite electrodes consisting of multi-walled carbon nanotubes for the determination of L-malic acid designed for wine-makers were developed. The biosensors designed for wine-makers were constructed by immobilization of L-malate dehydrogenase and diaphorase within chitosan layers on the surface of the electrodes. The coenzyme NAD+ and the electrochemical mediator ferricyanide were present in the measuring solution. The current resulting from re-oxidation of produced ferrocyanide was measured at a working potential of +300 mV against an Ag/AgCl reference electrode. The biosensor based on a gold electrode showed linearity over the range 10–520 µM with a detection limit of 5.41 µM. Calibration curves for biosensors utilizing nanocomposites were obtained both with the linear range of 10 to 610 µM. The detection limits were 1.57 and 1.77 µM, respectively. The biosensors showed satisfactory operational stability (no loss of sensitivity after 30 consecutive measurements) and storage stability (90% of the initial sensitivity after one year of storage at room temperature). The results obtained from measurements of wine samples were in a good correlation with the standard HPLC method. Satisfactory biosensor sensitivity, specificity and stability allowed their successful commercialization.

Amperometry

Ferrocyanide

Ferricyanide

Prussian blue

Malic acid

10.2478/s11532-011-0118-3

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Citations (22)

A Biosensor Utilizing l-Glutamate Dehydrogenase and Diaphorase Immobilized on Nanocomposite Electrode for Determination of l-Glutamate in Food Samples

Food Analytical Methods (2012)

Rastislav Monošík Miroslav Streďanský Ernest Šturdı́k

Amperometry

Diaphorase

Ferricyanide

10.1007/s12161-012-9468-5

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Citations (27)

Poly(lactic acid)-based Nanocomposite for Construction of Efficient Bilirubin Oxidase-Based Biocathodes and Stable Biofuel Cells

International Journal of Electrochemical Science (2014)

Jaroslav Filip Rastislav Monošík Ján Tkáč

A modification of electrode surfaces with dispersions of carbon black and consequent enzyme adsorption has been recognized as an efficient technique for construction of biofuel cells (BFCs). To achieve this, typically fluorinated polymers with a negative environmental impact are used as dispersing agents. In this work, these were replaced by an abundant, biodegradable and cost-effective biopolymer poly(lactic acid) (PLA) for construction of novel BFCs in a green and sustainable way. The electron transfer rate of bilirubin oxidase (BOD) within a biocathode, often limiting overall performance of BFCs, was greatly enhanced to the highest value of (300 ± 2) s-1 reported so far by introduction of a PLA matrix. The BFC was completed by a combination of a BOD biocathode with a fructose dehydrogenase bioanode, offering a power density of 57 μW cm-2 at 400 mV. A scanning electron microscopy of scaled up BFC device, prepared by deposition of PLA containing nanocomposites on carbon paper, revealed a highly porous structure, a feature important to deal with a limited diffusion of a biofuel and oxygen within a 3D matrix. The fructose-oxygen BFC offered an excellent operational stability comparable to the best ones reported for similar BFCs with 50% of an initial current/power observed after 10 days of storage.

Bilirubin oxidase

Glucose oxidase

Carbon fibers

Biopolymer

10.1016/s1452-3981(23)07942-7

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A simple paper-strip colorimetric method utilizing dehydrogenase enzymes for analysis of food components

Analytical Methods (2015)

Rastislav Monošík Vagner Bezerra dos Santos Lúcio Angnes

A simple chromatographic paper based colorimetric assay for the analysis of selected food compounds.

Colorimetry

10.1039/c5ay01556a

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Citations (33)

Short-term effects of dietary advanced glycation end products in rats

British Journal Of Nutrition (2015)

Malene W. Poulsen Jeanette Marker Andersen Rikke V. Hedegaard Andreas Nygaard Madsen Britta N. Krath

Abstract Dietary advanced glycation end products (AGE) formed during heating of food have gained interest as potential nutritional toxins with adverse effects on inflammation and glucose metabolism. In the present study, we investigated the short-term effects of high and low molecular weight (HMW and LMW) dietary AGE on insulin sensitivity, expression of the receptor for AGE ( RAGE ), the AGE receptor 1 ( AGER1 ) and TNF-α , F2-isoprostaglandins, body composition and food intake. For 2 weeks, thirty-six Sprague–Dawley rats were fed a diet containing 20 % milk powder with different proportions of this being given as heated milk powder (0, 40 or 100 %), either native (HMW) or hydrolysed (LMW). Gene expression of RAGE and AGER1 in whole blood increased in the group receiving a high AGE LMW diet, which also had the highest urinary excretion of the AGE, methylglyoxal-derived hydroimidazolone 1 (MG-H1). Urinary excretion of N ε -carboxymethyl-lysine increased with increasing proportion of heat-treated milk powder in the HMW and LMW diets but was unrelated to gene expression. There was no difference in insulin sensitivity, F2-isoprostaglandins, food intake, water intake, body weight or body composition between the groups. In conclusion, RAGE and AGER1 expression can be influenced by a high AGE diet after only 2 weeks in proportion to MG-H1 excretion. No other short-term effects were observed.

RAGE

10.1017/s0007114515004833

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Citations (29)

Application of Enzyme Biosensors in Analysis of Food and Beverages

Food Analytical Methods (2011)

Rastislav Monošík Miroslav Streďanský Ján Tkáč Ernest Šturdı́k

10.1007/s12161-011-9222-4

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Citations (102)

Monitoring of PQQ-Dependent Glucose Dehydrogenase Substrate Specificity for Its Potential Use in Biocatalysis and Bioanalysis

Applied Biochemistry and Biotechnology (2013)

Miroslav Streďanský Rastislav Monošík Vladimı́r Mastihuba Ernest Šturdı́k

Bioanalysis

Bioproduction

Biocatalysis

10.1007/s12010-013-0419-4

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Citations (13)

Application of Electrochemical Biosensors in Clinical Diagnosis

Journal of Clinical Laboratory Analysis (2012)

Rastislav Monošík Miroslav Streďanský Ernest Šturdı́k

Analyses in the clinical area need quick and reliable analytical methods and devices. For this purpose, biosensors can be a suitable option, whereas they are constructed to be simple for use, specific for the target analyte, capable of continuous monitoring and giving quick results, potentially low‐costing and portable. In this article, we describe electrochemical biosensors developed for clinical diagnosis, namely for glucose, lactate, cholesterol, urea, creatinine, DNA, antigens, antibodies, and cancer markers assays. Chosen biosensors showed desirable sensitivity, selectivity, and potential for application on real samples. They are often designed to avoid interference with undesired components present in the monitored systems.

Clinical Diagnosis

10.1002/jcla.20500

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Citations (99)

Multienzymatic amperometric biosensor based on gold and nanocomposite planar electrodes for glycerol determination in wine

Analytical Biochemistry (2011)

Rastislav Monošík Dana Ukropcová Miroslav Streďanský Ernest Šturdı́k

Glycerol kinase

Amperometry

10.1016/j.ab.2011.10.020

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Citations (37)