Emma Huxley

Research Field

Genomic Copy Number Aberrations and Copy Neutral Loss of Heterozygosity Evaluation in Myeloid Neoplasms: Evidence-Based Recommendations for Clinical Genetic Testing From the Myeloid Malignancies Working Group of the Cancer Genomics Consortium

Cancer Genetics (2017)

Jennelle C. Hodge Rashmi Kanagal‐Shamanna Xinjie Xu Christine R. Bryke Durga Prasad Dash

SNP array

10.1016/j.cancergen.2017.04.017

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Citations (1)

Assessing copy number abnormalities and copy-neutral loss-of-heterozygosity across the genome as best practice in diagnostic evaluation of acute myeloid leukemia: An evidence-based review from the cancer genomics consortium (CGC) myeloid neoplasms working group

Xinjie Xu Christine R. Bryke Madina Sukhanova Emma Huxley Durga Prasad Dash

Abstract Structural genomic abnormalities, including balanced chromosomal rearrangements, copy number gains and losses and copy-neutral loss-of-heterozygosity (CN-LOH) represent an important category of diagnostic, prognostic and therapeutic markers in acute myeloid leukemia (AML). Genome-wide evaluation for copy number abnormalities (CNAs) is at present performed by karyotype analysis which has low resolution and is unobtainable in a subset of cases. Furthermore, examination for possible CN-LOH in leukemia cells is at present not routinely performed in the clinical setting. Chromosomal microarray (CMA) analysis is a widely available assay for CNAs and CN-LOH in diagnostic laboratories, but there are currently no guidelines how to best incorporate this technology into clinical testing algorithms for neoplastic diseases including AML. The Cancer Genomics Consortium Working Group for Myeloid Neoplasms performed an extensive review of peer-reviewed publications focused on CMA analysis in AML. Here we summarize evidence regarding clinical utility of CMA analysis in AML extracted from published data, and provide recommendations for optimal utilization of CMA testing in the diagnostic workup. In addition, we provide a list of CNAs and CN-LOH regions which have documented clinical significance in diagnosis, prognosis and treatment decisions in AML.

Copy number analysis

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Clonal evolution in adult TCF3::HLF-positive acute lymphoblastic leukemia undergoing stem cell transplantation

Annals of Hematology (2022)

Maria Zahid Ahmed Indrani Venkatadasari Sara Dyer Kerry Wall Emma Huxley

Hematology

Acute lymphocytic leukemia

10.1007/s00277-022-04941-5

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Validation of Whole Genome SNP Array As the Primary Genetic Test in Myelodysplastic Syndrome, to Replace Chromosome and FISH Copy Number Analysis, in a UK Diagnostic Genetic Laboratory

Blood (2016)

Sally Jeffries Nicola Trim Emma Huxley Judith Caddick Jane Soden

SNP array

Copy number analysis

SNP

10.1182/blood.v128.22.3184.3184

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Genetic Analysis Using a High Density SNP Array in Myelodysplastic Syndrome: Clinical Utility and Comparative Analysis Study Compared to Metaphase Chromosome Analysis

Blood (2015)

Sally Jeffries Nicola Trim Emma Huxley Laura Ford Manoj Raghavan

SNP array

Comparative genomic hybridization

Genetic Analysis

10.1182/blood.v126.23.5259.5259

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Citations (1)

Assessing copy number aberrations and copy neutral loss of heterozygosity across the genome as best practice: An evidence based review of clinical utility from the cancer genomics consortium (CGC) working group for myelodysplastic syndrome, myelodysplastic/myeloproliferative and myeloproliferative neoplasms

Rashmi Kanagal‐Shamanna Jennelle C. Hodge Tracy Tucker Shashi Shetty Ashwini Yenamandra

Abstract Multiple studies have demonstrated the utility of chromosomal microarray (CMA) testing to identify clinically significant copy number alterations (CNAs) and copy-neutral loss-of-heterozygosity (CN-LOH) in myeloid malignancies. However, guidelines for integrating CMA as a standard practice for diagnostic evaluation, assessment of prognosis and predicting treatment response are still lacking. CMA has not been recommended for clinical work-up of myeloid malignancies by the WHO 2016 or the NCCN 2017 guidelines but is a suggested test by the European LeukaemiaNet 2013 for the diagnosis of primary myelodysplastic syndrome (MDS). The Cancer Genomics Consortium (CGC) Working Group for Myeloid Neoplasms systematically reviewed peer-reviewed literature to determine the power of CMA in (1) improving diagnostic yield, (2) refining risk stratification, and (3) providing additional genomic information to guide therapy. In this manuscript, we summarize the evidence base for the clinical utility of array testing in the workup of MDS, myelodysplastic/myeloproliferative neoplasms (MDS/MPN) and myeloproliferative neoplasms (MPN). This review provides a list of recurrent CNAs and CN-LOH noted in this disease spectrum and describes the clinical significance of the aberrations and how they complement gene mutation findings by sequencing. Furthermore, for new or suspected diagnosis of MDS or MPN, we present suggestions for integrating genomic testing methods (CMA and mutation testing by next generation sequencing) into the current standard-of-care clinical laboratory testing (karyotype, FISH, morphology, and flow).

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Cancer Genetics (2018)

Xinjie Xu Christine R. Bryke Madina Sukhanova Emma Huxley Durga Prasad Dash

Copy number analysis

10.1016/j.cancergen.2018.07.005

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Citations (25)

Cancer Genetics (2018)

Rashmi Kanagal‐Shamanna Jennelle C. Hodge Tracy Tucker Shashi Shetty Ashwini Yenamandra

Personalized Medicine

10.1016/j.cancergen.2018.07.003