Iman Jamhiri

Shiraz University of Medical Sciences

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Davood Mehrabani

Stem Cell Technology Research Center

Shahrokh Zare

Shiraz University of Medical Sciences

Zahra Khodabandeh

Shiraz University of Medical Sciences

Mehdi Dianatpour

Shiraz University of Medical Sciences

Anahid Safari

Shiraz University of Medical Sciences

Amin Derakhshanfar

Shiraz University of Medical Sciences

Javad Moayedi

Shiraz University of Medical Sciences

Farzaneh Foroughinia

Monash Health

Nader Tanideh

Shiraz University of Medical Sciences

Parisa Dolati

Shiraz University

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Shiraz University of Medical Sciences

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Shiraz University

Stem Cell Technology Research Center

Namazi Hospital

Tehran University of Medical Sciences

Shahid Beheshti University of Medical Sciences

Islamic Azad University, Science and Research Branch

West Kazakhstan Marat Ospanov State Medical University

Bushehr University of Medical Sciences

UNSW Sydney

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The pattern of IL-24/mda-7 and its cognate receptors expression following activation of human hepatic stellate cells

Biomedical Reports (2017)

Iman Jamhiri Seyed Younes Hosseini Davood Mehrabani Zahra Khodabandeh Ramin Yaghobi

Activation of hepatic stellate cells (HSCs) is the pivotal event during liver fibrosis. Interleukin (IL)‑24/melanoma differentiation‑associated gene‑7 (mda‑7) has attracted attention in the pathophysiology of some diseases, while its role in activation/suppression of human HSCs is still unclear. It is important to elucidate whether the expression levels of the IL‑24/mda‑7 protein and its receptors in HSC cells are changed following activation. LX‑2 cells, a human hepatic stellate cell line were activated by a combination of leptin and serum starvation. The activation state was evaluated through measuring the mRNA expression of profibrotic molecules, collagen‑I, TIMP metalloproteinase inhibitor‑1 and transforming growth factor‑β. The expression of IL‑24/mda‑7 was assessed in mRNA and protein levels by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and ELISA methods, respectively. Hence, the amount of IL‑22R1 and IL‑20R2 subunit expression was also compared in activated and normal LX‑2 cells by RT‑qPCR. The expression level of IL‑24/mda‑7 and its cognate receptors was detectable both in the normal and activated LX‑2 cell line. Furthermore, in activated LX‑2, a significant increase of IL24 expression either on IL‑22R1 and IL‑20R2 subunits was also noticeable in comparison to normal cells. The activation state of LX‑2 cells caused significant changes of IL‑24/mda‑7 and its receptors expression. In addition, the elevation in IL‑24/mda‑7 during LX‑2 cell activation, suggested that IL‑24/mda‑7 and its cognate receptors serve a possible role in the development of the fibrosis process. Therefore, IL‑24/mda‑7 and relevant signaling pathways may be employed as a target for fibrosis treatment.

Hepatic stellate cell

10.3892/br.2017.931

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Citations (9)

Tracking healing effect of human Wharton’s jelly stem cells labeled with superparamagnetic iron oxide nanoparticles seeded onto poly vinyl alcohol/chitosan/carbon nanotubes in burn wounds by MRI, and Prussian Blue staining

Biomedical Materials (2024)

Seyedeh‐Sara Hashemi Zeinab Niknam Seyed Mojtaba Zebarjad Davood Mehrabani Reza Jalli

Regenerative medicine by applying tissue engineering and cell transplantation has provided a new door in wound healing. This study tracked healing effect of human Wharton's jelly stem cells (WJSCs) labeled with superparamagnetic iron oxide nanoparticles (SPIONs) seeded onto poly vinyl alcohol/chitosan/carbon nanotubes (PVA/CS/CNT) in burn wounds by magnetic resonance imaging (MRI) and Prussian Blue staining. .

Vinyl alcohol

Prussian blue

Regenerative Medicine

Polyvinyl Alcohol

Superparamagnetism

Iron oxide nanoparticles

10.1088/1748-605x/ad9fc6

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Lipophilic tracer Dil and fluorescence labeling of acridine orange used for Leishmania major tracing in the fibroblast cells

Heliyon (2019)

Narjes Yektaeian Davood Mehrabani Mozhdeh Sepaskhah Shahrokh Zare Iman Jamhiri

BackgroundThis study aims to evaluate the use of fluorescent dye Dil and super vital dye acridine orange (AO) in vitro tracking of labeled L. major in the fibroblast cells.MethodsDil crystal and AO were used to stain L. major in a co-culture of the fibroblasts with the parasite. AO staining solution was added to 1 × 106 parasites. After 10 min, the stained parasites were centrifuged and washed seven times with phosphate buffered saline (PBS). The stained promastigote was incubated with fibroblasts for 6–8 h. The presence of stained parasites with AO in the fibroblast was assessed using a fluorescence microscope. 1 × 106/mL promastigote of L. major was gently suspended and mixed by Dil staining solution with an ultimate concentration of 0.002 μg/mL and it was kept for 20 min at the room temperature. Subsequently, after washing it in PBS for seven times, it was centrifuged at 3000 rpm for 10 min. The supernatant was removed and the precipitate containing stained promastigote was suspended in fresh DMEM F12 with fibroblasts at 37 °C for 6 h. The presence of stained parasites with Dil in fibroblast was assessed using a fluorescence microscope. Fibroblast characterization was undertaken by a real-time polymerase chain reaction (PCR).ResultsAcridine orange staining assisted in detection of the live parasite in the fibroblast cells. Free promastigote looked green before entering into the fibroblasts after 12 h culture. The parasite entered the cytoplasm of fibroblasts at the beginning of the exposure and gradually entered the nucleus of the fibroblast. The fibroblast nucleus was entirely stained green by AO. The L. major appeared green under the fluorescent microscope. Dil staining revealed that the internalized parasites with red/orange color were localized within the cytoplasm after 6-hours and the nucleus of the fibroblasts after 72-hours following culture. Human fibroblasts were positive at the expression of CD10, CD26, matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-3 (MMP-3) and negative for CD106 and integrin alpha 11.ConclusionThe fluorescent dye Dil staining is a safe, easy to use, inexpensive and fast method for labeling of the Leishmania parasite in the fibroblast cells. Acridine orange staining could be useful for tracing the parasites in the fibroblasts too. In this study, both Dil and AO were compared and considered as suitable vital dyes for identifying labeled Leishmania in the fibroblast in vitro, but Dil was superior to AO with its feature does not transfer from the labeled to unlabeled cells.

Acridine orange

Dermal fibroblast

10.1016/j.heliyon.2019.e03073

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Citations (23)

in Vitro Effect of Methamphetamine on Proliferation, Differentiation and Apoptosis of Adipose Tissue Stem Cells

Journal of Pharmacy & Pharmaceutical Sciences (2022)

Leyla Anari Davood Mehrabani Mahboobeh Nasiri Shahrokh Zare Iman Jamhiri

Among abused substances, methamphetamine is a psychostimulant drug widely used recreationally with public health importance. This study investigated the effect of methamphetamine on proliferation, differentiation, and apoptosis of human adipose tissue stem cells (AdSCs).AdSCs were isolated from human abdominal adipose tissue and were characterized for mesenchymal properties and growth kinetics. MTT assay was undertaken to assess methamphetamine toxicity on proliferation and differentiation properties and apoptosis of hAdSCs.Isolated cells were shown to have mesenchymal properties and a population doubling time (PDT) of 40.1 h. Following methamphetamine treatment, expressions of KI-67 and TPX2 as proliferation genes and Col1A1 and PPARg as differentiation genes decreased. Methamphetamine administration increased the expression of Bax and decreased Bcl-2 genes responsible for apoptosis.Our data suggested when AdSCs were exposed to methamphetamine, it decreased proliferation and differentiation properties of stem cells together with an increase in apoptosis. These findings can be added to the literature, especially when methamphetamine is used recreationally for weight loss purposes.

Methamphetamine

10.18433/jpps31843

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Response to Mungmunpuntipantip and Wiwanitkit

Journal of Clinical Neuroscience (2022)

Farzaneh Foroughinia Najmeh Morovati Anahid Safari Mehdi Dianatpour Iman Jamhiri

10.1016/j.jocn.2022.03.041

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Effect of high intensity interval training and detraining on gene expression of AKT/FoxO3a in cardiac and soleus muscle of male rats

EBNESINA (2020)

Shahin Sheibani Farhad Daryanoosh Nader Tanideh Mostafa Rahimi Iman Jamhiri

High-Intensity Interval Training

Interval training

Cardiac muscle

10.22034/22.2.15

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The effect of docetaxel on survival, fertilization rate and apoptosis-related genes mRNA expression in mouse metaphase II oocytes following vitrification.

PubMed (2021)

Zahra Khodabandeh Iman Jamhiri Naeimeh Dehghani Hamed Daneshpazhouh Bahia Namavar Jahromi

Docetaxel is beneficial in oocyte cryopreservation.The effect of docetaxel, on the survival, fertilization rate and mRNA expression of apoptosis-related genes of vitrified mature oocytes was investigated.Mature oocytes were divided into eight experimental groups, including I) control, II) docetaxel, III) docetaxel + cryoprotectant agent 1 (CPA1), IV) docetaxel + CPA2, V) docetaxel + vitrification 1 (Vit1), VI) docetaxel + Vit2, VII) Vit1, and VIII) Vit2. The survival and fertilization rates, and the mRNA expression level of Bcl-xl, Bax and caspase-3 as apoptosis-related genes were evaluated.The survival rates in Vit1, and Vit2 groups were significantly lower than in the control group (P<0.05). The fertilization rates in docetaxel + Vit1, docetaxel + Vit2, Vit1, and Vit2 were significantly lower than the control, docetaxel, and related groups using docetaxel and CPAs. Bax expression was significantly increased in groups which oocytes vitrified. Also, its expression in the Vit2 group increased significantly in comparison to the docetaxel + Vit2 group. The expression of the Bcl-xl gene was downregulated in docetaxel + CPA2, docetaxel + Vit2 and Vit2 compared to docetaxel group. The Bax/Bcl-xl ratio significantly increased in docetaxel + CPA2, docetaxel + Vit1, docetaxel + Vit2, Vit1 and Vit2 groups compared to control, docetaxel and the docetaxel + CPA1 group. Caspase-3 expression significantly increased in all six groups in comparison to the control, and docetaxel groups. Its expression significantly increased in the Vit1 and Vit2 groups in comparison with docetaxel + Vit1, and docetaxel + Vit2, respectively.Docetaxel ameliorates the damages to oocytes during vitrification by altering the expression of apoptosis-related genes and its effects are dependent on the vitrification solution used in cryopreservation of oocytes.

Vitrification

10.22099/ijvr.2020.38128.5554

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Impact of 8-week cold-and warm water swimming training combined with cinnamon consumption on serum METRNL, HDAC5, and insulin resistance levels in diabetic male rats

Heliyon (2024)

Seyed Morteza Tayebi Saleh Motaghinasab Rasoul Eslami Somayeh Ahmadabadi Aref Basereh

ObjectiveNumerous studies have reported the beneficial effects of exercise and the use of herbal supplements in improving type 2 diabetes and insulin resistance. However, there are still many unanswered questions about the effects of cold and hot water, exercise, and herbal supplements on meteorine-like protein (METRNL), which is considered one of the key factors influencing insulin resistance improvement in this condition. Hence, the current study aimed to address these knowledge gaps and investigate the effects of 8 weeks of warm and cold-water swimming exercise with cinnamon consumption on serum levels of METRNL, histone deacetylase-5 (HDAC5), and insulin resistance in diabetic male rats.MethodsFor this purpose, 70 diabetic male rats were randomly divided into seven groups (10 rats in each group) (1Saeidi A. Hackney A.C. Tayebi S.M. Ahmadian M. Zouhal H. Diabetes, Insulin Resistance, Fetuin-B and Exercise Training.Annals of Applied Sport Science. 2019; 7: 1-2Crossref Scopus (8) Google Scholar) Healthy control (HC), (2Tayebi S.M. Ghanbari-Niaki A. Saeidi A. Hackney A.C. Exercise Training, Neuregulin 4 and Obesity.Ann Appl Sport Sci. 2017; 5: 1-2Crossref Scopus (9) Google Scholar) Diabetic control, (3Saeidi A. Tayebi S.M. Khosravi A. Razi O. Sellami M. Abderrahman A.B. et al.Obesity, Fat Mass, Osteopontin and Exercise Training.International Journal of Applied Exercise Physiology. 2019; 8: 177-179Google Scholar) swimming training in cold water (temperature 5 °C), (4Rao Kondapally Seshasai S. Kaptoge S. Thompson A. Di Angelantonio E. Gao P. Sarwar N. et al.Diabetes mellitus, fasting glucose, and risk of cause-specific death.The New England journal of medicine. 2011; 364: 829-841Crossref PubMed Scopus (0) Google Scholar) swimming training at 5‌‌ °C + cinnamon consumption (200 mg/kg body weight), (5Petersmann A. Müller-Wieland D. Müller U.A. Landgraf R. Nauck M. Freckmann G. et al.Definition, classification and diagnosis of diabetes mellitus.Experimental and Clinical Endocrinology & Diabetes. 2019; 127: S1-S7Crossref PubMed Scopus (0) Google Scholar) swimming training in warm water (temperature 36-35 °C), (6Kolahdouzi S. Baghadam M. Kani-Golzar F.A. Saeidi A. Jabbour G. Ayadi A. et al.Progressive circuit resistance training improves inflammatory biomarkers and insulin resistance in obese men.Physiology & behavior. 2019; 205: 15-21Crossref Scopus (43) Google Scholar) swimming training in warm water (temperature 36-35 °C) + consumption of cinnamon, and (7Dezhkam N. Rezaeian N. Effect of Six Weeks of Aerobic Training on Meteorin Like Factor Response and Insulin Resistance Index in Overweight and Obese Young Women.Journal of Applied Health Studies in Sport Physiology. 2021; 8: 28-35Google Scholar) consumption of cinnamon only.ResultsThe present study revealed a significant increase in serum METRNL concentration in the cold-water swimming + cinnamon consumption group (p<0.05). However, no significant changes were observed in insulin levels and HOMA-IR across the different groups (p>0.05). Additionally, noteworthy findings included a significant reduction in HDAC5 levels in both the cold-water swimming group and the cold-water swimming + cinnamon consumption group, as well as a significant decrease in fasting blood sugar (FBS) levels in all groups compared to the HC group (p<0.05).ConclusionsThe results of the present study demonstrate that the combination of cold-water swimming exercises and cinnamon extract consumption led to notable increases in serum METRNL concentration. Additionally, significant reductions were observed in HDAC5 and FBS levels. These findings highlight the potential effectiveness and benefits of the combination of cold-water swimming exercises and cinnamon extract consumption as an approach to improve diabetes-related indices.

Water consumption

10.1016/j.heliyon.2024.e29742

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Citations (2)

Mutagenesis in DYRK1B by Overlap Extension-PCR (OE-PCR) Technique and Synthesis of Recombinant Lentivirus Carrying DYRKB R102C

SSRN Electronic Journal (2022)

Afrooz Daneshparvar Vahid Razban Iman Jamhiri Jafar Fallahi behnam moghtaderi

Missense mutations in the DYRK1B gene have been found related to metabolic syndrome. The substitution of arginine to cysteine at position 102 (R102C) is in the extremely conserved domain of kinase-like and changes this protein function. our aim is produce of DYRK1B R102C mutation using Overlap Extension-PCR strategy and cloning in the lentiviral vector as a long term strategy of the gene expression modulation. The RNA extraction was performed from fat tissue of mouse and cDNA was synthesized. Forward and reverse mutagenic primers were designed based on mutation site and overlapping strategy. Three PCR separate reactions were performed to complete the mutagenesis OE-PCR strategy. The mutagenic DNA was cloned in LeGO-iG2 plasmid. The OE-PCR and sequencing results showed that the desired mutation was performed correctly. Finally, we transfected LeGO-iG2, psPAX2 and pMD2 into the HEK293T cells to produce the lentiviruses.

Site-directed mutagenesis

10.2139/ssrn.4131066

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Recombinant NS3 Protein Induced Expression of Immune Modulatory Elements in Hepatic Stellate Cells During Its Fibrotic Activity

Viral Immunology (2018)

Iman Jamhiri Khashayar Shahin Zahra Khodabandeh Kurosh Kalantar Jamal Sarvari

There is a growing body of studies that show the important role of NS3 protein from hepatitis C virus in fibrosis. However, mechanisms of the effects of this protein on immune modulation of stellate cells remain to be investigated. Herein, the effect of NS3 protein on the expression level of suppressor of cytokine signaling (SOCS)1/3 and interleukin-24 (IL-24)-related genes was investigated in hepatic stellate cell (HSC), LX-2. Recombinant NS3 protein was added to LX-2 HSC culture. Leptin and standard medium treatments were also included in experiments as positive and negative controls, respectively. Total RNA was extracted from each well at 6, 12, and 24 h after NS3 addition. The expression levels of the fibrotic (transforming growth factor beta 1 [TGF-β], alpha-smooth muscle actin [α-SMA], and COL1A1), inflammatory (IL-6 and IL-24), IL-20R, IL-22R, and immunosuppressive genes (SOCS1 and SOCS3) were evaluated by real-time polymerase chain reaction (PCR). Recombinant NS3 protein induced activated phenotypes of LX-2 with a significant increase in the expression level of α-SMACOL1A1 (p < 0.0001) and TGF-β. Moreover, this exposure led to a meaningful elevation in the expression of IL-6. Furthermore, compared with leptin (control), after the stellate cell treatment with NS3, SOCS1 and SOCS3 gene expression induced at a comparable level. Compared with the control sample, the NS3 protein significantly increased the expression level of IL-24 and its related receptors, IL-20R and IL-22R. This study not only confirmed the previously proved inflammatory and fibrotic effect of this protein but also indicated that high expression levels of SOCS1, SOCS3, and IL-24 have a significant effect on HSC activation. Therefore, these two molecules can be used as a potential therapeutic target candidate.

Hepatic stellate cell

NS3

Hepatic fibrosis

10.1089/vim.2018.0018

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Citations (3)