Stromal cell derived factor - 1 (SDF-1) is expressed by bone marrow derived stromal cells and stromal tissues in multiple organs. SDF-1 possesses diverse physiological and biochemical functions concerning autoimmunity and inflammation. SDF-1 gene polymorphisms play various roles in immune related diseases. This study aimed to evaluate association of SDF-1β G801A gene polymorphism and type 2 diabetes mellitus (T2DM) as regard prevalence and glycemic control. The study included hundred Egyptian subjects; 50 patients with T2DM recruited from the Internal Medicine Department, Beni-Suef University Hospital, and 50 age and sex matched healthy subjects. Participants were subjected to full clinical examination and routine laboratory investigations including fasting blood glucose, 2 hours post prandial blood glucose, serum creatinine, lipid profile and glycated hemoglobin (HbA1c). SDF-1β G801A gene polymorphism was analyzed using polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP). The frequency of GG and AG genotypes was higher in patients compared to controls with no statistical significance. Higher frequency of homozygous GG genotype and G allele were observed among patients with poor glycemic control (HbA1c ≥ 8% ) compared to those with good glycemic control (HbA1c < 8%) (P = 0.001 and 0.018 respectively). In conclusion, this study suggested an association between SDF-1 β gene polymorphism and type 2 diabetes mellitus in Egyptian patients. Further studies are needed to confirm our observations.

Glycated hemoglobin

Gene polymorphism

10.55133/eji.280310

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Evaluation of NPHS2 gene polymorphism (R229Q) in chronic kidney disease patients

Egyptian Journal of Medical Research (2021)

Ahmed Mohamed rashid Rania E. Sheir Thoraya Mohamed Ahmed Hanan Farhan

The aim of this study was to evaluate the role of nephrotic syndrome 2 (NPHS2) gene polymorphism (R229Q) in chronic kidney disease which has unclear etiology.This study was conducted on 80 CKD patients compared with 40 age and sex matched normal volunteers acting as a control group.All of them underwent renal function tests and were assessed for the presence of NPHS2 gene polymorphism.We noticed that (R229Q) is a common variant in Egyptian population.Its concentration was (62.5%) in CKD patients and (55%) in control group.There was no significant difference with p-value > 0.05 between patients and control groups as regards to NPHS2 genotypes and alleles.

Gene polymorphism

Etiology

10.21608/ejmr.2021.174556

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The Relation between Microvascular Complications of Type 2 Diabetic Patients and Plasma Copeptin Levels

Egyptian Journal of Medical Research (2024)

Hazem Samy Matar Noha Ali Rania E. Sheir Neveen Youssef Thoraya Mohamed Ahmed

Background: Diabetes mellitus (DM), commonest metabolic illness, is one of the major public health concerns worldwide. Objectives: To inspect the clinical importance of serum copeptin in patients of type 2 diabetes with and without nephropathy and to assess the relation between microvascular complication of type 2 diabetic patients and copeptin plasma levels. Patients and Methods: The study was conducted between November 2021 till May 2022, included 90 participants; 30 healthy age and sex matched control participants, 20 cases of DN with normoalbuminuria, 20 cases of DN with microalbuminuria, and 20 cases of DN with macroalbuminuria. Serum copeptin levels were measured by ELISA, Blood Urea Nitrogen (BUN), creatinine, Glycosylated Hemoglobin (HbA1c), and spot urinary albumin creatinine ratio (UACR) were done using spectrophotometry. Results: it was shown that serum copeptin level in patients with diabetic nephropathy with macro albuminuria was significantly lower than in healthy control people. There was no significant difference in level of serum copeptin in normal or abnormal fundus examination, neurological examination. Conclusion: serum copeptin level in patients with diabetic nephropathy with macroalbuminuria is significantly lower than in normal healthy control. There was no significant association was found between serum copeptin level, diabetic retinopathy nor neuropathy.

Copeptin

10.21608/ejmr.2023.223225.1422

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Overexpression of the giant FAT1 cadherin gene and its prognostic significance in de novo acute leukaemia patients

Comparative Clinical Pathology (2017)

Rania M. Gawdat Shereen Mohamed Elhoseiny Amira Darwish Rania E. Sheir Waleed M. El-Nabwey

10.1007/s00580-017-2409-3

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Assessment of SH3YL1 protein as a marker for diabetic nephropathy in type 2 diabetes mellitus

Egyptian Journal of Medical Research (2022)

Shimaa Gomaa Rania E. Sheir Hanan Farhan Thoraya Mohamed Ahmed Ahmed Abdelsattar

The goal of this study was to explore the association between Src homology 3 domain of SH3 domain containing Ysc84-like 1 (SH3YL1) protein and diabetic nephropathy (DN) in type 2 diabetes mellitus (T2DM).The study included 90 participants, 60 patients with T2DM all recruited from Outpatients Clinic and Internal Medicine Inpatient Department, Beni-Suef University Hospital; and 30 apparently healthy controls.Patients were sub-divided into group I: 20 patients with no albuminuria, group II: 20 patients with microalbuminuria and group III: 20 patients with macroalbuminuria.All participants were subjected to full history taking, routine laboratory investigations and serum SH3YL1 analysis using enzyme-linked immunosorbent (ELISA) assay.Serum SH3YL1 was significantly higher in T2DM patients (5.69 ± 1.6 ng/ml) versus controls (3.76 ±0.80 ng/ml) (p<0.001).There was statistical significant difference with p <0.05 between cases of group I, and each of group II, and group III as regards SH3YL1 level with lowest mean among group I.There was statistical significant positive correlation between SH3YL1 level and each of fasting blood glucose, 2hours postprandial, glycated hemoglobin, urea, creatinine, albuminuria, total cholesterol and triglycerides.SH3YL1 showed sensitivity of (88.3%) and specificity of (53.3%) at cut off value (3.85 ng/mL) among T2DM patients versus controls (p<0.001).Moreover, sensitivity of (70%, 90%, and 90%) and specificity of (56.7%, 70%, and 86.7%) at cutoff (4 ng/mL, 4.45 ng/mL, and 4.65 ng/mL) was found among groups I, II and III versus control group (p= 0.01, <0.001 and <0.001), respectively.In conclusion, SH3YL1 serum level revealed statistically significant increase among T2DM patients and showed statistical difference between the studied no albuminuria, microalbuminuria and macroalbuminuria groups.Our study suggests SH3YL1 as a promising diagnostic and prognostic marker among DN patients.

10.21608/ejmr.2022.259455

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Methylene tetrahydrofolate reductase (MTHFR) gene polymorphisms in chronic myeloid leukemia: an Egyptian study

Medical Oncology (2013)

Mervat M. Khorshied Iman Shaheen Reham E. Abu Khalil Rania E. Sheir

Genetic predisposition

10.1007/s12032-013-0794-2

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Serum osteocalcin concentration as a biomarker of osteoporosis in Egyptian patients with chronic renal failure on regular hemodialysis

Egyptian Journal of Medical Research (2024)

Ahmed S Abdelsattar Rania E. Sheir Raghda E Ibrahim E. Eid

Chronic renal failure

10.21608/ejmr.2024.305448.1644

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Assessment of public awareness about body measurements among Fayoum population

Health (2014)

Naglaa A. El-Sherbiny Mohamed Mashahit Rania E. Sheir

The prevalence of obesity is increasing world-wide. There was general agreement that BMI surveillance was an epidemiologic tool for the assessment of obesity in different populations. The aim of this study is to screen community awareness about body measurement, and to examine actual body weight with self-perception of body image. Methods: This study was a community-based survey conducted in Fayoum Governorate. We used multistage stratified random sampling to select the study household with a number of participants (582). We developed a self-administrated structured questionnaire. Anthropometric assessment weight, height and waist circumference were measured, and BMI was calculated. Results: Our results showed that the prevalence of obesity was 88.7% more in female than male. More than half of the participants knew their weight and height. Overall, 40.6% of women and 38.8% of men misclassified their own weight status by BMI. There was a statistical significant difference between knowing weight and height, and their accurate results (P = 0.000). Conclusion: Our results showed that the majority of the Fayoum population was obese. Implementation of health promotion and health education in the community should use effective nutrition education in the mass-media to raise awareness of appropriate body weight and healthy lifestyle.

Stratified Sampling

Circumference

Community Health

10.4236/health.2014.63031

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Albumin-corrected serum calcium and serum parathyroid hormone-related polypeptide determined by enzyme-linked immunosorbent assay in cirrhotic patients with hepatocellular carcinoma – a pilot study

The Egyptian Journal of Internal Medicine (2019)

Ragai Fouda Rania E. Sheir Nora Mahmoud Selim Ahmed Hammad

Abstract Context Parathyroid hormone-related peptide (PTHrP) is produced by many malignant tumors. It is responsible for most cases of hypercalcemia in patients with malignancy. Few published studies shed light on the relation between serum calcium levels and serum PTHrP levels in cirrhotic patients with hepatocellular carcinoma (HCC). Aim The aim of the current work was to evaluate serum PTHrP in cirrhotic patients with HCC and a possible correlation between serum PTHrP levels and albumin-corrected serum calcium levels in these patients. Patients and methods This is a cross-sectional study. The study included 35 cirrhotic patients with HCC (diagnosed depending upon α-fetoprotein and abdominal imaging studies). Data about their serum albumin and albumin-corrected serum calcium levels were collected. Sera of the studied patients were collected for determination of PTHrP levels by enzyme-linked immunosorbent assay (ELISA). Numerical data were summarized in the form of mean±SD. Strength of association between variables was tested using Pearson correlation coefficient. Results Approximately 8.6% of studied patients were hypercalcemic, and no statistically significant positive correlation was detected between serum PTHrP determined by ELISA and albumin-corrected serum calcium in these patients. Conclusion Approximately 8.6% of studied cirrhotic patients with HCC were hypercalcemic. NO statistically significant positive correlation was detected between serum PTHrP determined by ELISA and albumin-corrected serum calcium in these patients. Studies involving a larger number of patients could clarify the exact role of PTHrP in the development of hypercalcemia in cirrhotic patients with HCC.

Serum Albumin

10.4103/ejim.ejim_121_19

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