Joana Neves

University of Aveiro

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Diogo N. Cardoso

University of Aveiro

Susana Loureiro

University of Aveiro

Catarina Malheiro

University of Aveiro

Amadeu M.V.M. Soares

University of Aveiro

Solange Costa

National Institute of Health Dr. Ricardo Jorge

Alexandre Campos

Universidade do Porto

Joana Azevedo

Universidade do Porto

Sofia Santos Costa

Universidade Nova de Lisboa

Patrícia Coelho

University of Edinburgh

Mariana Andrade

Hospital de São João

Cooperative Institutions

University of Aveiro

Universidade do Porto

Universidade do Sul de Santa Catarina

Universidade Nova de Lisboa

National Institute of Health Dr. Ricardo Jorge

University of Lisbon

Instituto de Medicina Tropical

Centro de Estudos Ambientais e Marinhos

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto

Centro Interdisciplinar de Investigação Marinha e Ambiental

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Recent genetic damage in a pesticide-exposed population: Comet assay and micronuclei in reticulocytes

Toxicology Letters (2010)

Carla Costa S. Silva Patrícia Coelho Solange Costa Joana Neves

Comet Assay

10.1016/j.toxlet.2010.03.1019

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Citations (0)

Exploring the virulence potential of Staphylococcus aureus CC121 and CC152 lineages related to paediatric community-acquired bacteraemia in Manhiça, Mozambique

Scientific Reports (2024)

Marcelino Garrine Mariana Andrade Joana Neves Inácio Mandomando Isabel Couto

Abstract Staphylococcus aureus is a frequent agent of bacteraemia. This bacterium has a variety of virulence traits that allow the establishment and maintenance of infection. This study explored the virulence profile of S. aureus strains causing paediatric bacteraemia (SAB) in Manhiça district, Mozambique. We analysed 336 S. aureus strains isolated from blood cultures of children younger than 5 years admitted to the Manhiça District Hospital between 2001 and 2019, previously characterized for antibiotic susceptibility and clonality. The strains virulence potential was evaluated by PCR detection of the Panton-Valentine leucocidin (PVL) encoding genes, lukS-PV/lukF-PV , assessment of the capacity for biofilm formation and pathogenicity assays in Galleria mellonella . The overall carriage of PVL-encoding genes was over 40%, although reaching ~ 70 to 100% in the last years (2014 to 2019), potentially linked to the emergence of CC152 lineage. Strong biofilm production was a frequent trait of CC152 strains. Representative CC152 and CC121 strains showed higher virulence potential in the G. mellonella model when compared to reference strains, with variations within and between CCs. Our results highlight the importance of monitoring the emergent CC152-MSSA-PVL + and other lineages, as they display important virulence traits that may negatively impact the management of SAB paediatric patients in Manhiça district, Mozambique.

Galleria mellonella

Carriage

10.1038/s41598-024-61345-3

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Citations (1)

Estudos em educação

Sérgio A. Gomes Juliana A.S.A. Oliveira Marisângela Nandi Veronez Karmensita Almeida da Rocha Cardoso Maria Sirlene Pereira Schlickmann

ESTUDOS EM EDUCAÇÃO: perspectivas teóricas e metodológicas é o primeiro livro de um projeto coletivo do Programa de Pós-Graduação em Educação (PPGE) da Universidade do Sul de Santa Catarina, sustentado no tripé ensino, pesquisa e extensão. A finalidade do projeto coletivo do PPGE-Unisul consiste na produção de subsídios que orientem e respaldem novos estudos em educação. Diante de tal finalidade, é indispensável à divulgação da produção científica já existente, a fim de se avançar no processo de superação das fragilidades metodológicas, na garantia do rigor do método científico e confiabilidade na obtenção de novos resultados. Eleger a ciência como prática social inesgotável, que não se basta em si mesma, significa um constante espaço-tempo de perguntas e respostas, pressupostos postos à prova, de hipóteses comprováveis ou falsificáveis, de teorias e métodos que apenas levam ao conhecimento quando são capazes de identificar os enigmas do desconhecido. É nessa arena que se geram os processos de conhecimento. É nessa trama de inquietudes que se tecem as experiências teóricas e metodológicas que integram este livro, que está organizado em três partes intituladas: Educação, História e Política; Relações Culturais e Históricas na Educação; e Educação em Ciências. Educação, História e Política é composta por pesquisas sobre os fundamentos históricos, políticos e pedagógicos da educação; políticas e gestão da educação; história das instituições e das práticas educacionais; formação inicial e continuada de professores; e trabalho docente. Os textos que compõem a segunda parte, ?Relações Culturais e Históricas na Educação, incidem nos processos educativos e suas interfaces com a cultura, história, filosofia, sociologia, antropologia e psicologia; experiência e formação de professores; estudos sobre infância e sua educação em contextos formais e informais; relações de gênero, classe e raça/etnia na educação. As investigações apresentadas na última parte, ?Educação em Ciências, são sustentadas nos fundamentos históricos, filosóficos, epistemológicos, axiológicos, psicológicos e didático-pedagógicos da Educação em Ciências, relacionados aos processos de ensino e aprendizagem na educação básica, superior (formação inicial e continuada de professores) e em contextos não formais e informais. O livro é composto por 21 capítulos assinados por pesquisadores dos Grupos de Pesquisa Educação, Infância e Gênero (GEDIG); Estudos sobre Gestão e Práticas Educacionais (EGePE); Estudos e Pesquisas em Políticas Educacionais, História, Alfabetização e Letramento na Educação Básica (GEPPHALE); Núcleo de Pesquisa em Educação e Conservação da Biodiversidade (NUPEC-Bio); Pesquisa Interdisciplinar em Tecnologias da Informação e da Comunicação (INTERTIC); Política e Gestão da Educação; e, Teoria do Ensino Desenvolvimental na Educação Matemática (TedMat), que aceitaram o convite para divulgar os principais resultados de seus estudos. Trata-se de um evidente testemunho de pujança do projeto coletivo do PPGE, desenvolvido pelos integrantes de seus diferentes grupos de pesquisa. Nosso desejo é que esta obra ressoe nos movimentos de resistência tanto no âmbito acadêmico quanto político, realizados por pesquisadores, professores/as, estudantes da graduação e pós-graduação. Convidamos para a leitura da síntese do nosso esforço coletivo em um processo contínuo de busca por perguntas e respostas que sejam capazes de produzir e socializar conhecimentos à altura de seu tempo.

10.29327/5450559

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Copper toxicity to Folsomia candida in different soils: a comparison between nano and conventional formulations

Environmental Chemistry (2019)

Joana Neves Diogo N. Cardoso Catarina Malheiro Mélanie Kah Amadeu M.V.M. Soares

Environmental contextNanotechnology has great potential for managing agricultural plant disease. This study compares effects of various nano and conventional copper formulations on Collembola Folsomia candida, a model arthropod often used to test soil pollutants. Although toxicity varied between formulations, with a nanopesticide showing one of the highest toxicities, the differences were mainly due to the active copper compound rather than its nano formulation. AbstractNanotechnology has recently become a major asset in agricultural practices owing to the improvement and provision of solutions for plant disease management, especially in the form of nanopesticides. These products are considered as one of the main direct sources of nanomaterials in terrestrial ecosystems, and for that reason, it is essential to understand and evaluate their behaviour and toxicity in the environment. In addition, the panoply of similar compounds makes it difficult to accurately evaluate if different formulations may induce different effects on non-target organisms. We aim to compare the toxicity and bioavailability of different copper formulations (nano and non-nano) applied to the soil using the model organism Folsomia candida. Reproduction tests were performed in two different soils (Lufa 2.1 and 2.2) with two equilibrium times after spiking (0 and 48h). Depending on the copper form present in the formulation, the half maximal effective concentration (EC50) values of the non-nano pesticides varied from 274mgCukg−1 in Champion® to 3030mgCukg−1 in Cupravit® in Lufa 2.2 and 48h equilibrium. The nano formulation displayed one of the highest toxicities to collembolans, with an EC50 of 156mgCukg−1. Regarding the soil type, a higher toxicity in Lufa 2.1 was observed probably related to the soil characteristics, namely lower pH and lower organic content, which increase the metal solubility and consequently the bioavailability. The only exception was the nanopesticide Kocide 3000®, whose toxicity was higher in Lufa 2.2. This study emphasises the importance of understanding the links between metal bioavailability and toxicity to support the more robust environmental risk assessment of different Cu fungicide formulations.

EC50

Springtail

Ecotoxicity

10.1071/en19061

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Citations (26)

Biochar in soil mitigates dimethoate hazard to soil pore water exposed biota

Journal of Hazardous Materials (2020)

Catarina Malheiro Diogo N. Cardoso Joana Neves Diana L.D. Lima Valdemar I. Esteves

Amendment

10.1016/j.jhazmat.2020.123304

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Citations (13)

Responses of soil microbiome to copper-based materials (nano and bulk) for agricultural applications: An indoor-mesocosm experiment

NanoImpact (2024)

Sara Peixoto Rui G. Morgado Marija Prodana Diogo N. Cardoso Catarina Malheiro

Mesocosm

10.1016/j.impact.2024.100506

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Micronucleus Frequencies in Lymphocytes and Reticulocytes in a Pesticide-Exposed Population in Portugal

Journal of Toxicology and Environmental Health (2011)

Carla Costa Susana Silva Joana Neves Patrícia Coelho Solange Costa

A wide range of chemical products known to be acutely toxic is currently used in the agricultural sector, including numerous pesticides with different compositions. Nevertheless, the effects in human health as result of chronic exposure to low levels are not yet completely understood. The methodology for determination of micronuclei (MN) in lymphocytes (CBMN) is well established, and accumulating data demonstrated a correlation to enhanced risk of cancer development. However, analysis of MN in reticulocytes (MN-RET) in humans is a recent tool on human biomonitoring. The aim of this study was to examine the influence of pesticide exposure on MN-RET and CBMN frequencies. In total, 177 individuals were studied (93 controls and 84 exposed). All individuals included in the exposed group were exposed regularly to various chemicals. Both MN-RET and CBMN were significantly higher in the exposed subjects compared to controls. The CBMN frequencies were quantitatively higher in females than males, especially within the exposed group. Smoking habits exerted no marked influence on the frequency of the biomarkers studied. A significant and positive correlation was found between both indicators. Within the exposed group, data showed that there was a significant correlation between MN-RET and recent exposure (exposure in the previous 10 d) that is not found when considering CBMN. It is conceivable that due to the short life span of reticulocytes, MN-RET were found to be more reliable to characterize recent genetic damage as opposed to CBMN.

10.1080/15287394.2011.582024

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Evaluation of Amlodipine and Imipramine Efficacy to Treat Galleria mellonella Infection by Biofilm-Producing and Antimicrobial-Resistant Staphylococcus aureus

Antibiotics (2025)

Mariana Andrade Joana Neves Maria Gabriela Grego Rodrigues Bento Joana Marques Sofia G. Seabra

Background/Objectives: Antimicrobial-resistant Staphylococcus aureus is a growing threat to human health for which alternative therapeutic options are needed. In this study, we aimed to evaluate the efficacy of amlodipine (AML) and imipramine (IMI) to treat S. aureus infection in the Galleria mellonella larval model by targeting efflux and biofilms, which are relevant contributors to antimicrobial resistance and virulence in S. aureus. Methods: In-house reared G. mellonella were used in virulence assays to determine the infective dose of two S. aureus strains differing in the expression of norA (gene encoding the native NorA efflux pump). Toxicology assays were conducted to determine the drugs' LD50 for G. mellonella. Drug efficacy assays were performed to evaluate the potential of amlodipine, imipramine and the control drugs ciprofloxacin (CIP) and enalapril (ENA) to clear S. aureus infection in G. mellonella. Results: Survival analysis defined the infective dose as 1 × 107 CFU/larva for both strains. High LD50 values were determined (CIP: >1000 mg/kg; AML: >640 mg/kg; IMI: 1141 mg/kg; ENA: >1280 mg/kg), revealing a high tolerance of G. mellonella to these drugs. AML at 15 mg/kg and IMI at 100 mg/kg increased the larvae survival by 20% (p = 0.04) and 11% (p = 0.11), respectively, also positively affecting health score indexes. In agreement with the literature, ciprofloxacin at >100 mg/kg promoted larvae survival by >73%. Conclusions: Amlodipine and imipramine show mild potential as new therapeutic options for managing S. aureus infections but are promising as new lead molecules. This study also reinforces G. mellonella as a sustainable, reliable model for drug evaluation.

Galleria mellonella

Amlodipine

10.3390/antibiotics14020183

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Effects of microcystin-LR, cylindrospermopsin and a microcystin-LR/cylindrospermopsin mixture on growth, oxidative stress and mineral content in lettuce plants (Lactuca sativa L.)

Ecotoxicology and Environmental Safety (2015)

Marisa Freitas Joana Azevedo Edgar Pinto Joana Neves Alexandre Campos

Cylindrospermopsin

Microcystin-LR

Leafy vegetables

10.1016/j.ecoenv.2015.02.002

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Citations (76)

Exposure of Lycopersicon Esculentum to Microcystin-LR: Effects in the Leaf Proteome and Toxin Translocation from Water to Leaves and Fruits

Toxins (2014)

Daniel Gutiérrez‐Praena Alexandre Campos Joana Azevedo Joana Neves Marisa Freitas

Natural toxins such as those produced by freshwater cyanobacteria have been regarded as an emergent environmental threat. However, the impact of these water contaminants in agriculture is not yet fully understood. The aim of this work was to investigate microcystin-LR (MC-LR) toxicity in Lycopersicon esculentum and the toxin accumulation in this horticultural crop. Adult plants (2 month-old) grown in a greenhouse environment were exposed for 2 weeks to either pure MC-LR (100 μg/L) or Microcystis aeruginosa crude extracts containing 100 μg/L MC-LR. Chlorophyll fluorescence was measured, leaf proteome investigated with two-dimensional gel electrophoresis and Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF)/TOF, and toxin bioaccumulation assessed by liquid chromatography-mass spectrometry (LC-MS)/MS. Variations in several protein markers (ATP synthase subunits, Cytochrome b6-f complex iron-sulfur, oxygen-evolving enhancer proteins) highlight the decrease of the capacity of plants to synthesize ATP and to perform photosynthesis, whereas variations in other proteins (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit and ribose-5-phosphate isomerase) suggest an increase of carbon fixation and decrease of carbohydrate metabolism reactions in plants exposed to pure MC-LR and cyanobacterial extracts, respectively. MC-LR was found in roots (1635.21 μg/kg fw), green tomatoes (5.15-5.41 μg/kg fw), mature tomatoes (10.52-10.83 μg/kg fw), and leaves (12,298.18 μg/kg fw). The results raise concerns relative to food safety and point to the necessity of monitoring the bioaccumulation of water toxins in agricultural systems affected by cyanotoxin contamination.

Cylindrospermopsin

Lycopersicon

Microcystin-LR

Proteome

10.3390/toxins6061837

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Citations (61)