Objective:To assess the technical feasibility of sigmoid colon vaginoplasty in patients with Mayer-Rokitansky-Kuster-Hauser(MRKH) syndrome.Methods: 32 patients with MRKH syndrome underwent sigmo-id colon vaginoplasty.The operative technique and the operation complications,the anatomical and functional results of the neovagina were evaluated.Results: The mean operation duration was 156 min(range 130～180 min).The mean fall of haemoglobin was 20 g/L(range 15~33 g/L).The complications included rectal injury(n=1),urinary tract infection(n=1),infection in incision wound(n=1).The mean hospital stay was 10.6 d(range 9~22 d).The mean length of neovagina was 10.7 cm(range 10~12 cm) and the width was about 4 cm.There was an obvious decrease of the discharge and stink of neovagina in the initial 6 months.The patients with regular intercourse achieved satisfaction in half a year or one year after operation.Conclusion: sigmoid colon vaginoplasty is an effective approach MRKH syndrome.
Objective: To isolate and characterize cancer cell subclones with different metastatic potentials from human ovarian cancer cell line 3AO. Methods: By cell clone technique and cell electrophoresis, three sublines (A1, A5 and All) with different metastatic potentials from 3AO were established. The invasiveness and migratory capacities in the three subgroups were observed by vitro growth, Boyden chamber and soft agar cloning. Results: The electrophoretic mobility of A1, A5 and All was (15.30±0.67) μm/s, (11.02±0.60) μm/s and (6.20±0.72) μm/s respectively. The population doubling time was 20.55 h, 28.99 h and 38.48 h respectively. In vitro, A1 was found to be a high invasive cell line in matrigel invasive assay and formed 45 clones in soft agar cloning assay. On the contrary, A11 was found to have low invasive ability and only formed 13 clones. Conclusion: Successful establishment of these subclones with different metastatic potentials may be valuable for further study on the molecular mechanisms of cancer metastasis and cloning of cancer metastasis related genes.
The constant infiltration of Postmodernism thought in our social life has guided the world movie to turn to the Postmodernism way.Films are screen impressions of Postmodernism to challenge the authority and to subvert the tradition.With Films,one of the Ideology delivery platforms,The Postmodernism not only give a subversive reading of traditional and classical things,guess a lot of possibilities of every result,but also reconstruct the meaning of life.The Postmodernism has both positive and negative values,we should analyze it in a dialectical way.
Objective To investigate the effect of intercellular adhesion molecule(ICAM)-1 on invasion of ovarian monoclonal cancer cells.Methods A high invasive subclone(S1) and a low invasive subclone (S21) were separated from human ovarian cancer line SKOV3 using cell clone technique and cell electrophoresis,and inoculated in nude rice.lCAM-I expression in metastatic tissue of nude rice was detected by immunohis- tochemistry. The role of ICAM-I in adhesion and invasion of S1 cells was observed with cell adhesion test and Boyden Chamber invasive assay respectively.Results S1 cell mass showed higher adhesive capacity to surrounding tissue than S21 cell.ICAM-1 expression was significantly stronger in S1 than in S21.Positively stained cells were mainly located at the edge of the tumor .The antibody of ICAM-1 could significantly inhibit S1 cells from adhering to and invading the basement membrane matrix by 41.9% and 55.0% respectively.Conclusion ICAM-I plays an important role in the adhesion and invasion of ovarian cancer.
Insulin-like growth factors (IGFs) can be synthesized and secreted by almost all fetal organs through autocrine and paracrine ways. It can bind to specific receptors on the cell surface to exert its physiological roles in local tissues and strengthen the assimilation of the body. IGFs are necessary for the fetus and play important roles in growth, development and mature of fetal organs. IGFs in maternal plasma and placenta also affect fetal growth and development. Recombinant human IGF-1(rhIGF-1) has been used to treat some obstetric associated diseases.
Objective To observe the effect of antiestrogen and antiprogestin on the proliferation of ovarian epithelial cancer (OEC) cell lines in vitro. Methods A variety of protocols were utilized to conduct this study. Results OEC cell line SKOV3 is ER(+)/PR(-), while OVCAR-3 is ER(+)/PR(+). High dose of tamxifen inhibited the proliferation of both cell lines, while mifepristone only inhibited OVCAR-3. Conclusion Antiestrogen and antiprogestin can inhibit the proliferation of some ovarian epithelial cancer cell lines in vitro.
Objective:To is olate and characterize cancer cell subclones wit h differen t metastatic potential from human ovarian c ancer cell line. Methods:Using cell clone tech nique,cell elect rophoresis and flow cytometer,we establish ed three sublines with different metast atic potential from human ovarian cancer li ne-SKOV3 (S1,S6 and S14).The invasi veness and migratory capacity among the thr ee subgroups were observed by vitro g rowth,Matrigel invasion assay,soft agar cl oning and in viv o tumorigenicity and spontaneous metastasi s assay in nude mice. Results:Among the three subcl ones,S1 expressed as the highly metastatic phenotype,with 70 % metastasis frequency 6 weeks after subcutaneous inoculation into nude mice,wh ereas S14 was not metastatic.In vitr o,S1 was found to be the most highly invasiv e cell line in Matrigel invasive as say and had the most clones in soft agar clo ning assay.While,S1 4 was found to have the least invasive abili ty and the least clones. Conclusions:Successful estab lishment of thes e subclones with different metastatic pote ntial may be valuable for further stud y on the molecular mechanisms of cancer met astasis and cloning of cancer metastasis related g enes. [
Objective: To investigate the expression of intercellular adhesion molecule 1 in ovarian cancer and its role in adhesion and invasion of ovarian cancer cells. Methods: The expression of ICAM-1 in epithelial ovarian neoplasms and subclones of SKOV3 cells was determined by SP immunohistochemical staining and FCM. The role of ICAM-1 in adhesion and invasion of ovarian cancer cells was observed with cell adhesion test and Boyden Chamber invasive assay respectively. Results: The expression of ICAM-1 was 71.8%(28/39)in epithelial ovarian cancer mass and 40%(8/20) in benign mass . The fluorescent intensity of SKOV3-S1, the highly invasive clone of SKOV3, was higher than the noninvasive clone SKOV3-S21. The antibody of ICAM-1 can significantly inhibits the SKOV3-S1 cells adhere to and invade the basement membrane matrix by 41.9% and 55.0% respectively. Conclusions: ICAM-1 plays an important role in the adhesion and invasion of ovarian cancer.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)