Hu‐Lin Jiang

China Pharmaceutical University

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Lei Xing

China Pharmaceutical University

Chong‐Su Cho

Seoul National University

Myung‐Haing Cho

NSF’s NOIRLab

Tian‐Jiao Zhou

China Pharmaceutical University

You-Kyoung Kim

Asan Medical Center

Peng‐Fei Cui

Changzhou University

Yu‐Jing He

Zhejiang Chinese Medical University

Rohidas Arote

Jain University

Yun‐Jaie Choi

Seoul National University

Jian-Bin Qiao

China Pharmaceutical University

Cooperative Institutions

China Pharmaceutical University

160

Seoul National University

133

Chinese Academy of Sciences

State Key Laboratory of Natural Medicine

Yeungnam University

Huazhong University of Science and Technology

Central South University

Zhejiang University

Shanghai Jiao Tong University

Chinese Academy of Medical Sciences & Peking Union Medical College

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An Advanced Multivalent Ligand‐Decorated Microsphere Enrichment System Efficiently Captures Circulating Tumor Cells In Vivo

Small (2025)

Xiaojie Wang Yuan Ji Jun Luo Ying Sun Tian‐Jiao Zhou

Abstract Capturing circulating tumor cells (CTCs) in vivo from the bloodstream lessens tumor metastasis and recurrence risks. However, the absence of CTC receptors due to epithelial‐mesenchymal transition (EMT), the limited binding capacity of a single ligand, and the complexity of the blood flow environment significantly reduce the efficiency of CTC capture in vivo. Herein, a multivalent ligand‐decorated microsphere enrichment system (MLMES) is crafted that incorporates a capture column replete with an immunosorbent that precisely recognizes and binds the stably expressed cluster of differentiation 44 (CD44) and glucose transporter protein 1 (GLUT1) receptors present on the exterior of CTCs. As peripheral blood flows through the column, CTCs are efficiently captured, achieving an in vivo capture rate of up to 64.2%, the highest reported to date. Moreover, the MLMES demonstrates excellent biocompatibility, broad‐spectrum tumor cells capture, and storage stability. Importantly, it significantly eliminates a substantial quantity of CTCs from peripheral blood, reducing the risk of metastasis. This breakthrough method has broad clinical application potential in preventing tumor metastasis and recurrence, bringing new possibilities for improving cancer treatment.

Circulating tumor cell

Biocompatibility

10.1002/smll.202409036

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An Autologous Macrophage‐Based Phenotypic Transformation‐Collagen Degradation System Treating Advanced Liver Fibrosis

Advanced Science (2023)

Bo‐Wen Duan Yanjun Liu Xue‐Na Li Meng‐Meng Han Hao‐Yuan Yu

Abstract In advanced liver fibrosis (LF), macrophages maintain the inflammatory environment in the liver and accelerate LF deterioration by secreting proinflammatory cytokines. However, there is still no effective strategy to regulate macrophages because of the difficulty and complexity of macrophage inflammatory phenotypic modulation and the insufficient therapeutic efficacy caused by the extracellular matrix (ECM) barrier. Here, AC73 and siUSP1 dual drug‐loaded lipid nanoparticle is designed to carry milk fat globule epidermal growth factor 8 (MFG‐E8) (named MUA/Y) to effectively inhibit macrophage proinflammatory signals and degrade the ECM barrier. MFG‐E8 is released in response to the high reactive oxygen species (ROS) environment in LF, transforming macrophages from a proinflammatory (M1) to an anti‐inflammatory (M2) phenotype and inducing macrophages to phagocytose collagen. Collagen ablation increases AC73 and siUSP1 accumulation in hepatic stellate cells (HSCs) and inhibits HSCs overactivation. Interestingly, complete resolution of liver inflammation, significant collagen degradation, and HSCs deactivation are observed in methionine choline deficiency (MCD) and CCl 4 models after tail vein injection of MUA/Y. Overall, this work reveals a macrophage‐focused regulatory treatment strategy to eliminate LF progression at the source, providing a new perspective for the clinical treatment of advanced LF.

Proinflammatory cytokine

Hepatic stellate cell

10.1002/advs.202306899

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Citations (12)

Synergistic effects of Akt1 shRNA and paclitaxel-incorporated conjugated linoleic acid-coupled poloxamer thermosensitive hydrogel on breast cancer

Biomaterials (2011)

Ding‐Ding Guo Seong‐Ho Hong Hu‐Lin Jiang Jihye Kim Arash Minai‐Tehrani

Combination therapy

10.1016/j.biomaterials.2011.12.011

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Citations (46)

Immobilization of Heparin to Galactosylated Chitosan as a Synthetic Extracellular Matrix for Liver Tissue Engineering

Hu‐Lin Jiang Seog-Jin Seo Takashi Hoshiba Toshihiro Akaike Chong‐Su Cho

Galactose moiety was covalently coupled with chitosan to improve hepatocyte adhesion and heparin was immobilized to the galactosylated chitosan (GC) to enhancing hepatocyte function. Hepatocyte adhesion to heparin immobilized GC (GCH)-casted polystyrene (PS) dish was higher than that to chitosan-casted PS dish although it was slightly lower than GC-casted PS dish owing to the hydrated GCH-casted PS dish. Spreading of hepatocytes attached onto GCH-casted PS dish was increased with an increase of heparin for the GCH in the presence of hepatocyte growth factor (HGF). Also, DNA synthesis of the hepatocyte adhered to GCH-casted PS dish was increased with an increase of heparin in the GCH. Higher levels of albumin secretion were maintained on the GCH-casted PS dish than chitosan- or GC-casted PS dishes.

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Additional file 1: of Targeting the Hsp90-Cdc37-client protein interaction to disrupt Hsp90 chaperone machinery

Figshare (2018)

Ting Li Hu‐Lin Jiang Yunguang Tong Jin‐Jian Lu

The sup. Excel file for the comprehensive analysis of Hsp90-Cdc37’s client proteins. Comprehensive Analysis of Hsp90-Cdc37’s client proteins by using FunRich tool. (XLSX 313 kb)

CDC37

Chaperone (clinical)

10.6084/m9.figshare.6193598

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Recent Progress in Biomedical Applications of Chitosan Derivatives as Gene Carrier

Advances in polymer science (2021)

Pu-Song Zhao Yi Wang Wenshuang Sun Lian‐Yu Qi Li‐Fan Hu

Biocompatibility

Biosafety

10.1007/12_2021_106

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Citations (3)

Fabrication of stem cell heterospheroids with sustained-release chitosan and poly(lactic-co-glycolic acid) microspheres to guide cell fate toward chondrogenic differentiation

International Journal of Biological Macromolecules (2024)

Tiep Tien Nguyen Yun‐Seo Kil Jong‐Hyuk Sung Yu Seok Youn Ji Hoon Jeong

Glycolic acid

Chondrogenesis

10.1016/j.ijbiomac.2024.130356

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- Application of Marine Biomaterials for Gene Delivery

CRC Press eBooks (2013)

You-Kyoung Kim Hu‐Lin Jiang Bijay Singh Yun‐Jaie Choi Myung‐Haing Cho

In recent decades, gene therapy is a powerful method in curing a variety of inherited and acquired diseases by replacing defective genes, substituting missing genes, or silencing unwanted gene expression (Kabanov and Kabanov 1995). The lack of effective carriers is a major drawback to progress for clinical applications although most of the gene therapy relies on animal viral vectors because they achieve high efciency of gene transfer in vivo and in clinical trials. However, use of animal viral vectors has several limitations such as immunogenicity, potential infectivity, inammation to the human body, and complicated production (Smith 1995). Therefore, nonviral vectors have been rapidly receiving attention as a gene delivery system because of easy production, low immune response against the vector, and high exibility of size of the delivered gene (Mao et al. 2010). Among nonviral vectors, lipids and polymers are by far the most widely used gene carriers because theCONTENTS33.1 Introduction ........................................................................................................................ 657 33.2 Alginate ...............................................................................................................................658

10.1201/b14723-38

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Chemical Modification of Chitosan as a Gene Transporter

Current Organic Chemistry (2017)

Hu‐Lin Jiang Lei Xing Cheng-Qiong Luo Tian‐Jiao Zhou Hui-Shan Li

Background: Due to poor safety characterstics, viral vectors in gene delivery are usually limited in clinical application. Non-viral vectors have focused on the development of effective gene delivery carriers. As a promising non-viral carrier, chitosan (CS) is advantageous due to its biocompatibility, low toxicity and immunogenicity. Objective: The disadvantages such as low transfection efficiency and lack of celltargeting capability that CS possesses still need to be improved. Method: In this review, we summarized different chemical modifications of CS to overcome the obstacles mentioned before. The latest developments on the modifications of CS as gene delivery carriers will also be the main focus in this article. Results: For chemical modification of CS, the main interest focused on the ease of synthesis with controlled structure and size, biodegradability, high transfection efficiency and cell-targeted ability. Conclusion: Recent technological progress in chemical modification of CS has led to improvements of its transfection efficiency, better targeting specific cells and higher cellular uptake. Keywords: Chitosan, chitosan derivatives, gene delivery, DNA, siRNA, chemical modification.

Biocompatibility

Chemical modification

10.2174/1385272821666170926163544

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Citations (9)

Suppression of lung cancer progression by biocompatible glycerol triacrylate– spermine-mediated delivery of shAkt1

International Journal of Nanomedicine (2012)

Hu‐Lin Jiang Hồng Kim Kim Kim Kim Shin

Background: Polyethylenimine (PEI)-based nonviral gene-delivery systems are commonly employed because of their high transfection efficiency. However, the toxic nature of PEI is a significant obstacle in clinical gene therapy. In this study, we developed biocompatible glycerol triacrylate–spermine (GT–SPE) polyspermine as a nanosized gene carrier for potential lung cancer gene therapy. Methods: The GT–SPE was synthesized using the Michael addition reaction between GT and SPE. The molecular weight was characterized using gel permeability chromatography multiangle laser light scattering and the composition of the polymer was analyzed using proton nuclear magnetic resonance. Results: The GT–SPE successfully protected the DNA from nucleases. The average particle size of the GT–SPE was 121 nm with a zeta potential of +23.45 mV. The GT–SPE was found to be less toxic than PEI for various cell lines, as well as for a murine model. Finally, our results showed that the GT–SPE/small hairpin Akt1 (shAkt1) complex suppressed lung tumorigenesis in a K- ras LA1 lung cancer mice model by inducing apoptosis through the Akt signaling pathway and cell cycle arrest. Aerosol delivered GT–SPE/shAkt1, which reduced matrix metalloproteinase-9 activity and suppressed the expression levels of proliferating cell nuclear antigen, as well as vascular endothelial growth factors and CD31, which are known proliferation and angiogenesis markers, respectively. Conclusion: Our data suggest that GT–SPE may be a candidate for short hairpin-shaped RNA-based aerosol lung cancer gene therapy. Keywords: lung cancer, gene therapy, aerosol delivery, spermine Corrigendum has been published for this paper.

Biocompatible material

10.2147/ijn.s29152

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Citations (31)