Rohit Soni

Guru Nanak Dev University

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Asiya Nazir

Abu Dhabi University

Bhupinder Singh Chadha

Guru Nanak Dev University

Harvinder Singh Saini

Guru Nanak Dev University

H. S. Saini

Guru Nanak Dev University

Rajesh Kumari Manhas

Guru Nanak Dev University

B.S. Chadha

Guru Nanak Dev University

Manju Sharma

Institute of Chemical Technology

Harinder Singh Oberoi

National Institute of Food Technology Entrepreneurship and Management

Amarjeet Kaur

Guru Nanak Dev University

Ankit Sharma

Chandigarh University

Cooperative Institutions

Guru Nanak Dev University

Punjab Agricultural University

Indian Council of Agricultural Research

The Ohio State University

University of Manitoba

Swami Keshwanand Rajasthan Agricultural University

Sri Karan Narendra Agriculture University, Jobner

Chaitanya Hospital And Nursing Home

New York University

Guru Gobind Singh Indraprastha University

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NOVEL SOURCES OF FUNGAL CELLULASES OF THERMOPHILIC / THERMOTOLERANT FOR EFFICIENT DEINKING OF COMPOSITE PAPER WASTE

DOAJ (DOAJ: Directory of Open Access Journals) (2008)

Rohit Soni B.S. Chadha H. S. Saini

Deinking

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Citations (0)

Evaluation of Glycosyl Hydrolases in the Secretome of Aspergillus fumigatus and Saccharification of Alkali-Treated Rice Straw

Applied Biochemistry and Biotechnology (2010)

Manju Sharma Rohit Soni Asiya Nazir Harinder Singh Oberoi Bhupinder Singh Chadha

Hydrolase

10.1007/s12010-010-9064-3

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Citations (50)

Novel sources of fungal cellulases for efficient deinking of composite paper waste

BioResources (2008)

Rohit Soni Asiya Nazir Bhupinder Singh Chadha H. S. Saini

Twenty thermophilic/thermotolerant fungal strains were isolated from compositing soils and screened for production of different enzymes (Endoglucanases, β-glucosidase, Fpase and xylanases) to assess their deinking efficiency. Three isolates, Aspergillus sp. AMA, Aspergillus terreus AN1,and Myceliophthora fergusii T4I, identified on the basis of morphological and sequencing of amplified ITS1-5.8S-ITS2 rDNA region, showed significant deinking of composite waste paper (70% magazine and 30% Xerox copier/ laser print paper waste) as well as improved properties (brightness, tensile strength, tear index) of recycled paper sheets. The chosen strains Aspergillus sp. AMA, Aspergillus terreus AN1and Myceliophthora fergusii T4I, showed 53, 52.7, and 40.32% deinking with increase in brightness by 4.32, 3.56, and 3.01 % ISO, respectively. These cultures were found to produce multiple endoglucanases and were characterized to lack a cellulose binding module (CBD), which may be responsible for their better deinking efficiency.

Deinking

Aspergillus terreus

Aspergillus oryzae

10.15376/biores.3.1.234-246

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Citations (33)

Purification and characterization of an endoglucanase from Aspergillus terreus highly active against barley β-glucan and xyloglucan

World Journal of Microbiology and Biotechnology (2009)

Asiya Nazir Rohit Soni Harvinder Singh Saini Rajesh Kumari Manhas Bhupinder Singh Chadha

Xyloglucan

Aspergillus terreus

Carboxymethyl cellulose

10.1007/s11274-009-0001-y

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Citations (45)

A Study for Affordable Grey Water Management: Using Microbial Fuel Cells and Ultrasonic Purification for Sustainable Water Recycling

Rohit Soni Megha Sharma Ankit Sharma Kailash Prajapat Rinku Simar

10.2139/ssrn.5042187

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Citations (0)

Regulation of expression of multiple beta-glucosidases of Aspergillus terreus and their purificaiton and characterization,

BioResources (2008)

Asiya Nazir Rohit Soni Harvinder Singh Saini Rajesh Kumari Manhas Bhupinder Singh Chadha

This study reports the regulation and purification of b-glucosidases from a thermotolerant Aspergillus terreus AN1 strain, previously reported for efficient deinking of composite paper waste. The differential expression of four beta-glucosidase isoforms, in response to carbon sources in production medium, was studied by electrophoretically resolving proteins by polyacrylamide gel electro-phoresis analysis (PAGE) and developing zymograms using methylum-belliferyl b-D glucoside as substrate. Three b-glucosidases (bGI, bGII & bGIII) were purified using chromatographic techniques. SDS-PAGE revealed the respective molecular masses of bGI, bGII, and bGIII, as 29, 43, and 98 KDa, and isoelectric point (pI) to be 2.8, 3.7, and 3.0. The b-glucosidases exhibited diverse pH and temperature optima as well as stability. b-Glucosidase I (bGI) specifically recognized pNP-b-glucopyranoside (pNPG) as a substrate, whereas, b-glucosidase II (bGII) and III (bGIII) also showed activities against cellobiose and salicin. In contrast to bGII and bGIII, the activity of bGI was positively influenced in the presence of hexoses/pentoses and alcohols. Km and Vmax for hydrolysis of pNPG by bGI, bGII, andbGIII were found to be 14.2 mM and 166.9 µmol -1mg protein -1, 4.37 mM, and 34.7 µmol -1mg proteins -1, and 11.1 mM and 378.7µ mol -1 mg protein -1, respectively.

Aspergillus terreus

Glucosidases

Salicin

Beta-glucosidase

10.15376/biores.4.1.155-171

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Citations (9)

NOVEL SOURCES OF FUNGAL CELLULASES FOR EFFICIENT DEINKING OF COMPOSITE PAPER WASTE

Rohit Soni Asiya Nazir Bhupinder Singh Chadha H. S. Saini

Twenty thermophilic/thermotolerant fungal strains were isolated from compositing soils and screened for production of different enzymes (Endoglucanases, β-glucosidase, Fpase and xylanases) to assess their deinking efficiency. Three isolates, Aspergillus sp. AMA, Aspergillus terreus AN1, and Myceliophthora fergusii T4I, identified on the basis of morphological and sequencing of amplified ITS1-5.8S-ITS2 rDNA region, showed significant deinking of composite waste paper (70% magazine and 30% Xerox copier/ laser print paper waste) as well as improved properties (brightness, tensile strength, tear index) of recycled paper sheets. The chosen strains Aspergillus sp. AMA, Aspergillus terreus AN1 and Myceliophthora fergusii T4I, showed 53, 52.7, and 40.32% deinking with increase in brightness by 4.32, 3.56, and 3.01 % ISO, respectively. These cultures were found to produce multiple endoglucanases and were characterized to lack a cellulose binding module (CBD), which may be responsible for their better deinking efficiency.

Deinking

Aspergillus terreus

Source

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Citations (26)

Molecular Profiling and Optimization of Cellulase Production by Thermotolerant Aspergillus terreus AN1

Current Biotechnology (2012)

Asiya Nazir Rohit Soni B.S. Chadha

This paper reports molecular characterization and cellulase activity profiling of thermotolerant Aspergillus strains isolated from composting soils/ degrading paper waste. A. terreus strain AN1 was found to be a good source of cellulases that showed efficient biodeinking of mixed office waste paper. The culture was optimized for production of endoglucanase, β-glucosidase, FPase and xylanase on rice straw containing solidified culture medium employing Box Behnken design of experiments to study the influence of pH, moisture content and corn steep liquor on enzyme production. A. terreus under optimized conditions produced 162, 955, 6.6, 3027 (units/g of substrate) of endoglucanase, β- glucosidase, Fpase, and xylanase corresponding to 5.7, 5.4, 2.5 and 1.1 folds higher enzyme activities when compared to those obtained under unoptimized conditions. Isoelectric focusing (IEF) profiling of the crude extract showed that A. terreus produced three endoglucanase isoforms corresponding to pI values of 3.0, 3.9 and 5.9 that were functionally distinct and showed differences in their substrate specificity. Furthermore, four isoforms of β-glucosidase and eleven multiple forms of xylanase distributed over a wide range of pI were also detected. Keywords: Molecular characterization, cellulase activity profiling, thermotolerant Aspergillus strains, biodeinking, endoglucanase, β-glucosidase, FPase, xylanase, cellulase production, Box Behnken design, Isoelectric focusing (IEF), Response Surface Methodology, PAGE, rice straw, Endogluconases.

Aspergillus terreus

10.2174/2211550111201040336

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Citations (1)

REGULATION OF EXPRESSION OF MULTIPLE BETA- GLUCOSIDASES OF ASPERGILLUS TERREUS AND THEIR PURIFICATION AND CHARACTERIZATION

DOAJ (DOAJ: Directory of Open Access Journals) (2009)

Asiya Nazir Rohit Soni Harvinder Singh Saini Rajesh Kumari Manhas Bhupinder Singh Chadha

Aspergillus terreus

Glucosidases

Beta-glucosidase

BETA (programming language)

Source