Zhao Lei

Kunming Medical University

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Qing Yang

Kunming Medical University

Jian‐Gong Shi

Chinese Academy of Medical Sciences & Peking Union Medical College

Yuming Guo

China Agricultural University

Yuxin Shao

Beijing Academy of Agricultural and Forestry Sciences

Bingkun Zhang

China Agricultural University

Liang YuanCun

Shi Yonglin

Jianmin Yuan

China Agricultural University

Liu Ji-shan

Luye Pharma (China)

Yan Yang

Fujian Medical University

Cooperative Institutions

China Agricultural University

State Key Laboratory of Animal Nutrition

Xi'an Jiaotong University

Peking University

Chinese Academy of Sciences

Beijing Academy of Agricultural and Forestry Sciences

Kunming Medical University

Zhejiang University

University of Chinese Academy of Sciences

Tsinghua University

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Extraction methods and activity of glycosaminoglycan from velvet antler

Zhao Lei

Objective: To compare the effects of different extraction methods on the extraction of glycosaminoglycan from velvet antler, and study the immunomodulatory and antioxidant activity of glycosaminoglycan. Methods: Glycosaminoglycan was extracted from velvet antler by proteasehydrolysis method and dilute alkali-protease hydrolysis method, respectively. Trichloroacetic acid method and Sevage method were used to remove protein from the crude glycosaminoglycan. Basic physical and chemical properties, total glycosaminoglycan, hexuronic acid and protein content were used to evaluate the effect of four extraction methods. The effect of glycosaminoglycan on the proliferation of murine splenocytes was detected by WST-8. Fe 3+ reduction ability, DPPH radical and hydroxyl radical scavenging capacity were used to evaluate the antioxidant activity of glycosaminoglycan from velvet antler. Results: Glycosaminoglycan that extracted by trypsin-papain hydrolysis, following deproteinization with trichloroacetic acid, had the highest purity. The relationship between the concentration of glycosaminoglycan and effect on ConA-stimulated splenocyte proliferation showed an inverted U-shaped curve. Glycosaminoglycan inhibited the proliferation of LPS-stimulated murine splenocytes. Glycosaminoglycan had Fe 3+ reduction ability, DPPH radical and hydroxyl radical scavenging capacity. Conclusions: Protease hydrolysis method is applicable to extract glycosaminoglycan from velvet antler. Glycosaminoglycan from velvet antler has bidirectional immunomodulatory effect and antioxidant activity.

Trichloroacetic acid

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Isolation and Identification of five strains of group I fowl adenovirus serotype 4.

Zhongguo jiaqin (2018)

Zhao Lei Li Lin Shi AiHua Zhang Jianwei Xi Shuo

Isolation

Fowl

Identification

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Metastatic pericardial myxoid liposarcoma: A case report

Asian Journal of Surgery (2022)

Zhao Lei Jian‐Gong Shi Qing Yang

Myxoid liposarcoma

10.1016/j.asjsur.2022.10.111

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Mixed tumor of nasal cavity: A case report

Asian Journal of Surgery (2022)

Jian‐Gong Shi Zhao Lei Qing Yang

10.1016/j.asjsur.2022.09.122

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Protective effect of velvet antler against HCl-ethanol induced gastric mucosal lesions in mice

Zhao Lei

Objective:To study the protective effect of velvet antler against HCl-ethanol induced gastric mucosal lesions in mice.Methods:Ninety mice were randomLy divided into several groups:(1)control group,(2)ranitidine group,(3)glycosaminoglycans group,(4)aqueous extract of velvet antler group(80,200,500 mg/kg bw),(5)velvet antler powder group(80,200,500 mg/kg.bw).At the end of the 5th day of administration,all the animals were treated with 0.2 mL of HCl-ethanol mixture p.o.to induce gastric mucosal lesion.The haemorrhage and damage index of gastric mucosa from each mouse were evaluated.Results:The gastric mucosae of mice in the control group were seriously damaged after treatment of HCl-ethanol.Compared with the control group,administration of glycosaminoglycans and aqueous extract of velvet antler(500 mg/kg.bw) could reduce haemorrhage and damage index of gastric mucosal.Conslusions:It is suggested that various substances contained in velvet antler,especiallyglycosaminoglycans and protein components,could protect gastric mucosa from the damage of H + and ethanol.

Antler

Velvet

Ulcer index

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Experimental observation of the infectivity of three different Trichinella isolates in mice.

Journal of Pathogen Biology (2010)

Yun-ying Yao Xiaoning Chen Xiaodong Yang Zhao ChangQi Bing YuYan

Objective To observe the infectivity of three different geographical isolates of Trichinella spiralis from Heilongjiang,Henan,and Yunnan Provinces in mice. Methods Thirty male Kunming mice were randomly divided into 3 groups (10 mice per group). Each group was orally inoculated with the muscle larvae of trichinella spiralis from one of the three different isolates. Each mouse received 200 motile larvae. The diaphragmatic muscle of the mouse was collected and observed to see if cysts had formed in order to determine when they had formed. The number of larvae in 50 mg of diaphragmatic muscle was counted. Thirty cysts of each isolate were randomly chosen to measure length and width,and then the cyst index of morphology was calculated (length/width). All motile larvae from each mouse were collected and counted. Then,the value of RCI was calculated. The results were compared among the three isolates. Results Cyst formation for the Heilongjiang isolate was 53 days post-infection,which was longer than that for the Henan and Yunnan isolates (40 days post-infection for both). The number of larvae in 50 mg of muscle from mice given the three isolates was 91.8±5.119,301.8±14.839,and 176.4±7.127. There was a significant difference between the cyst index of morphology for the Henan isolate (2.385±0.335) and that of the other two isolates (1.883±0.401,2.360±0.624) (P0.05). The difference between the latter 2 isolates was also significant. The RCI for the Henan isolate was highest (297.500±12.179),followed by the Heilongjiang isolate 66.204±1.569 and Yunnan isolate (154.800±3.894). There was a significant difference among the groups (P0.05). Conclusion The Henan isolate of T. spiralis had a higher level of infectivity than 2 other isolates.

Infectivity

Diaphragm muscle

Trichinella spiralis

Trichinella

Trichinosis

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Zinc Supplementation, via GPR39, Upregulates PKCζ to Protect Intestinal Barrier Integrity in Caco-2 Cells Challenged bySalmonella entericaSerovar Typhimurium

Journal of Nutrition (2017)

Yuxin Shao Zhao Lei Patricia G. Wolf Y Gao Yuming Guo

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