V. Pratheepa

Universidade do Porto

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N. Sukumaran

Manonmaniam Sundaranar University

Vı́tor Vasconcelos

Universidade do Porto

N. S. Hari Narayana Moorthy

Indira Gandhi National Tribal University

Marisa Silva

Centro Hospitalar Lisboa Norte

S. Ramesh

University of Ottawa

Anoop Alex

Universidade do Porto

G. Kamalanathan

Annamalai University

Agostinho Antunes

Universidade do Porto

Vasanthanathan Poongavanam

Uppsala University

D. Madasamy

Manonmaniam Sundaranar University

Cooperative Institutions

Universidade do Porto

Centro Interdisciplinar de Investigação Marinha e Ambiental

Manonmaniam Sundaranar University

Rede de Química e Tecnologia

University of Aveiro

Faculdade de Tecnologia e Ciências

Aligarh Muslim University

Research Center for Natural Resources, Environment and Society

Uppsala University

Annamalai University

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Immunomodulatory effect of certain plant leaf extracts on freshwater fish infected by bacterial

Shodhganga (2007)

V. Pratheepa

Fresh water fish

Source

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Citations (0)

Emergent Toxins in North Atlantic Temperate Waters: A Challenge for Monitoring Programs and Legislation

Toxins (2015)

Marisa Silva V. Pratheepa Luís M. Botana Vı́tor Vasconcelos

Harmful Algal Blooms (HAB) are complex to manage due to their intermittent nature and their severe impact on the economy and human health. The conditions which promote HAB have not yet been fully explained, though climate change and anthropogenic intervention are pointed as significant factors. The rise of water temperature, the opening of new sea canals and the introduction of ship ballast waters all contribute to the dispersion and establishment of toxin-producing invasive species that promote the settling of emergent toxins in the food-chain. Tetrodotoxin, ciguatoxin, palytoxin and cyclic imines are commonly reported in warm waters but have also caused poisoning incidents in temperate zones. There is evidence that monitoring for these toxins exclusively in bivalves is simplistic and underestimates the risk to public health, since new vectors have been reported for these toxins and as well for regulated toxins such as PSTs and DSTs. In order to avoid public health impacts, there is a need for adequate monitoring programs, a need for establishing appropriate legislation, and a need for optimizing effective methods of analysis. In this review, we will compile evidence concerning emergent marine toxins and provide data that may indicate the need to restructure the current monitoring programs of HAB.

Marine toxin

Ballast

10.3390/toxins7030859

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Citations (35)

Bacterial diversity and tetrodotoxin analysis in the viscera of the gastropods from Portuguese coast

Toxicon (2016)

V. Pratheepa Anoop Alex Marisa Silva Vı́tor Vasconcelos

Alphaproteobacteria

Phylotype

Stenotrophomonas

10.1016/j.toxicon.2016.06.003

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Citations (23)

Immunomodulatory activity ofAegle marmelosin freshwater fish (Catla catla) by non-specific protection

Pharmaceutical Biology (2010)

V. Pratheepa D. Madasamy N. Sukumaran

Context: The production of fish is limited by infectious diseases; when a fish grows in an immunosuppressed condition, it becomes highly susceptible to disease.Objective: The present research work investigates immunomodulatory action of Aegle marmelos (Linn.) Corr. Serr. (Rutaceae) in Catla catla Hamilton (Cyprinidae) for enhancing immune protection of the fish against bacterial infections.Material and methods: Doses of 0, 5, 10, 15, 20, 25 and 30 g aqueous plant leaf extract/kg feed were administered orally to the freshwater fish, Catla catla for a period of 30 days to investigate its efficiency to enhance the non-specific immune responses against the fish pathogen Pseudomonas aeruginosa (Schröter) Migula (Pseudomonadaceae).Results and discussion: The fish were challenged with pathogens through water medium for 30 days and the immunomodulatory effect of the Aegle marmelos was evaluated on the blood samples every 5 days untill 15 days after infection. The results obtained from the study shows that the 25 g leaf extract/kg of feed was found to be competent to enhance optimum immune response. The effectiveness of the immunostimulant action was found to be best for the first 5 days after challenging with pathogen and subsequently, the immune response was found to decline in all the concentrations of plant extract.Conclusion: The results of the study will be helpful for further investigation in the field to improve the immunocompetence of fish against bacterial pathogens.

Catla

Immunostimulant

Fresh water fish

10.3109/13880209.2010.496086

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Citations (8)

Enzymatic and Protective Effect Analysis on Pathogen Infected Cyprinus carpio using Euphorbia hirta Included Medicated Feed

Pharmacologia (2011)

V. Pratheepa N. Sukumaran

Euphorbia

10.5567/pharmacologia.2012.52.56

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Citations (6)

Effect ofEuphorbia hirtaplant leaf extract on immunostimulant response ofAeromonas hydrophilainfectedCyprinus carpio

PeerJ (2014)

V. Pratheepa N. Sukumaran

The main objective of the present study is to improve the immune power of Cyprinus carpio by using Euphorbia hirta plant leaf extract as immunostimulants. The haematological, immunological and enzymatic studies were conducted on the medicated fish infected with Aeromonas hydrophila pathogen. The results obtained from the haematological studies show that the RBC count, WBC count and haemoglobin content were increased in the infected fish at higher concentration of leaf extract. The feeds with leaf extract of Euphorbia hirta were able to stimulate the specific immune response by increasing the titre value of antibody. It was able to stimulate the antibody production only up to the 5th day, when fed with higher concentrations of (25 g and 50 g) plant leaf extract. The plant extract showed non-specific immune responses such as lysozyme activity, phagocytic ratio, NBT assay, etc. at higher concentration (50 g) and in the same concentration (50 g), the leaf extract of Euphorbia hirta significantly eliminated the pathogen in blood and kidney. It was observed that fish have survival percentage significantly at higher concentration (50 g) of Euphorbia hirta, when compared with the control. The obtained results are statistically significant at P < 0.05 and P < 0.01 levels. This research work suggests that the plant Euphorbia hirta has immunostimulant activity by stimulating both specific and non-specific immunity at higher concentrations.

Euphorbia

Immunostimulant

10.7717/peerj.671

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Citations (46)

Identification of the sources for groundwater salinization in the coastal aquifers of Southern Tamil Nadu, India

Environmental Earth Sciences (2015)

V. Pratheepa S. Ramesh N. Sukumaran A.G. Murugesan

Saltwater intrusion

Saline water

Dominance (genetics)

10.1007/s12665-015-4303-0

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Citations (13)

Immunomodulatory effect ofAegle marmelosleaf extract on freshwater fishCyprinus carpioinfected by bacterial pathogenAeromonas hydrophila

Pharmaceutical Biology (2010)

V. Pratheepa S. Ramesh N. Sukumaran

Context: Aquatic organisms (especially fish) require potent defense mechanisms to protect themselves against pathogen invasion and disease formation. The use of immunostimulants in fish culture can prevent the diseases through augmentation of both specific and non-specific immunity.Objective: A study was conducted to investigate the efficacy of different dietary doses of Aegle marmelos (Linn.) Corr. Serr. (Rutaceae) leaf extract for the immune response and the disease resistance of the freshwater fish, Cyprinus carpio Linn. (Cyprinidae) infected by Aeromonas hydrophila Chester (Aeromonadaceae).Materials and Methodology: Hematological, specific immune response, non-specific immune response and enzyme assay studies were performed on fish and were scrutinized after 50 days of feeding trial.Results: Fish were challenged with Aeromonas hydrophila at a dose of 1.5 × 104 cells/mL through intraperitoneal injection, and the hematological changes, the immune response, the enzyme activity and the disease resistance of Cyprinus carpio against the pathogen were also studied for 20 days at 5-day intervals.Discussion: The results obtained from the study demonstrated that the fish fed with leaf extract of Aegle marmelos incorporated into feed significantly enhanced the red blood cell count, white blood cell count, hemoglobin, phagocytic activity, nitroblue tetrazolium chloride assay, lysozyme, pathogen clearance and enzyme activity compared with the control group. The survivability was higher in the fish which consumed leaf extract-incorporated feed, and the fish group fed with 5 g diet showed highest percentage survival of the fish.Conclusion: These results indicate that Aegle marmelos stimulates the immunity and makes the freshwater fish Cyprinus carpio more resistant to Aeromonas hydrophila.

Common carp

10.3109/13880201003713598

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Citations (44)

Effect of Ultrasonication on Microbial, Chemical and Sensory properties of Juices and its Kinetic aspects: A Review

International Journal of ChemTech Research (2020)

V. Pratheepa G. Kamalanathan

Ultrasound is a novel and innovative technology which is rapidly emerging in food industry. There was an increased consumer demand on new methods of food processing that have a reduced impact on nutritional content and overall food quality. Ultrasonic processing is still infancy and requires a great deal of future research work in order to develop industrial equipment. Ultrasound is found to be an effective method for microbial inactivation and greater efficiency is obtained by combination of ultrasound with heat and pressure. This technique is also used as an analytical technique to provide information about the physiochemical properties of foods. Ultrasound is more beneficial because less processing time, better product quality, less hazards and being eco-friendly. This review summarizes mechanism, application and effects on various parameters of ultrasound in fruit juices.

Sonication

Food Products

10.20902/ijctr.2019.130111

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Citations (2)

Kinetics and Effects of Ultrasonication on Physiochemical, Microbial and Sensory Properties of Grape Juice during storage periods

International Journal of ChemTech Research (2019)

V. Pratheepa G. Kamalanathan R. M. Meyyappan

The present work is aimed to study the kinetics and effects of ultrasonication on physiochemical, microbial and sensory properties of grape juice during storage periods of 90 days.The main objective of the present work is to increase the shelf life of the grape juice but without adding any chemical preservative.The grape juice was ultrasonicated at room temperature at various time intervals of 5,10,15,20 and 30 minutes to determine the pH, TSS, Titratable acidity, Ascorbic acid, Reducing sugar, Microbial load, and Sensory Properties during storage periods.It was observed that the optimum treatment for grape juice was 20 min ultrasonicated sample for 15 days and it had pH of 3.9, TSS of 17.6ºBrix, Titratable acidity of 0.84 (g/100ml), reducing sugar of 10.3 (µg/ml), ascorbic acid of 2.65 (mg/100g) microbial load of 10 (CFU/ml), and sensory score of 9.According to FDA, 5 log reductions in microbial load were attained 20 and 30 min ultrasonicated juice samples.The shelf life of grape juice was increased upto 15 days.The results suggest that ultrasound treatment technology could be potentially employed for the processing of grape juice and could improve its quality and safety.

Sonication

10.20902/ijctr.2019.120519

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Citations (2)