A new jujubogenin saponin was isolated from the stems of Colubrina retusa and identified as jujubogenin 3-O-α-l-arabinofuranosyl (1→2)-[3-O-(trans)-p-coumaroyl-β-d-glucopyranosyl (1→3)]-α-l-arabinopyranoside (4) on the basis of chemical and spectroscopic data. The antimycobacterial activity expressed as minimum inhibitory concentration (MIC) for compound 4 was 10 μg/mL.