Ramón Pérez‐Tanoira

Hospital Universitario Príncipe de Asturias

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Juan Cuadros

Hospital Universitario Príncipe de Asturias

Jaime Esteban

Hospital Universitario Fundación Jiménez Díaz

Laura Prieto-Pérez

Hospital Universitario Fundación Jiménez Díaz

Miguel Górgolas

Hospital Universitario Fundación Jiménez Díaz

Felipe Pérez‐García

Hospital Universitario Príncipe de Asturias

Peña Gómez-Herruz

Hospital Universitario Príncipe de Asturias

Enrique Gómez‐Barrena

Universidad Autónoma de Madrid

José Manuel Ramos

Universitat de Miguel Hernández d'Elx

Juan Romanyk

Hospital Universitario Príncipe de Asturias

Concepción Pérez‐Jorge

Universidad Autónoma de Madrid

Cooperative Institutions

Instituto de Salud Carlos III

288

Hospital Universitario La Paz

257

Universidad Autónoma de Madrid

222

Centro de Investigación Biomédica en Red

186

Museum of Heilongjiang Province

138

Hospital Universitario Fundación Jiménez Díaz

110

Universidad de Sevilla

Instituto de Biomedicina de Sevilla

Hospital La Paz Institute for Health Research

Hospital Universitario Virgen Macarena

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Urinary Tract Infection and Antimicrobial Susceptibility of Bacterial Isolates in Saint Joseph Kitgum Hospital, Kitgum, Uganda

Antibiotics (2022)

Félix Carrasco Calzada John Jairo Aguilera-Correa Juan Cuadros Jaime Esteban David Roca Biosca

A cross-sectional study of microorganisms isolated from mid-stream urine samples obtained from 139 patients with suspected urinary tract infection (UTI) who presented leukocyturia was conducted from April to June 2019 at Saint Joseph Kitgum Hospital (Uganda). All microorganisms were identified by MALDI-TOF mass spectrometry in a laboratory in Spain. Antimicrobial susceptibility was determined on site using the disc diffusion method (Kirby-Bauer test) and these results were subsequently compared with those obtained in Spain using the Becton Dickinson Phoenix M50 device. The overall prevalence of UTI with bacterial growth was 64.0% (n = 89) (95% CI, 56.1-72.0), and 11 presented mixed infection. As a result, 100 microorganisms were isolated. The most common uropathogens were Enterococcus spp. (57%) and Escherichia coli (28%). Nitrofurantoin was the most effective drug (81.7% in Gram-positive and 87.3% in Gram-negative bacteria), followed by imipenem (94.2% and 74.5%, respectively). The highest resistance rates were observed for amoxicillin and ciprofloxacin (66.2% and 44.6%, respectively). Given the increasing trend toward antibiotic resistance, there is a need for bacteriological cultures and continuous surveillance of uropathogen antibiotic susceptibility. Use of amoxicillin and ciprofloxacin as empirical treatments for UTIs should be discontinued in Uganda. The findings of this study may be useful for clinicians, as they may improve empirical treatment.

Nitrofurantoin

10.3390/antibiotics11040504

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Citations (8)

Global Dynamics of Gastrointestinal Colonisations and Antimicrobial Resistance: Insights from International Travellers to Low- and Middle-Income Countries

Tropical Medicine and Infectious Disease (2024)

Laura Seijas‐Pereda David Carmena Carlos Rescalvo-Casas Marcos Hernando-Gozalo Laura Prieto-Pérez

Gastrointestinal microorganism resistance and dissemination are increasing, partly due to international travel. This study investigated gastrointestinal colonisations and the acquisition of antimicrobial resistance (AMR) genes among international travellers moving between Spain and low- and middle-income countries (Peru and Ethiopia). We analysed 102 stool samples from 51 volunteers collected before and after travel, revealing significantly higher rates of colonisation by both bacteria and protists upon return. Diarrhoeagenic strains of E. coli were the most notable microorganism detected using RT-PCR with the Seegene Allplex™ Gastrointestinal Panel Assays. A striking prevalence of β-lactamase resistance genes, particularly the TEM gene, was observed both before and after travel. No significant differences in AMR genes were found between the different locations. These findings highlight the need for rigorous surveillance and preventive strategies, as travel does not significantly impact AMR gene acquisition but does affect microbial colonisations. This study provides valuable insights into the intersection of gastrointestinal microorganism acquisition and AMR in international travellers, underscoring the need for targeted interventions and increased awareness.

10.3390/tropicalmed9080182

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Citations (1)

Sanguiin H-6 Fractionated from Cloudberry (Rubus chamaemorus) Seeds Can Prevent the Methicillin-Resistant Staphylococcus aureus Biofilm Development during Wound Infection

Antibiotics (2021)

John Jairo Aguilera-Correa Sara Fernández López Iskra Dennisse Cuñas-Figueroa Sandra Pérez-Rial Hanna‐Leena Alakomi

Staphylococcus aureus is the most common cause of surgical site infections and its treatment is challenging due to the emergence of multi-drug resistant strains such as methicillin-resistant S. aureus (MRSA). Natural berry-derived compounds have shown antimicrobial potential, e.g., ellagitannins such as sanguiin H-6 and lambertianin C, the main phenolic compounds in Rubus seeds, have shown antimicrobial activity. The aim of this study was to evaluate the effect of sanguiin H-6 and lambertianin C fractionated from cloudberry seeds, on the MRSA growth, and as treatment of a MRSA biofilm development in different growth media in vitro and in vivo by using a murine wound infection model where sanguiin H-6 and lambertianin C were used to prevent the MRSA infection. Sanguiin H-6 and lambertianin C inhibited the in vitro biofilm development and growth of MRSA. Furthermore, sanguiin H-6 showed significant anti-MRSA effect in the in vivo wound model. Our study shows the possible use of sanguiin H-6 as a preventive measure in surgical sites to avoid postoperative infections, whilst lambertianin C showed no anti-MRSA activity.

10.3390/antibiotics10121481

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Comparative evaluation of Panbio and SD Biosensor antigen rapid diagnostic tests for COVID‐19 diagnosis

Journal of Medical Virology (2021)

Felipe Pérez‐García Juan Romanyk Helena Moya Gutiérrez Andrea Labrador Ballestero Inés Pérez Ranz

The aim of our study was to evaluate the diagnostic performance of two antigen rapid diagnostic tests (Ag-RDTs) to diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We evaluated Panbio and SD-Biosensor Ag-RDTs. We employed 186 polymerase chain reaction (PCR) negative samples to evaluate the specificity and 170 PCR positive samples to assess the sensitivity. We evaluated their sensitivity according to Cycle threshold (C t ) values and days post onset of symptoms (d.p.o.). Tests were compared using the McNemar's test. Agreement was evaluated using the kappa score. Specificity was 100% for Panbio and 97.3% for SD-Biosensor. Sensitivity for samples with C t ≤ 20 was 100% for both assays and for samples with C t = 20-25 was 93.0% (Panbio) and 95.3% (SD-Biosensor) (p = 1.000). Sensitivity decreased for samples wit C t = 25-30 (Panbio: 41.3%, SD-Biosensor: 52.2%, p = 0.125) and samples with C t ≥ 30 (Panbio: 5.0%, SD-Biosensor: 17.5%, p = 0.063). Sensitivity within seven d.p.o. was 87.7% for Panbio and 90.4% for SD-Biosensor and notably decreased after seven d.p.o. Agreement with PCR was excellent for high viral load samples (C t ≤ 25): Panbio, 98.9%, kappa = 0.974; SD-Biosensor, 97.4%, kappa = 0.940. Agreement between Ag-RDTs was excellent (94.9%, kappa = 0.882). Panbio and SD-Biosensor Ag-RDTs showed excellent agreement and diagnostic performance results for samples with high viral loads (C t ≤ 25) or samples within seven d.p.o.

McNemar's test

Kappa

10.1002/jmv.27089

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Citations (26)

"Race for the Surface" : Competition between Bacteria and Host Cells in Implant Colonization Process

Ramón Pérez‐Tanoira

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A bis(pyrazolyl)methane derivative against clinical Staphylococcus aureus strains isolated from otitis externa

Laryngoscope Investigative Otolaryngology (2022)

Ana V. Ocaña John Jairo Aguilera-Correa Elena Domínguez‐Jurado Francisco C. Pérez‐Martínez Ramón Pérez‐Tanoira

The purpose of this study was to evaluate the in vitro antibacterial effects of a

Minimum bactericidal concentration

Morganella morganii

Enterobacter aerogenes

10.1002/lio2.722

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Citations (3)

Leprosy ulcers in a rural hospital of Ethiopia: pattern of aerobic bacterial isolates and drug sensitivities

Annals of Clinical Microbiology and Antimicrobials (2014)

José Manuel Ramos Ramón Pérez‐Tanoira Cristina García-García Laura Prieto-Pérez María C Bellón

Plantar ulcers, which commonly occur in leprosy patients, tend to recur increasing physical disability. The aim of this study is to identify both the bacteriological profile of these ulcers and the antibiotic susceptibility of the isolated bacteria.68 leprosy patients with chronic ulcers attending the in-patient department of Gambo General Hospital, West Arsi, were included in this study. Proper sample collection, inoculation on culture media, and final identification using biochemical methods were undertaken.66 patients (97.1%) had a positive culture. A total of 81 microorganisms were isolated. Multiple organisms (two or more) were isolated in 15 (22.7% out of positive culture) patients. The main isolation was Proteus spp (30.9%), followed by Escherichia coli (21.0%), Staphylococcus aureus (18.5%) and Pseudomonas aeruginosa (9.9%). In the total number of the isolated bacteria, the antibiotics with less resistance were gentamicin (18.5%), fosfomycin (22.2%) cefoxitin (24.7%), ceftriaxone (25.9%) ciprofloxacin (25.9%), and amoxicillin-clavulanic acid (28.49%).The bacteriological study of plantar ulcers of leprosy patients revealed Enterobacteriaceae and S. aureus as the main pathogens involved in such infections. The results of this study may guide empirical therapy in a rural area hospital where culture and susceptibility testing facilities are scarce.

Fosfomycin

10.1186/s12941-014-0047-z

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Citations (17)

Histiocytic hyperplasia with hemophagocytosis and acute alveolar damage in COVID-19 infection

Modern Pathology (2020)

Laura Prieto-Pérez José Fortes Carlos Soto Ánxela Vidal-González Marina Alonso

Hemophagocytosis

Diffuse alveolar damage

CD68

Macrophage Activation Syndrome

10.1038/s41379-020-0613-1

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Citations (83)

Antimicrobial evaluation of quaternary ammonium polyethyleneimine nanoparticles against clinical isolates of pathogenic bacteria

IET Nanobiotechnology (2015)

Agustín Ortega Cerrato Shady Farah Pedro Tranque Ana V. Ocaña Syong Hyun Nam‐Cha

Peritonitis is a disease caused by bacterial strains that have become increasingly resistant to many antibiotics. The development of alternative therapeutic compounds is the focus of extensive research, so novel nanoparticles (NPs) with activity against antibiotic-resistant bacteria should be developed. In this study, the antibacterial activity of quaternary ammonium polyethyleneimine (QA-PEI) NPs was evaluated against Streptococcus viridans, Stenotrophomonas maltophilia and Escherichia coli. To appraise the antibacterial activity, minimal inhibitory concentration (MIC), minimal bactericidal concentration and bactericidal assays were utilised with different concentrations (1.56-100 µg/ml) of QA-PEI NPs. Moreover, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and annexin V/propidium iodide toxicity assays were performed in cell cultures. MICs for S. maltophilia and E. coli isolates were 12.5 and 25 µg/ml, respectively, whereas the MIC for S. viridans was 100 µg/ml. Furthermore, the growth curve assays revealed that these QA-PEI NPs at a concentration of 12.5 µg/ml significantly inhibited bacterial growth for the bacterial isolates studied. On the other hand, QA-PEI NPs lacked significant toxicity for cells when used at concentrations up to 50 μg/ml for 48 h. The present findings reveal the potential therapeutic value of this QA-PEI NPs as alternative antibacterial agents for peritonitis, especially against Gram-negative bacteria.

Stenotrophomonas maltophilia

Propidium iodide

Minimum bactericidal concentration

Pathogenic bacteria

Bacterial growth

10.1049/iet-nbt.2014.0078

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Citations (34)

Diagnóstico de gripe y otros virus respiratorios

Profesión veterinaria (2021)

Manuel Linares Ramón Pérez‐Tanoira Juan Romanyk Felipe Pérez García Peña Gómez Herruz

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