Beom S. Lee

Korea Atomic Energy Research Institute

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Gook-H. Chung

Jeonbuk National University

Mark C. Barnes

James Cook University

Yeong Deuk Jo

Chungnam National University

Si-Yong Kang

Korea Atomic Energy Research Institute

Hong-Il Choi

Korea Atomic Energy Research Institute

Doh-Y. Kim

Seoul National University

Joon‐Woo Ahn

Korea Atomic Energy Research Institute

Nong‐Moon Hwang

Seoul National University

Kwang Min Koo

Korea Atomic Energy Research Institute

Sera Jung

World Institute of Kimchi

Cooperative Institutions

Korea Atomic Energy Research Institute

Seoul National University

Chungnam National University

Jeonbuk National University

Korea Research Institute of Bioscience and Biotechnology

Chonnam National University

James Cook University

University of South Australia

Susquehanna University

Indian Institute of Technology Kharagpur

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The Mechanism of Starch Over-Accumulation in Chlamydomonas reinhardtii High-Starch Mutants Identified by Comparative Transcriptome Analysis

Frontiers in Microbiology (2017)

Kwang Min Koo Sera Jung Beom S. Lee Jin‐Baek Kim Yeong Deuk Jo

The focus of this study was the mechanism of starch accumulation in Chlamydomonas reinhardtii high-starch mutants. Three C. reinhardtii mutants showing high-starch content were generated using gamma irradiation. When grown under nitrogen-deficient conditions, these mutants had more than twice as much starch than a wild-type control. The mechanism of starch over-accumulation in these mutants was studied with comparative transcriptome analysis. In all mutants, induction of phosphoglucomutase 1 (PGM1) expression was detected; PGM1 catalyzes the inter-conversion of glucose 1-phosphate and glucose 6-phosphate in both starch biosynthetic and glycolytic pathway. Interestingly, transcript levels of phosphoglucose isomerase 1 (PGI1), fructose 1,6-bisphosphate aldolase 1 and 2 (FBA1 and FBA2) were down-regulated in all mutants; PGI1, FBA1, and FBA2 act on downstream of glucose 6-phosphate conversion in glycolytic pathway. Therefore, down-regulations of PGI1, FBA1, and FBA2 may lead to accumulation of upstream metabolites, notably glucose 6-phosphate, resulting in induction of PGM1 expression through feed-forward regulation and that PGM1 overexpression caused starch over-accumulation in mutants. These results suggest that PGI1, FBA1, FBA2, and PGM1 correlate with each other in terms of coordinated transcriptional regulation and play central roles for starch over-accumulation in C. reinhardtii.

Chlamydomonas reinhardtii

Glucose-6-phosphate isomerase

Phosphoglucomutase

10.3389/fmicb.2017.00858

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Spontaneous generation of charged clusters of a few nanometers during thermal evaporation of copper

Journal of Crystal Growth (2002)

Beom S. Lee Mark C. Barnes Doh-Y. Kim Nong‐Moon Hwang

Nanometre

Deposition

10.1016/s0022-0248(01)01747-x

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Citations (14)