Eduardo F. Abrantes

Hospital Sírio-Libanês

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Luiz F. L. Reis

Hospital Sírio-Libanês

Dirce Maria Carraro

AC Camargo Hospital

Helena Brentani

Universidade de São Paulo

Maria Mitzi Brentani

Instituto do Câncer do Estado de São Paulo

Elisa Napolitano Ferreira

Fleury S.A. (Brazil)

Gustavo de Campos Molina

AC Camargo Hospital

Bedrich L. Ekhardt

The University of Texas MD Anderson Cancer Center

Emmanuel Dias‐Neto

AC Camargo Hospital

Diana Noronha Nunes

AC Camargo Hospital

Wadih Arap

Rutgers, The State University of New Jersey

Cooperative Institutions

Universidade de São Paulo

Hospital Sírio-Libanês

Hospital São Paulo

AC Camargo Hospital

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Fundação Oswaldo Cruz

Universidade Federal de São Paulo

Insper

The University of Texas MD Anderson Cancer Center

Universidade Federal de Minas Gerais

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Correction: Poly (A)⁺Transcriptome Assessment of ERBB2-Induced Alterations in Breast Cell Lines

PLoS ONE (2011)

Dirce Maria Carraro Elisa Napolitano Ferreira Gustavo de Campos Molina Renato Puga Eduardo F. Abrantes

10.1371/annotation/b8888ae6-e402-43af-a425-6fd5e58deef6

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Citations (1)

Benefits in radical mastectomy protocol: a randomized trial evaluating the use of regional anesthesia

Scientific Reports (2018)

Márcio Matsumoto Eva M. Flores Pedro Paulo Kimachi Flavia Venetucci Gouveia Mayra Akemi Kuroki

Abstract Surgery is the first-line treatment for early, localized, or operable breast cancer. Regional anesthesia during mastectomy may offer the prevention of postoperative pain. One potential protocol is the combination of serratus anterior plane block (SAM block) with pectoral nerve block I (PECS I), but the results and potential benefits are limited. Our study compared general anesthesia with or without SAM block + PECS I during radical mastectomy with axillary node dissection and breast reconstruction using evaluations of pain, opioid consumption, side effects and serum levels of interleukin (IL)-1beta, IL-6 and IL-10. This is a prospective, randomized controlled trial. Fifty patients were randomized to general anesthesia only or general anesthesia associated with SAM block + PECS I (25 per group). The association of SAM block + PECS I with general anesthesia reduced intraoperative fentanyl consumption, morphine use and visual analog pain scale scores in the post-anesthetic care unit (PACU) and at 24 h after surgery. In addition, the anesthetic protocol decreased side effects and sedation 24 h after surgery compared to patients who underwent general anesthesia only. IL-6 levels increased after the surgery compared to baseline levels in both groups, and no differences in IL-10 and IL-1 beta levels were observed. Our protocol improved the outcomes of mastectomy, which highlight the importance of improving mastectomy protocols and focusing on the benefits of regional anesthesia.

Modified Radical Mastectomy

10.1038/s41598-018-26273-z

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Citations (57)

Poly (A)+ Transcriptome Assessment of ERBB2-Induced Alterations in Breast Cell Lines

PLoS ONE (2011)

Dirce Maria Carraro Elisa Napolitano Ferreira Gustavo de Campos Molina Renato Puga Eduardo F. Abrantes

We report the first quantitative and qualitative analysis of the poly (A)+ transcriptome of two human mammary cell lines, differentially expressing (human epidermal growth factor receptor) an oncogene over-expressed in approximately 25% of human breast tumors. Full-length cDNA populations from the two cell lines were digested enzymatically, individually tagged according to a customized method for library construction, and simultaneously sequenced by the use of the Titanium 454-Roche-platform. Comprehensive bioinformatics analysis followed by experimental validation confirmed novel genes, splicing variants, single nucleotide polymorphisms, and gene fusions indicated by RNA-seq data from both samples. Moreover, comparative analysis showed enrichment in alternative events, especially in the exon usage category, in ERBB2 over-expressing cells, data indicating regulation of alternative splicing mediated by the oncogene. Alterations in expression levels of genes, such as LOX, ATP5L, GALNT3, and MME revealed by large-scale sequencing were confirmed between cell lines as well as in tumor specimens with different ERBB2 backgrounds. This approach was shown to be suitable for structural, quantitative, and qualitative assessment of complex transcriptomes and revealed new events mediated by ERBB2 overexpression, in addition to potential molecular targets for breast cancer that are driven by this oncogene.

10.1371/journal.pone.0021022

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Identification and characterization of a novel mouse gene encoding a Ras-associated guanine nucleotide exchange factor: expression in macrophages and myocarditis elicited byTrypanosoma cruziparasites

Journal of Leukocyte Biology (2002)

Ludmila Rodrigues Pinto Ferreira Eduardo F. Abrantes Cibele Velloso‐Rodrigues Bráulia Costa Caetano Gustavo Cerqueira

Abstract The ability of Trypanosoma cruzi to activate macrophages is, at least in part, attributed to the glycosylphosphatidylinositol-anchored mucin-like glycoproteins (GPI-mucins) expressed in the surface of the trypomastigote stage of the parasite. The differential display reverse transcriptase-polymerase chain reaction and the reverse Northern blot were used to study modulation of gene expression in murine macrophages exposed to GPI-mucins and in cardiac tissues from mice infected with T. cruzi. Among several cDNAs that were more abundant in lanes corresponding to macrophages stimulated with GPI-mucins as compared with resting cells, we confirmed the differential expression of A1, interleukin-18, and GPIγ4. Some of these genes were also shown to have enhanced expression in the cardiac tissue (DAP-12, A1, and GPIγ4) from infected animals. The expression of GPIγ4 was also enhanced in human monocytes stimulated with GPI-mucins or bacterial lipopolysaccharides. The complete sequence of the GPIγ4 transcript and its gene including the 5′ upstream region was defined. GPIγ4 was encoded by a novel, single copy gene present in mouse as well as human genomes and showed conserved homology to different members of the guanine nucleotide exchange factor family.

Northern blot

Differential display

Chagas Disease

10.1189/jlb.72.6.1215

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Su1980 Diabetes and Dislipidemia Increase Significantly the Risk of Gastric Intestinal Metaplasia: A Prospective Study

Gastroenterology (2015)

Tomás Navarro-Rodríguez Ricardo C. Barbuti Jaime Natan Eisig Ricardo Dib Eduardo F. Abrantes

Warburg Effect

AXIN2

RPTOR

Survivin

10.1016/s0016-5085(15)31909-0

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Application of the differential display RT-PCR strategy for the identification of inflammation-related mouse genes

Brazilian Journal of Medical and Biological Research (1999)

Aristóbolo M. Silva Eliane Pires Eduardo F. Abrantes Ludmila Rodrigues Pinto Ferreira Ricardo T. Gazzinelli

The inflammatory response elicited by various stimuli such as microbial products or cytokines is determined by differences in the pattern of cellular gene expression. We have used the differential display RT-PCR (DDRT-PCR) strategy to identify mRNAs that are differentially expressed in various murine cell types stimulated with pro-inflammatory cytokines, microbial products or anti-inflammatory drugs. Mouse embryonic fibroblasts (MEFs) were treated with IFNs, TNF, or sodium salicylate. Also, peritoneal macrophages from C3H/Hej mice were stimulated with T. cruzi-derived GPI-mucin and/or IFN-g. After DDRT-PCR, various cDNA fragments that were differentially represented on the sequencing gel were recovered, cloned and sequenced. Here, we describe a summary of several experiments and show that, when 16 of a total of 28 recovered fragments were tested for differential expression, 5 (31%) were found to represent mRNAs whose steady-state levels are indeed modulated by the original stimuli. Some of the identified cDNAs encode for known proteins that were not previously associated with the inflammatory process triggered by the original stimuli. Other cDNA fragments (8 of 21 sequences, or 38%) showed no significant homology with known sequences and represent new mouse genes whose characterization might contribute to our understanding of inflammation. In conclusion, DDRT-PCR has proven to be a potent technology that will allow us to identify genes that are differentially expressed when cells are subjected to changes in culture conditions or isolated from different organs.

Differential display

10.1590/s0100-879x1999000700008

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No-match ORESTES explored as tumor markers

Nucleic Acids Research (2009)

Barbara P. Mello Eduardo F. Abrantes César Torres Ariane Machado‐Lima Rogério da Silva Fonseca

Sequencing technologies and new bioinformatics tools have led to the complete sequencing of various genomes. However, information regarding the human transcriptome and its annotation is yet to be completed. The Human Cancer Genome Project, using ORESTES (open reading frame EST sequences) methodology, contributed to this objective by generating data from about 1.2 million expressed sequence tags. Approximately 30% of these sequences did not align to ESTs in the public databases and were considered no-match ORESTES. On the basis that a set of these ESTs could represent new transcripts, we constructed a cDNA microarray. This platform was used to hybridize against 12 different normal or tumor tissues. We identified 3421 transcribed regions not associated with annotated transcripts, representing 83.3% of the platform. The total number of differentially expressed sequences was 1007. Also, 28% of analyzed sequences could represent noncoding RNAs. Our data reinforces the knowledge of the human genome being pervasively transcribed, and point out molecular marker candidates for different cancers. To reinforce our data, we confirmed, by real-time PCR, the differential expression of three out of eight potentially tumor markers in prostate tissues. Lists of 1007 differentially expressed sequences, and the 291 potentially noncoding tumor markers were provided.

10.1093/nar/gkp074

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Citations (10)

Su1607 Screening for Colorectal Cancer: Results of a Pilot Population-Based Screening Program in the Brazilian Amazon Region

Gastrointestinal Endoscopy (2016)

Ângelo Paulo Ferrari Fernanda Prata Martins Eduardo F. Abrantes Aldenir Fresca Cristiana M. Toscano

Colorectal cancer screening

10.1016/j.gie.2016.03.922

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Identification, structural characterization, and tissue distribution of Tsg-5: a new TNF-stimulated gene

Genes and Immunity (2003)

Eduardo F. Abrantes Eliane Pires Andreia F. Carvalho F. F. Costa Wilson Savino

Exon trapping

10.1038/sj.gene.6363949

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