Jiangman He

University of California, Riverside

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Marcella Teixeira

Washington State University

Isgouhi Kaloshian

University of California, Riverside

Meng Chen

Hunan Agricultural University

Juan Du

University of California, Riverside

Jingzhe Guo

University of California, Riverside

Yongjian Qiu

University of Mississippi

Zhenbiao Yang

Shenzhen University

Amy Litt

University of California, Riverside

Xiaowei Zhang

Chinese Academy of Sciences

Olenka Castaneda

Chaffey College

Cooperative Institutions

University of California, Riverside

Chinese Academy of Sciences

University of Chinese Academy of Sciences

Center for Excellence in Molecular Plant Sciences

Fujian Agriculture and Forestry University

South China Agricultural University

Shanghai Institutes for Biological Sciences

Ministry of Agriculture and Rural Affairs

Nanjing Agricultural University

Yale University

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A LysM Receptor Heteromer Mediates Perception of Arbuscular Mycorrhizal Symbiotic Signal in Rice

Molecular Plant (2019)

Jiangman He Chi Zhang Huiling Dai Huan Liu Xiaowei Zhang

Medicago truncatula

Lotus japonicus

Nod factor

Genetically modified rice

10.1016/j.molp.2019.10.015

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Citations (139)

Comparative expression profiling reveals gene functions in female meiosis and gametophyte development in Arabidopsis

The Plant Journal (2014)

Lihua Zhao Jiangman He Hanyang Cai Haiyan Lin Yanqiang Li

Summary Megasporogenesis is essential for female fertility, and requires the accomplishment of meiosis and the formation of functional megaspores. The inaccessibility and low abundance of female meiocytes make it particularly difficult to elucidate the molecular basis underlying megasporogenesis. We used high‐throughput tag‐sequencing analysis to identify genes expressed in female meiocytes ( FM s) by comparing gene expression profiles from wild‐type ovules undergoing megasporogenesis with those from the spl mutant ovules, which lack megasporogenesis. A total of 862 genes were identified as FM s, with levels that are consistently reduced in spl ovules in two biological replicates. Fluorescence‐assisted cell sorting followed by RNA ‐seq analysis of DMC 1: GFP ‐labeled female meiocytes confirmed that 90% of the FM s are indeed detected in the female meiocyte protoplast profiling. We performed reverse genetic analysis of 120 candidate genes and identified four FM genes with a function in female meiosis progression in Arabidopsis. We further revealed that KLU , a putative cytochrome P450 monooxygenase, is involved in chromosome pairing during female meiosis, most likely by affecting the normal expression pattern of DMC 1 in ovules during female meiosis. Our studies provide valuable information for functional genomic analyses of plant germline development as well as insights into meiosis.

Meiocyte

Megaspore

10.1111/tpj.12657

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Citations (46)

Direct photoresponsive inhibition of a p53-like transcription activation domain in PIF3 by Arabidopsis phytochrome B

bioRxiv (Cold Spring Harbor Laboratory) (2021)

Chan Yul Yoo Qing Sang Jiangman He Yongjian Qiu Lingyun Long

ABSTRACT Phytochrome B (PHYB) triggers diverse light responses in Arabidopsis by binding to a group of antagonistically acting PHYTOCHROME-INTERACTING transcription FACTORs (PIFs) to promote PIF degradation, consequently downregulating PIF target genes. However, whether PHYB directly controls the transactivation activity of PIFs remains ambiguous. Here we show that the prototypic PIF, PIF3, possesses a p53-like transcription activation domain (TAD) consisting of a sequence-specific, hydrophobic activator motif surrounded by acidic residues. A PIF3mTAD mutant in which the activator motif is replaced with alanines fails to activate PIF3 target genes in Arabidopsis in dark, light, and shade conditions, validating the in vivo functions of the PIF3 TAD. Intriguingly, binding of PHYB’s N-terminal photosensory module to the PHYB-binding site adjacent to the TAD inhibits its transactivation activity. These results unveil a photoresponsive transcriptional switching mechanism in which photoactivated PHYB directly masks the transactivation activity of PIF3. Our study also suggests the unexpected conservation of sequence-specific TADs between the animal and plant kingdoms.

Phytochrome

Transcription

Sequence motif

10.1101/2021.05.11.443493

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Citations (0)

Additional file 1: of Classification and phylogenetic analyses of the Arabidopsis and tomato G-type lectin receptor kinases

Figshare (2018)

Marcella Teixeira Alex Rajewski Jiangman He Olenka Castaneda Amy Litt

Members of the columbine G-lectins and their domains. (XLSX 57Â kb)

C-type lectin

10.6084/m9.figshare.6109223

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A circadian clock output functions independently of phyB to sustain daytime PIF3 degradation

Proceedings of the National Academy of Sciences (2024)

Wei Liu Harper Lowrey Anxu Xu Chun Chung Leung Christopher Adamchek

The circadian clock is an endogenous oscillator, and its importance lies in its ability to impart rhythmicity on downstream biological processes, or outputs. Our knowledge of output regulation, however, is often limited to an understanding of transcriptional connections between the clock and outputs. For instance, the clock is linked to plant growth through the gating of photoreceptors via rhythmic transcription of the nodal growth regulators, PHYTOCHROME-INTERACTING FACTORs (PIFs), but the clock's role in PIF protein stability is less clear. Here, we identified a clock-regulated, F-box type E3 ubiquitin ligase, CLOCK-REGULATED F-BOX WITH A LONG HYPOCOTYL 1 (CFH1), that specifically interacts with and degrades PIF3 during the daytime. Additionally, genetic evidence indicates that CFH1 functions primarily in monochromatic red light, yet CFH1 confers PIF3 degradation independent of the prominent red-light photoreceptor phytochrome B (phyB). This work reveals a clock-mediated growth regulation mechanism in which circadian expression of CFH1 promotes sustained, daytime PIF3 degradation in parallel with phyB signaling.

Phytochrome

Oscillating gene

10.1073/pnas.2408322121

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Citations (1)

CamelliA-based simultaneous imaging of Ca²⁺ dynamics in subcellular compartments

bioRxiv (Cold Spring Harbor Laboratory) (2021)

Jingzhe Guo Jiangman He Katayoon Dehesh Zhenbiao Yang

Abstract As a universal second messenger, calcium (Ca 2+ ) transmits specific cellular signals via a spatiotemporal signature generated from its extracellular source and internal stores. Our knowledge of the mechanisms underlying generation of a Ca 2+ signature is hampered by limited tools enabling simultaneous monitoring of the dynamics of Ca 2+ levels in multiple subcellular compartments. To overcome the limitation and to further improve spatiotemporal resolutions, here we have assembled a molecular toolset (the CamelliA lines) in Arabidopsis that enables simultaneous and high-resolution monitoring of Ca 2+ dynamics in multiple subcellular compartments through imaging analyses of different single-colored GECIs (Genetically Encoded Calcium Indicators). Indeed, the uncovering of the previously unrecognized Ca 2+ signatures in three types of Arabidopsis cells in response to internal and external cues is a testimony to the wide applicability of the newly generated toolset for elucidating the subcellular sources contributing to the Ca 2+ signatures in plants. One sentence summary A toolset for simultaneous imaging of Ca 2+ dynamics in subcellular compartments has uncovered unrecognized Ca 2+ signatures in Arabidopsis cells in response to developmental and external cues.

Dynamics

Live cell imaging

10.1101/2021.05.31.446496

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Direct photoresponsive inhibition of a p53-like transcription activation domain in PIF3 by Arabidopsis phytochrome B

Nature Communications (2021)

Chan Yul Yoo Jiangman He Qing Sang Yongjian Qiu Lingyun Long

Photoactivated phytochrome B (PHYB) binds to antagonistically acting PHYTOCHROME-INTERACTING transcription FACTORs (PIFs) to regulate hundreds of light responsive genes in Arabidopsis by promoting PIF degradation. However, whether PHYB directly controls the transactivation activity of PIFs remains ambiguous. Here we show that the prototypic PIF, PIF3, possesses a p53-like transcription activation domain (AD) consisting of a hydrophobic activator motif flanked by acidic residues. A PIF3mAD mutant, in which the activator motif is replaced with alanines, fails to activate PIF3 target genes in Arabidopsis, validating the functions of the PIF3 AD in vivo. Intriguingly, the N-terminal photosensory module of PHYB binds immediately adjacent to the PIF3 AD to repress PIF3's transactivation activity, demonstrating a novel PHYB signaling mechanism through direct interference of the transactivation activity of PIF3. Our findings indicate that PHYB, likely also PHYA, controls the stability and activity of PIFs via structurally separable dual signaling mechanisms.

Phytochrome

Transcription

10.1038/s41467-021-25909-5

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Citations (28)

Classification and phylogenetic analyses of the Arabidopsis and tomato G-type lectin receptor kinases

BMC Genomics (2018)

Marcella Teixeira Alex Rajewski Jiangman He Olenka Castaneda Amy Litt

Pathogen perception by plants is mediated by plasma membrane-localized immune receptors that have varied extracellular domains. Lectin receptor kinases (LecRKs) are among these receptors and are subdivided into 3 classes, C-type LecRKs (C-LecRKs), L-type LecRKs (L-LecRKs) and G-type LecRKs (G-LecRKs). While C-LecRKs are represented by one or two members in all plant species investigated and have unknown functions, L-LecRKs have been characterized in a few plant species and have been shown to play roles in plant defense against pathogens. Whereas Arabidopsis G-LecRKs have been characterized, this family of LecRKs has not been studied in tomato.This investigation updates the current characterization of Arabidopsis G-LecRKs and characterizes the tomato G-LecRKs, using LecRKs from the monocot rice and the basal eudicot columbine to establish a basis for comparisons between the two core eudicots. Additionally, revisiting parameters established for Arabidopsis nomenclature for LecRKs is suggested for both Arabidopsis and tomato. Moreover, using phylogenetic analysis, we show the relationship among and between members of G-LecRKs from all three eudicot plant species. Furthermore, investigating presence of motifs in G-LecRKs we identified conserved motifs among members of G-LecRKs in tomato and Arabidopsis, with five present in at least 30 of the 38 Arabidopsis members and in at least 45 of the 73 tomato members.This work characterized tomato G-LecRKs and added members to the currently characterized Arabidopsis G-LecRKs. Additionally, protein sequence analysis showed an expansion of this family in tomato as compared to Arabidopsis, and the existence of conserved common motifs in the two plant species as well as conserved species-specific motifs.

10.1186/s12864-018-4606-0

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Citations (34)

A G-lectin Receptor Kinase is a Negative Regulator of Arabidopsis Immunity Against Root-Knot Nematode Meloidogyne incognita

bioRxiv (Cold Spring Harbor Laboratory) (2021)

Dongmei Zhou Damaris Godinez-Vidal Jiangman He Marcella Teixeira Jingzhe Guo

Abstract Root-knot nematodes ( Meloidogyne spp., RKN) are responsible for extensive crop losses worldwide. For infection, they penetrate plant roots, migrate between plant cells, and establish feeding sites, known as giant cells, in the root pericycle. Previously, we found that nematode perception and early plant responses were similar to those for microbial pathogens and require the BAK1 co-receptor in Arabidopsis thaliana and tomato. To identify additional receptors involved in this process, we implemented a reverse genetic screen for resistance or sensitivity to RKN using Arabidopsis T-DNA alleles of genes encoding transmembrane receptor-like kinases. This screen identified a pair of allelic mutations with enhanced resistance to RKN in a gene we named ENHANCED RESISTANCE TO NEMATODES 1 (ERN1). ERN1 encodes a G-type lectin receptor kinase (G-LecRK) with a single pass transmembrane domain. Further characterization showed that ern1 mutants displayed stronger activation of MAP kinases, elevated levels of the defense marker MYB51 , and enhanced H 2 0 2 accumulation in roots upon RKN elicitor treatments. Elevated MYB51 expression and ROS burst were also observed in leaves of ern1 mutants upon flg22 treatment. Complementation of ern1.1 with 35S- or native promotor-driven ERN1 rescued the RKN infection and enhanced defense phenotypes. Taken together, our results indicate that ERN1 is an important negative regulator of immunity. One sentence summary A plasma membrane localized G-lectin receptor kinase acts as a negative immune regulator by interfering with defense responses activated by nematode and microbial elicitors.

Root-knot nematode

10.1101/2021.09.07.459316

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Citations (3)

Photobody formation spatially segregates two opposing phytochrome B signaling actions of PIF5 degradation and stabilization

Nature Communications (2024)

R KIM D. Fan Jiangman He Keunhwa Kim Juan Du

Abstract Photoactivation of the plant photoreceptor and thermosensor phytochrome B (PHYB) triggers its condensation into subnuclear membraneless organelles named photobodies (PBs). However, the function of PBs in PHYB signaling remains frustratingly elusive. Here, we found that PHYB recruits PHYTOCHROME-INTERACTING FACTOR 5 (PIF5) to PBs. Surprisingly, PHYB exerts opposing roles in degrading and stabilizing PIF5. Perturbing PB size by overproducing PHYB provoked a biphasic PIF5 response: while a moderate increase in PHYB enhanced PIF5 degradation, further elevating the PHYB level stabilized PIF5 by retaining more of it in enlarged PBs. Conversely, reducing PB size by dim light, which enhanced PB dynamics and nucleoplasmic PHYB and PIF5, switched the balance towards PIF5 degradation. Together, these results reveal that PB formation spatially segregates two antagonistic PHYB signaling actions – PIF5 stabilization in PBs and PIF5 degradation in the surrounding nucleoplasm – which could enable an environmentally sensitive, counterbalancing mechanism to titrate nucleoplasmic PIF5 and environmental responses.

Degradation

Phytochrome

Organelle

10.1038/s41467-024-47790-8

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Citations (3)