Purpose The purpose of this study was to detect the expression pattern of SPZ1 in glioma samples and to clarify its biological functions in the malignant progression of glioma. Our results provide a novel molecular target for glioma. Methods SPZ1 levels in 40 pairs of glioma and non-tumoral ones were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The differences in clinical indicators and prognosis between glioma patients expressing high and low levels of SPZ1 were compared. After knockdown of SPZ1 by transfection of sh-SPZ1, migratory and invasive abilities of A172 and U251 cells were examined by transwell migration and invasion assays. The interaction between SPZ1 and its target gene CXXC4 was finally explored by Western blot and dual-luciferase reporter assay. Results SPZ1 was upregulated in glioma tissues than non-tumoral ones, and the difference was statistically significant. Cell function experiments showed that knockdown of SPZ1 weakened the migratory and invasive abilities of A172 and U251 cells. CXXC4 was identified as the target gene binding to SPZ1. Knockdown of CXXC4 abolished the role of SPZ1 knockdown in inhibiting glioma progression. Conclusions SPZ1 stimulates glioma's malignant progression via targeting CXXC4.
The purpose of this study was to detect the expression pattern of SPZ1 in glioma samples and to clarify its biological functions in the malignant progression of glioma. Our results provide a novel molecular target for glioma.SPZ1 levels in 40 pairs of glioma and non-tumoral ones were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The differences in clinical indicators and prognosis between glioma patients expressing high and low levels of SPZ1 were compared. After knockdown of SPZ1 by transfection of sh-SPZ1, migratory and invasive abilities of A172 and U251 cells were examined by transwell migration and invasion assays. The interaction between SPZ1 and its target gene CXXC4 was finally explored by Western blot and dual-luciferase reporter assay.SPZ1 was upregulated in glioma tissues than non-tumoral ones, and the difference was statistically significant. Cell function experiments showed that knockdown of SPZ1 weakened the migratory and invasive abilities of A172 and U251 cells. CXXC4 was identified as the target gene binding to SPZ1. Knockdown of CXXC4 abolished the role of SPZ1 knockdown in inhibiting glioma progression.SPZ1 stimulates glioma's malignant progression via targeting CXXC4.
To identify risk factors associated with progressive hemorrhagic injury (PHI) in patients with isolated traumatic brain injury (TBI) and to develop prognostic models for predicting patient outcomes. A total of 137 patients with isolated TBI who underwent additional CT scans were included in the retrospective study. Single-factor analysis and multivariate logistic regression analysis were performed to identify significant risk factors associated with PHI development. Receiver operating characteristic (ROC) curve analysis was conducted to evaluate the diagnostic value of specific markers for predicting PHI. Single-factor analysis revealed significant differences between the PHI group (62 patients) and the non-PHI group (75 patients) in various factors, including gender, etiology, pupillary size and reflex, midline shift, associated brain contusion, D-dimer (D-D) levels, neutrophil-to-lymphocyte ratio (NLR), platelet count, blood glucose levels, and Glasgow Coma Scale (GCS) score. Multivariate logistic regression analysis identified NLR, blood glucose level, and GCS score as significant risk factors for PHI in isolated TBI patients, and also identified GCS score, NLR, platelet-to-lymphocyte ratio (PLR), and age as significant factors for predicting prognosis. ROC curve analysis showed that NLR had significant auxiliary diagnostic value for predicting PHI. NLR, blood glucose level, and GCS score are significant risk factors for PHI development in isolated TBI patients. The constructed prognostic model incorporating age, GCS score, NLR, and PLR offers valuable predictive capabilities for PHI patient outcome in isolated TBI cases.
Several recent studies have indicated the possibility of detecting dysregulated microRNAs (miRNAs) to diagnose nervous system cancer (NSC). Our study was conducted to explore the clinical applicability of miRNAs as potential ideal biomarkers for the diagnosis of NSC. For this meta-analysis, a systematic literature search was conducted in the Embase, Medline, Cochrane, Wangfang, and Sinomed databases. A standard quality tool-quality assessment of diagnostic accuracy studies was employed to assess the quality of the included studies. Specificity, sensitivity, diagnostic odds ratio (DOR), and area under curve (AUC) were pooled to assess overall test accuracy. In total, 25 studies from 7 articles, including 388 patients with NSC and 435 controls (healthy controls and patients with neurologic disorders), were included in this meta-analysis. For the studied miRNAs, the pooled sensitivity, specificity, and DOR for predicting NSC were 85% (95% confidence interval [CI] 80-89%), 85% (95% CI 80-89%), and 32 (95% CI 19-55), respectively. The pooled AUC for miRNAs identifying NSC was 0.92. In addition, results from subgroup analyses indicated that using miRNA panels yield a much better diagnostic accuracy when compared with using a particular miRNA. The current evidence suggests that miRNAs, especially miRNA panels on body fluids, may be suitable for use as diagnostic biomarkers for NSC patients. However, more prospective studies using larger cohorts should be conducted to confirm their degree of accuracy.
Objective To study the prophylaxis and control techniques of complications occurred in water-medium neuroendoscopic (or ventriculoscopic) operations, in an effort to build up our confidence in carrying out these kinds of operations. Methods Clinical records and follow-up data of 425 cases underwent ventriculoscopic operations, accomplished by the senior surgeon in the past 10 years, were investigated retrospectively with priority on the complications. Results 431 ventriculoscopic operations were carried out in 425 cases, 67 times complications were happened in 36 cases (8.5%), and all these patients were followed up for a mean time of 19.3 months. Complication rate was 18.0% in the early stage of neuroendoscopic practice (the first 50 cases), and 7.2% in the last stage (other 375 cases followed the first 50 cases). The complications were scalp incision problems (4.2% , including bad healing, cerebral spinal fluid leakage and infection), subdural hygroma (3.3%), intracranial hemorrhage (2.4%), intracranial infection (2.1%), cranial nerve injury (1.9%), hypothalamus injury (0.7%), cerebral infarction (0.5%), midbrain injury (0.5%), and thalamencephalon injury (0.2%). Twenty-four complications (5.6%) were temporary, and 12 (2.8%) were permanent. Eight (1.9%)of the 12 permanent cases were in serious consequences, and 2 (0.5%)of them died. Conclusion Ventriculoscopic operations are safe.Most complications are temporary, and the life-endangering complications are rare. In child less than 1 year old, the head incision must be managed seriously. In order to reduce the infectious rate, surgeons must exert every effort to make the scalp healed well. Post-operative subdural hygromas are likely to be happened in cases with serious hydrocephalus or large intracranial arachnoid cysts, associated surgical techniques must be stressed on during the operations.
Key words:
Neuroendoscopy; Postoperative complication; Hydrocephalus
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)
'%3e%3cpath fill='%23012169' d='M0 0v30h60V0z'/%3e%3cpath stroke='%23fff' stroke-width='6' d='m0 0 60 30m0-30L0 30'/%3e%3cpath stroke='%23C8102E' stroke-width='4' d='m0 0 60 30m0-30L0 30' clip-path='url(%23b)'/%3e%3cpath stroke='%23fff' stroke-width='10' d='M30 0v30M0 15h60'/%3e%3cpath stroke='%23C8102E' stroke-width='6' d='M30 0v30M0 15h60'/%3e%3c/g%3e%3c/svg%3e)