U Gothe

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R. Lauterbach

R Stracke

Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)

Leo Wollweber

Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)

G Gothe

E. Anders

Edward Via College of Osteopathic Medicine

L Wollweber

Fritsch Rs

Medizinische Hochschule Hannover

Cooperative Institutions

Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)

Friedrich Schiller University Jena

Institute of Molecular Biology

Institute of Molecular Biotechnology

Institute of Molecular Biology and Biotechnology

Edward Via College of Osteopathic Medicine

Landesforschungsanstalt für Landwirtschaft und Fischerei

Fischer (Germany)

Landesforst Mecklenburg‑Vorpommern

Medizinische Hochschule Hannover

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[Effect of preparation procedures on the calculation of average cell volumes from stereological data].

PubMed (1980)

Leo Wollweber Fritsch Rs R Stracke U Gothe

The shrinkage of peritoneal cell during the electron microscopical preparation was investigated by means of a special device. Independently of the procedure of fixation after critical point drying the cells exhibit only 54 to 59% of the original diameter (residual volume about 18%). The reduction of cell diameter after Epon embedding is about 25%, corresponding to a residual volume of 40%.

Stereology

Shrinkage

Residual volume

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A simple immunotest for monitoring the egg maturation in rainbow trout (Oncorhynchus mykiss).

PubMed (1990)

U Gothe L Wollweber G Gothe E. Anders R. Lauterbach

On the principle of the single-radial-immunodiffusion a method has been developed to estimate serum vitellogenin quantitatively in maturing rainbow trouts. Serum vitellogenin (VG) content correlates with the egg diameters. Thus it is possible both to establish the beginning of the egg maturation and to monitor it in a simple way.

Vitellogenin

Radial immunodiffusion

Rainbow

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