La producción de pigmento fue estudiada en 80 cepas de S. aureus, 135 de S. epidermidis y 17 cepas de estafilococos inclasificables, aisladas todas ellas de muestras biológicas humanas.
La producción de pigmento en el medio de Chapman-Stone y en el medio de agar leche evidenció un 99 % de concordancias. Se comprobó que el medio de Chapman-Stone era relativamente superior para la producción de pigmento en las capas de S. aureus.
Las cepas multirresistentes, y las cepas con resistencia individual a un antibiótico, no presentaron una alteración cualitativa de la pigmentación.
La producción de pigmento dio un 93 % de concordancias con la producción de coagulasa. y fue la característica más correlacionada con este enzima. En el 96 % de las cepas la pigmentación estaba de acuerdo con la especie taxonómica a que pertenecía la cepa. Se discute la utilidad de la pigmentación como criterio clínico inicial de patogenicidad y como característica esencial del S. aureus, y la no utilidad de la pigmentación como criterio de clasificación taxonómica.
Purpose. The effects of embryonic development on lipid composition in the retina were studied in 7, 11, 15, and 18-day-old chick embryos and newly hatched chicks.Methods. The proportions of phospholipids, free and esterified cholesterol, diacylglycerides, and free fatty acids were determined using the Iatroscan TLC/FID procedure. Gas chromatog-raphy and mass spectrometry were used to determine the fatty acid composition.Results. The major phospholipid species were phosphatidyl-ethanolamine, phosphatidylcholine, phosphatidylserine, phos-phatidylinositol, lysophosphatidylcholine, and sphingomyelin. Concentrations of the analyzed components have been related to the chronology of concrete stages of retinal development. The fatty acid composition of the total lipids, (n-6):(n-3) and saturated:unsaturated fatty acid ratios, and other parameters are reported. The proportions of total saturated and total monoun-saturated fatty acids decreased very little from day 7 to hatching, whereas total polyunsaturated fatty acids nearly doubled over the same period. The increase in C18:2(n-6) from day 11 onwards was not followed by a similar increase in C20:4(n-6), hence the C20:4 to C18:2 ratio decreased with age.Conclusions. The cholesterol: phospholipid ratio decreased from day 7 to day 15 and increased from day 15 to hatching. High proportions of esterified cholesterol, very probably originating in the retinal pigment epithelium, were also recorded. Total saturated and monounsaturated fatty acids decreased, while polyunsaturated fatty acids increased during the period of initial retinal growth.
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