Frédéric Montfort

École Polytechnique Fédérale de Lausanne

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Christian Depeursinge

King Abdullah University of Science and Technology

Tristan Colomb

Hôpital de Cery

Etienne Cuche

CEA Valduc

Yves Emery

Swiss School of Public Health

Jonas Kühn

University of Bern

Florian Charrière

École Polytechnique Fédérale de Lausanne

Pierre Marquet

University of Lausanne

Nicolas Aspert

École Polytechnique Fédérale de Lausanne

Christophe Moser

École Polytechnique Fédérale de Lausanne

Zahra Monemhaghdoust

École Polytechnique Fédérale de Lausanne

Cooperative Institutions

École Polytechnique Fédérale de Lausanne

University of Lausanne

Hôpital de Cery

University Hospital of Lausanne

University of Liège

King Abdullah University of Science and Technology

Laboratoire d'Informatique Fondamentale de Lille

CEA Valduc

ETH Zurich

Maksymilian Pluta Institute of Applied Optics

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Cells and tissue imaging with digital holographic microscopy

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2003)

Christian Depeursinge Etienne Cuche Tristan Colomb Pia Dahlgren Anca M. Marian

The aim of Optical Digital Holography, applied to cells and tissue imaging, is to provide an accurate 3D imaging of biologic materials, down to the microscopic scale. The method has been developed to yield a very precise determination of cells and tissues morphology. Targeted accuracies are in the sub-micron range and allow for the observation of very small movements and deformations, produced, in particular, by depolarization of excitable cells and their metabolic activities. Direct imaging of tissue structures by the newly developed digital holography is deemed to offer unique investigation means in biology and medicine and attractive diagnostic capabilities.

Digital Holographic Microscopy

Digital holography

10.1117/12.500899

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Citations (3)

Digital holographic microscopy (DHM) for metrology and dynamic characterization of MEMS and MOEMS

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2006)

Yves Emery Etienne Cuche François Marquet Nicolas Aspert Pierre Marquet

Digital Holographic Microscopes (DHM) enables recording the whole information necessary to provide real time nanometric vertical displacement measurements with a single image acquisition. The use of fast acquisition camera or stroboscopic acquisition mode makes these new systems ideal tools for investigating the topography and dynamical behavior of MEMS and MOEMS. This is illustrated by the investigation of resonant frequencies of a dual axis micromirror. This enables the definition of the linear, non-linear, and modal resonance zones of its dynamical response.

Digital Holographic Microscopy

Digital holography

Characterization

Holographic interferometry

10.1117/12.660029

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Citations (19)

Numerical optics in digital holography

Adaptive Optics: Analysis and Methods/Computational Optical Sensing and Imaging/Information Photonics/Signal Recovery and Synthesis Topical Meetings on CD-ROM (2007)

Tristan Colomb Florian Charrière Jonas Kühn Frédéric Montfort Christian Depeursinge

As digital holography achieves numerical reconstruction of the complex wavefront diffracted by specimen, a numerical optics formalism has been developed: numerical filters replace pinholes in Fourier planes, numerical lenses allow aberrations compensation and image magnification.

Digital holography

Formalism (music)

Numerical aperture

Physical optics

10.1364/dh.2007.dtuc4

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Citations (0)

Jones vector imaging by use of digital holography: simulation and experimentation

Optics Communications (2003)

Tristan Colomb Etienne Cuche Frédéric Montfort Pierre Marquet C. Depeursinge

Digital holography

10.1016/j.optcom.2003.12.004

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Citations (48)

Dual-wavelength digital holographic microscopy with sub-nanometer axial accuracy

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2008)

Jonas Kühn Florian Charrière Tristan Colomb Frédéric Montfort Etienne Cuche

We present dual-wavelength Digital Holographic Microscopy (DHM) measurements on a certified 8.9 nm high Chromium thin step sample and demonstrate sub-nanometer axial accuracy. We introduce a modified DHM Reference Calibrated Hologram (RCH) reconstruction algorithm taking into account amplitude contributions. By combining this with a temporal averaging procedure and a specific dual-wavelength DHM arrangement, it is shown that specimen topography can be measured with an accuracy, defined as the axial standard deviation, reduced to at least 0.9 nm. Indeed, it is reported that averaging each of the two wavefronts recorded with real-time dual-wavelength DHM can provide up to 30% spatial noise reduction for the given configuration, thanks to their non-correlated nature.

Digital Holographic Microscopy

Digital holography

Nanometre

10.1117/12.781263

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Tomography of red blood cells by multiple-wavelength digital holographic microscopy

Jonas Kühn Frédéric Montfort Tristan Colomb Benjamin Rappaz Corinne Moratal

We present a method enabling cell tomography by multiple-wavelength digital holographic microscopy. 20 reflection holograms between 485 and 670 nm are sequentially acquired, then the wavefronts are summed to obtain 3D tomographic reconstruction of red blood cells.

Digital Holographic Microscopy

Digital holography

Reflection

10.1364/dh.2009.dtua3

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Use of digital holographic microscopy in tomography

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2006)

Florian Charrière Frédéric Montfort Jonas Kühn Tristan Colomb Anca Marian

Digital Holographic Microscopy (DHM) provides three-dimensional (3D) images with a high vertical accuracy in the nanometer range and a diffracted limited transverse resolution. This paper focuses on 3 different tomographic applications based on DHM. First, we show that DHM can be combined with time gating: a series of holograms is acquired at different depths by varying the reference path length, providing after reconstruction images of slices at different depths in the specimen thanks to the short coherence length of the light source. Studies on enucleated porcine eyes will be presented. Secondly, we present a tomography based on the addition of several reconstructed wavefronts measured with DHM at different wavelengths. Each wavefront phase is individually adjusted to be equal in a given plane of interest, resulting in a constructive addition of complex waves in the selected plane and destructive addition in the others. Varying the plane of interest enables the scan of the object in depth. Thirdly, DHM is applied to perform optical diffraction tomography of a pollen grain: transmission phase images are acquired for different orientations of the rotating sample, then the 3D refractive index spatial distribution is computed by inverse radon transform. The presented works will exemplify the versatility of DHM, but above all its capability of providing quantitative tomographic data of biological specimen in a quick, reliable and non-invasive way.

Digital Holographic Microscopy

Digital holography

Tomographic reconstruction

Diffraction tomography

10.1117/12.662011

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Citations (2)

Digital Holographic Microscopy for Comparative Metrology in Micro-Optics and MOEMs (Digitale holografische Mikroskopie für vergleichende Messungen von mikro-optischen Komponenten und MOEMs)

tm - Technisches Messen (2006)

Christian Depeursinge Florian Charrière Frédéric Montfort

Summary Digital holographic microscopy (DHM) is an imaging technique, which can be applied to the visualisation and metrology of microscopic objects. It has been established in the frame of the DISCO project that DHM can be considered as an efficient method for direct comparison of the shape or deformations of two objects. “Comparison DHM” allows a straight comparison with a gauge provided by a manufacturer or customer. The phase subtraction between ideal and actual wavefront of the object provides data equivalent to those given by a Twyman–Green or a Mach–Zehnder interferometer.

Digital Holographic Microscopy

Digital holography

Holographic interferometry

10.1524/teme.2006.73.3.172

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Citations (2)

Numerical parametric lens for shifting, magnification, and complete aberration compensation in digital holographic microscopy

Journal of the Optical Society of America A (2006)

Tristan Colomb Frédéric Montfort Jonas Kühn Nicolas Aspert Etienne Cuche

The concept of numerical parametric lenses (NPL) is introduced to achieve wavefront reconstruction in digital holography. It is shown that operations usually performed by optical components and described in ray geometrical optics, such as image shifting, magnification, and especially complete aberration compensation (phase aberrations and image distortion), can be mimicked by numerical computation of a NPL. Furthermore, we demonstrate that automatic one-dimensional or two-dimensional fitting procedures allow adjustment of the NPL parameters as expressed in terms of standard or Zernike polynomial coefficients. These coefficients can provide a quantitative evaluation of the aberrations generated by the specimen. Demonstration is given of the reconstruction of the topology of a microlens.

Digital Holographic Microscopy

Optical aberration

Distortion (music)

Digital holography

10.1364/josaa.23.003177

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Citations (322)

Characterization of microlenses by digital holographic microscopy

Applied Optics (2006)

Florian Charrière Jonas Kühn Tristan Colomb Frédéric Montfort Etienne Cuche

We demonstrate the use of digital holographic microscopy (DHM) as a metrological tool in micro-optics testing. Measurement principles are compared with those performed with Twyman-Green, Mach-Zehnder, and white-light interferometers. Measurements performed on refractive microlenses with reflection DHM are compared with measurements performed with standard interferometers. Key features of DHM such as digital focusing, measurement of shape differences with respect to a perfect model, surface roughness measurements, and optical performance evaluation are discussed. The capability of imaging nonspherical lenses without any modification of the optomechanical setup is a key advantage of DHM compared with conventional measurement tools and is demonstrated on a cylindrical microlens and a square lens array.

Microlens

Digital Holographic Microscopy

Achromatic lens

Characterization

Holographic interferometry

Digital holography

10.1364/ao.45.000829

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Citations (210)