H. Chen

Women's Hospital, School of Medicine, Zhejiang University

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F. Ye

Zhejiang University

Xing Xie

Women's Hospital, School of Medicine, Zhejiang University

Weiguo Lü

Women's Hospital, School of Medicine, Zhejiang University

Die Hong

University of Southern California

Quan Cheng

Xiangya Hospital Central South University

Ying Hu

Harbin Institute of Technology

Weiguo Lü

Women's Hospital, School of Medicine, Zhejiang University

Qi Cheng

CVPath Institute

Feng Ye

Institute of Microbiology

J. Liu

Women's Hospital, School of Medicine, Zhejiang University

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Women's Hospital, School of Medicine, Zhejiang University

Zhejiang University

Central South University

Xiangya Hospital Central South University

Women's Hospital

Nanjing Medical University

Tongji Hospital

Shaanxi Provincial People's Hospital

Second Affiliated Hospital of Zhejiang University

Shandong University

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Interleukin-7 and suppression of local peritoneal immunity in ovarian carcinoma

International Journal of Gynecology & Obstetrics (2003)

Xing Xie D. Ye H. Chen W Lu Bei Cheng

Peritoneal fluid

Interleukin 8

Epithelial ovarian carcinoma

10.1016/j.ijgo.2003.08.015

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Citations (16)

Distribution of human papillomavirus genotypes in the patients with cervical carcinoma and its precursors in Zhejiang Province, China

International Journal of Gynecological Cancer (2008)

Die Hong F. Ye H. Chen Weiguo Lü Qi Cheng

Various evidences reveal that the human papillomavirus (HPV) is the single most important etiologic agent in cervical carcinoma (CC). To investigate the distribution of HPV genotypes in the patients with CC and its precursors in Zhejiang Province, China, a total of 631 eligible samples from patients in Zhejiang Province with CC ( N = 181), cervical intraepithelial neoplasia (CIN) II–III ( N = 345), and CIN I ( N = 105) were detected. Age-matched samples of 217 women without cervical neoplasia were detected as control. An improved polymerase chain reaction (PCR)–restriction fragment length polymorphism assay validated by Hybrid Capture II and PCR sequencing was designed for HPV genotype. The prevalence of HPV was 95.0% in CC, 88.4% in CIN II–III, and 73.3% in CIN I, while only 41.9% in control. High-risk/low-risk HPV ratio showed a significant trend of increase with increased grade of CIN and transformation to carcinoma. A total of 24 HPV genotypes were detected in CC and its precursors. Of those, HPV 16 (65.2%), 18 (9.4%), and 58 (9.4%) were the major HPV genotypes in CC, while HPV 16 (37.6%), 58 (19.1%), 33 (10.6%), and 18 (5.4%) in CIN. In conclusion, the distribution of predominant HPV genotypes in the patients with CC and its precursors in Zhejiang Province is HPV 16, 58, and 18, probably as well as 33, which may be high-risk factors for CC.

10.1136/ijgc-00009577-200801000-00016

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Evaluation of immune inhibitory cytokine profiles in epithelial ovarian carcinoma

Journal of Obstetrics and Gynaecology Research (2009)

Li‐li Chen Feng Ye Weiguo Lü Yan Yu H. Chen

The aim of this study was to detect expression of different cytokines in epithelial ovarian carcinoma (EOC) cells and normal ovarian surface epithelial (OSE) cells in vitro and the levels of those with elevated expression in the EOC patients, and to analyze the contribution of cytokine profiles to tumor immune deficiency.Cytokine antibody array was used to detect cytokine profiles in two cell lines of EOC (SKOV3 and CaoV3), primarily cultured EOC and OSE cells. The levels of leukemia inhibitory factor (LIF), interleukin-10 (IL-10), IL-4, and transforming growth factor-beta1 (TGF-beta1) in peritoneal fluids and sera in the patients with EOC and benign gynecological tumors were detected by enzyme-linked immunosorbent assay.The levels of LIF, IL-10, and IL-4 were detected two times higher in the culture supernatants of the EOC cell lines than those in OSE cells by cytokine antibody array. Both LIF and IL-10 levels were more increased in ascites of EOC patients than in those in benign gynecological tumor patients (P < 0.05). The level of IL-4 was not detectable in any samples of ascites or sera. No difference of TGF-beta1 value was detected between patients with EOC and benign gynecological tumors.Epithelium ovarian carcinoma cells can produce more LIF, IL-10 and IL-4 than OSE cells, and contribute to the elevated levels of those cytokines in EOC patients, which probably participates in the development of immune deficiency in the peritoneal cavity of EOC patients.

10.1111/j.1447-0756.2008.00935.x

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Expression of differentiation associated protein Hes1 and Hes5 in cervical squamous carcinoma and its precursors

International Journal of Gynecological Cancer (2007)

J. Liu F. Ye H. Chen Weiguo Lü Caiyun Zhou

Hairy and Enhancer-of-split homologues 1 and 5 (Hes1 and Hes5) are the basic helix-loop-helix transcriptional factors that negatively regulate the cell differentiation during embryogenesis. It has been reported that they may be involved in carcinogenesis in some tumors. The roles of Hes1 and Hes5 in development and progression of cervical carcinoma are not well documented todate. In the study, the expression of Hes1 and Hes5 were detected by immunohistochemistry in 295 cases with various degrees of cervical epithelial lesions, including 78 normal cervical epithelia, 31 mild dysplasia (CIN I), 77 moderate-severe dysplasia (CIN II–III), and 109 squamous cervical carcinomas (SCCs), and their association with various clinical pathologic prognostic variables were analyzed in 73 early-stage SCC patients who underwent surgery. Hes1 and Hes5 expression were found to be significantly higher in SCC compared with CIN as well as higher in CIN than normal cervical epithelia, and positively correlated with various prognostic factors in early-stage cervical carcinoma. Our findings suggest that Hes1 and Hes5 may be involved in carcinogenesis of the cervix and progression of cervical carcinoma. Hes1 and Hes5 overexpression are probably variables to predict poor prognosis of the patients with early-stage cervical carcinoma

HES1

10.1111/j.1525-1438.2007.00930.x

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Citations (19)

Distribution of human papillomavirus genotypes in the patients with cervical carcinoma and its precursors in Zhejiang Province, China

International Journal of Gynecological Cancer (2007)

Die Hong F. Ye H. Chen Weiguo Lü Quan Cheng

Various evidences reveal that the human papillomavirus (HPV) is the single most important etiologic agent in cervical carcinoma (CC). To investigate the distribution of HPV genotypes in the patients with CC and its precursors in Zhejiang Province, China, a total of 631 eligible samples from patients in Zhejiang Province with CC (N = 181), cervical intraepithelial neoplasia (CIN) II-III (N = 345), and CIN I (N = 105) were detected. Age-matched samples of 217 women without cervical neoplasia were detected as control. An improved polymerase chain reaction (PCR)-restriction fragment length polymorphism assay validated by Hybrid Capture II and PCR sequencing was designed for HPV genotype. The prevalence of HPV was 95.0% in CC, 88.4% in CIN II-III, and 73.3% in CIN I, while only 41.9% in control. High-risk/low-risk HPV ratio showed a significant trend of increase with increased grade of CIN and transformation to carcinoma. A total of 24 HPV genotypes were detected in CC and its precursors. Of those, HPV 16 (65.2%), 18 (9.4%), and 58 (9.4%) were the major HPV genotypes in CC, while HPV 16 (37.6%), 58 (19.1%), 33 (10.6%), and 18 (5.4%) in CIN. In conclusion, the distribution of predominant HPV genotypes in the patients with CC and its precursors in Zhejiang Province is HPV 16, 58, and 18, probably as well as 33, which may be high-risk factors for CC.

10.1111/j.1525-1438.2007.00968.x

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Citations (36)