Fibroblast activation protein (FAP), a cell surface serine protease, plays roles in tumor invasion and immune regulation. However, there is currently no pan-cancer analysis of FAP.
Laryngeal squamous cell carcinoma (LSCC) is a common head and neck cancer that is unresponsive to chemotherapy; therefore, understanding the causes of chemotherapy resistance is important. The cancer stem cell (CSC) theory postulates that CSCs are the source of tumor chemoresistance. We enrich laryngeal CSCs to overcome chemoresistance of LSCC. A laryngeal cancer xenograft model was established, and a low dose of cisplatin was administered until chemoresistance arose. A next-generation xenograft model was established using surviving tumor cells, and the test was repeated four times to screen for CSCs. Cell function experiments were performed on each tumor cell generation (m1, m2, m3, and m4). The m3 line, with the highest stemness, was selected for transcriptome sequencing. LY6D was selected for clinical sample validation and functional verification. LY6D expression was detected in 107 laryngeal cancer samples, with high expression in 91 of these samples. LY6D expression was correlated with pathological T and clinical stages, and with cervical lymph node metastasis. The siLY6D group exhibited reduced adhesion and chemoresistance to cisplatin, 5-fluorouracil, and paclitaxel. LY6D is upregulated in laryngeal cancer and may serve as a biomarker for chemoresistance in CSCs. Moreover, LY6D could serve as an alternative antigenic peptide in the targeted treatment of laryngeal cancer.
Objetive: To investigate whether the methylation patterns of the interleukin-4 (IL-4) gene promoter changed and whether environmental factors affected the methylation level of IL-4 gene in the peripheral blood of patients with recurrent aphthous ulcer (RAU). Methods: Totally 20 patients, who were diagnosed with RAU, were recruited from May 2018 to May 2019 in the Department of Stomatology， First Hospital of Shanxi Medical University in the study (RAU group), including 12 females and 8 males, with mean age of 16-35 years. During the same period, 20 healthy volunteers matching the age and gender of the RAU group were selected from the medical personnel of the same hospital as the healty control group, including 11 females and 9 males, with mean age of 15-35 years. Peripheral blood samples of two groups were collected and the methylation levels of the IL-4 promoter were detected by bisulfite sequencing PCR (BSP). The IL-4 promoter methylation level of each sample was analyzed by direct sequencing and the IL-4 mRNA level was detected by real-time quantitative PCR. The data obtained were statistically analyzed. Results: The IL-4 gene promoter fragment contained 10 CPG sites from -1400 to -1625 bp. The methylation rates of CPG(-1556), CPG(-1483), CPG(-1479)and 10 CPG sites were significantly higher in RAU group ［(32.0±19.9)%, (53.0±13.4)%, (46.0±19.8)% and (39.3±12.4)%］ than in healthy control group ［(20.0±3.2)%, (35.5±12.3)%, (28.0±14.4)% and (32.6±5.8)%］, with statistically significant differences (P<0.05). The relative expression of IL-4 mRNA in the peripheral blood of RAU patients (1.0±0.1) was significantly lower than that of the healthy control group (1.5±0.2) (P<0.01). There was a significant negative correlation between the overall methylation rate of IL-4 gene promoter and the relative expression level of IL-4 mRNA in RAU group (r=-0.494, P<0.05). In the multivariate analysis, smoking, vitamin B12 and folic acid in the RAU group were significantly correlated with the overall methylation rate of the IL-4 gene promoter (P<0.01). Conclusions: The hypermethylation of IL-4 promoter in RAU patients may be related to the reduction of IL-4 gene transcription. Vitamin B12, folic acid and smoking may affect IL-4 gene methylation in peripheral blood of RAU patients.目的： 探讨复发性阿弗他溃疡（recurrent aphthous ulcer，RAU）患者外周血中白细胞介素-4（interleukin-4，IL-4）基因启动子甲基化模式是否发生改变以及环境因素对RAU患者外周血中IL-4基因甲基化水平是否有影响。 方法： 采用调查研究，纳入2018年5月至2019年5月在山西医科大学第一医院口腔门诊就诊并诊断为RAU的患者20例（RAU组），其中女性12例，男性8例，年龄16~35岁。同期在医院体检人员中选取20名与RAU组年龄、性别匹配的健康志愿者作为健康对照组，其中女性11名，男性9名，年龄15~35岁。收集两组受试者外周血样本，采用亚硫酸氢盐处理后测序（bisulfite sequencing PCR，BSP）法检测IL-4启动子甲基化水平，采用实时荧光定量PCR技术检测IL-4 mRNA水平，对所得数据进行统计学分析。 结果： IL-4基因启动子片段从-1400至-1625 bp中含有10个CPG位点。其中CPG(-1556)、CPG(-1483)、CPG(-1479)的甲基化率以及10个CPG位点的总甲基化率在RAU组［分别为（32.0±19.9）%、（53.0±13.4）%、（46.0±19.8）%及（39.3±12.4）%］均显著高于健康对照组［分别为（20.0±3.2）%、（35.5±12.3）%、（28.0±14.4）%及（32.6±5.8）%］（P<0.05）。IL-4 mRNA在RAU组患者外周血中的相对表达量（1.0±0.1）显著低于健康对照组（1.5±0.2）（P<0.01）。RAU组IL-4基因启动子总甲基化率与IL-4 mRNA相对表达量之间存在显著负相关关系（r=-0.494，P<0.05）。在多因素分析中，RAU组吸烟、维生素B12、叶酸与IL-4基因启动子总甲基化率显著相关（P<0.01）。 结论： RAU患者IL-4基因启动子的高甲基化可能与IL-4基因转录的降低有关；维生素B12、叶酸和吸烟对RAU患者外周血中IL-4基因甲基化水平可能有影响。.
The foreign Bt gene was transferred into protoplasts of soybean using PEG and PLO methods, respectively. The result indicated that the transformation frequency of PLO method was about 0.1% higher than PEG method. The PCR and Southern blotting analysis of the regeneration plants confirmed the integration of foreign gene into the genome of soybean.
Chemotherapy resistance is a major limiting factor in the cure of patients with laryngeal squamous cell carcinoma (LSCC). Lymphocyte antigen 6 superfamily member D (Ly6D) is highly expressed in various tumors, but its role and underlying molecular mechanisms in chemoresistance of LSCC cells remains largely unclear. In this study, we reveal that overexpression of Ly6D facilitates LSCC cell chemoresistance, while Ly6D silencing abolishes this phenotype. Moreover, bioinformatics analysis, PCR array, and functional analysis confirmed that activation of the Wnt/β-catenin pathway contributes to Ly6D-mediated chemoresistance. The genetic and pharmacological inhibition of β-catenin compromises chemoresistance mediated by Ly6D overexpression. Mechanistically, Ly6D overexpression significantly attenuates the expression of miR-509-5p, thereby unleashing its target gene CTNNB1 to activate Wnt/β-catenin pathway and ultimately promote chemoresistance. In contrast, Ly6D augmenting β-catenin-mediated chemoresistance in LSCC cells were reversed by ectopic expression of miR-509-5p. Furthermore, ectopic expression of miR-509-5p markedly repressed the two other targets, MDM2 and FOXM1. Taken together, these data not only reveal the key role of Ly6D/miR-509-5p/β-catenin in chemotherapy resistance, but also provide a new strategy for the clinical treatment of refractory LSCC.
0bjective To investigate the association of methylenetetrahydrofolate reductase polymorphisms with non-syndromic cleft lip with or without cleft palate (NSCL/P)Chinese population.Methods Genotyping of rs1801133 was conducted by PCR-based restriction fragment length polymorphism (RFLP) analysis in both 168 patients and the 192 controls.The χ2 goodness-of-fit test was applied to test whether or not the genotypic distributions of rs1801133 would be in Hardy-Weinberg equilibrium.The UNPHASED program was used to perform χ2 test for the associations.Results The genotypic distribution of C677T was not deviated from the Hardy-Weinberg equilibrium.The allele frequencies were significantly different between patients with NSCL/P and healthy individuals(P0.05).The frequency of T alleles in the patients was lower than that of C alleles compared with the healthy individuals.Conclusion The polymorphism of MTHFR C677T is associated with NSCL/P Chinese population and may play a role in the occurrence of this disease.
Objective:To investigate the oropharyngeal function recovery of oropharyngeal squamous cell carcinoma repaired by anterolateral thigh flap and forearm flap. Methods:Retrospective study between September 2016 and September 2020 complete line 37 cases of oropharyngeal cancer postoperative soft tissue defect of femoral anterolateral flap or forearm flap to repair the patient data, in which 22 cases, using the forearm skin flap to repair 15 cases with femoral anterolateral flap, flap survival rate of preparation, compare the two groups, the incidence of vascular crisis, The functions of swallowing, speech, and velopharyngeal closure were evaluated. Results:21 cases of forearm flaps survived, and 1 case had vascular crisis. After surgical exploration, the contralateral forearm flaps survived transplantation. Fourteen anterolateral femoral flaps survived, 2 flaps had vascular crisis, and 1 flap survived after thrombus removal. The other one was repaired with pectoralis major myocutaneous flap for necrosis. Swallowing, speech, palatopharyngeal closure and other functions of the patients after the two kinds of flap repair had higher satisfaction. Conclusion:Forearm flap and anterolateral thigh flap are good choices for soft tissue defect after oropharyngeal cancer, Must act according to the special details choice appropriate therapeutic schedule.目的：探讨股前外侧皮瓣与前臂皮瓣修复口咽鳞状细胞癌术后缺损的口咽功能恢复情况。 方法：回顾性研究2016年9月—2020年9月在山西医科大学第一医院口腔科完成的37例口咽癌术后软组织缺损行股前外侧皮瓣或前臂皮瓣修复的患者资料，其中22例采用前臂皮瓣修复，15例采用股前外侧皮瓣修复，比较2组皮瓣制备成活率、血管危象发生率，并评估患者术后的吞咽、言语、腭咽闭合等功能。 结果：21例前臂皮瓣成活，1例出现血管危象，手术探查后未能成活，取对侧前臂皮瓣移植后成活。14例股前外侧皮瓣成活，2例皮瓣出现血管危象，其中1例手术探查去除血栓后皮瓣成活，另1例皮瓣坏死，改为胸大肌皮瓣修复。2种皮瓣修复术后患者的吞咽、言语、腭咽闭合等功能均有较高的满意度。 结论：对于口咽癌术后的软组织缺损，前臂皮瓣或股前外侧皮瓣可达到良好的功能修复，需根据具体情况选择。.
Objective To investigate the relevance between smoking and peri-implantitis by analysing the clinical results of patients with Smoking and non-smoking.Methods 78 dental-implants of 35 patients with smoking were selected as the test group and 76 dental implants of 43 no-smoking patients were selected as the control group,three years after loading,the clinical indexes including retained rate,mSBI,PD and MBL were scored in a standard manner and compared.Results There were no significant difference in the mSBI,PD,MBL and RR between smoking group and no-smoking group(P0.05).Conclusions There were no correlation between smoking and peri-implantitis.
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