Wilton Mitsunari Takeshita

Universidade Estadual Paulista (Unesp)

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Cléverson Luciano Trento

Universidade Federal de Sergipe

Mariliani Chicarelli da Silva

Universidade Estadual de Maringá

Lílian Cristina Vessoni Iwaki

Universidade Estadual de Maringá

Lucas Alves da Mota Santana

Universidade Federal de Sergipe

Marcos Antônio Lima dos Santos

Universidade de São Paulo

Daniel Araki Ribeiro

Universidade Federal de São Paulo

Liogi Iwaki Filho

Universidade Estadual de Maringá

Liane Maciel de Almeida Souza

Universidade Federal de Sergipe

Bruno Natan Santana Lima

Universidade Federal de Sergipe

Liciane dos Santos Menezes

Universidade Federal de Sergipe

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Universidade Federal de Sergipe

156

Universidade Estadual de Maringá

Universidade de São Paulo

Universidade Estadual de Campinas (UNICAMP)

Universidade Estadual Paulista (Unesp)

Universidade Tiradentes

Fundação Faculdade de Odontologia

RELX Group (Netherlands)

Universidade Federal de São Paulo

Universidade Federal da Bahia

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Can exposure to panoramic radiographs induce genotoxic effects on the oral epithelium? A systematic review with meta-analysis

Dentomaxillofacial Radiology (2021)

Marcos Antônio Lima dos Santos Graziane Ribeiro Couto Mark Jon Santana Sabey DANILO DE PAULA RIBEIRO BORGES Wilton Mitsunari Takeshita

Radiographic examinations combined with clinical and histological tests play an important role in diagnosing changes in the craniofacial region. Considering that X-radiation is a form of ionizing energy capable of damaging the DNA of individuals, it has been the object of studies since its introduction. The micronucleus test is used to quantify this damage, assessing whether or not chromosomal breakage occurs. Objective: To evaluate, through a systematic review (SR) with meta-analysis, the occurrence of genotoxic effects in the oral epithelium after the exposure of patients to panoramic radiographs. Methods: An SR was performed with the PICOS (Population, Intervention, Comparison, Outcome, and Study design) strategy, aiming to answer the following question: “Can panoramic radiographs induce genotoxic effects on the oral epithelium?” The study was registered in the PROSPERO (International prospective register of systematic reviews) platform. A systematic search was performed in the following electronic databases: PubMed (including MedLine), Scopus, Embase, LILACS, Medline EbscoHost, and Google Scholar. Treatment effects were defined as standardized mean difference (SMD), and 95% confidence intervals (CI) were established. The Joanna Briggs Institute questionnaire for observational studies was applied to assess the risk of bias. The GRADE tool was used to assess the quality of evidence of the SR. Results: A total of 251 potentially relevant studies were selected through the search strategy. After screening titles and abstracts, 11 full-text manuscripts were assessed for eligibility and nine observational studies were included in the meta-analysis. The present study showed an increase in micronuclei after the exposure (SMD = 0.21, 95% CI, 0.03 to 0.28, p = 0.02), with a Tau 2 index = 0.00, Chi 2 = 2.35, and p-value = 0.97. Therefore, the articles selected were considered homogeneous and the I² of 0% indicated low heterogeneity. Conclusion: According to the studies analysed, although the quality of evidence was considered low, panoramic radiographs can cause genotoxic damage in the oral epithelium but with a small effect size.

10.1259/dmfr.20210149

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An uncommon case of primary parotid tuberculosis in a paediatric patient

Oral Surgery (2020)

Erika Rezende‐Silva Lucas Alves da Mota Santana YASMIN MATOS FREIRE COSTA Lucas Celestino Guerzet Ayres Wilton Mitsunari Takeshita

10.1111/ors.12556

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The use of micronucleus assay in exfoliated oral cells in patients undergoing fixed orthodontic therapy: a systematic review with meta-analysis

Brazilian Oral Research (2023)

Daniel Vitor de Souza Wilton Mitsunari Takeshita Gláucia Monteiro de Castro Ana Claudia Muniz Renno Jean Nunes dos SANTOS

The aim of this systematic review was to evaluate published papers regarding the micronucleus assay in oral mucosal cells of patients undergoing orthodontic therapy (OT). A search of the scientific literature was made in the PubMed, Scopus, and Web of Science databases for all data published until November, 2021 using the combination of the following keywords: “fixed orthodontic therapy,” “genetic damage”, “DNA damage,” “genotoxicity”, “mutagenicity”, “buccal cells”, “oral mucosa cells,” and “micronucleus assay”. The systematic review was designed according to the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines. Nine studies were retrieved. Some authors demonstrated that OT induces cytogenetic damage in oral mucosal cells. Out of the nine studies included, two were classified as strong, five as moderate, and two as weak, according to the quality assessment components of the Effective Public Health Practice Project (EPHPP). Meta-analysis data revealed no relationship between mutagenicity in oral cells and OT in different months of treatment. At one month, the SMD = 0.65 and p = 0.08; after three months of OT, the SMD = 1.21 and p = 0.07; and after six months of OT, the SMD = 0.56 and p = 0.11. In the analyzed months of OT, I2 values were >75%, indicating high heterogeneity. In summary, this review was not able to demonstrate that OT induces genetic damage in oral cells. The study is important for the protection of patients undergoing fixed OT, given that mutagenesis participates in the multi-step process of carcinogenesis.

Web of science

Buccal swab

10.1590/1807-3107bor-2023.vol37.0116

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Assessment of anesthetic properties and pain during needleless jet injection anesthesia: a randomized clinical trial

Figshare (2019)

Allan Carlos Araújo de Oliveira Klinger de Souza Amorim Edmundo Marquês do Nascimento Júnior Amanda Caroline Batista Duarte Francisco Carlos Groppo

Abstract Pain due to administration of local anesthetics is the primary reason for patients' fear and anxiety, and various methods are used to minimize it. This study aimed to measure the degree of pain during administration of anesthesia and determine the latency time and duration of pulpal anesthesia using two anesthetic methods in the maxilla. Materials and Methods: A randomized, single-blind, split-mouth clinical trial was conducted with 41 volunteers who required class I restorations in the maxillary first molars. Local anesthesia was administered with a needleless jet injection system (experimental group) or with a carpule syringe (control) using a 30-gauge short needle. The method of anesthesia and laterality of the maxilla were randomized. A pulp electric tester measured the latency time and duration of anesthesia in the second molar. Visual analogue scale (VAS) was used to measure the degree of pain during the anesthetic method. Data were tabulated and then analyzed by a statistician. The t-test was used to analyze the differences between the groups for basal electrical stimulation. Duration of anesthesia and degree of pain were compared using the Mann-Whitney test. A 5% significance level was considered. Results: There was no statistical difference in the basal electrical stimulation threshold (mA) and degree of pain between the two methods of anesthesia (p>0.05). Latency time was 2 minutes for all subjects. The duration of pulpal anesthesia showed no statistical difference (minutes) between the two methods (p<0.001), with a longer duration for the traditional method of anesthesia (median of 40 minutes). Conclusions: The two anesthetics methods did not differ concerning the pain experienced during anesthesia. Latency lasted 2 minutes for all subjects; the traditional infiltration anesthesia resulted in a longer anesthetic duration compared with the needleless jet injection.

10.6084/m9.figshare.7647332

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Radiographic analysis of 1000 cast posts in Sergipe state, Brazil

Figshare (2017)

Caroline Garcez MENDONÇA José Rogério Vieira de Almeida Wilton Mitsunari Takeshita Fábio Martins Mônica Silveira Paixão

Abstract Introduction Endodontically treated teeth with extensive coronary destruction require posts and cores to enable prosthetic restoration. Despite the increasing use of prefabricated posts, cast metal posts and cores are still widely used. The quality of the latter is important for the longevity of restorative treatment, and failure can occur if the fundamental principles are not followed. Objective To radiographically evaluate the prosthetic principles of 1000 cast metal post-and-core restorations performed in single-rooted teeth and their coherence with the principles used for their confection. Material and method Digital periapical radiographs of 1000 endodontically treated, single-rooted teeth with cast metal posts and cores were selected from the collection of a radiology clinic (Centro de Imagem, Aracaju/SE, Brazil). The images were analyzed using a measurement software (ImageJ, USA) in accordance with the fundamental prosthetic principles: length and diameter of the post, ratio between post and bone crest, contiguity of post to the root canal, gap between post and the remaining root canal filling, amount of remaining root canal filling, and absence of periapical lesion. The data were qualitatively analyzed, classified into ideal and not ideal, and submitted to the chi-square test (α=0.05). Result Only 6.7% of the cast metal posts analyzed were satisfactorily fabricated. Conclusion Many prosthetic criteria are neglected during the manufacturing of cast metal post and cores, resulting in inadequate work that may compromise the longevity of restorative treatments.

10.6084/m9.figshare.5667310

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Guia de orientação sobre osteorradionecrose dos maxilares

Mariliani Chicarelli da Silva Wilton Mitsunari Takeshita

10.4025/9788576287698

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Artificial intelligence-based cephalometric landmark annotation and measurements according to Arnett’s analysis: can we trust a bot to do that?

Dentomaxillofacial Radiology (2021)

Thaísa Pinheiro Silva Mariana Mendonça Hughes Liciane dos Santos Menezes MARIA DE FÁTIMA BATISTA DE MELO Paulo Henrique Luiz de Freitas

To assess the reliability of CEFBOT, an artificial intelligence (AI)-based cephalometry software, for cephalometric landmark annotation and linear and angular measurements according to Arnett's analysis.Thirty lateral cephalometric radiographs acquired with a Carestream CS 9000 3D unit (Carestream Health Inc., Rochester/NY) were used in this study. The 66 landmarks and the 10 selected linear and angular measurements of Arnett's analysis were identified on each radiograph by a trained human examiner (control) and by CEFBOT (RadioMemory Ltd., Belo Horizonte, Brazil). For both methods, landmark annotations and measurements were duplicated with an interval of 15 days between measurements and the intraclass correlation coefficient (ICC) was calculated to determine reliability. The numerical values obtained with the two methods were compared by a t-test for independent variables.CEFBOT was able to perform all but one of the 10 measurements. ICC values > 0.94 were found for the remaining eight measurements, while the Frankfurt horizontal plane - true horizontal line (THL) angular measurement showed the lowest reproducibility (human, ICC = 0.876; CEFBOT, ICC = 0.768). Measurements performed by the human examiner and by CEFBOT were not statistically different.Within the limitations of our methodology, we concluded that the AI contained in the CEFBOT software can be considered a promising tool for enhancing the capacities of human radiologists.

Landmark

Cephalometric analysis

10.1259/dmfr.20200548

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Citations (30)

Effect of intensity of light sources and barriers in ceramic hardness of dual-cured cement

Figshare (2018)

Gabriela Santos Fonseca Ayla Macyelle de Oliveira Correia Sandro Griza Milko Villarroel Wilton Mitsunari Takeshita

AbstractIntroductionThe dual-cure resin cement is the main material for luting of ceramic. However, factors such as light source can influence the performance of the cement.ObjectiveEvaluate the influence of two light sources of different intensities on the microhardness of a resin cement when cured under different ceramics.Material and methodForty samples of dual resin cement were photoactivated by two LED light sources, with light intensity of 1000mW/cm² and 800mW/cm², respectively (40 seconds), under the base ceramic Zirconia, Spinel MgAl2O4 and Lithium Disilicate. In the control group, a glass plate was used. Each samples had the surface grounded (600 and 1200). After dry storage in the dark (24 hours), Vickers microhardness measurements were performed (HMV Shimadzu - 50gf/10 seconds). The data were submitted to factorial ANOVA and Tukey's test with significance level of 5%.ResultThe average hardness and standard deviation in the control group for cement polymerized 1000mW/cm² was 43.9±1.5 and 800mW/cm² the value of 43.4±1.3. The lowest value was for the Ceramic base of spinel MgAl2O4, light cured with 800mW/cm² with a value of 34.3±3.3.ConclusionThe ceramic type and intensity of the light source influence the hardness of the cement. The polymerization 1000mW/cm2 on the ceramic based on zirconia and spinel MgAl2O4 resulted in the best combination of hardness cement.

Intensity

10.6084/m9.figshare.7515566

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Oral lesions induced by COVID‐19 vaccines: A critical analysis of the histological findings

Oral Surgery (2024)

Marina dos Santos Barreto Lucas Alves da Mota Santana Kaio Victtor dos Santos Barbosa Alexia de Santana Oliveira Gina Delia Roque‐Torres

The data sets generated and/or analysed during the current study are available from the corresponding author upon reasonable request.

2019-20 coronavirus outbreak

10.1111/ors.12923

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Knowledge assessment of dentistry undergraduate students and dentists in the city of Aracaju/ Sergipe regarding the appropriate use of antimicrobial drugs

Figshare (2018)

Cléverson Luciano Trento Laerte Ribeiro Menezes Júnior Allancardi dos Santos Siqueira Wilton Mitsunari Takeshita

INTRODUCTION:Antimicrobial agents are a striking example of the evolution of modern medicine. However the adaptability of microorganisms provides resistance to certain antimicrobial drugs.OBJECTIVE:To assess the knowledge of dentistry undergraduate students and dentists in the city of Aracaju, Sergipe State, about antibiotic therapy, thus providing an opportunity to identify and recognize the risks related to improper administration of these drugs.MATERIAL AND METHOD:This is an observational, cross sectional study based on a questionnaire given to 80 undergraduate students of dentistry and 50 dentists, ramdomly chosen, to assess the knowledge on the matter.RESULT: Among the interviewees, about 40% were dentists and 60% were dentistry students, 40% of respondents reported to prescribe antimicrobials using as selection criteria that it be selective and bactericidal, and 90% of respondents said they prescribe antibiotics only when necessary, in order to avoid bacterial resistance.CONCLUSION: According to the methodology applied it can be concluded that both groups showed lack of knowledge about the difference between antibiotic therapy, antimicrobial prophylaxis and drug therapy.

10.6084/m9.figshare.7506293

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