Dan Ji

Xiangya Hospital Central South University

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Rong Rong

Xi’an Jiaotong-Liverpool University

Haibo Li

Guangzhou Medical University

Neville N. Osborne

Royal Devon & Exeter NHS Foundation Trust

Mengling You

Xiangya Hospital Central South University

Xiaobo Xia

Central South University

Xiaobo Xia

Xiangya Hospital Central South University

Kun Xiong

Central South University

Jufang Huang

Central South University

Amin Malik Shah Abdul Majid

Australian National University

Zhou Zeng

Second Xiangya Hospital of Central South University

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Central South University

Chinese Academy of Sciences

European Foundation for the Study of Chronic Liver Failure

Xiangya Hospital Central South University

University of Chinese Academy of Sciences

Sichuan University

Sun Yat-sen University

Shanghai Jiao Tong University

University of Oxford

Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas

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Long Non-Coding RNA-MALAT1 Mediates Retinal Ganglion Cell Apoptosis Through the PI3K/Akt Signaling Pathway in Rats with Glaucoma

Cellular Physiology and Biochemistry (2017)

Haibo Li Qi Sheng You Lixin Xu Lixin Sun Aman Shah Abdul Majid

The aim of the present study is to investigate the effect of long non-coding RNA-MALAT1 (LncRNA-MALAT1) on retinal ganglion cell (RGC) apoptosis mediated by the PI3K/Akt signaling pathway in rats with glaucoma.RGCs were isolated and cultured, and monoclonal antibodies (anti-rat Thy-1, Brn3a and RBPMS) were examined by immunocytochemistry. An overexpression vector MALAT1-RNA activation (RNAa), gene knockout vector MALAT1-RNA interference (RNAi), and control vector MALAT1-negative control (NC) were constructed. A chronic high intraocular pressure (IOP) rat model of glaucoma was established by episcleral vein cauterization. The RGCs were divided into the RGC control, RGC pressure, RGC pressure + MALAT1-NC, RGC pressure + MALAT1-RNAi and RGC pressure + MALAT1-RNAa groups. Sixty Sprague-Dawley (SD) rats were randomly divided into the normal, high IOP, high IOP + MALAT1-NC, high IOP + MALAT1-RNAa and high IOP + MALAT1-RNAi groups. qRT-PCR and western blotting were used to detect the expression levels of LncRNA-MALAT1 and PI3K/Akt. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) and flow cytometry were used to detect RGC apoptosis.Immunocytochemistry revealed that the cultured RGCs reached 90% purity. Compared with the RGC pressure + MALAT1-NC group, the RGC pressure + MALAT1-RNAa group exhibited elevated expression levels of MALAT1, lower total protein levels of PI3K and Akt and decreased RGC apoptosis, while these expression levels were reversed in the RGC pressure + MALAT1-RNAi group. RGC numbers and PI3K/Akt expression levels in the high IOP model groups were lower than those in the normal group. In the high IOP + MALAT1-RNAa group, the mRNA and protein expression levels of PI3K/Akt were reduced but higher than those in the other three high IOP model groups. Additionally, RGC numbers in the high IOP + MALAT1-RNAa group were lower than those in the normal group but higher than those in the other three high IOP model groups.Our study provides evidence that LncRNA-MALAT1 could inhibit RGC apoptosis in glaucoma through activation of the PI3K/Akt signaling pathway.

MALAT1

10.1159/000484231

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Synthesis and Biological Activities of Novel 5-Methyl-1,2,4-triazoles-3-thione

Chinese Journal of Organic Chemistry (2013)

Dan Ji Junrui Lu Chunwei Xin Chunyong Peng Jiangbei Mu

新型 5-甲基-1,2,4-三唑-3-硫酮类化合物的合成及抗菌活性研究

10.6023/cjoc201211043

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Multiple-model based simulation of urban atmospheric methane concentration and the attributions to its seasonal variations: a case study in Hangzhou megacity, China

Environmental Pollution (2024)

Junqing Zhang Dan Ji Cheng Hu Timothy J. Griffis Qitao Xiao

Megacity

Atmospheric methane

Methane Emissions

10.1016/j.envpol.2024.124781

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The relationship between p53 gene cDNA mutation and p53 protein expression in colorectal adenocarcinomas

Ji Chenyang Dan Ji Duncan R. Smith Goh H S

Objective To study the relationship between p53 gene mutation and p53 protein expression in colorectal carcinomas. Metltods p53 gene mutation was detected by RT-PCR-SSCP and p53 protein was deteded by monoclonal antibody PAb1801 in the same tissue. Results In 100 patients with colorectal carcinomas, 51 (51 % ) were PO8itive for p53 gene mutation,and 62 (62% ) were PAb1801 positive shown by immunohistochemistry staining (LSAB method ). In 49 patients without p53 gene mutaion, 24 were negative and 25 positive for PAb1801. Among the 51 patients with p53 gene mUtation, 14 were negative and 37 positive for PAb1801 (Fisher's exact test,P=0. 02). Conclusion The mutation of p53 gene in colorectal carcinomas is one of the main factors which participates and affects p53 gene expression.

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Evidence That Light Can Affect Ganglion Cell Parameters in situ

Investigative Ophthalmology & Visual Science (2008)

Dan Ji N. Osborne

Affect

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Rotenone-Induced Death of RGC-5 Cells is Caspase Independent, Involves the JNK and p38 Pathways and is Attenuated by Specific Green Tea Flavonoids

Neurochemical Research (2012)

Tengku Ain Kamalden Dan Ji Neville N. Osborne

Rotenone

10.1007/s11064-012-0713-5

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Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro

Frontiers in Endocrinology (2023)

Mengling You Rong Rong Zhou Zeng Cong Fan Haibo Li

Introduction In recent years, the biological functions and important roles of long non-coding RNAs (lncRNAs) have been widely reported in many diseases. Although glaucoma is the leading cause of blindness worldwide, the specific mechanisms of lncRNAs in the pathogenesis and progression of glaucoma remain unclear. Our research aims to elucidate the differentially expressed lncRNAs and mRNAs in glaucoma and to provide a basis for further exploration of the specific mechanism of action of lncRNAs in the progression of glaucoma. Methods We performed RNA sequencing on samples from a pressurized model of R28 cells and performed bioinformatics analyses on the sequencing results. The expression consistency of lncRNAs in clinical samples from patients with glaucoma or cataracts was detected using real-time quantitative polymerase chain reaction (RT-qPCR). Results RNA sequencing results showed that lncRNAs in cluster 5 were upregulated with increasing stress after typing all significantly altered lncRNAs using k-means in a cellular stress model. KEGG analysis indicated that they were associated with neurodegenerative diseases. Differentially expressed lncRNAs were verified by RT-qPCR, and the lncRNA expression levels of AC120246.2 and XLOC_006247 were significantly higher in the aqueous humor (AH) of patients with glaucoma than in those with cataracts. For LOC102551819, there was almost no expression in the AH and trabecular meshwork in patients with glaucoma but high expression was observed in the iris. Conclusion Our research proposes potential diagnostic or intervention targets for clinical applications as well as a theoretical basis for more in-depth research on the function of lncRNAs in glaucoma.

10.3389/fendo.2023.1087442

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[Tall cell variant of papillary breast carcinoma: report of a case].

PubMed (2019)

Liming Ding Hongxiang Hu Yuejun Wang Dan Ji Liangyan Ni

报道1例乳腺高细胞亚型乳头状癌，患者为70岁女性，左乳肿块。组织学显示癌组织呈实性乳头状结构，被致密的纤维结缔组织分隔为巢状排列，癌细胞形态温和，呈立方状和高柱状排列，胞质嗜酸性或透明状，核呈圆形或卵圆形，染色质淡染，核膜清晰，核沟易见，并可见一些小核仁，轻度异型，未见明显的核分裂象和核内假包涵体。免疫表型示癌细胞特征性表达细胞角蛋白（CK）5/6、Calretinin；而甲状腺相关标志物甲状腺转录因子1（TTF1）、甲状腺球蛋白（TG），神经内分泌标志物嗜铬粒素A、突触素及肌上皮标志物p63、Calponin、S-100蛋白均呈阴性，Ki-67阳性指数<2%。雌激素受体、孕激素受体及HER2阴性，分子分型提示为基底细胞样型。分子遗传学检测结果提示第15号染色体IDH2（p.R172）点突变，同时PIK3CA及PDCD1伴随突变。结果提示乳腺高细胞亚型乳头状癌是一种罕见的原发于乳腺的低度恶性肿瘤，诊断时结合特征性的形态学、免疫表型及分子遗传学特征，并与组织形态学相似的转移性甲状腺癌及其他乳腺乳头状病变相鉴别。.

Breast carcinoma

10.3760/cma.j.issn.0529-5807.2019.10.015

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RTP801 immunoreactivity in retinal ganglion cells and its down-regulation in cultured cells protect them from light and cobalt chloride

Brain Research Bulletin (2013)

Susana del Olmo‐Aguado Claudia Núñez‐Álvarez Dan Ji Alberto García Manso Neville N. Osborne

RTP801, a stress-related protein, is activated by adverse environmental conditions and inhibits the activity of mammalian target of rapamycin (mTOR) in promoting oxidative stress-dependent cell death. RTP801 exists both in the mammalian retina and the lens of the eye. Here, we observed RTP801 immunoreactivity in some retinal ganglion cells. Intravitreal injection of cobalt chloride (CoCl2) to mimick hypoxia influenced retinal GFAP (glial fibrillary acidic protein) and heme oxygenase-1 (HO-1) levels, but did not affect RTP801 immunoreactivity or mRNA content relative to GAPDH. However, RTP801 mRNA was elevated when compared with Brn3a mRNA, suggesting that RTP801 is activated in stressed Brn3a retinal ganglion cells. In cultures of RGC-5 cells, RTP801 immunoreactivity was located in the cytoplasm and partly present in the mitochondria. An insult of blue light or CoCl2 increased RTP801 expression, which was accompanied by cell death. However, in cultures where RTP801 mRNA was down-regulated, the negative influence of blue light and CoCl2 was blunted. Rapamycin nullified the CoCl2-induced up-regulation of RTP801 and attenuated cell death. Moreover, rapamycin was non-toxic to RGC-5 cells, even at a high concentration (10 μM). The protective effect of rapamycin on RGC-5 cells caused by the inhibition of RTP801 suggests that rapamycin might attenuate retinal ganglion cell death in situ, as in glaucoma.

10.1016/j.brainresbull.2013.08.002

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Single‐cell RNA sequencing: A new opportunity for retinal research

Wiley Interdisciplinary Reviews - RNA (2021)

Mengling You Rong Rong Zhou Zeng Haibo Li Xiaobo Xia

Abstract Single‐cell RNA sequencing (scRNA‐seq) is a technology for single‐cell transcriptome analysis that can be used to characterize complex dynamics of various retinal cell types. It provides deep scrutiny into the gene expression character of diverse cell types, lending insight into all the biological processes being carried out. The scRNA‐seq is an alternative to regular RNA‐seq, which does not achieve cellular heterogeneity. The retina, is a part of the central nervous system (CNS) and consists of six types of neurons and several types of glial cells. Studying retinal cell heterogeneity is important for understanding retinal diseases. Currently, scRNA‐seq is employed to assess retina development and retinal disease pathogenesis and has improved our understanding of the relationship between the retina, its visual pathways, and the brain. Moreover, this technology provides new ideas on the sensitivity and molecular mechanisms of cell subtypes involved in retinal‐related diseases. The application of scRNA‐seq technology has given us a deeper understanding of the latest advancements and challenges in retinal development and diseases. We advocate scRNA‐seq as one of the important tools for developing novel therapies for retinal diseases. This article is categorized under: RNA Methods > RNA Analyses in Cells RNA in Disease and Development > RNA in Development RNA in Disease and Development > RNA in Disease

Cell type

10.1002/wrna.1652

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