Yong Lai

Henan Agricultural University

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Timothy K. Lu

Massachusetts Institute of Technology

Erjing Si

Gansu Agricultural University

Xiaole Ma

Gansu Agricultural University

Yaxiong Meng

Gansu Agricultural University

Juncheng Wang

Chinese PLA General Hospital

Dangquan Zhang

Henan Agricultural University

Huajun Wang

Gansu Agricultural University

Xunwu Shang

Gansu Agricultural University

Baochun Li

University of Toronto

Yuhua Kong

Henan Agricultural University

Cooperative Institutions

Henan Agricultural University

Massachusetts Institute of Technology

Central South University

Chinese Academy of Sciences

Harvard University

Gansu Agricultural University

Central South University of Forestry and Technology

Sun Yat-sen University

Brigham and Women's Hospital

University of Hong Kong

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Identification and selection of low-phosphate-tolerant germplasm in barley (Hordeum vulgare L.)

Soil Science & Plant Nutrition (2016)

Panrong Ren Xiaole Ma Baochun Li Yaxiong Meng Yong Lai

Phosphorus (P) deficiency is one of the major constraints to crop yield worldwide, and genotypes or cultivars with high phosphate use efficiency (PUE) sustain growth when exposed to phosphate stress. Therefore, it is imperative to develop the genotypes or cultivars with high PUE. A pot experiment was conducted to evaluate the PUE among 150 barley (Hordeum vulgare L.) genotypes. Two high-tolerant and -sensitive accessions were selected. These two candidate materials were used to investigate the differences among the root morphology characteristics, antioxidant enzyme activity, inorganic phosphate (Pi) content and gene expression of HvPT5 under P-deficiency and P-sufficiency conditions. The values of these parameters were higher in the low-P-tolerant genotype than in the sensitive one. In pot experiment 1, all genotypes showed a significant difference in low-P tolerance, with variety GN121 achieving the highest tolerance, and GN42 being most sensitive. The results of this study may provide elite genetic germplasms for future work on isolation of P-related genes, and the improvement of PUE in barley.Abbreviations: PUE: phosphate use efficiency; CAT: catalase; POD: peroxidase; SOD: superoxide dismutase; DMSO: dimethyl sulphoxide; MDA: malondialdehyde; TOPSIS: technique for order preference by similarity to an ideal solution; MCDM/MADM: multi-criteria (or attribute) decision making

Germ plasm

10.1080/00380768.2016.1223521

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Citations (9)

Constituent diversity of ethanol extracts from pitaya

Asia-Pacific Journal of Chemical Engineering (2020)

Jinghua Ma Wei Mo Pangpan Zhang Yong Lai Ximei Li

Abstract Pitaya is rich in nutrition and unique in function and contains vegetable albumin, anthocyanins, rich vitamins, and water soluble dietary fiber, which are rare in fruits and vegetables. However, researches mostly focused on pitaya cultivation, biological characteristics, fresh‐keeping, and lacked analyses about chemical composition of pitaya. Therefore, ethanol extraction and GC/MS were used to extract and identify the bioactive components in pulp and pericarp of red pitaya and white pitaya. The extracting results showed that percentages of ethanol extracts from pulp and pericarp of red pitaya were 14.5% and 12%, whereas those of white pitaya were 16% and 12%. The GC/MS results showed that different kinds of bioactive constituents were identified in fresh pericarp and pulp of red pitaya and white pitaya. In the four ethanol extracts, there are rich polysiloxane plant organosilicon with total contents of 10.09%, 24.21%, 25.77%, and 23.25%. Meanwhile, there are many heterocyclic substances, and the content of pericarp is much higher than that of pulp. The four ethanol extracts also contain some other bioactive ingredients, such as esters, hydrocarbons, alcohols, amides, halides, and fatty acids, which can be used as raw materials for biomedicine, advanced cosmetics, and skin care products.

10.1002/apj.2478

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Citations (3)

Illuminating Host-Mycobacterial Interactions with Genome-wide CRISPR Knockout and CRISPRi Screens

Cell Systems (2020)

Yong Lai Gregory H. Babunovic Liang Cui Peter C. Dedon John G. Doench

CRISPR interference

10.1016/j.cels.2020.08.010

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Citations (34)

A synthetic transcription platform for programmable gene expression in mammalian cells

Nature Communications (2022)

Chien‐Wen Chen Leonid Gaidukov Yong Lai Ming-Ru Wu Jicong Cao

Abstract Precise, scalable, and sustainable control of genetic and cellular activities in mammalian cells is key to developing precision therapeutics and smart biomanufacturing. Here we create a highly tunable, modular, versatile CRISPR-based synthetic transcription system for the programmable control of gene expression and cellular phenotypes in mammalian cells. Genetic circuits consisting of well-characterized libraries of guide RNAs, binding motifs of synthetic operators, transcriptional activators, and additional genetic regulatory elements express mammalian genes in a highly predictable and tunable manner. We demonstrate the programmable control of reporter genes episomally and chromosomally, with up to 25-fold more activity than seen with the EF1α promoter, in multiple cell types. We use these circuits to program the secretion of human monoclonal antibodies and to control T-cell effector function marked by interferon-γ production. Antibody titers and interferon-γ concentrations significantly correlate with synthetic promoter strengths, providing a platform for programming gene expression and cellular function in diverse applications.

Synthetic Biology

Transcription

HEK 293 cells

10.1038/s41467-022-33287-9

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Citations (17)

Physiological and proteomic analyses of salt stress response in the halophyte Halogeton glomeratus

Plant Cell & Environment (2014)

Juncheng Wang Yaxiong Meng Baochun Li Xiaole Ma Yong Lai

Very little is known about the adaptation mechanism of Chenopodiaceae Halogeton glomeratus, a succulent annual halophyte, under saline conditions. In this study, we investigated the morphological and physiological adaptation mechanisms of seedlings exposed to different concentrations of NaCl treatment for 21 d. Our results revealed that H. glomeratus has a robust ability to tolerate salt; its optimal growth occurs under approximately 100 mm NaCl conditions. Salt crystals were deposited in water-storage tissue under saline conditions. We speculate that osmotic adjustment may be the primary mechanism of salt tolerance in H. glomeratus, which transports toxic ions such as sodium into specific salt-storage cells and compartmentalizes them in large vacuoles to maintain the water content of tissues and the succulence of the leaves. To investigate the molecular response mechanisms to salt stress in H. glomeratus, we conducted a comparative proteomic analysis of seedling leaves that had been exposed to 200 mm NaCl for 24 h, 72 h and 7 d. Forty-nine protein spots, exhibiting significant changes in abundance after stress, were identified using matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS/MS) and similarity searches across EST database of H. glomeratus. These stress-responsive proteins were categorized into nine functional groups, such as photosynthesis, carbohydrate and energy metabolism, and stress and defence response.

10.1111/pce.12428

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Citations (105)

Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA

bioRxiv (Cold Spring Harbor Laboratory) (2019)

Jin Wang Bing Liang Alvin Chew Yong Lai Hongping Dong Luang Xu

ABSTRACT Chemical modification of transcripts with 5’ caps occurs in all organisms. Here we report a systems-level mass spectrometry-based technique, CapQuant, for quantitative analysis of the cap epitranscriptome in any organism. The method was piloted with 21 canonical caps – m 7 GpppN, m 7 GpppNm, GpppN, GpppNm, and m 2,2,7 GpppG – and 5 “metabolite” caps – NAD, FAD, UDP-Glc, UDP-GlcNAc, and dpCoA. Applying CapQuant to RNA from purified dengue virus, Escherichia coli , yeast, mice, and humans, we discovered four new cap structures in humans and mice (FAD, UDP-Glc, UDP-GlcNAc, and m 7 Gpppm 6 A), cell- and tissue-specific variations in cap methylation, and surprisingly high proportions of caps lacking 2’- O -methylation, such as m 7 Gpppm 6 A in mammals and m 7 GpppA in dengue virus, and we did not detect cap m 1 A/m 1 Am in humans. CapQuant accurately captured the preference for purine nucleotides at eukaryotic transcription start sites and the correlation between metabolite levels and metabolite caps. The mystery around cap m 1 A/m 1 Am analysis remains unresolved.

Transcription

10.1101/683045

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Citations (10)

Efficacy and Antifungal Mechanism of Rosemary Essential Oil against Colletotrichum gloeosporioides

Forests (2024)

Tiantian Yuan Hua Yang Dangquan Zhang Chao‐Chen Yang Yong Lai

The antifungal activity and mechanism of rosemary essential oil against Colletotrichum gloeosporioides, the walnut anthracnose pathogen, were investigated using scanning electron microscopy (SEM), index determination and transcriptome technique. The results showed that rosemary essential oil could inhibit the growth of C. gloeosporioides with minimum inhibitory (MIC) and fungicidal (MFC) concentrations of 15.625 μL/mL and 31.25 μL/mL, respectively. Scanning electron microscopy revealed that the mycelium morphology became shriveled, twisted, and severely deformed after being treated with rosemary essential oil. The activity of chitinase, which decomposes fungal cell wall components in C. gloeosporioides, increased. The ergosterol content in the plasma membrane decreased, while the cell contents including nucleic acids, soluble protein and soluble reducing sugar were released resulting in the extracellular electrical conductivity being changed. For metabolic activity, the enzymes succinate dehydrogenase (SDH), malate dehydrogenase (MDH), ATPase and ATP decreased, whereas phosphofructokinase (PFK) increased. Transcriptome sequencing results showed that the antifungal mechanism of rosemary essential oil involves the destruction of the cell wall and membrane, inhibition of genetic material synthesis, and cell division and differentiation. The results are helpful to understand the efficacy and antifungal mechanism of rosemary essential oil against C. gloeosporioides and provide a theoretical basis for the development of rosemary essential oil as a biological control agent.

10.3390/f15020377

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Citations (7)

Effect of stand age on the temporal dynamics of soil active carbon and nitrogen in Chinese cypress artificial forests

Soil Science & Plant Nutrition (2022)

Shanshan Gong Xiaoyan Yang Xingkai Xu Yong Lai Zhihua Zhang

Stand age is a key factor for reforestation, and it has an important influence on the sustainable development of artificial forests. In order to reveal the temporal dynamics of soil active carbon (C) and nitrogen (N) in Chinese cypress artificial forests (CAFs) under different stand ages, soil samples were collected from five stands (25, 35, 45, 55, and 65 years old) in rocky mountainous of North China. The characterization of soil active C and N components, like soil microbial biomass C (MBC), microbial biomass N (MBN), dissolved organic C (DOC), dissolved organic N (DON), ammonium-N (NH4+-N) and nitrate-N (NO3−-N), as well as other soil properties were determined. The results showed that with the increase of stand age, the contents of soil MBC, MBN, DOC, DON, NH4+-N and NO3−-N tended to increase first and then decrease, and most of them reached the highest values in 45 years old. Soil active C and N components all reached peak values in June, and all of them showed the most significant variations in 0 ~ 10 cm soil layer (P < 0.01). Correlation analysis revealed that soil active C and N components showed significantly positive correlations with soil total N, total C, the number of microorganism communities and soil water content, as well as litter biomass (P < 0.01). Stepwise multiple regression analysis demonstrated that soil total N was the key controlling factor to soil active C and N components. In conclusion, with the increase of stand ages, soil active C and N components of CAFs did not accumulate all the time, indicating that stand age should be regarded as an important factor for the sustainable management of artificial forests.

Litter

Soil carbon

Soil test

Plant litter

10.1080/00380768.2022.2030194

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Citations (4)

Synthesis of [2-(3,4-epoxycyclohexyl) ethyl] triphenylsilane and study on its amine curing properties

Materials Science-Poland (2024)

Yanling Yu Biwu Huang Yafen Qu Junshan Qiu Yong Lai

Abstract [2-(3,4-epoxycyclohexyl) ethyl] triphenylsilane was synthesized, using triphenylsilane and 1,2-epoxy-4-vinylcyclohexane as the main raw materials. When the molar ratio of triphenylsilane to 1,2-epoxy-4-vinylcyclohexane was 1.0:1.2, the content of Wilkinson catalyst was 0.4%, the reaction temperature was 90°C, and the reaction time was 6 hours, the yield of [2-(3,4-epoxycyclohexyl) ethyl] triphenylsilane could reach 95.21%. The structure of the synthesized product was analyzed and characterized using FT-IR and 1H-NMR. The synthesized product was added to a bisphenol A-type epoxy resin (E-51) and a modified amine (593 amine) to prepare an adhesive. Then, the adhesive was poured into the mold and cured at 35°C for 8 hours. The cured sample exhibited the best performance when the ECETPS: E-51:593 amine molar ratio was 0.8:7.2:2. Thermal gravimetric analysis (TG) showed that the thermal stability of the cured samples increased relative to pure E-51 amine-cured samples with the synthesized product added. The mechanical properties of the samples were tested using a universal material testing machine, and the results showed a tensile strength of 37.95 MPa and a bending strength of 39.10 MPa.

Thermal Stability

Molar ratio

Thermogravimetric analysis

10.2478/msp-2024-0020

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Engineering Microbial Consortia as Living Materials: Advances and Prospectives

ACS Synthetic Biology (2024)

Shuchen Wang Yuewei Zhan Xue Jiang Yong Lai

The field of Engineered Living Materials (ELMs) integrates engineered living organisms into natural biomaterials to achieve diverse objectives. Multiorganism consortia, prevalent in both naturally occurring and synthetic microbial cultures, exhibit complex functionalities and interrelationships, extending the scope of what can be achieved with individual engineered bacterial strains. However, the ELMs comprising microbial consortia are still in the developmental stage. In this Review, we introduce two strategies for designing ELMs constituted of microbial consortia: a top-down strategy, which involves characterizing microbial interactions and mimicking and reconstructing natural ecosystems, and a bottom-up strategy, which entails the rational design of synthetic consortia and their assembly with material substrates to achieve user-defined functions. Next, we summarize technologies from synthetic biology that facilitate the efficient engineering of microbial consortia for performing tasks more complex than those that can be done with single bacterial strains. Finally, we discuss essential challenges and future perspectives for microbial consortia-based ELMs.

Synthetic Biology

10.1021/acssynbio.4c00313

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Citations (2)