L. Gautam

Pennsylvania State University

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M. Sinha

Vyome Biosciences (India)

T.P. Singh

All India Institute of Medical Sciences

S. Sharma

National University of Malaysia

P.K. Shukla

University of Petroleum and Energy Studies

Punit Kaur

All India Institute of Medical Sciences

Pervinder Kaur

Punjab Agricultural University

Sujata Sharma

All India Institute of Medical Sciences

A. Bhushan

OQ (Oman)

Maria C. Bewley

Pennsylvania State University

S. Sharma

All India Institute of Medical Sciences

Cooperative Institutions

All India Institute of Medical Sciences

Pennsylvania State University

Council of Scientific and Industrial Research

Penn State Milton S. Hershey Medical Center

University of Delhi

Academy of Scientific and Innovative Research

Punjab Agricultural University

Chandigarh University

Amity University

Hershey (United States)

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Crystal structure of Peptidyl-tRNA hydrolase from Pseudomonas aeruginosa with 5-azacytidine at 1.89 Angstrom resolution

Akhilesh Kumar Singh L. Gautam M. Sinha A. Bhushan Preet Kamal

Angstrom

Hydrolase

10.2210/pdb4qd3/pdb

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Crystal Structure of C-lobe of Bovine lactoferrin Complexed with Lipoteichoic acid at 2.1 A Resolution

P.K. Shukla L. Gautam M. Sinha A. Bhushan Pervinder Kaur

Lipoteichoic acid

Lactoferrin

10.2210/pdb3sdf/pdb

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Crystal Structure of C-lobe of Bovine lactoferrin Complexed with Flurbiprofen at 1.58 A Resolution

P.K. Shukla L. Gautam Manoranjan Sinha Pervinder Kaur Shailendra Kumar Sharma

Flurbiprofen

Lactoferrin

10.2210/pdb4for/pdb

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Crystal Structure of C-lobe of Bovine lactoferrin Complexed with tolfenamic acid at 1.98 A Resolution

P.K. Shukla L. Gautam M. Sinha Pervinder Kaur S. Sharma

Lactoferrin

10.2210/pdb4g77/pdb

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Preparation and Antimicrobial Action of Three Tryptic Digested Functional Molecules of Bovine Lactoferrin

PLoS ONE (2014)

Nilisha Rastogi Nitish Nagpal Hammad Alam Sadanand Pandey L. Gautam

Lactoferrin is an 80 kDa bilobal, iron binding glycoprotein which is primarily antimicrobial in nature. The hydrolysis of lactoferrin by various proteases in the gut produces several functional fragments of lactoferrin which have varying molecular sizes and properties. Here, bovine lactoferrin has been hydrolyzed by trypsin, the major enzyme present in the gut, to produce three functional molecules of sizes approximately 21 kDa, 38 kDa and 45 kDa. The molecules have been purified using ion exchange and gel filtration chromatography and identified using N-terminal sequencing, which reveals that while the 21 kDa molecule corresponds to the N2 domain (21LF), the 38 kDa represents the whole C-lobe (38LF) and the 45 kDa is a portion of N1 domain of N-lobe attached to the C-lobe (45LF). The iron binding and release properties of 21LF, 38LF and 45LF have been studied and compared. The sequence and structure analysis of the portions of the excision sites of LF from various species have been done. The antibacterial properties of these three molecules against bacterial strains, Streptococcus pyogenes, Escherichia coli, Yersinia enterocolitica and Listeria monocytogenes were investigated. The antifungal action of the molecules was also evaluated against Candida albicans. This is the first report on the antimicrobial actions of the trypsin cleaved functional molecules of lactoferrin from any species.

Lactoferrin

10.1371/journal.pone.0090011

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Crystal Structure of C-lobe of Bovine lactoferrin Complexed with Phenyl-Propanolamine at 1.97 A Resolution

P.K. Shukla L. Gautam M. Sinha Punit Kaur S. Sharma

Lactoferrin

10.2210/pdb3u8q/pdb

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Crystal Structure of C-lobe of Bovine lactoferrin Complexed with celecoxib acid at 1.80 A Resolution

P.K. Shukla L. Gautam M. Sinha P. Kaur S. Sharma

Lactoferrin

10.2210/pdb4fim/pdb

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Current Perspectives in NSAID-Induced Gastropathy

Mediators of Inflammation (2013)

M. Sinha L. Gautam Prakash Shukla Punit Kaur Sujata Sharma

Nonsteroidal anti-inflammatory drugs (NSAIDs) are the most highly prescribed drugs in the world. Their analgesic, anti-inflammatory, and antipyretic actions may be beneficial; however, they are associated with severe side effects including gastrointestinal injury and peptic ulceration. Though several approaches for limiting these side effects have been adopted, like the use of COX-2 specific drugs, comedication of acid suppressants like proton pump inhibitors and prostaglandin analogs, these alternatives have limitations in terms of efficacy and side effects. In this paper, the mechanism of action of NSAIDs and their critical gastrointestinal complications have been reviewed. This paper also provides the information on different preventive measures prescribed to minimize such adverse effects and analyses the new suggested strategies for development of novel drugs to maintain the anti-inflammatory functions of NSAIDs along with effective gastrointestinal protection.

Antipyretic

Limiting

Antiinflammatory drug

10.1155/2013/258209

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Molecular architecture and domain arrangement of the placental malaria protein VAR2CSA suggests a model for receptor binding

bioRxiv (Cold Spring Harbor Laboratory) (2020)

Maria C. Bewley L. Gautam D. Channe Gowda John M. Flanagan

ABSTRACT VAR2CSA is the placental-malaria specific member of the antigenically variant Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family. It is expressed on the surface of Plasmodium falciparum infected host red blood cells and binds to specific chondroitin-4-sulfate (CSA) chains of the placental proteoglycan receptor. The functional ~310 kDa ectodomain of VAR2CSA is a multi-domain protein that requires a minimum 12-mer CSA molecule for specific, high affinity receptor binding. However, how these domains interact to create the receptor binding surface is not known, limiting efforts to exploit its potential as an effective vaccine or drug target. Using small angle X-ray scattering and single particle reconstruction from negative stained electron micrographs of the ectodomain and multidomain constructs, we have determined the structural architecture of VAR2CSA. The relative location of the domains creates two distinct pores that can each accommodate the 12-mer of CSA, suggesting a model for receptor binding. This model has important implications for understanding cytoadherence of IRBCs and potentially provides a starting point for developing novel strategies to prevent and/or treat placental malaria.

Ectodomain

10.1101/2020.04.16.045096

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Editorial Note on Cancer

Journal of Hematology and Thromboembolic Diseases (2021)

L. Gautam

Bone marrow or fringe blood undeveloped cell transplantation is utilized in the therapy of malignancy for two reasons. In the first place, transplantation licenses abuse of the lofty dose?response relationship found in certain tumors by permitting organization of dosages of foundational chemotherapy and radiotherapy that without transplantation would cause unsatisfactorily extreme or deadly myelosuppression. Second, transplantation of allogeneic marrow presents an antitumor impact, separate from the impacts of chemoradiotherapy. The first fruitful bone marrow transfers in quite a while were acted in the last part of the 1960s.

Chemoradiotherapy

10.35248/2329-8790.21.9.335

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