Karolina Nowak

Wayne State University

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Ewa Jabłońska

Medical University of Białystok

Wioletta Ratajczak–Wrona

Medical University of Białystok

Marzena Garley

Medical University of Białystok

Ute Gschwandtner

University Hospital of Basel

Peter Fuhr

University Hospital of Basel

Andrea H. Meyer

University of Basel

Piotr Radziwon

Medical University of Białystok

Menorca Chaturvedi

National Centre for Disease Informatics and Research

Lucyna Papierska

Postgraduate School of Molecular Medicine

Anousha Hadinia

University Hospital of Basel

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Medical University of Białystok

University Hospital of Basel

University of Basel

Wayne State University

Medical University of Warsaw

Postgraduate School of Molecular Medicine

Uniwersytet Komisji Edukacji Narodowej w Krakowie

University of Białystok

Grochowski Hospital

University of Massachusetts Amherst

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Sex-dependent dysregulation of human neutrophil responses by bisphenol A

Research Square (Research Square) (2020)

Wioletta Ratajczak–Wrona Marzena Garley Małgorzata Rusak Karolina Nowak Jan Czerniecki

Abstract Background: In the present study, we aimed to investigate selected functions of human neutrophils exposed to bisphenol A (BPA) under in vitro conditions. As BPA is classified among xenoestrogens, we compared its action and effects with those of 17β-estradiol (E2). Methods: Chemotaxis of neutrophils was examined using the Boyden chamber. Their phagocytosis and nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase activity were assessed via Park’s method with latex beads and Park’s test with nitroblue tetrazolium . To assess the total concentration of nitric oxide (NO), the Griess reaction was utilized. Flow cytometry was used to assess the expression of cluster of differentiation (CD) antigens. The formation of neutrophil extracellular traps (NETs) was analyzed using a microscope (IN Cell Analyzer 2200 system). Expression of the investigated proteins was determined using Western blot. Results: The analysis of results obtained for both sexes demonstrated that after exposure to BPA, the chemotactic capacity of neutrophils was reduced. In the presence of BPA, the phagocytic activity was found to be elevated in the cells obtained from women and reduced in the cells from men. Following exposure to BPA, the percentage of neutrophils with CD14 and CD284 (TLR4) expression, as well as the percentage of cells forming NETs, was increased in the cells from both sexes. The stimulatory role of BPA and E2 in the activation of NADPH oxidase was observed only in female cells. On the other hand, no influence of E2 on the expression of CD14 and CD284, chemotaxis, phagocytosis, and the amount of NET-positive neutrophils was found for both sexes. The study further showed that BPA intensified NO production and iNOS expression in the cells of both sexes. In addition, intensified expression of all tested PI3K-Akt pathway proteins was observed in male neutrophils. Conclusions: The study demonstrated the influence of BPA on neutrophil functions associated with locomotion and pathogen elimination, which in turn may disturb the immune response of these cells in both women and men. Analysis of the obtained data showed that the effect of this xenoestrogen on the human neutrophils was more pronounced than E2.

Xenoestrogen

Neutrophil Extracellular Traps

10.21203/rs.3.rs-26406/v3

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Citations (1)

NETs biomarkers in saliva and serum OSCC patients: One hypothesis, two conclusions

Advances in Medical Sciences (2021)

Marzena Garley Dorota Dziemiańczyk-Pakieła Wioletta Ratajczak–Wrona Anna Pryczynicz Karolina Nowak

The actual role of neutrophils and neutrophil extracellular traps (NETs) in the course of cancer has not been clearly defined. The aim of this study was to determine the clinical usefulness of NETs biomarkers in saliva in confrontation with the blood serum and tumor tissue as a potential prognostic and therapeutic target in patients with oral squamous cell carcinoma (OSCC).Expression of myeloperoxidase (MPO), and histones H2A, H2B, H3 in the tumor tissue, was investigated using immunohistochemistry. The expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits: p47-phox, p67-phox (neutrophil cytosolic factor 2, NCF2) and panRac, as well as citrullinated histone H3 (CitH3) in peripheral blood neutrophil lysates, was assessed via Western blot. ELISA tests were employed to measure the concentrations of circulating free DNA (cfDNA) and MPO in saliva only, and NOX1, NCF2, DNASE1 in saliva and serum.Extracellular expression of MPO and histones was localized within tumor tissue. Significantly lower expression of p67-phox, panRac, and CitH3 was determined in OSCC patients. Considerably lower concentrations of NOX1, NCF2, and DNASE1 in the saliva samples of cancer patients were observed. However, the levels of NOX1, NCF2, and DNASE1 in the serum of patients with cancer were substantially higher.The results obtained from the saliva of cancer patients suggest an impairment of the immunological homeostasis within the oral cavity related to NET formation, the causes of which should be sought in deficient activation of NADPH oxidase.

Neutrophil Extracellular Traps

Neutrophil elastase

10.1016/j.advms.2021.12.004

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N-nitrosodimethylamine (NDMA) induced apoptosis dependent on Fas/FasL complex in human leukocytes

Human & Experimental Toxicology (2019)

Agnieszka Iwaniuk Kamil Grubczak Wioletta Ratajczak–Wrona Marzena Garley Karolina Nowak

To investigate the mechanism of apoptosis dependent on the Fas/FasL (Fas ligand) complex in the presence of N-nitrosodimethylamine (NDMA) in human leukocytes.Polymorphonuclear neutrophils (PMNs) and peripheral blood mononuclear cells (PBMCs) were isolated form whole blood by density centrifugation. The concentration of NDMA was assessed by cellular toxicity assay. Apoptotic cells were assessed with flow cytometry and the expression of pro- and antiapoptotic proteins was investigated by Western blotting in PMNs and PBMCs treated with NDMA and/or FasL.PMNs showed a higher ratio of apoptotic cells than PBMCs after exposure to NDMA and/or FasL. Enhanced apoptosis was related to the increased expression of proapoptotic proteins in neutrophils following exposure to either NDMA or FasL. In PBMCs, the relation was observed after exposure to FasL only. PMNs and PBMCs incubated with NDMA and FasL simultaneously demonstrated the highest increase in protein expression.NDMA shows a stronger proapoptotic effect with PMNs than with PBMCs. The Fas/FasL complex, along with other proapoptotic proteins of the receptor (Fas, FADD) and mitochondrial pathway (Noxa, Puma, Bim), plays a key role in the induction of neutrophil apoptosis. Synergic effects of NDMA and FasL which lead to higher induction of apoptosis in PMNs than in PBMCs indicates a multistage and varied regulation of apoptosis in different populations of leukocytes.

Fas ligand

FADD

N-Nitrosodimethylamine

10.1177/0960327119828198

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Urinary concentrations of phthalate and phthalate alternative metabolites and sperm DNA methylation: A multi-cohort and meta-analysis of men in preconception studies

Environment International (2024)

Karolina Nowak Oladele Oluwayiose Emily Houle DruAnne L. Maxwell Savni Sawant

Sperm Quality

10.1016/j.envint.2024.109049

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The promoting effect of neutrophil-derived BAFF molecule on the proliferation and life span of CAL-27 oral squamous carcinoma cells

Immunobiology (2022)

Ewa Jabłońska Agnieszka Iwaniuk Wioletta Ratajczak–Wrona Kamil Grubczak Dorota Dziemiańczyk-Pakieła

10.1016/j.imbio.2022.152247

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Citations (3)

Bindungsverhalten von Anti-ACE-Antikörpern am isoliert ventilierten und perfundierten humanen Lungenresektat

Atemwegs- und Lungenkrankheiten (2007)

Karolina Nowak Herbert Kölbel R. Metzger C Hanusch Stefan Post

10.5414/atp33343

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Cognitive Behavioral Group Therapy Reduces Stress and Improves the Quality of Life in Patients with Parkinson’s Disease

Frontiers in Psychology (2017)

Anousha Hadinia Andrea H. Meyer Viviane Bruegger Florian Hatz Karolina Nowak

Objective: The aim of this study is to compare a cognitive behavioral group therapy (CBT) with a health enhancement program (HEP) for stress reduction and the impact on quality of life (QoL) in patients with Parkinson's disease (PD). Method: Thirty patients with PD participated in the study: 16 received CBT including stress-reducing elements and 14 took part in a HEP. The two groups did not differ significantly in their baseline demographic characteristics. The patients in both groups underwent weekly sessions of 2 h duration for 9 weeks. The Parkinson's Disease Questionnaire with 39 items (PDQ-39), the Burden Questionnaire for Parkinson's Disease (translated from the original German: Belastungsfragebogen für Parkinsonpatienten (BELA) and the Disease-Related Questionnaire [Fragebogen zur krankheitsbezogenen Kommunikation (FKK)] were used for assessment. Ratings were completed at baseline and after 9 weeks (immediately after the last treatment session). Results: The patients in the CBT group achieved significantly better BELA, FKK and PDQ-39 scores (p < 0.05). Subscale analysis revealed that the scores on the BELA subscales "emotional well-being" and "somatic motor function" contributed significantly to stress reduction (p < 0.05). The FKK revealed significant improvement in social skills in the CBT group (p < 0.05). Conclusion: Cognitive Behavioral Group Therapy appears to be an effective way for patients with PD to lessen stress and improve their quality of life.

10.3389/fpsyg.2016.01975

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Citations (16)

Fine motor function skills in patients with Parkinson disease (PD) with and without mild cognitive impairment (MCI)

Parkinsonism & Related Disorders (2015)

Philippe Dahdal Andrea H. Meyer Menorca Chaturvedi Karolina Nowak Anne Dorothée Roesch

10.1016/j.parkreldis.2015.10.136

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Correlation of the EEG frequency with cognitive performance in Parkinson’s disease – six-months follow-up

Parkinsonism & Related Disorders (2015)

Vitalii Cozac Menorca Chaturvedi Florian Hatz Andrea H. Meyer Karolina Nowak