Daniel B. Ader

Walter Reed Army Institute of Research

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Dave Barvir

Walter Reed Army Institute of Research

Lara E. Gilmore

Walter Reed Army Institute of Research

John Bisbing

Walter Reed Army Institute of Research

Kristina K. Peachman

Walter Reed Army Institute of Research

Dupeh R. Palmer

Walter Reed Army Institute of Research

Wellington Sun

United States Food and Drug Administration

Vicky Gunther

Walter Reed Army Institute of Research

Mangala Rao

Walter Reed Army Institute of Research

Christina M. Celluzzi

American Association of Blood Banks

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Walter Reed Army Institute of Research

United States Food and Drug Administration

Henry M. Jackson Foundation

University of America

Catholic University of America

University of Maryland, Baltimore

Jackson Foundation

HJF Medical Research International

The University of Tokyo

Universidade de São Paulo

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Modulation of Dengue Virus Infection of Dendritic Cells byAedes aegyptiSaliva

Viral Immunology (2004)

Daniel B. Ader Christina M. Celluzzi John Bisbing Lara E. Gilmore Vicky Gunther

Dengue virus (DV) is a flavivirus carried by the Aedes aegypti mosquito that causes a spectrum of illnesses in the tropics, including dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. Dendritic cells (DCs) are professional antigen presenting cells recently shown to be permissive for DV, and implicated as the primary targets of initial DV infection. DV is transmitted to human host by infected mosquitoes during a blood meal, but it is currently unknown whether transmission is modified by vector saliva that is also deposited in the host's skin during feeding. Previous studies evaluated only the outcome of DV infection of DCs, and did not address the influence of mosquito saliva. To more fully characterize natural transmission of DV, we evaluated the effects of Ae. aegypti saliva on DV infection of human myeloid DCs. We found that saliva inhibited DV infection in DCs. Moreover, pre-sensitization of DCs with saliva, prior to DV infection, enhanced inhibition. In addition, enhanced production of IL-12p70 and TNF-α were detected in DV-infected DC cultures exposed to mosquito saliva. The proportion of dead cells was also significantly reduced in these cultures. These data contribute to the overall understanding of the natural pathogenesis of DV infection and suggest that there is a protective role for mosquito saliva that limits viral uptake by DCs.

Flavivirus

Blood meal

10.1089/0882824041310496

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Citations (53)