Ajmal Khan

University of Nizwa

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i-10 index

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Co-Authors

Ahmed Al‐Harrasi

University of Nizwa

Muhammad Khisroon

University of Peshawar

Muhammad Khisroon

University of Peshawar

Sobia Ahsan Halim

University of Nizwa

Najeeb Ur Rehman

University of Nizwa

Javeed Farooqi

University of Peshawar

Umar Farooq

COMSATS University Islamabad

Ahmadullah Ahmadullah

Lady Reading Hospital

Farrah Zaidi

University of Peshawar

Abdur Rauf

University of Swabi

Cooperative Institutions

University of Peshawar

University of Nizwa

University of Karachi

COMSATS University Islamabad

The University of Agriculture, Peshawar

Abdul Wali Khan University Mardan

Abbottabad University of Science and Technology

International Center for Chemical and Biological Sciences

Bahauddin Zakariya University

University of Malakand

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Comet assay based DNA evaluation of fuel filling stations and automobile workshops workers from Khyber Pakhtunkhwa province, Pakistan

Journal of Occupational Medicine and Toxicology (2015)

Muhammad Khisroon Aisha Gul Ajmal Khan Naheed Ali Farah Zaidi

Fuel filling stations workers and automobile workshops mechanics are consistently exposed to gasoline hydrocarbons during their occupation, this may cause DNA damage. Objective of this study was to evaluate the level of DNA damage in subjects occupationally exposed to these hydrocarbons. Comet assay was performed on blood lymphocytes of exposed subjects to assess the probable DNA damage. 100 cells per individual were scored and graded by comet tail length. Exposed group consisted of 98 subjects (age 25.4 ± 7.2 years), of which 68 were CNG/Petrol filling men and 30 were automobile workshop workers, selected randomly from different service stations and automobile workshops of populated and adjacent cities of Peshawar, Mardan and Nowshera of Khyber Pakhtunkhwa province, while control group included 92 subjects (age 26.7 ± 11.8 years) were also from the same areas. Significantly high level of DNA damage was found in the subjects exposed to gasoline hydrocarbons as compared to control subjects (173.2 ± 50.1 and 61.0 ± 25.0, P = 0.001, respectively). Period of exposure and use of tobacco also showed considerable effects (P < 0.05) on DNA damage, while effect of age and daily working hours on total comet score (TCS) were non-significant (P > 0.05). The results of our study concluded that petroleum hydrocarbons have the potential to cause DNA damage in the exposed subjects. The study also suggested that protective strategies should be implemented by the concerned authorities to minimize exposure to fuel hydrocarbons.

Comet Assay

Khyber pakhtunkhwa

10.1186/s12995-015-0069-2

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Citations (16)

Prevalence of potentially inappropriate medications use and associated risk factors among elderly cardiac patients using the 2015 American Geriatrics Society beers criteria

Drugs & Therapy Perspectives (2020)

Muhammad Saqlain Zaheer Ahmed Sanan Amjad Butt Ajmal Khan Ali Ahmed

Beers Criteria

Guideline

Pharmacotherapy

10.1007/s40267-020-00747-5

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Citations (2)

Morpholine-tethered Novel Hydrazones as Promising Non-peptidic Prolyl Oligopeptidase (POP) Inhibitors: Synthesis In Vitro and In Silico Studies

Current Medicinal Chemistry (2024)

Urwa Khalid Noor Fatima Naeem Ullah Sobia Ahsan Halim Ajmal Khan

Introduction: Prolyl oligopeptidase (POP) is a pivotal druggable target implicated in diverse biological processes and linked to the development of various ailments, including neurodegenerative disorders. While conventional peptide-based inhibitors have been a centerpiece, their limitations, such as restricted bioavailability, necessitate exploration of non-peptidic inhibitors for their therapeutic potential. Method: This study focuses on designing, synthesizing, and assessing morpholine-based hydrazones targeting the catalytic serine residue of POP. The hydrazones (5a-o), reported as moderately potent analogs compared to the renowned Z-Pro-Prolinal, demonstrated in vitro POP inhibition with IC50 values ranging from 13.60 ± 2.51 to 36.51 ± 1.82 μM. The derivative 5h, with an IC50 of 13.60 ± 2.51 μM, emerged as the most potent inhibitor. Results: Moreover, the in vitro kinetic study of compound 5h indicated that it exhibited concentration-dependent type of inhibition. in silico docking studies of 5h revealed robust interactions in the POP enzyme's active site, yielding a docking score of ˗6.30 Kcal/- mol, consistent with experimental results. Conclusion: All findings underscored the potential of synthesized derivatives for drug development.

Oligopeptidase

Morpholine

Druggability

Docking (animal)

IC50

ADME

10.2174/0109298673304532240911070202

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Bioaccumulation of Heavy Metals in Water, Sediments, and Tissues and Their Histopathological Effects on Anodonta cygnea (Linea, 1876) in Kabul River, Khyber Pakhtunkhwa, Pakistan

BioMed Research International (2018)

Muhammad Iftikhar Khan Muhammad Khisroon Ajmal Khan Naila Gulfam Muhammad Siraj

The present investigation aimed to assess the concentrations of selected heavy metals in water and sediments and their bioaccumulation in tissues of freshwater mussels and their histopathological effects on the digestive gland, gills, and gonads of Anodonta cygnea . Water, sediments, and freshwater mussel samples were collected at four sites, that is, reference and polluted sites, along the Kabul River, Khyber Pakhtunkhwa. The polluted sites were receiving effluents from the industrial, agricultural, municipal, and domestic sources. The order of metals in the water was Zn>Pb>Ni>Cu>Mn>Fe>Cr>Cd, in sediments the order was Fe>Zn>Cr>Ni>Mn>Pb>Cu>Cd, and in the soft tissues the order was Fe>Zn>Mn>Pb>Cu>Cr>Ni>Cd. Histopathological alterations observed in polluted sites of Kabul River were inflammation, hydropic vacuolation, and lipofuscin pigments (in digestive gland), gill lamellar fusion, dilated hemolymphatic sinus, clumping, and generation of cilia and hemocytic infiltration (in gills), and atresia, necrosis, granulocytoma, hemocytic infiltration, and lipofuscin pigments (in gonads). The histopathological alterations in the organs of Anodonta cygnea can be considered as reliable biomarkers in biomonitoring of heavy metal pollution in aquatic ecosystems.

10.1155/2018/1910274

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Citations (44)

Isolation and characterisation of three new anthraquinone secondary metabolites from Symplocos racemosa

Natural Product Research (2015)

Umar Farooq Sadia Naz Ajmal Khan Sara Khan Afsar Khan

Three new anthraquinone secondary metabolites were isolated from Symplocos racemosa, a small tree of family symplocaceae. The structures of compounds (1–3) were elucidated to be 1,4-dihydroxy-6-(ethoxymethyl)-8-propylanthracene-9,10-dione (1), 1,4-dihydroxy-6-(hydroxymethyl)-8-butylanthracene-9,10-dione (2) and 1,4-dihydroxy-6-(hydroxymethyl)-8-propyl anthracene-9,10-dione (3) using their spectral data, i.e. through IR, UV, 1H NMR, 13C NMR and two-dimensional (2D) NMR techniques including heteronuclear multiple quantum coherence, heteronuclear multiple bond correlation and correlation spectroscopy.

Heteronuclear molecule

Hydroxymethyl

Anthraquinones

10.1080/14786419.2015.1046133

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Citations (3)

Three new anthraquinone derivatives isolated from Symplocos racemosa and their antibiofilm activity

Chinese Journal of Natural Medicines (2017)

Umar Farooq Sara Khan Sadia Naz Ajmal Khan Afsar Khan

Anthraquinones

10.1016/s1875-5364(18)30011-6

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Citations (22)

Evaluation of DNA damage in lymphocytes of radiology personnel by comet assay

Sangyo Igaku (2015)

Muhammad Khisroon Ajmal Khan Maryam Naseem Naheed Ali Sardar Khan

Evaluation of DNA damage in lympho- cytes of radiology personnel by comet assay: Muhammad KHISROON, et al. Department of Zoology, University of Peshawar, Pakistan—Objectives: The importance of X-rays as a diagnostic medical tool cannot be denied. However, continuous exposure to X-rays can cause DNA damage. This study aimed to use the comet assay technique to investigate the level of DNA damage in lymphocytes due to X-rays in occupationally exposed personnel. Methods: Blood samples were collected from 74 exposed and 70 control subjects for analysis. A total of 100 randomly captured cells from each slide were examined using an epifluorescent microscope. The comets were analyzed by a visual scoring method according to comet tail length. Results: The results indicated a significant increase (p<0.05) in DNA damage in X-rays technicians (129.8 ± 17.2) as compared with the control group (53.0 ± 25.0). A significant increase (p<0.02) in DNA damage was also observed with an increase in exposure duration of technicians because of their service length. Conclusions: The present study suggests that the exposed radiology personnel should carefully comply with radiation protection procedures such as wearing of lead apron during diagnostic proce- dures and minimize radiation exposure where possible

Comet Assay

Comet tail

Source

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GC-MS Profiling and Biomedical Applications of Essential Oil of Euphorbia larica Boiss.: A New Report

Antioxidants (2023)

Muddaser Shah Faizullah Khan Saeed Ullah Tapan Kumar Mohanta Ajmal Khan

The present study explored Euphorbia larica essential oil (ELEO) constituents for the first time, obtained via hydro-distillation by means of Gas Chromatography-Mass Spectrometry (GC-MS) profiling. The essential oil was screened in vitro against breast cancer cells, normal cell lines, α-glucosidase, carbonic anhydrase-II (CA-II), free radical scavenging and in vivo analgesic and anti-inflammatory capabilities. The GC-MS screening revealed that the ELEO comprises sixty compounds (95.25%) with the dominant constituents being camphene (16.41%), thunbergol (15.33%), limonene (4.29%), eremophilene (3.77%), and β-eudesmol (3.51%). A promising antidiabetic capacity was noticed with an IC50 of 9.63 ± 0.22 μg/mL by the ELEO as equated to acarbose with an IC50 = 377.71 ± 1.34 μg/mL, while a 162.82 ± 1.24 μg/mL inhibition was observed against CA-II. Regarding breast cancer, the ELEO offered considerable cytotoxic capabilities against the triple-negative breast cancer (MDA-MB-231) cell lines, having an IC50 = 183.8 ± 1.6 μg/mL. Furthermore, the ELEO was also tested with the human breast epithelial (MCF-10A) cell line, and the findings also presumed that the ELEO did not produce any damage to the tested normal cell lines. The ELEO was effective against the Gram-positive bacteria and offered a 19.8 ± 0.02 mm zone of inhibition (ZOI) against B. atrophaeus. At the same time, the maximum resistance with 18.03 ± 0.01 mm ZOI against the fungal strain Aspergillus parasiticus was observed among the tested fungal strains. An appreciable free radical significance was observed via the DPPH assay with an IC50 = 133.53 ± 0.19 µg/mL as equated to the ABTS assay having an IC50 = 154.93 ± 0.17 µg/mL. The ELEO also offered a substantial analgesic capacity and produced 58.33% inhibition in comparison with aspirin, a 68.47% decrease in writhes, and an anti-inflammatory capability of 65.54% inhibition, as equated to the standard diclofenac sodium having 73.64% inhibition. Hence, it was concluded that the ELEO might be a natural source for the treatment of diabetes mellitus, breast cancer, analgesic, inflammatory, and antimicrobial-related diseases. Moreover, additional phytochemical and pharmacological studies are needed to isolate responsible chemical ingredients to formulate new drugs for the examined activities.

10.3390/antiox12030662

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Citations (13)

Corrigendum: Bioassay-Guided Isolation of Sesquiterpene Coumarins from Ferula narthex Bioss: A New Anticancer Agent

Frontiers in Pharmacology (2016)

Mahboob Alam Ajmal Khan Abdul Wadood Ayesha Khan Shumaila Bashir

[This corrects the article on p. 26 in vol. 7, PMID: 26909039.].

10.3389/fphar.2016.00444

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Genome Subtraction and Comparison for the Identification of Novel Drug Targets against Mycobacterium avium subsp. hominissuis

Pathogens (2020)

Reaz Uddin Bushra Siraj Muhammad Rashid Ajmal Khan Sobia Ahsan Halim

Mycobacterium avium complex (MAC) is a major cause of non-tuberculous pulmonary and disseminated diseases worldwide, inducing bronchiectasis, and affects HIV and immunocompromised patients. In MAC, Mycobacterium avium subsp. hominissuis is a pathogen that infects humans and mammals, and that is why it is a focus of this study. It is crucial to find essential drug targets to eradicate the infections caused by these virulent microorganisms. The application of bioinformatics and proteomics has made a significant impact on discovering unique drug targets against the deadly pathogens. One successful bioinformatics methodology is the use of in silico subtractive genomics. In this study, the aim was to identify the unique, non-host and essential protein-based drug targets of Mycobacterium avium subsp. hominissuis via in silico a subtractive genomics approach. Therefore, an in silico subtractive genomics approach was applied in which complete proteome is subtracted systematically to shortlist potential drug targets. For this, the complete dataset of proteins of Mycobacterium avium subsp. hominissuis was retrieved. The applied subtractive genomics method, which involves the homology search between the host and the pathogen to subtract the non-druggable proteins, resulted in the identification of a few prioritized potential drug targets against the three strains of M. avium subsp. Hominissuis, i.e., MAH-TH135, OCU466 and A5. In conclusion, the current study resulted in the prioritization of vital drug targets, which opens future avenues to perform structural as well as biochemical studies on predicted drug targets against M. avium subsp. hominissuis.

Druggability

Proteome

Suppression subtractive hybridization

Functional Genomics

10.3390/pathogens9050368

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Citations (17)