This data comprises a total of 213 cases evaluated for Ebola virus infection at the Kenema Government Hospital in Sierra Leone between May 25 and June 18, 2014. Outcome data was available for 87 of 106 EBOV positive cases. Metabolic panels were performed on 98 Ebola virus disease and non-Ebola virus disease illness patients with adequate samples volumes. Ebola virus load was determined in 63 cases with adequate samples volumes by quantitative polymerase chain reaction (qPCR) at Harvard University. Sign and symptom data was obtained on 44 patients with a clinical chart that were admitted to Kenema Hospital. The metabolic panels were obtained from serum samples analyzed with a Piccolo Blood Chemistry Analyzer and Comprehensive Metabolic Reagent Discs (Abaxis).

10.7910/dvn/29296

Cite

Citations (0)

Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak

Science (2014)

Stephen Gire Augustine Goba Kristian G. Andersen Rachel Sealfon Daniel J. Park

In its largest outbreak, Ebola virus disease is spreading through Guinea, Liberia, Sierra Leone, and Nigeria. We sequenced 99 Ebola virus genomes from 78 patients in Sierra Leone to ~2000× coverage. We observed a rapid accumulation of interhost and intrahost genetic variation, allowing us to characterize patterns of viral transmission over the initial weeks of the epidemic. This West African variant likely diverged from central African lineages around 2004, crossed from Guinea to Sierra Leone in May 2014, and has exhibited sustained human-to-human transmission subsequently, with no evidence of additional zoonotic sources. Because many of the mutations alter protein sequences and other biologically meaningful targets, they should be monitored for impact on diagnostics, vaccines, and therapies critical to outbreak response.

Ebolavirus

10.1126/science.1259657

Cite

Citations (1,237)

Clinical Illness and Outcomes in Patients with Ebola in Sierra Leone

New England Journal of Medicine (2014)

John S. Schieffelin Jeffrey G. Shaffer Augustine Goba Michael Gbakie Stephen Gire

Limited clinical and laboratory data are available on patients with Ebola virus disease (EVD). The Kenema Government Hospital in Sierra Leone, which had an existing infrastructure for research regarding viral hemorrhagic fever, has received and cared for patients with EVD since the beginning of the outbreak in Sierra Leone in May 2014.

Case fatality rate

10.1056/nejmoa1411680

Cite

Citations (513)

An Outbreak of Ebola Virus Disease in the Lassa Fever Zone

The Journal of Infectious Diseases (2016)

Augustine Goba Sheik Humarr Khan Mbalu Fonnie Mohamed Fullah Alex Moigboi

Background. Kenema Government Hospital (KGH) has developed an advanced clinical and laboratory research capacity to manage the threat of Lassa fever, a viral hemorrhagic fever (VHF). The 2013–2016 Ebola virus (EBOV) disease (EVD) outbreak is the first to have occurred in an area close to a facility with established clinical and laboratory capacity for study of VHFs. Methods. Because of its proximity to the epicenter of the EVD outbreak, which began in Guinea in March 2014, the KGH Lassa fever Team mobilized to establish EBOV surveillance and diagnostic capabilities. Results. Augustine Goba, director of the KGH Lassa laboratory, diagnosed the first documented case of EVD in Sierra Leone, on 25 May 2014. Thereafter, KGH received and cared for numbers of patients with EVD that quickly overwhelmed the capacity for safe management. Numerous healthcare workers contracted and lost their lives to EVD. The vast majority of subsequent EVD cases in West Africa can be traced back to a single transmission chain that includes this first diagnosed case. Conclusions. Responding to the challenges of confronting 2 hemorrhagic fever viruses will require continued investments in the development of countermeasures (vaccines, therapeutic agents, and diagnostic assays), infrastructure, and human resources.

Lassa fever

Viral hemorrhagic fever

Lassa virus

Ebolavirus

Ebola Hemorrhagic Fever

10.1093/infdis/jiw239

Cite

Citations (36)