Georgina Hernández

Universidad Nacional Autónoma de México

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Mario Ramı́rez

Universidad Nacional Autónoma de México

L. Iñiguez

Centre for Genomic Regulation

Oswaldo Valdés‐López

Universidad Nacional Autónoma de México

Carroll P. Vance

University of Minnesota

Alfonso Leija

Universidad Nacional Autónoma de México

Lourdes Girard

Universidad Nacional Autónoma de México

Sara Fuentes

Open Geospatial Consortium

Miguel Lara

Universidad Nacional Autónoma de México

Francisco Bolívar

Universidad Nacional Autónoma de México

Damien Formey

Universidad Nacional Autónoma de México

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Universidad Nacional Autónoma de México

150

Universidad Autónoma del Estado de Morelos

University of Minnesota

United States Department of Agriculture

Instituto Politécnico Nacional

Agricultural Research Service

Centre National de la Recherche Scientifique

Noble Research Institute

Center for Genomic Science

Instituto de Biotecnología de León

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TERNA PARA DIRIGIR EL CENTRO DE INVESTIGACIÓN SOBRE FIJACIÓN DE NITRÓGENO

Gaceta UNAM (1990-1999) (1997)

Consejo Técnico De La Investigación Científica Georgina Hernández Esperanza Martínez‐Romero David René Romero

EL CONSEJO TECNICO DE LA INVESTIGACION CIENTIFICA APROBO EN SU SESION ORDINARIA DEL 13 DE MARZO LA TERNA PARA LA DIRECCION DEL CENTRO DE INVESTIGACION SOBRE FIJACION DE NITROGENO PARA EL PERIODO 1997-2001. LOS CANDIDATOS QUE LA INTEGRAN SON: GEORGINA HERNANDEZ DELGADO, ESPERANZA MARTINEZ ROMERO Y DAVID RENE ROMERO CAMARENA. SE ANOTAN DATOS CURRICULARES DE LA TERNA.

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Transgenic tobacco plants that overexpress alfalfa NADH‐glutamate synthase have higher carbon and nitrogen content

Journal of Experimental Botany (2001)

Svetlana Chichkova Jesús Arellano Carroll P. Vance Georgina Hernández

This work reports the characterization of transgenic tobacco (Nicotiana tabacum L.) plants that constitutively overexpress NADH‐GOGAT. Three independent transformants, designated GOS10, GOS13 and GOS19 (for GOGAT sense), with stable integration of the chimeric alfalfa NADH‐GOGAT gene fused to the CaMV 35S promoter were studied. The transgene was stably integrated and inherited by the progeny. In these GOS lines, the expression of NADH‐GOGAT mRNA and protein was detected at low levels in roots and leaves, while the expression of the host tobacco NADH‐GOGAT gene was nearly undetectable. The roots of GOS lines showed an elevated (15–40%) enzyme activity as compared to control plants. When GOS plants were grown under greenhouse conditions and fed with either nitrate or ammonium as the sole nitrogen source, they showed higher total carbon and nitrogen content in shoots and increased shoot dry weight when plants were entering into the flowering stage, as compared to control plants. The observed phenotype of GOS plants was interpreted as reflecting a higher capacity to assimilate nitrogen due to a higher NADH‐GOGAT activity.

Glutamate synthase

10.1093/jexbot/52.364.2079

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Identification of miRNAs linked to peanut nodule functional processes

Journal of Biosciences (2020)

María Soledad Figueredo Damien Formey Johan Rodríguez Fernando Ibáñez Georgina Hernández

Arachis hypogaea

10.1007/s12038-020-00034-5

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Regulation of glutamine synthesis by glycine and serine in Neurospora crassa

Journal of Bacteriology (1986)

Georgina Hernández Y. Mora Jaime Font de Mora

The biosynthetic activities of the polypeptide subunits alpha and beta of glutamine synthetase (GS) were inhibited in vitro by glycine and serine. These amino acids inhibited the growth of a mutant strain with partial GS activity when grown on glutamate as the nitrogen source and also blocked the synthesis of the glutamine in vivo, thus demonstrating the inhibitory effect on GS activity in vivo. Glycine and serine lowered the intracellular glutamine pool and regulated GS beta synthesis. A preferential induction of synthesis of the GS beta polypeptide was observed when either of these amino acids was present in the medium. On this basis, we obtained a glycine-sensitive mutant which showed a structural alteration of the GS beta polypeptide. The double regulatory effect of either glycine or serine on glutamine synthesis may be considered an example of the regulation of glutamine synthesis by alpha-amino nitrogen. It may be a mechanism that regulates the assimilation of ammonium into glutamate versus glutamine.

Glycine cleavage system

Amino acid synthesis

10.1128/jb.165.1.133-138.1986

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MicroRNAs as regulators in plant metal toxicity response

Frontiers in Plant Science (2012)

Ana Belén Mendoza-Soto Federico Sánchez Georgina Hernández

Metal toxicity is a major stress affecting crop production. This includes metals that are essential for plants (copper, iron, zinc, manganese), and non-essential metals (cadmium, aluminum, cobalt, mercury). A primary common effect of high concentrations of metal such as aluminum, copper, cadmium, or mercury is root growth inhibition. Metal toxicity triggers the accumulation of reactive oxygen species leading to damage of lipids, proteins, and DNA. The plants response to metal toxicity involves several biological processes that require fine and precise regulation at transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) are 21 nucleotide non-coding RNAs that regulate gene expression at the post-transcriptional level. A miRNA, incorporated into a RNA-induced silencing complex, promotes cleavage of its target mRNA that is recognized by an almost perfect base complementarity. In plants, miRNA regulation is involved in development and also in biotic and abiotic stress responses. We review novel advances in identifying miRNAs related to metal toxicity responses and their potential role according to their targets. Most of the targets for plant metal-responsive miRNAs are transcription factors. Information about metal-responsive miRNAs in different plants points to important regulatory roles of miR319, miR390, miR393, and miR398. The target of miR319 is the TCP transcription factor, implicated in growth control. miR390 exerts its action through the biogenesis of trans-acting small interference RNAs that, in turn, regulate auxin responsive factors. miR393 targets the auxin receptors TIR1/AFBs and a bHLH transcription factor. Increasing evidence points to the crucial role of miR398 and its targets Cu/Zn superoxide dismutases in the control of the oxidative stress generated after high copper or iron exposure.

10.3389/fpls.2012.00105

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Root exuded nod-gene inducing signals limit the nodulation capacity of different alfalfa varieties with Rhizobium meliloti

Plant Cell Reports (1995)

Georgina Hernández Mario Ramı́rez Ramón Suárez‐Rodríguez Sara Fuentes

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Strain (injury)

Limiting

10.1007/bf00232726

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Essential role of MYB transcription factor: PvPHR1 and microRNA: PvmiR399 in phosphorus‐deficiency signalling in common bean roots

Plant Cell & Environment (2008)

Oswaldo Valdés‐López Catalina Arenas‐Huertero Mario Ramı́rez Lourdes Girard Federico Sánchez

ABSTRACT Phosphorus (P), an essential element for plants, is one of the most limiting nutrients for plant growth. A few transcription factor (TF) genes involved in P‐starvation signalling have been characterized for Arabidopsis thaliana and rice. Crop production of common bean ( Phaseolus vulgaris L.), the most important legume for human consumption, is often limited by low P in the soil. Despite its agronomic importance, nothing is known about transcriptional regulation in P‐deficient bean plants. We functionally characterized the P‐deficiency‐induced MYB TF TC3604 (Dana Farber Cancer Institute, Common Bean Gene Index v.2.0), ortholog to AtPHR1 ( PvPHR1 ). For its study, we applied RNAi technology in bean composite plants. PvPHR1 is a positive regulator of genes implicated in P transport, remobilization and homeostasis. Although there are no reports on the regulatory roles of microRNAs (miRNA) in bean, we demonstrated that PvmiR399 is an essential component of the PvPHR1 signalling pathway. The analysis of DICER‐like1 ( PvDCL1 ) silenced bean composite plants suppressed for accumulation of PvmiR399 and other miRNAs suggested that miR399 is a negative regulator of the ubiquitin E2 conjugase: PvPHO2 expression. Our results set the basis for understanding the signalling for P‐starvation responses in common bean and may contribute to crop improvement.

MYB

NFAT

10.1111/j.1365-3040.2008.01883.x

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Citations (182)

Additional file 10: of Identification and analysis of alternative splicing events in Phaseolus vulgaris and Glycine max

Figshare (2017)

L. Iñiguez Mario Ramı́rez W. Brad Barbazuk Georgina Hernández

P. vulgaris and homologous G. max genes selected for RT-PCR analysis. (XLSX 9 kb)

Identification

10.6084/m9.figshare.c.3860626_d1

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Highlights of nitrogen fixation research. Proceedings of the Sixteenth North American Conference on Symbiotic Nitrogen Fixation, held in Cancun, Mexico, February 1-6, 1998.

Esperanza Martínez‐Romero Georgina Hernández

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Common bean (Phaseolus vulgarisL.) PvTIFY orchestrates global changes in transcript profile response to jasmonate and phosphorus deficiency

BMC Plant Biology (2013)

Rosaura Aparicio‐Fabre Gabriel Guillén Montserrat Loredo Jesús Arellano Oswaldo Valdés‐López

Abstract Background TIFY is a large plant-specific transcription factor gene family. A subgroup of TIFY genes named JAZ (Jasmonate-ZIM domain) has been identified as repressors of jasmonate (JA)-regulated transcription in Arabidopsis and other plants. JA signaling is involved in many aspects of plant growth/development and in defense responses to biotic and abiotic stresses. Here, we identified the TIFY genes (designated PvTIFY ) from the legume common bean ( Phaseolus vulgaris) and functionally characterized PvTIFY10C as a transcriptional regulator. Results Nineteen genes from the PvTIFY gene family were identified through whole-genome sequence analysis. Most of these were induced upon methyl-JA elicitation. We selected PvTIFY10C as a representative JA-responsive PvTIFY gene for further functional analysis. Transcriptome analysis via microarray hybridization using the newly designed Bean Custom Array 90 K was performed on transgenic roots of composite plants with modulated (RNAi-silencing or over-expression) PvTIFY10C gene expression. Data were interpreted using Gene Ontology and MapMan adapted to common bean. Microarray differential gene expression data were validated by real-time qRT-PCR expression analysis. Comparative global gene expression analysis revealed opposite regulatory changes in processes such as RNA and protein regulation, stress responses and metabolism in PvTIFY10C silenced vs. over-expressing roots. These data point to transcript reprogramming (mainly repression) orchestrated by PvTIFY10C . In addition, we found that several PvTIFY genes, as well as genes from the JA biosynthetic pathway, responded to P-deficiency. Relevant P-responsive genes that participate in carbon metabolic pathways, cell wall synthesis, lipid metabolism, transport, DNA, RNA and protein regulation, and signaling were oppositely-regulated in control vs. PvTIFY10C -silenced roots of composite plants under P-stress. These data indicate that PvTIFY10C regulates, directly or indirectly, the expression of some P-responsive genes; this process could be mediated by JA-signaling. Conclusion Our work contributes to the functional characterization of PvTIFY transcriptional regulators in common bean, an agronomically important legume. Members from the large PvTIFY gene family are important global transcriptional regulators that could participate as repressors in the JA signaling pathway. In addition, we propose that the JA-signaling pathway involving PvTIFY genes might play a role in regulating the plant response/adaptation to P-starvation.

Jasmonate

Methyl jasmonate

WRKY protein domain

10.1186/1471-2229-13-26

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Citations (41)