Nicolas Neyman

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André Bosly

CHU Dinant Godinne UCL Namur

François Mullier

Namur Research Institute for Life Sciences

Anne‐Catherine Lhoumeau

Institut Paoli-Calmettes

ÉA

Élisabeth André-Kerneïs

Hôpital Lyon Sud

Ludovic Lhermitte

Hôpital Necker-Enfants Malades

Victoria Raggeneau

Centre Hospitalier de Versailles

Véronique Salaün

Université de Caen Normandie

M. Georget

Centre Hospitalier Universitaire de Tours

ÉC

Édouard Cornet

Université de Caen Normandie

Michel Ticchioni

Hôpital l'Archet

Cooperative Institutions

Inserm

Centre National de la Recherche Scientifique

Assistance Publique – Hôpitaux de Paris

Université Paris Cité

CHU Dinant Godinne UCL Namur

UCLouvain

Hôpital Necker-Enfants Malades

Université Claude Bernard Lyon 1

Institut Necker Enfants Malades

University of Namur

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Apports de la cytologie et de la cytométrie en flux dans le diagnostic d'un lymphome T angio-immunoblastique (LTAI) avec envahissement sanguin et médullaire: cas clinique et revue de la littérature

Hématologie (2009)

François Mullier Yvan Cornet Nicolas Neyman A Dromelet Monique Delos

Auteur(s) : Francois Mullier, Yvan Cornet, Nicolas Neyman, Alex Dromelet, Monique Delos, Andre Bosly, Christian Chatelain, Bernard Chatelain UCL Mont-Godinne, Yvoir, Belgique Les envahissements a minima du sang et de la moelle par les lymphomes sont difficiles a mettre en evidence. Le cas rapporte ici illustre l’importance d’une interaction etroite entre la cytologie, l’histologie medullaire et la cytometrie en flux, et demontre l’interet d’une strategie « multicouleur [...]

10.1684/hma.2009.0365

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Standardization of Flow Cytometric Immunophenotyping for Hematological Malignancies: The FranceFlow Group Experience

Cytometry Part A (2019)

Françoise Solly Fanny Angelot‐Delettre Michel Ticchioni Franck Geneviève Hubert Rambaud

Abstract Flow cytometry is broadly used for the identification, characterization, and monitoring of hematological malignancies. However, the use of clinical flow cytometry is restricted by its lack of reproducibility across multiple centers. Since 2006, the EuroFlow consortium has been developing a standardized procedure detailing the whole process from instrument settings to data analysis. The FranceFlow group was created in 2010 with the intention to educate participating centers in France about the standardized instrument setting protocol (SOP) developed by the EuroFlow consortium and to organise several rounds of quality controls (QCs) in order to evaluate the feasibility of its application and its results. Here, we report the 5 year experience of the FranceFlow group and the results of the seven QCs of 23 instruments, involving up to 19 centers, in France and in Belgium. The FranceFlow group demonstrates that both the distribution and applicability of the SOP have been successful. Intercenter reproducibility was evaluated using both normal and pathological blood samples. Coefficients of variation (CVs) across the centers were <7% for the percentages of cell subsets and <30% for the median fluorescence intensities (MFIs) of the markers tested. Intracenter reproducibility provided similar results with CVs of <3% for the percentages of the majority of cell subsets, and CVs of <20% for the MFI values for the majority of markers. Altogether, the FranceFlow group show that the 19 participating labs might be considered as one unique laboratory with 23 identical flow cytometers able to reproduce identical results. Therefore, SOP significantly improves reproducibility of clinical flow in hematology and opens new avenues by providing a robust companion diagnostic tool for clinical trials in hematology. © 2019 International Society for Advancement of Cytometry

Immunophenotyping

10.1002/cyto.a.23844

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Improved registration form of test results of urethral and vaginal discharge microscopic examination in pregnant females

DOAJ (DOAJ: Directory of Open Access Journals) (2015)

Nicolas Neyman

The article is devoted to the analysis of difficulties in the performance of the microscopic analysis of vaginal smears of pregnant women that are connected with using current form of the analyses registration. This form doesn’t include such significant for pregnant women indices as erythrocyte level and degree of cleanliness of sheath discharge and also doesn’t register extended data on microbial flora and doesn’t provide data on the directional diagnosis. Also the form doesn’t correspond to the purposes of examination of women from post-natal wards. We proposed unitized form of the sheet of analysis registration to increase informativity of the analysis and to improve its quality.

Vaginal discharge

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