Dominika Szkopek

Kielanowski Institute of Animal Physiology and Nutrition

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Paweł Konieczka

University of Warmia and Mazury in Olsztyn

Jarosław Woliński

Polish Academy of Sciences

Misza Kinsner

Kielanowski Institute of Animal Physiology and Nutrition

Piotr Wychowański

Trelleborg (Sweden)

Janine Donaldson

Trelleborg (Sweden)

Kateryna Pierzynowska

Kielanowski Institute of Animal Physiology and Nutrition

Katarzyna Roszkowicz-Ostrowska

Kielanowski Institute of Animal Physiology and Nutrition

Kamil Zaworski

Kielanowski Institute of Animal Physiology and Nutrition

Stefan Pierzynowski

Lund University

Michał Wszoła

Wilmington University

Cooperative Institutions

Polish Academy of Sciences

Warsaw University of Life Sciences

Kielanowski Institute of Animal Physiology and Nutrition

University of Warmia and Mazury in Olsztyn

Medical University of Warsaw

Institute of Plant Genetics, Polish Academy of Sciences

University of Life Sciences in Poznań

University of Life Sciences in Lublin

Wilmington University

University of Computer Sciences and Economics in Olsztyn

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90-OR: 3D Bioprinting of a Bionic Pancreas with a Vascular System—Results of Transplantation in Large Animals

Diabetes (2023)

Michał Wszoła Andrzej Berman Marta Klak Tomasz Dobrzański OLIWIA JANOWSKA

The transplantation of the pancreas is recommended in diabetes with complications. The combination of cell biology and 3D-bioprinting can create organs with vasculature which should be functional. There are issues to be solved: leakage, thrombosis, enhancement against high pressure, connecting organ to the recipient. The purpose of this study was to demonstrate 3D-bioprinting of bionic organs with use of dECM-derived biomaterials with stable flow through the organ in vitro and in large animal studies. Materials and methods: Mathematical analysis in the designed bionic pancreas was performed. The bionic pancreas was 3D bioprinted with 2 types of bioinks. The first tests were carried out in a bioreactor then organs were subjected to magnetic resonance and X-ray with contrast. Pressure endurance tests were performed. 14 pigs received 3D-bioprinted bionic pancreas transplantation. DCL-VESS-Group (n=5) received decellularized vessels as anastomosis. 9 pigs received bionic pancreas with vascular prosthesis as anastomosis - Prosthesis-Group. Pigs were observed up to 14 days. Hist-pat analysis of removed pancreas was performed. Results: The tests carried out in the bioreactor showed the stability of the bionic organ and vascular system in MRI and X-ray examination for 5 days. No leaks beyond the bionic pancreas were observed up to 400 mmHg. In all 14 cases of transplantation there was stable flow throughout the organ after release of vascular clamp. DCL-VESS-Group - In all cases vascular cloth was observed. Prosthesis-Group - In 5 cases there were no complications influencing blood flow through the organ. 4 died to postoperative complications. Stable flow within a transplanted bionic organ was observed till 2 weeks post - surgery within ultrasound examination and radiology intervention. Histopatological showed the presence of CD31 / PECAM-1 (new blood formation). Conclusion: It is feasible to 3Dbioprint and transplant bionic organ in big animal model. Disclosure M.Wszola: Board Member; Polbionica Ltd., Stock/Shareholder; Polbionica Ltd.. K.I.Roszkowicz-ostrowska: None. L.Kownacki: None. J.Wolinski: None. A.Dobrzyn: None. A.Kaminski: None. A.Berman: Board Member; Polbionica Ltd., Stock/Shareholder; Polbionica Ltd. M.Klak: Employee; Polbionica Sp. z o.o., Stock/Shareholder; Polbionica Sp. z o.o. T.Dobrzanski: Employee; Polbionica. O.Janowska: Employee; POLBIONICA Sp. z o.o. S.Domanski: Employee; Polbionica. D.Piatek: None. J.Wejman: None. D.Szkopek: None.

Decellularization

Pancreas transplantation

10.2337/db23-90-or

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Bacillus-based probiotics affect gut barrier integrity in different ways in chickens subjected to optimal or challenge conditions

Veterinary Microbiology (2021)

Paweł Konieczka Dorthe Sandvang Misza Kinsner Dominika Szkopek Natalia Szyryńska

Bacillus licheniformis

Strain (injury)

Clostridium perfringens

Cecum

Prebiotic

10.1016/j.vetmic.2021.109323

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Citations (14)

Changes in lipids metabolism indices as a result of different form of selenium supplementation in chickens

Scientific Reports (2022)

Damian Bień M. Michalczuk Dominika Szkopek Misza Kinsner Paweł Konieczka

Abstract Selenium is an essential element that is important for many metabolic processes. Feed components used in chicken nutrition, especially cereals, may be deficient in selenium, hence selenium supplementation is necessary. Taking into account the progress in breeding, and thus the higher demand of birds for this element, it seems obvious to investigate an increased selenium dose in the diet of chickens. The aim of the study was to evaluate the effect of feed enriched with different forms of selenium at an increased dose of 0.5 mg/kg feed on the profile and metabolism of fatty acids in the breast muscle and liver of chickens. The study was conducted on 300 Ross 308 chickens reared for 42 days under standard conditions. The control group received feed supplemented with sodium selenite at a dose of 0.3 mg/kg feed. The research groups received different forms of selenium (sodium selenate, selenised yeast, nano-selenium) at an increased dose of 0.5 mg/kg feed. The study showed that the administration of different forms of selenium in the feed affected its concentration in the breast muscle and liver ( p ≤ 0.01). Nano-selenium was found to have a high bioavailability, but also a lower risk of toxicity compared to other forms of selenium. Using different forms of selenium ( p ≤ 0.01) at a dose of 0.5 mg/kg feed can significantly modify the fatty acid profile, lipid and enzymatic indices of fatty acid metabolism in breast muscle and liver.

Feed additive

Sodium selenate

Selenate

Animal Feed

10.1038/s41598-022-18101-2

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The Regulatory Role of Pancreatic Enzymes in the Maintenance of Small Intestinal Structure and Enterocyte Turnover with Special Reference to Alpha Amylase

International Journal of Molecular Sciences (2024)

Kamil Zaworski Piotr Wychowański Dominika Szkopek Jarosław Woliński Janine Donaldson

The aim of this study was to elucidate the impact of porcine pancreatic enzymes (Creon® pancrelipase) in comparison to microbial-derived alpha amylase (MD amylase) on the small intestine wall structure, mucosal glycogen accumulation, and enterocyte turnover. The impact of enzyme supplementation on the small intestine was explored in 18 pigs with surgically induced exocrine pancreatic insufficiency (EPI). Four healthy pigs served as the control group. EPI led to reduced villus length, crypt depth, and thickness of the mucosa and muscularis layers compared to those of healthy pigs. All these changes appeared to be reversible after enzyme supplementation. Brush border thickness was decreased in EPI and increased with both enzyme preparations, with MD amylase treatment leading to the highest values in the proximal jejunum. No EPI-induced changes were observed in the goblet cell (GC) population, but significant increases in GC number and area were observed following MD amylase treatment. Glycogen accumulation within the duodenal mucosa was significantly increased in EPI pigs. EPI was also shown to significantly increase apoptotic activity and decrease proliferative activity in comparison to healthy animals, while both enzyme preparations resulted in the complete recovery of both proliferative and apoptotic activity in all investigated intestinal segments. Creon® influenced the morphology of the small intestine. However, supplementation of exogenous microbial amylase alone also affected gut morphology in a similar way to that of the complex host pancreatic enzymes offered orally. These data indicate that in addition to their role in digestion of nutrients in EPI, intraluminal pancreatic enzymes, especially amylase, contribute to gut health through maintenance of the intestinal wall architecture and physiological enterocyte turnover.

Enterocyte

Jejunum

Digestive enzyme

Exocrine pancreatic insufficiency

Brush border

Mucin 2

10.3390/ijms26010249

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A review: Pancreatic enzymes in the treatment of chronic pancreatic insufficiency in companion animals

Journal of Veterinary Internal Medicine (2024)

Dominika Szkopek Stefan Pierzynowski Kateryna Pierzynowska Kamil Zaworski Agata Kondej

Abstract The purpose of this review was to analyze the scientific literature on exocrine pancreatic insufficiency (EPI) in dogs and cats and our own research on porcine model to compare animal‐ and microbial‐derived enzymes in the treatment of animals with this disease. Clinical signs of EPI occur when more than 85% of the pancreatic parenchyma is non‐functional. EPI can be a consequence of various diseases. The insufficient activity or deficiency of pancreatic enzymes leads to impaired digestion and absorption, and consequently, to malnutrition. The primary treatment for enzyme insufficiency is pancreatic enzyme replacement therapy (PERT). PERT in animals with EPI is a lifetime therapy. Most commercially available products are of animal origin (processed pancreata obtained from a slaughter house) and contain lipases, alpha‐amylase, and proteases. Enzymes of microbial and plant origin seem to be a promising alternative to animal‐derived enzymes, but to date there are no registered preparations containing all enzymes simultaneously for use in clinical practice to treat EPI. Results from some previous studies have highlighted the “extra‐digestive” functions of pancreatic enzymes, as well as the actions of pancreatic‐like microbial enzymes. For example, trypsin activates protease‐activated receptor and provokes maturation of enterocytes and enterostatin inhibits fat absorption. It has been postulated that intrapancreatic amylase is the main component of the acini‐islet‐acinar axis—the reflex which down regulates insulin release, while gut and blood amylase exhibit anti‐incretin actions “per se.” Additionally, high but still physiological blood amylase activity coincide with physiological glucose homeostasis and a lack of obesity.

Pancreatic enzymes

Exocrine pancreatic insufficiency

10.1111/jvim.17096

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Citations (5)

Interaction Between Peroxisome Proliferator-Activated Receptors and Cannabidiol in the Gut of Chickens Applied to Different Challenge Conditions

International Journal of Molecular Sciences (2024)

Dominika Szkopek Marta Mendel Misza Kinsner Bartosz Fotschki Jerzy Juśkiewicz

Peroxisome proliferator-activated receptors (PPARs) are important targets for cannabidiol (CBD), which mediate many of its biological actions. The hypothesis of the present research assumed that PPARs affect the gut response to different challenge factors in chickens (

10.3390/ijms252111398

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P144. Feeding pregnant sows and their offspring Bacillus-based probiotic is an efficient way to improve gut function in piglets at weaning

Animal - science proceedings (2022)

R. Zabielski Paweł Konieczka J. N. Jørgensen J Olszewski Magdalena Mazur-Kuśnierek

10.1016/j.anscip.2022.03.347

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Gradually Increasing Vitamin E Dose Allows Increasing Dietary Polyunsaturation Level While Maintaining the Oxidation Status of Lipids and Proteins in Chicken Breast Muscle

Annals of Animal Science (2021)

Piotr Sałek Paweł Konieczka Wiesław Przybylski Danuta Jaworska Aleksandra Rosikiewicz

Abstract Feeding broilers diets high in n-3 long-chain polyunsaturated fatty acids (n-3 PUFA) increases their incorporation into the meat but it may compromise meat quality due to oxidation of lipids and protein. Increased dietary vitamin E (vE) level downregulates this process, but its excessive level might exceed the physiological requirements for the maintenance of redox balance. This study investigated the sensory characteristics and oxidative status of meat from chickens fed diets supplemented with fish oil (FO) with or without gradually increasing doses of vE. The meat samples were obtained from a total of 27 female broilers of Ross 308 strain (9 birds per each of 3 dietary treatments), which were housed according to the standard management practice for commercial chicken houses over a period of 36 days. Chickens were fed diets containing 80 g/kg of supplemental fat, but the diets differed in fat composition; control diet (80 g/kg diet beef tallow as supplemental fat and a basal vE dose); 40 IU of dl-α-tocopheryl acetate; diet containing mixture of FO and beef tallow as supplemental fat (50:30 w/w g/kg diet) and a basal vE dose (E1), or diet (E2) as diet E1 but with gradually increasing vE dose (120/240 IU/kg diet fed between days 8–21 and days 22–36, respectively). The highest sensory quality and the lowest oxidative status of meat was found in the control chickens. FO decreased the sensory quality of the meat and increased lipid oxidation as well it had an impact on the lipid profile in muscle tissue (PUFA, n-3 ALA, EPA, DHA). Administration of a graded vE dose increased the sensory quality of the meat and did not limit lipid oxidation but maintained protein oxidation balance.

Tallow

Lipid Oxidation

10.2478/aoas-2021-0017

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Cannabis-derived cannabidiol and nanoselenium improve gut barrier function and affect bacterial enzyme activity in chickens subjected to C. perfringens challenge

Veterinary Research (2020)

Paweł Konieczka Dominika Szkopek Misza Kinsner Bartosz Fotschki Jerzy Juśkiewicz

Abstract Revealing the multifocal mechanisms affecting cross-talk between Clostridium perfringens pathogenesis and the host response is an urgent need in the poultry industry. Herein, the activity of Cannabis sativa -derived cannabidiol (CBD) and selenium nanoparticles (Nano-Se) in modulating the host response to Clostridium perfringens challenge was investigated in broiler chickens subjected to a mild infection model. The infected chickens exhibited no clinical manifestations, confirming the potential hazard of pathogen transmission to the food chain in the commercial sector. However, both CBD and Nano-Se affected the responses of chickens to C. perfringens challenge. The beneficial actions of both agents were manifested in the upregulated expression of genes determining gut barrier function. Both CBD and Nano-Se promoted shifts in gut bacterial enzyme activity to increased energy uptake in challenged chickens and upregulated potential collagenase activity. There was no opposite effect of CBD and Nano-Se in mediating the host response to challenge, whereas an additive effect was evidenced on the upregulation of gene determining gut integrity. Collectively, these findings indicate that understanding the action mechanisms of CBD and Nano-Se is of great interest for developing a preventive strategy for C. perfringens infection in broilers.

Clostridium perfringens

10.1186/s13567-020-00863-0

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Citations (31)

Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs

Nutrients (2022)

Monika Słupecka‐Ziemilska Stefan Pierzynowski Paulina Szczurek Kateryna Pierzynowska Piotr Wychowański

Butyrate, a by-product of gut bacteria fermentation as well as the digestion of fat in mother's milk, exerts a wide spectrum of beneficial effects in the gastrointestinal tissues. The present study aimed to determine the effects of sodium butyrate on small intestine contractility in neonatal piglets. Piglets were fed milk formula alone (group C) or milk formula supplemented with sodium butyrate (group B). After a 7-day treatment period, isometric recordings of whole-thickness segments of the duodenum and middle jejunum were obtained by electric field stimulation under the influence of increasing doses of Ach (acetylocholine) in the presence of TTX (tetrodotoxin) and atropine. Moreover, structural properties of the intestinal wall were assessed, together with the expression of cholinergic and muscarinic receptors (M1 and M2). In both intestinal segments (duodenum and middle jejunum), EFS (electric field stimulation) impulses resulted in increased contractility and amplitude of contractions in group B compared to group C. Additionally, exposure to dietary butyrate led to a significant increase in tunica muscularis thickness in the duodenum, while mitotic and apoptotic indices were increased in the middle jejunum. The expression of M1 and M2 receptors in the middle jejunum was significantly higher after butyrate treatment. The results indicate increased cholinergic signaling and small intestinal growth and renewal in response to feeding with milk formula enriched with sodium butyrate in neonatal piglets.

Jejunum

Sodium butyrate

Contractility

10.3390/nu14204301

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Citations (1)