Cheng‐Kuan Su

National Chung Hsing University

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Yuh‐Chang Sun

National Tsing Hua University

Yu‐Fen Huang

BGI Group (China)

Chih‐Min Wang

National Taiwan Ocean University

Po‐Wen Chung

Institute of Chemistry, Academia Sinica

John L. Fulton

Pacific Northwest National Laboratory

Joseph Nagyváry

Texas A&M University

Hsiao‐Han Tseng

National Taiwan University

Yong‐Jie Lin

Southwest University of Science and Technology

Hsien-Ting Chiu

National Tsing Hua University

Chien‐Sheng Chen

National Cheng Kung University

Cooperative Institutions

National Tsing Hua University

National Taiwan Ocean University

National Taiwan University

National Chung Hsing University

National Sun Yat-sen University

National Cheng Kung University

University of California, Berkeley

National Hsinchu University of Education

National Central University

National Yang Ming Chiao Tung University

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Enzyme-Immobilized 3D-Printed Reactors for Online Monitoring of Rat Brain Extracellular Glucose and Lactate

Analytical Chemistry (2016)

Cheng‐Kuan Su Shuo-Chih Yen Tzu-Wen Li Yuh‐Chang Sun

In this study we constructed a highly sensitive system for in vivo monitoring of the concentrations of rat brain extracellular glucose and lactate. This system involved microdialysis (MD) sampling and fluorescence determination in conjunction with a novel sample derivatization scheme in which glucose oxidase and lactate oxidase were immobilized in ABS flow bioreactors (manufactured through low-cost three-dimensional printing (3DP)), via fused deposition modeling, for online oxidization of sampled glucose and lactate, respectively, in rat brain microdialysate. After optimizing the experimental conditions for MD sampling, the manufacture of the designed flow reactors, the enzyme immobilization procedure, and the online derivatization scheme, the available sampling frequency was 15 h–1 and the system's detection limits reached as low as 0.060 mM for glucose and 0.059 mM for lactate, based on a 20-μL conditioned microdialysate; these characteristics were sufficient to reliably determine the concentrations of extracellular glucose and lactate in the brains of living rats. To demonstrate the system's applicability, we performed (i) spike analyses of offline-collected rat brain microdialysate and (ii) in vivo dynamic monitoring of the extracellular glucose and lactate in living rat brains, in addition to triggering neuronal depolarization by perfusing a high-K+ medium from the implanted MD probe. Our analytical results and demonstrations confirm that postprinting functionalization of analytical devices manufactured using 3DP technology can be a powerful strategy for extending the diversity and adaptability of currently existing analytical configurations.

Microdialysis

Glucose oxidase

10.1021/acs.analchem.6b00272

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Citations (48)

Online open-tubular fractionation scheme coupled with push–pull perfusion sampling for profiling extravasation of gold nanoparticles in a mouse tumor model

Journal of Chromatography A (2015)

Cheng‐Kuan Su Po‐Jen Tseng Meng‐Han Lin Hsien-Ting Chiu Andrea del Vall

Extravasation

PEGylation

10.1016/j.chroma.2015.05.008

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Citations (3)

Comparative Protective Action of Unsaturated Fatty Acids for Mice Against Exotoxin, Endotoxin and Snake Venom.

Experimental Biology and Medicine (1963)

Wesley W. Spink Cheng‐Kuan Su

Swiss-Webster mice were protected against lethal amounts of exotoxins prepared from Staphylococcus, Cl. perfringens, and Cl. tetani when the toxins were mixed with oleic or linoleic unsaturated fatty acids prior to intravenous injection. Injection of a toxin and unsaturated fatty acid separately also gave protection. Unsaturated fatty acid also protected against Crotalus terrificus snake venom. No protection was obtained with oleic acid against E. coli or Br. melitensis endotoxin.

Exotoxin

Unsaturated fatty acid

10.3181/00379727-112-28078

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In-vivo evaluation of the permeability of the blood–brain barrier to arsenicals, molybdate, and methylmercury by use of online microdialysis–packed minicolumn–inductively coupled plasma mass spectrometry

Analytical and Bioanalytical Chemistry (2013)

Cheng‐Kuan Su Cheng-Hung Yang Cheng-Hsing Lin Yuh‐Chang Sun

Microdialysis

10.1007/s00216-013-7429-5

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Citations (17)

Bioprosthesis of Core–Shell Gold Nanorod/Serum Albumin Nanoimitation: A Half-Native and Half-Artificial Nanohybrid for Cancer Theranostics

Chemistry of Materials (2017)

Hsien-Ting Chiu Chung‐Hao Chen Menglin Li Cheng‐Kuan Su Yuh‐Chang Sun

High concentrations of aldehyde-based cross-linkers have been commonly used for protein immobilization to facilitate microscale and nanoscale observations. This fixation maintains cell morphology and partial protein activity. In this study, a facile one-step strategy based on a similar concept was first developed for the bioprosthesis of a uniform core–shell gold nanorod/serum albumin (NR@SA) nanoplatform. The resultant albumin shell preserved half of its native form, leading to decreased free SA adsorption, and even these adsorbed proteins were close to their native form. This strategy efficiently prevents subsequent adsorption cascades of other proteins and has a remarkable influence on cellular uptake (of macrophages and tumor cells). Furthermore, the other, artificial part endowed NR@SAs with higher drug loading capacity and enhanced photoacoustic signal intensity for cancer theranostics compared with those of its pristine counterpart. These findings suggested that preserved fidelity and artificial characterizations provide a new perspective for biomimetic nanomaterial design.

Nanorod

Nanomaterials

Microscale chemistry

10.1021/acs.chemmater.7b04127

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Citations (20)

A Unique Diel Pattern in Carbonate Chemistry in the Seagrass Meadows of Dongsha Island: The Enhancement of Metabolic Carbonate Dissolution in a Semienclosed Lagoon

Frontiers in Marine Science (2021)

Wen-Chen Chou Lan‐Feng Fan Chang-Chang Yang Ying-Hsuan Chen Chin‐Chang Hung

In contrast to other seagrass meadows where seawater carbonate chemistry generally shows strong diel variations with higher pH but lower partial pressure of CO 2 ( p CO 2 ) during the daytime and lower pH but higher p CO 2 during nighttime due to the alternation in photosynthesis and respiration, the seagrass meadows of the inner lagoon (IL) on Dongsha Island had a unique diel pattern with extremely high pH and low p CO 2 across a diel cycle. We suggest that this distinct diel pattern in pH and p CO 2 could be associated with the enhancement of total alkalinity (TA) production coupled to carbonate sediment dissolution in a semienclosed lagoon. The confinement of the IL may hamper water exchange and seagrass detritus export to the adjacent open ocean, which may result in higher organic matter loading to the sediments, and longer residence time of the water in the IL, accompanied by microbial respiration (both aerobic and anaerobic) that may reduce carbonate saturation level to drive carbonate dissolution and thus TA elevation, thereby forming such a unique diel pattern in carbonate chemistry. This finding further highlights the importance of considering TA production through metabolic carbonate dissolution when evaluating the potential of coastal blue carbon ecosystems to buffer ocean acidification and to absorb atmospheric CO 2 , in particular in a semienclosed setting.

Diel vertical migration

Bioturbation

Carbonate minerals

10.3389/fmars.2021.717685

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Citations (13)

3D-Printed Column with Porous Monolithic Packing for Online Solid-Phase Extraction of Multiple Trace Metals in Environmental Water Samples

Analytical Chemistry (2020)

Cheng‐Kuan Su Jou-Yu Lin

In this study, we used a multimaterial three-dimensional printing (3DP) technology and porous composite filaments (Lay-Fomm, Gel-Lay, and Lay-Felt) to fabricate solid phase extraction (SPE) columns for the enhanced extraction of multiple metal ions. When employed as sample pretreatment devices in an automatic flow injection analysis/inductively coupled plasma mass spectrometry (ICP-MS) system, these 3D-printed SPE columns performed the near-complete extractions of Mn, Co, Ni, Cu, Zn, Cd, and Pb ions from natural water samples prior to ICP-MS determination. After optimizing the column fabrication, the extraction conditions, and the automatic analysis system, the column packed with the porous composite Lay-Fomm 40 was found to provide the highest extraction performance—the extraction efficiencies of the listed metal ions were all greater than 99.2%, and the detection limits of the method ranged from 0.3 to 6.7 ng L–1. The detection of these metal ions in several reference materials (CASS-4, SLEW-3, 1640a, and 1643f) validated the reliability of this method; spike analyses of collected water samples (groundwater, river water, and seawater) demonstrated the applicability of the method. The nature of the printing materials enhanced the analytical performance of 3D-printed sample pretreatment devices. Such approaches will be useful to diversify the range of sample preparation schemes and analytical methods enabled by 3DP technologies.

Solid phase extraction

Sample Preparation

10.1021/acs.analchem.0c00863

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Citations (57)

3D-printed thermally expanded monolithic foam for solid-phase extraction of multiple trace metals

Microchimica Acta (2024)

Yu-Hsuan Cheng Cheng‐Kuan Su

TRACE (psycholinguistics)

Solid phase extraction

10.1007/s00604-024-06691-9

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Citations (2)

CCDC 1822766: Experimental Crystal Structure Determination

The Cambridge Structural Database (2018)

Chih‐Min Wang Yong‐Jie Lin Ming‐Feng Pan Cheng‐Kuan Su Tai‐Yuan Lin

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

10.5517/ccdc.csd.cc1z5qxs

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Citations (0)

4D-Printed Temperature-Controlled Flow-Actuated Solid-Phase Extraction Devices

Analytical Chemistry (2021)

Chun‐Yi Wu Jyun-Ran Chen Cheng‐Kuan Su

Four-dimensional printing (4DP) technologies can extend the functionality and applicability of manufactured analytical devices through employing stimuli-responsive materials. In this study, we used a photocurable resin of stimuli-responsive shape-memory polymers and digital light processing three-dimensional printing (3DP) to fabricate a smart sample pretreatment device featuring a solid-phase extraction (SPE) column and a temperature-controlled flow-actuated valve. Through manipulation of the temperatures and flow rates of the sample, eluent, and rinsing streams, we used this 4D-printed SPE device to extract Mn, Co, Ni, Cu, Zn, Cd, and Pb ions from high-salt content samples and remove the sample matrix prior to their determination by inductively coupled plasma mass spectrometry. After optimizing the valve design and operation and the analytical scheme, this device displayed competitive analytical performance—the method detection limits (MDLs) ranged from 0.7 to 22.1 ng L–1 for these metal ions (the MDLs ranged from 0.5 to 18.8 ng L–1 when validating the same printed SPE column using an online automatic system equipped with electric switching valves). Furthermore, we performed analyses of these metal ions in three reference materials (CASS-4, 1643f, and 2670a) and spike analyses of collected samples (seawater, ground water, river water, and human urine) to confirm the reliability and applicability of this analytical method. For the first time, 4DP has been used to fabricate a multi-functional, stimuli-responsive sample pretreatment device displaying analytical performance equal to that of a commercial apparatus. This novel approach builds upon the functionality and diversity of 3DP-enabling devices with the goal of developing more efficient analytical schemes.

Solid phase extraction

Matrix (chemical analysis)

10.1021/acs.analchem.1c01703

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Citations (18)