Omaira Parra

University of Zulia

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Roger Ramírez

Virginia Tech

Glen Barboza

University of Zulia

Gibson Fernández

Hospital Universitario del Henares

Zulayne Valera

University of Zulia

Cruz María Árraga de Alvarado

University of Zulia

Geovanny Finol

University of Zulia

Cruz Arraga-Alvarado

University of Zulia

ÁM

Álvaro Martínez‐Moreno

University of Córdoba

María Palmar

University of Zulia

Robert Valeris

University of Zulia

Cooperative Institutions

University of Zulia

North Carolina State University

Inserm

Centre National de la Recherche Scientifique

Université de Montpellier

Universitat Autònoma de Barcelona

Universidad Nacional Autónoma de México

Universidad Nacional de Colombia

Virginia Tech

Instituto Nacional de Investigaciones Agricolas

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Expresión y distribución del receptor de complemento gC1qR en los espermatozoides bovinos.

Revista Científica (2008)

Robert Valeris Patricia Villamediana Armando Quintero-Moreno Omaira Parra Jorge Sandoval

The complement receptor gC1qR/p33, which recognizes the globular heads of C1q, is a multicompartmental and multifunctional protein and has been shown to play a role in reproduction in humans. The objective of this research was to determine the gC1qR distribution and expression on plasma membrane in bovine sperm before and after heparin treatment. Thus, capacitation of sperm derived from frozen-thawed bovine semen was induced through heparin incubation and its effectiveness was assessed with a CTC stain. Subsequently, indirect immunofluorescence assays were conducted with mAb (60.11, anti-gC1qR) to assess gC1qR distribution and expression. Data analysis demonstrated that gC1qR is expressed on the plasma membrane of bovine sperm. gC1qR showed a capacitation-related redistribution, migrating to the acrosome region: 175 sperm in heparin-incubated aliquots vs. 109 in control (P<0.05, n=300) showed fluorescence over the acrosome region. This distribution is similar to that reported in humans. Similarly, either gC1qR expression or its accessibility to antibodies increased after capacitation. This also correlates with what has been observed in humans. These results suggest that gC1qR could participate in primary sperm-oocyte interaction in bovines.

Capacitation

Acrosome reaction

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Isolation and identification of microorganisms present in 53 dogs suffering otitis externa

Revista Científica (2006)

Gibson Fernández Glen Barboza Amri Villalobos Omaira Parra Geovanny Finol

Otitis externa is a multi-factorial etiology disease commonly affecting canines. It represents a complex pathology associated to infections caused by bacteria and yeasts, many times not responding to treatments. The goal of this study was to isolate and identify the pathogenic and non-pathogenic micro-organisms existent in otitis externa patients. This research was performed on 53 canine patients which were attended at the Outpatient Consult Service of the University Veterinary Policlinic at Zulia State University (UVP), and which showed clinical signs such as: auditory canal erythema, increased secretion, pain, continuous head shaking and pruritus, purulent secretions and pruritus being the most frequent. Samples were recollected from auditory canal horizontal portion exudates. Different bacteria groups were isolated in culture media, the most frequent were Pseudomona aeruginosa (22.22%), Proteus mirabilis (13.89%), Staphylococcus aureus (12.50%), Staphylococcus epidermidis (8.33%), Escherichia coli (5.56%) and Staphylococcus coagulase-negative (5.56%). In the control group, Bacillus spp. (16.67%), Staphylococcus epidermidis (16.67%) and Staphylococcus coagulase-negative (16.67%) were the most frequently isolated. Cytological examination showed Malassezia pachydermatis to be present in 69.8% of the cases studied. The most affected breeds were Poodles (30.19%), Mongrels (26.42%), Cocker Spaniels (16.98%) and German Shepherd Dogs (9.49%); likewise, a higher incidence of otitis was evidenced in dogs between 2 and 5 months of age (43.40%).

Coagulase

Malassezia

Staphylococcus intermedius

Staphylococcus saprophyticus

Microbiological culture

Etiology

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IMMUNOREACTIVE PROTEINS OF Trypanosoma vivax

Roger Ramírez Zulayne Valera Omaira Parra Everts Chacín Lucinda Tavares-Marques

Bovine trypanosomosis, caused by Trypanosoma vivax has a significant negative impact on livestock. This research was performed with the aim of determining the immunoreactive proteins present in T. vivax . Thus, five sheep were experimentally infected with T. vivax TvZC1 isolate. Animal number 1 was used as the source of the trypanosomes and to prepare the soluble extract of parasites. Sheep numbers 2 to 5 were monitored for eight weeks and sera was obtained every two weeks for immunodetection. Parasites obtained from animal 1 were analyzed for T. vivax proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot (WB). The WB analysis showed three immunodominant proteins with a molecular mass of 42, 64 and 72 kDa, approximately. The 64 kDa protein was recognized by every animal during the complete infection period. The 72 kDa protein only was detected by animals 2, 3 and 5 during the infection course, whereas in animal 4 it was only detected during the 6th and 8th weeks post infection. Moreover, the 42 kDa polypeptide was slightly immunorecognized by animals 2, 3 and 4 during the complete infection period, but in animal 5 only it was identified during the 2th week post infection. It is assumed that the 42 kDa protein is the VSG of T. vivax , which resulted in a low antigenic capacity, contrary to the protein of 64 kDa which showed a high antigenic capacity and cross-reactivity with Trypanosoma evansi.

Trypanosoma vivax

Trypanosoma evansi

Molecular mass

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Respuesta inmunitaria de ovinos (Ovis aries) frente a dos aislados venezolanos de Trypanosoma vivax

Roger Ramírez Francisco Angulo–Cubillán Marcelo Gil Omaira Parra Lucinda Tavares-Marques

La tripanosomosis bovina causada por Trypanosoma vivax tiene un gran impacto economico en la industria ganadera de los paises tropicales. Debido a la escasa informacion existente sobre la respuesta inmunitaria inducida por este parasito en rumiantes, se planifico la presente investigacion con la finalidad de evaluar y comparar la respuesta inmunitaria de ovinos infectados experimentalmente con dos aislados de T. vivax. Ambos aislados fueron obtenidos de diferentes zonas geograficas de Venezuela (TvLIEM176 proveniente del estado Trujillo y TvMT1 del estado Monagas). Tres ovinos fueron inoculados con cada aislado y tres sirvieron como control para un total de nueve animales. Cada tres dias (d), durante un periodo de 60 d se tomaron muestras de suero para realizar la prueba de ELISAi (para determinar la presencia de anticuerpos anti- Trypanosoma spp. ), y sangre para evaluar la parasitemia y realizar un contaje total y diferencial de leucocitos. Los animales del grupo inoculado con el aislado TvMT1 mostraron mayores niveles de anticuerpos antitripanosoma que los animales del grupo TvLIEM176, mientras que la parasitemia se comporto de manera inversa, ya que el aislado TvLIEM176 produjo mayores niveles de parasitemia que TvMT1. Ademas, el aislado TvLIEM176 origino linfopenia durante los d 12 al 36 postinfeccion, mientras que el aislado TvMT1 no. Por lo tanto, se demostro que la respuesta inmunitaria humoral de los dos aislados de T. vivax en ovinos fue diferente, la cual puede deberse a una inmunosupresion causada por el aislado TvLIEM176 al inducir linfopenia.

Trypanosoma vivax

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[Human ehrlichiosis: report of the 1st case in Venezuela].

PubMed (1996)

Cruz Arraga-Alvarado M Montero-Ojeda Alberto Bernardoni Burt Anderson Omaira Parra

Human ehrlichiosis is a zoonotic disease, caused by a rickettsia that infects leukocytes. It was described for the first time in the United States of America in 1986. More than 300 cases have been reported in that country. One case has been reported in Portugal, two in France and one more in a tourist coming from Mali (Africa). In Venezuela, a tropical country, where ehrlichiosis is endemic in dogs and horses, the first case of human ehrlichiosis is reported in a seventeen month old girl. She initially had symptoms compatible with a viral illness. Then she developed a rash, acute respiratory failure, hepato-splenomegaly, neurologic abnormalities, renal failure and hematologic alterations including pancytopenia and disseminated intravascular coagulation (DIC). Different diagnoses were given before it was concluded that it was a case of ehrlichiosis. She was treated with tetracycline and very soon recovered. The initial diagnosis was made using buffy coat blood smears stained with Diff Quick Stain. Indirect Immunofluorescence (IFA) test was used to detect antibodies against Ehrlichia chaffeensis (1:126) but not to other rickettsias. Also the presence of platelets with ehrlichia bodies similar to E. platys from dogs, it was demonstrated, in peripheral blood from the child. The presence of these bodies in human platelets has not been previously reported. In this case, it could not be proved that the disease was transmitted by a tick bite. The presence of ehrlichia bodies in platelets from the girl's peripheral blood is also described.

Ehrlichiosis

Ehrlichia chaffeensis

Buffy coat

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Aislamiento e identificación de microorganismos presentes en 53 perros enfermos de otitis externa

Revista Científica (2006)

Gibson Fernández Glen Barboza Amri Villalobos Omaira Parra Geovanny Finol

Resumen en: Otitis externa is a multi-factorial etiology disease commonly affecting canines. It represents a complex pathology associated to infections caused by b...

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Efecto de la infección experimental con trypanosoma vivax sobre parámetros hematológicos en ovinos

Revista Científica (2005)

Zulayne Valera Omaira Parra Manuel Alvarado Glen Barboza Freddys Escalona

The purpose of this research was to know the effect of experimental infection with a stock of Trypanosoma vivax from Zulia state, Venezuela, on haematologi...

Trypanosoma vivax

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ISOLATION AND IDENTIFICATION OF MICROORGANISMS PRESENT IN 53 DOGS SUFFERING OTITIS EXTERNA Aislamiento e identificación de microorganismos presentes en 53 perros enfermos de otitis externa

Gibson Fernández Glen Barboza Amri Villalobos Omaira Parra Geovanny Finol

Coagulase

Staphylococcus intermedius

Malassezia

Microbiological culture

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Ehrlichiosis equina en el Estado Zulia, Venezuela. Reporte de 232 casos

Cruz María Árraga de Alvarado Geovanny Finol Omaira Parra Miriam Riquelme Américo Savedra

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Prevalencia de Anaplasma marginale en bovinos del sector La Piñata, municipio La Cañada de Urdaneta, estado Zulia, Venezuela

Revista Científica (2003)

Dubraska Díaz Zulayne Valera Erleem de Andrade Omaira Parra Freddys Escalona

Se realizo un estudio con la finalidad de determinar la prevalencia de Anaplasma marginale en bovinos del sector La Pinata, municipio La Canada de Urdaneta, estado Zulia, Venezuela. Para ello fueron seleccionadas 12 fincas, con una poblacion de 6.894 bovinos, evaluandose 174 muestras por medio de la tecnica de Inmunofluorescencia Indirecta (IFI) y la observacion de frotis de capa blanca. A traves de la tecnica de IFI, se obtuvo una prevalencia de 95,4% (166 animales positivos) para Anaplasma marginale; mientras que mediante la observacion de frotis de capa blanca fue de 56,9% (99 animales positivos). No se encontraron diferencias significativas con relacion a la presencia de Anaplasma marginale y el sexo o la edad de los animales. Todas las fincas incluidas en la investigacion mostraron prevalencias de Anaplasma marginale iguales o mayores a 75%, indicando que la zona estudiada presenta una condicion de estabilidad enzootica para este hemoparasito. La tecnica de IFI resulto ser mas efectiva con relacion a la observacion de frotis de capa blanca en la deteccion de animales infectados con Anaplasma marginale.

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