Irene Fernandes

Universidade de Itaúna

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H.A. Takehara

Instituto Butantan

Mário Abbud‐Filho

Faculdade de Medicina de São José do Rio Preto

Heloisa Cristina Caldas

Hospital de Base

Ana Maria Moura‐da‐Silva

Instituto Butantan

Maria Alice Sperto Ferreira Baptista

Hospital de Base

Isabelle Tanjoni

Instituto Butantan

José Marı́a Gutiérrez

Universidad de Costa Rica

Eny Maria Goloni‐Bertollo

Faculdade de Medicina de São José do Rio Preto

Eliana L. Faquim‐Mauro

Instituto Butantan

Roxane Maria Fontes Piazza

Instituto Butantan

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Instituto Butantan

116

Universidade de São Paulo

Faculdade de Medicina de São José do Rio Preto

Universidade Brasil

Universidade Federal de São Paulo

Hospital de Base

Universidade Estadual de Campinas (UNICAMP)

Instituto de Ciências Farmacêuticas

Universidade Federal de Minas Gerais

Hospital Universitário da Universidade de São Paulo

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Serological and molecular characterization of AdaA: a potential marker of Q-fever abortion in goats?

Clinical Microbiology and Infection (2009)

Irene Fernandes Élodie Rousset P. Dufour Karim Sidi‐Boumedine A. Cupo

Coxiella burnetii

Q fever

Primer (cosmetics)

10.1111/j.1469-0691.2008.02209.x

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Citations (2)

Taxonomia dos representantes de Cyatheaceae do nordeste oriental do Brasil

Pesquisas. Botanica (2003)

Irene Fernandes

Source

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Citations (41)

Neutrophils do not contribute to local tissue damage, but play a key role in skeletal muscle regeneration, in mice injected with Bothrops asper snake venom

Muscle & Nerve (2003)

Catarina Teixeira Stella Regina Zamunér Juliana P. Zuliani Cristina María Fernándes M.A. Cruz-Höfling

Abstract Local tissue damage induced by crotaline snake venoms includes edema, myonecrosis, hemorrhage, and an inflammatory response associated with a prominent cellular infiltrate. The role of neutrophils in the local tissue damage induced by Bothrops asper snake venom and by myotoxin I, a phospholipase A 2 isolated from this venom, was investigated. Male Swiss mice were pretreated with either an antimouse granulocyte rat monoclonal immunoglobulin G (IgG) antibody or with isotype‐matched control antibody. No significant differences in these local effects were observed between mice pretreated with antigranulocyte antibodies and those receiving control IgG. Moreover, myotoxicity induced by B. asper myotoxin I was similar in neutrophil‐depleted and control mice. The role of neutrophils in the process of skeletal muscle regeneration was also assessed. Muscle regeneration was assessed by quantifying the muscle levels of creatine kinase and by morphometric histological analysis of the area comprised by regenerating cells in damaged regions of skeletal muscle. Mice depleted of neutrophils and then injected with B. asper venom showed a more deficient regenerative response than mice pretreated with control IgG. Moreover, a drastic difference in the regenerative response was observed in mice injected with myotoxin I, because animals pretreated with control IgG showed a successful regeneration, whereas those depleted of neutrophils had abundant areas of necrotic tissue that had not been removed 7 days after injection, associated with reduced contents of creatine kinase. It is concluded that (1) neutrophils do not play a significant role in the acute local pathological alterations induced by the venom of B. asper , and (2) neutrophils play a prominent role in the process of skeletal muscle regeneration after injection of B. asper venom and myotoxin I, probably related to the phagocytosis of necrotic material and the recruitment of other inflammatory cells, two events directly associated with a successful muscle regenerative response. Muscle Nerve 28: 449–459, 2003

Myotoxin

Creatine kinase

10.1002/mus.10453

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Citations (208)

A rapid and efficient purification method for horse IgG(T) using a rat monoclonal antibody.

PubMed (1994)

Irene Fernandes F. Cormont Dominique Latinne H. Bazin H.A. Takehara

1. Louvain rats (IgK-1a) were immunized with horse IgG(T). To generate mAb to IgG(T), popliteal lymph node cells taken from the immunized animals were fused to a non-secreting LOU/C immunocytoma (IR983F). The hybridomas were cultured in HAT-containing medium and cloned under limiting dilution conditions. Supernatants from the growing hybrids were screened by ELISA using plates coated with horse IgG(T) or IgGa+b+c. 2. The anti-IgG(T) mAb obtained was named LO-HoGT-1 (LOU anti-horse IgG(T)). It is an IgG2a rat antibody whose light chain allotype is IgK-1a, and with an affinity constant of 2.9 x 10(10) M-1. 3. Ascites was induced in LOU (IgK-1b) rats by injecting the hybridoma cells and incomplete Freund's adjuvant ip. To obtain purified mAb, ascitic fluid was applied to a Sepharose anti-rat LOU IgK-1a chain column. 4. The purified mAb was then coupled to Sepharose. Immunoelectrophoretically pure IgG(T) was obtained by passage of horse serum through this column. The entire procedure took less than 30 min and resulted in a highly purified IgG(T).

Sepharose

Allotype

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Citations (9)

Immunomodulatory effects of crotoxin isolated from Crotalus durissus terrificus venom in mice immunised with human serum albumin

Toxicon (2011)

Bruna C. Favoretto Renata Ricardi S.R. Silva Jacqueline F. Jacysyn Irene Fernandes

Crotalus

10.1016/j.toxicon.2010.12.023

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Citations (29)

Production and characterization of a monoclonal antibody against an Ascaris suum allergenic component

Brazilian Journal of Medical and Biological Research (2001)

Roberta Ramos Pires Telma Miyuki Oshiro D. M. Itami Irene Fernandes M.F. Macedo-Soares

Ascaris suum allergenic components (PIII) separated by gel filtration chromatography of an adult worm extract were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells using polyethylene glycol (MW 1450) as fusogen. The hybridomas were cultured in HAT-containing medium and cloned at limiting dilutions. Supernatants from the growing hybrids were screened by ELISA using plates coated with PIII or the A. suum crude extract. The monoclonal antibody obtained, named MAC-3 (mouse anti-A. suum allergenic component), is an IgG1 kappa mouse immunoglobulin that specifically recognizes a 29,000 molecular weight protein (called allergenic protein) with an affinity constant of 1.7 x 10(9) M-1. The A. suum components recognized by MAC-3 induce specific IgE antibody production in immunized BALB/c mice. Ascitic fluid induced in Swiss mice by injecting ip the hybridoma cells and incomplete Freund's adjuvant was purified by affinity chromatography using a protein A-Sepharose column. The purified monoclonal antibody was then coupled to activated Sepharose beads in order to isolate the A. suum allergenic component from the whole extract by affinity chromatography.

Ascaris suum

Sepharose

10.1590/s0100-879x2001000800009

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Citations (12)

Variables associated to fetal microchimerism in systemic lupus erythematosus patients

Clinical Rheumatology (2015)

Greiciane Maria da Silva Florim Heloisa Cristina Caldas Érika Cristina Pavarino Eny Maria Goloni‐Bertollo Irene Fernandes

Microchimerism

10.1007/s10067-015-3122-8

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Citations (7)