Dina Andriamahavola Rakotondramanana

University of Antananarivo

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Co-Authors

David Ramanitrahasimbola

Institute Malgache De Recherches Appliquées

Nantenaina Tombozara

Institute Malgache De Recherches Appliquées

Zoarilala Rinah Razafindrakoto

Institute Malgache De Recherches Appliquées

Charles Andrianjara

Institute Malgache De Recherches Appliquées

Gabriele Loris Beccaro

University of Turin

Dario Donno

University of Turin

Reine Dorothée Ramilison-Razafimahefa

University of Antananarivo

Marcello Nicoletti

Sapienza University of Rome

Ninà Robertina Nalimanana

Institute Malgache De Recherches Appliquées

Denis Randriamampionona

University of Antananarivo

Cooperative Institutions

Institute Malgache De Recherches Appliquées

Centre National de la Recherche Scientifique

University of Antananarivo

Université de Strasbourg

Istituto Superiore di Sanità

International Centre of Insect Physiology and Ecology

Sapienza University of Rome

Institut Pluridisciplinaire Hubert Curien

University of Turin

CSI Piemonte

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In silico ADMET and anti-inflammatory profiles of the stigmast-4-en-3-one and isolation of isovanillin as the antioxidant principle of Imperata cylindrica (L.) P. Beauv

Natural Product Research (2024)

Zoarilala Rinah Razafindrakoto Nantenaina Tombozara David Ramanitrahasimbola Charles Andrianjara Minjie Zhao

Imperata cylindrica (L.) P. Beauv. is an invasive species widely used in treatment of several diseases associated with pain and inflammation in different countries including Madagascar. This work aims to report the isolation of the antioxidant, analgesic and anti-nflammatory compounds from the methanol extract of I. cylindrica. The bio-guided method was used to isolate its bioactive compounds by combining chromatographic methods, writhing test in mice and antioxidant assays. Stigmast-4-en-3-one was isolated as one among the compounds responsible for the analgesic and anti-inflammatory properties and isovanillin as one among the antioxidant compounds from the extract. Stigmast-4-en-3-one showed a good oral pharmacokinetic profile and good binding affinities with some pro-inflammatory targets. It did not show any mutagenic effect, nor a carcinogenic one and had a low risk to be a cardiotoxic agent. All of our results provide scientific justification for its traditional medicinal use in the management of pain and inflammatory related diseases.

Imperata

Anti-inflammatory

10.1080/14786419.2024.2305649

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Citations (0)

Bronchodilator activity of Phymatodes scolopendria (Burm.) Ching and its bioactive constituent

Journal of Ethnopharmacology (2005)

David Ramanitrahasimbola Dina Andriamahavola Rakotondramanana P. Rasoanaivo Adolphe Randriantsoa Suzanne Ratsimamanga

EC50

10.1016/j.jep.2005.06.037

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Citations (37)

Bio-guided isolation of androsta-1,4-dien-3,16-dione as a vasodilator active principle from the inflorescence of Ravenala madagascariensis Sonn. (Strelitziaceae)

Natural Product Research (2022)

Dina Andriamahavola Rakotondramanana Zoarilala Rinah Razafindrakoto Dario Donno Nantenaina Tombozara Ninà Robertina Nalimanana

Androsta-1,4-dien-3,16-dione was isolated for the first time from the plant kingdom of the ethanolic extract of the Ravenala madagascariensis’ inflorescence by the bio-guided method. Its structure was elucidated by NMR and MS spectroscopic data analysis. The vascular effects of ethanol extracts, fractions and androsta-1,4-dien-3,16-dione were assessed on the phenylephrine pre-contracted isolated rat aorta. The isolated compound exerted the most potent vaso-relaxing effect (EC50 = 109.32 ± 15.82 µM) than the ethanol extract and fractions. The pharmacological mechanism of its vaso-relaxing action was analysed on isolated rat aorta using free-endothelial vascular tissue, specific contracting reagents (CaCl2 and KCl), antagonist (propranolol), enzyme inhibitors (L-NAME, methylene blue) and channel blocker (glibenclamide). Its vaso-relaxing activity could be due, at least partly, to the non-specific inhibition of the calcic influx.

Phenylephrine

Piceatannol

10.1080/14786419.2022.2089668

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Antioxidant, analgesic, anti-inflammatory and antipyretic properties, and toxicity studies of the aerial parts of Imperata cylindrica (L.) Beauv.

South African Journal of Botany (2021)

Zoarilala Rinah Razafindrakoto Nantenaina Tombozara Dario Donno Giovanni Gamba Ninà Robertina Nalimanana

Imperata cylindrica (L.) Beauv. (Poaceae family) is a perennial weed growing in tropical and subtropical areas and it is used in several herbal preparations to treat specific pathologic factors inducing pain. This work aimed to determine the in vitro antioxidant and in vivo anti-inflammatory, analgesic and antipyretic activities of the aerial parts of this plant. The methanol extract (MEIC) was used for (i) phytochemical fingerprint by High-Performance Liquid Chromatography (HPLC), (ii) total phenolic content (TPC) evaluation by the Folin-Ciocalteu colourimetric method, (iii) 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenger spectrophotometric assay, (iv) antioxidant capacity by Ferric Reducing Antioxidant Power (FRAP) assay, (v) analgesic activity evaluated with the acetic acid-induced writhing test, (vi) anti-inflammatory activity evaluation by carrageenan-induced paw oedema, (vii) antipyretic effect on lipopolysaccharide (LPS)-induced fever in rats, and (viii) acute and sub-chronic toxicity in mice. The MEIC presented an average rate of TPC (1920.63 ± 360.62 mgGAE/100 g of dry weight (DW)), which is mostly represented by tannins (37.30%), organic acids (32.84%) and flavonols (10.88%). The DPPH free radical scavenger spectrometric assay showed the antioxidant capacity of the MEIC (IC50 = 192.07 ± 0.78 µg/mL) confirmed by the FRAP assay results (29.60 ± 0.55 mmol Fe2+/Kg of DW). The MEIC decreased significantly the number of writhing in a dose-dependent manner (p < 0.01) showing its analgesic activity; the extract inhibited the inflammation in a dose-dependent manner at the first stage (1st h, p < 0.01) and the second stage (3rd h, p < 0.001) post carrageenan induction and it demonstrated an antipyretic activity by lowering the body temperature (Tb) dose-dependently post LPS induction (i.p. route) and time-dependently at the dose of 200 mg/kg (p < 0.001) in mice. Moreover, the acute and sub-chronic toxicity in mice did not reveal any signs of toxicity and any changes in targeted biochemical and haematological parameters. The quantified phytocompounds could be at the origin of these pharmacological activities. These findings support the beneficial use of this plant to alleviate pains and fever in inflammatory or other diseases. Further studies on the isolation of the bioactive compounds and their action mechanisms should be carried out to confirm these results.

Antipyretic

Imperata

Carrageenan

ABTS

Phytochemical

Anti-inflammatory

10.1016/j.sajb.2021.07.004

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Citations (17)

The first assessment on antioxidant and antidiabetic activities of leaves and stems of Vaccinium secundiflorum Hook. (Ericaceae), an endemic plant of Madagascar

South African Journal of Botany (2020)

Nantenaina Tombozara Dario Donno Zoarilala Rinah Razafindrakoto Denis Randriamampionona David Ramanitrahasimbola

Vaccinium secundiflorum Hook. (Ericaceae family) is an endemic shrub of the Tapia forests of Madagascar widely used by local Malagasy population to treat diabetes mellitus. This study aims to preliminarily determine the antioxidant activity and anti-diabetic properties of this plant. Plant leaf and stem powders were macerated in methanol 95%. The methanol extract (MVS) was used for (i) phytochemical fingerprint by High Performance Liquid Chromatography (HPLC), (ii) total phenolic content (TPC) evaluation by the Folin–Ciocalteu colourimetric method, (iii) 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenger spectrophotometric assay, (iv) antioxidant capacity by Ferric Reducing Antioxidant Power (FRAP) assay, and (v) antidiabetic activity evaluation by oral glucose tolerance test and acute toxicity in mice. Leaves and stems of V. secundiflorum presented a high rate of TPC (4151.02 ± 745.21mgGAE/100 g of dry weight). The HPLC fingerprint analysis showed the presence of several secondary metabolite classes including organic acids (55.31%), phenolic compounds (29.66%), carotenoids (10.90%), vitamin C (3.96%), and monoterpenes (0.10%). The DPPH free radical scavenger spectrometric assay showed a MVS high potential scavenging activity (IC50 = 76.06 ± 1.08 µg/mL). This potential antioxidant capacity was confirmed by the FRAP assay results (69.31 ± 3.31 mmol Fe2+/Kg of dry weight). The oral glucose tolerance test in mice showed a highly significant blood glucose-lowering effect. The acute toxicity in mice revealed that the MVS did not show toxicity at the dose of 2000 mg/kg. Leaves and stems of V. secundiflorum showed significant antioxidant and antidiabetic properties supporting the plant traditional use, even if further studies on bioactive compounds and their action mechanisms should be carried out to confirm these preliminary results.

Phytochemical

Vaccinium

Dry weight

Free radical scavenger

10.1016/j.sajb.2020.01.035

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Citations (12)

Phytochemical and pharmacological activities of Schefflera bojeri (Seem.) R. Vig. (Araliaceae)

South African Journal of Botany (2022)

Nantenaina Tombozara Zoarilala Rinah Razafindrakoto Dario Donno Denis Randriamampionona Reine Dorothée Ramilison-Razafimahefa

Phytochemical

Sapindaceae

10.1016/j.sajb.2022.10.041

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Citations (1)