Gabriela Rizo

Consejo Nacional de Investigaciones Científicas y Técnicas

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Elina V. García

Universidad Católica de Salta

Mariela Roldán‐Olarte

Instituto Superior de Investigaciones Biológicas

Daniel Barrera

Consejo Nacional de Investigaciones Científicas y Técnicas

Pablo A. Valdecantos

Instituto Superior de Investigaciones Biológicas

Daniela C. García

Consejo Nacional de Investigaciones Científicas y Técnicas

Dora C. Miceli

Instituto Superior de Investigaciones Biológicas

Luis Emanuel Jimenez

Instituto de Química del Noroeste Argentino

D. C. Miceli

Instituto Superior de Investigaciones Biológicas

Rosa M.S. Álvarez

Instituto de Química del Noroeste Argentino

Cooperative Institutions

Consejo Nacional de Investigaciones Científicas y Técnicas

National University of Tucumán

Instituto Superior de Investigaciones Biológicas

Centro Científico Tecnológico - Tucumán

Instituto de Química y Fisicoquímica Biológicas

Universidad Católica de Salta

Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

Instituto de Química del Noroeste Argentino

Escuela Superior Politecnica del Litoral

University of Central Florida

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Exogenous activation and inhibition of plasminogen/plasmin activity during in vitro maturation of bovine cumulus‐oocyte complexes: A biological and spectroscopic approach

Molecular Reproduction and Development (2020)

Gabriela Rizo Daniel Barrera Luis Emanuel Jimenez Elina V. García Daniela C. García

Abstract This study deals with the effect of plasminogen/plasmin on the in vitro maturation (IVM) of bovine cumulus‐oocyte complexes (COCs). Exogenous plasminogen activator streptokinase (SK) added to the IVM medium revealed similar values of cumulus expansion and oocyte nuclear maturation compared to controls (standard IVM medium). However, a decrease in both determinations was observed in COCs matured with the supplementation of ɛ‐aminocaproic acid (ɛ‐ACA), a specific plasmin inhibitor. After in vitro fertilization, no differences were observed in either cleavage or blastocyst rates between SK and control groups; however, ε‐ACA treatment caused a decrease in both developmental rates. Zona pellucida (ZP) digestion time decreased in the SK group while it increased in the ε‐ACA group. Raman microspectroscopy revealed an increase in the intensity of the band corresponding to the glycerol group of sialic acid in the ZP of oocytes matured with SK, whereas ZP spectra of oocytes treated with ɛ‐ACA presented similarities with immature oocytes. The results indicate that although treatment with SK did not alter oocyte developmental competence, it induced modifications in the ZP of oocytes that could modify the folding of glycoproteins. Plasmin inhibition impairs oocyte maturation and has an impact on embryo development, thus evidencing the importance of this protease during IVM.

In vitro maturation

10.1002/mrd.23441

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Citations (2)

Plasminogen activation and plasmin inhibition during in vitro fertilization in bovine: implications for fertilization parameters and early embryo development

Reproductive Biology (2023)

Gabriela Rizo Daniel Barrera Elina V. García Mariela Roldán‐Olarte

Pronucleus

10.1016/j.repbio.2023.100844

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Structural modifications induced by an in vitro maturation process in zona pellucida glycoproteins of bovine oocytes. A Raman microspectroscopy analysis

RSC Advances (2016)

Gabriela Rizo Mariela Roldán‐Olarte D. C. Miceli Luis Emanuel Jimenez Rosa M.S. Álvarez

Raman microspectroscopy is useful for discrimination between immature andin vitromatured bovine oocytes. Modifications in the glycoproteins of the zona pellucida exerted by the maturation methods might influence the process ofin vitroproduction.

Zona pellucida glycoprotein

Raman microspectroscopy

10.1039/c6ra06243a

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Citations (3)

Effect of early addition of bone morphogenetic protein 5 (BMP5) to embryo culture medium on in vitro development and expression of developmentally important genes in bovine preimplantation embryos

Theriogenology (2015)

Elina V. García Dora C. Miceli Gabriela Rizo Pablo A. Valdecantos Daniel Barrera

10.1016/j.theriogenology.2015.04.018

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Expression and localization of urokinase-type plasminogen activator receptor in bovine cumulus–oocyte complexes

Zygote (2015)

Daniela C. García Dora C. Miceli Gabriela Rizo Elina V. García Pablo A. Valdecantos

Summary Urokinase-type plasminogen activator (uPA) is a serine protease involved in extracellular matrix remodeling through plasmin generation. uPA usually binds to its receptor, uPAR, which is anchored to the plasma membrane through a glycosylphosphatidylinositol anchor. uPA/uPAR binding increases proteolytic activity in the neighborhood of the cells containing uPAR and activates intracellular signaling pathways involved in extracellular matrix remodeling, cell migration and proliferation. The aim of this work was to study the expression of uPA, uPAR and plasminogen activator inhibitor-1 (PAI-1) in immature and in vitro matured bovine cumulus–oocyte complexes (COCs). uPA is only expressed in the cumulus cells of immature and in vitro matured COCs, while uPAR and PAI-1 are expressed in both the cumulus cells and the immature and in vitro matured oocytes. In addition, uPAR protein was localized by confocal microscopy in the plasma membrane of oocytes and cumulus cells of immature COCs. Results from this research led us to hypothesize that the uPA/uPAR interaction could cause the local production of uPA-mediated plasmin over oocyte and cumulus cell surface; plasmin formation could also be regulated by PAI-1.

10.1017/s0967199415000076

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Citations (6)