Juliana Moura Mendes

Universidad Nacional de Asunción

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Andrea Alejandra Arrúa Alvarenga

Universidad Nacional de Asunción

Cinthia Cazal

Universidad Nacional de Asunción

Danilo Fernández Ríos

Universidad Nacional de Asunción

Man Mohan Kohli

Guyra Paraguay

Edeltrudes de Oliveira Lima

Universidade Federal da Paraíba

Fillipe de Oliveira Pereira

Universidade Federal de Campina Grande

Francisco Ferreira

Universidad Nacional de Asunción

Pablo David Arrúa

Universidad Nacional de Asunción

Horacio D. Lopez-Nicora

The Ohio State University

Julio C. M. Iehisa

Universidad Nacional de Asunción

Cooperative Institutions

Universidad Nacional de Asunción

Universidade Federal da Paraíba

Universidade Federal de Campina Grande

Universidade de São Paulo

Guyra Paraguay

Universidade Federal de Pernambuco

Universidad Autónoma Agraria Antonio Narro

Universidade Federal do Rio Grande do Norte

Centre National de la Recherche Scientifique

Universidade Federal de São Paulo

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Estimulación del crecimiento de Thypa dominguensis con bacterias promotoras de crecimiento vegetal

Investigaciones y Estudios - UNA (2020)

Juliana Moura Mendes Andrea Alejandra Arrúa Alvarenga Samudio Oggero Antonio Cinthia Cazal Lourdes Martinez Rojas

Las especies de Typha sp son utilizadas en tratamientos de fitorremediación de manera exitosa. CEMIT - DGICT - UNA ha propuesto como alternativa a problemática del lago Ypacaraí, el uso de esta planta para su recuperación. El objetivo de este trabajo fue utilizar especies de Bacillus sp. para estimular la germinación de las semillas y aumentar el vigor y crecimiento de las plántulas de Thypa dominguensis. Para ello, se aislaron Bacillus sp. de semillas de Thypa dominguensis y se seleccionaron dos aislados que fueron utilizados para el tratamiento de las mismas mediante la inoculación con suspensiones bacterianas; las semillas fueron sembradas y acondicionadas en casa de vegetación por 30 días, al cabo de los cuales se procedió con las evaluaciones y se observó que las plantas tratadas con laUniversidad Nacional de Asunción. Dirección General de Investigaciones Científicas y Tecnológicas. San Lorenzo, Paraguays bacterias presentaron mejor porcentual de germinación, mayor longitud y biomasa con relación al testigo, demostrando que el tratamiento fue eficiente en la estimulación del crecimiento y aumento de la biomasa.

10.47133/ieuna2

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Prevalence of Candida tropicalis and Candida krusei in onychomycosis in João Pessoa, Paraiba, Brazil from 1999 to 2010

Anais da Academia Brasileira de Ciências (2015)

Juliana Moura Mendes LUIS A.S. RODRIGUES Fillipe de Oliveira Pereira Edeltrudes de Oliveira Lima

Over time, as the etiology of onychomycosis has developed, yeasts from the genus Candida have emerged as important etiological agents. This study aimed to determine the frequency of yeast caused onychomycosis in Joao Pessoa, Paraíba, Brazil from 1999 to 2010. A retrospective study from January 1999 to December 2010 evaluated the results of onychomycosis positive direct mycological exams (DME) - for yeast and realized in the Hemato(r) Clinical Laboratory. Women were the most affected by onychomycosis which occur preferentially in adults, and the toenails are the favorite yeast targets. The prevalent yeasts were Candida tropicalis and C. krusei.

Candida krusei

Etiology

10.1590/0001-3765201520130418

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Citations (20)

Dermatophytes develop resistance to the monoterpenes geraniol and citronellol

Revista cubana de farmacia (2020)

Gustavo Nunes Cardoso Kaltz Victor Souza Silva Maria Islaine de Oliveira Lima Juliana Moura Mendes Fillipe de Oliveira Pereira

Introduction: Dermatophytoses are fungal infections whose treatment has been the subject of much concern throughout the world. The emergence of antifungal resistance (azoles) in clinical therapeutics is well known. However, few studies demonstrate the capacity of dermatophytes to develop resistance against natural products such as terpenes. The monoterpenes geraniol and citronellol have recognized antifungal potential and are found in various essential oils. Objectives: To investigate the capacity of Trichophyton rubrum , Microsporum canis, and Microsporum gypseum to acquire resistance against citronellol and geraniol. Methods: The minimum inhibitory concentration of the tested drugs was determined by microdilution. The fungal strains were subjected to eight successive subcultures in Sabouraud dextrose agar containing the monoterpenes in sub-inhibitory concentrations. After this period of adaptation, the susceptibility profile to drugs was assessed by microdilution. Finally, after eight passages in culture medium without the drugs, resistance stability was again evaluated by microdilution test. Results: From the initial citronellol and geraniol minimum inhibitory concentration values we observed an increase. In addition, a cross over effect was observed against the azole compounds. Finally, the fungi reversed their profiles of resistance against the natural drugs and showed no reversal in resistance to azoles. Although in dermatophytes the development of resistance to monoterpenes was observed, the phenomenon was not stable, as was observed against the azole drugs. Conclusions: Citronellol and geraniol were active against resistant isolates even after dis-habituation. Thus these monoterpenes present themselves as potential therapeutic alternatives with fewer complications in the emergence of resistance.

Citronellol

Broth microdilution

Monoterpene

Griseofulvin

Terpene

Azole

Dermatophyte

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Citations (1)

First Report of a Leaf Blight Caused by Pyricularia pennisetigena on Cenchrus echinatus in Paraguay

Plant Disease (2021)

Cinthia Cazal Yessica Magaliz Reyes-Caballero Alice Rocío Chávez Pastor Pérez‐Estigarribia Andrés José Armadans Rojas.

The genus

10.1094/pdis-02-21-0287-pdn

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Citations (5)

Effects of the essential oil of Cymbopogon winterianus against Candida albicans

Revista Pan-Amazônica de Saúde (2015)

Wylly Araújo de Oliveira Juliana Moura Mendes Paulo Alves Wanderley Rita Baltazar de Lima Edeltrudes de Oliveira Lima

10.5123/s2176-62232015000300003

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Investigation on mechanism of antifungal activity of eugenol againstTrichophyton rubrum

Medical Mycology (2012)

Fillipe de Oliveira Pereira Juliana Moura Mendes Edeltrudes de Oliveira Lima

Trichophyton rubrum is a worldwide agent responsible for chronic cases of dermatophytosis which have high rates of resistance to antifungal drugs. Attention has been drawn to the antimicrobial activity of aromatic compounds because of their promising biological properties. Therefore, we investigated the antifungal activity of eugenol against 14 strains of T. rubrum which involved determining its minimum inhibitory concentration (MIC) and effects on mycelial growth (dry weight), conidial germination and morphogenesis. The effects of eugenol on the cell wall (sorbitol protect effect) and the cell membrane (release of intracellular material, complex with ergosterol, ergosterol synthesis) were investigated. Eugenol inhibited the growth of 50% of T. rubrum strains employed in this study at an MIC = 256 μg/ml, as well as mycelial growth and conidia germination. It also caused abnormalities in the morphology of the dermatophyte in that we found wide, short, twisted hyphae and decreased conidiogenesis. The results of these studies on the mechanisms of action suggested that eugenol exerts antifungal effects on the cell wall and cell membrane of T. rubrum. Eugenol act on cell membrane by a mechanism that seems to involve the inhibition of ergosterol biosynthesis. The lower ergosterol content interferes with the integrity and functionality of the cell membrane. Finally, our studies support the potential use of the eugenol as an antifungal agent against T. rubrum.

Ergosterol

10.3109/13693786.2012.742966

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Citations (101)

Trichothecene Genotype Profiling of Wheat Fusarium graminearum Species Complex in Paraguay

Toxins (2022)

Andrea Alejandra Arrúa Alvarenga Julio César Masaru Iehisa Ouchi Cinthia Cazal Juliana Moura Mendes Adans A. Colmán

Paraguay is a non-traditional wheat-producing country in one of the warmest regions in South America. Fusarium Head Blight (FHB) is a critical disease affecting this crop, caused by the Fusarium graminearum species complex (FGSC). A variety of these species produce trichothecenes, including deoxynivalenol (DON) and its acetylated forms (3-ADON and 15-ADON) or nivalenol (NIV). This study characterized the phylogenetic relationships, and chemotype diversity of 28 strains within FGSC collected from wheat fields across different country regions. Phylogenetic analysis based on the sequence of elongation factor-1α gene (EF-1α) from 28 strains revealed the presence of four species in the FGSC: F. graminearum sensu stricto, F. asiaticum, F. meridionale and F. cortaderiae. Ten strains selected for further analysis revealed that all F. graminearum strains were 15-ADON chemotype, while the two strains of F. meridionale and one strain of F. asiaticum were NIV chemotype. Thus, the 15-ADON chemotype of F. graminearum sensu stricto was predominant within the Fusarium strains isolated in the country. This work is the first report of phylogenetic relationships and chemotype diversity among Fusarium strains which will help understand the population diversity of this pathogen in Paraguay.

Chemotype

Sensu stricto

10.3390/toxins14040257

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Species Identification and Mycotoxigenic Potential of Aspergillus Section Flavi Isolated from Maize Marketed in the Metropolitan Region of Asunción, Paraguay

Microorganisms (2023)

Juliana Moura Mendes Cinthia Cazal C. Benítez de Rojas Francisco Ferreira Pastor Pérez‐Estigarribia

Zea mays var. amylacea and Zea mays var. indurata are maize ecotypes from Paraguay. Aspergillus section Flavi is the main spoilage fungus of maize under storage conditions. Due to its large intraspecific genetic variability, the accurate identification of this fungal taxonomic group is difficult. In the present study, potential mycotoxigenic strains of Aspergillus section Flavi isolated from Z. mays var. indurata and Z. mays var. amylacea that are marketed in the metropolitan region of Asunción were identified by a polyphasic approach. Based on morphological characters, 211 isolates were confirmed to belong to Aspergillus section Flavi. A subset of 92 strains was identified as Aspergillus flavus by mass spectrometry MALDI-TOF and the strains were classified by MALDI-TOF MS into chemotypes based on their aflatoxins and cyclopiazonic acid production. According to the partial sequencing of ITS and CaM genes, a representative subset of 38 A. flavus strains was confirmed. Overall, 75 A. flavus strains (86%) were characterized as producers of aflatoxins. The co-occurrence of at least two mycotoxins (AF/ZEA, FUM/ZEA, and AF/ZEA/FUM) was detected for five of the Z. mays samples (63%). Considering the high mycological bioburden and mycotoxin contamination, maize marketed in the metropolitan region of Asunción constitutes a potential risk to food safety and public health and requires control measures.

Cyclopiazonic acid

10.3390/microorganisms11081879

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Citations (2)

Occurrence of Deoxynivalenol and Ochratoxin A in Beers and Wines Commercialized in Paraguay

Toxins (2019)

Andrea Alejandra Arrúa Alvarenga Juliana Moura Mendes Pablo David Arrúa Francisco Ferreira Gabriela E Caballero

Alcoholic beverages can be contaminated with mycotoxins. Ochratoxin A (OTA) is the most frequently detected mycotoxinin wine and is produced by several species of Aspergillus. This mycotoxin is nephrotoxic and carcinogenic. In beer, the most commonly identified mycotoxin is deoxynivalenol (DON). Ingestion of food contaminated with DON has been associated with adverse gastrointestinal effects. Despite the harmful effects of mycotoxins on health, there are no regulations regarding their limits in alcoholic beverages in Paraguay. Here we determine the presence of OTA and DON in wine and beer, respectively. Four commercial brands of wine and twenty-nine brands of craft and industrial beerwere tested by the Agra quant ELISA method. One brand of wine was positive for OTA and seven brands of beer (one of them craft) were positive for DON. The values found for both toxins are below the recommended maximum intake proposed by international standards. Giving the high consumption of these products in the country, regulations and monitoring systems mustbe established to check the maximum levels of mycotoxins allowed in alcoholic beverages.

Ochratoxins

Food contaminant

10.3390/toxins11060308

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Citations (14)

Increasing antibiotic activity against a multidrug-resistantAcinetobacterspp by essential oils ofCitrus limonandCinnamomum zeylanicum

Natural Product Research (2011)

Felipe Queiroga Sarmento Guerra Juliana Moura Mendes Janiére Pereira de Sousa Maria Flaviana Bezerra Morais‐Braga Bernadete Santos

The genus Acinetobacter has gained importance in recent years due to involvement in serious infections and antimicrobial resistance. Many plants have been evaluated not only for direct antimicrobial activity, but also as resistance modifying agents. The Essential oil of Citrus limon (EOCL) addition at 156.25 µgmL(-1) (MIC/8) sub-inhibitory concentration in the growth medium led to MIC decrease for amikacin, imipenem and meropenem. The Essential oil of Cinnamomum zeylanicum (EOCZ) addition at 78.125 µg mL(-1) (MIC/8) sub-inhibitory concentrations in the growth medium caused drastic MIC reduction of amikacin. Results of combining antibiotics and essential oils had shown us a synergistic effect with both essential oils/amikacin combinations. An additive effect was observed with the combinations of both essential oils and gentamicin. The results of this study suggest that essential oil of C. limon and C. zeylanicum may suppress the growth of Acinetobacter species and could be a source of metabolites with antibacterial modifying activity.

Cinnamomum zeylanicum

Amikacin

10.1080/14786419.2011.647019

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Citations (61)