Ryszard Słomski

Institute of Natural Fibres and Medicinal Plants

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Daniel Lipiński

University of Life Sciences in Poznań

Marlena Szalata

University of Life Sciences in Poznań

Andrzej Pławski

Polish Academy of Sciences

Marzena Skrzypczak-Zielińska

Institute of Human Genetics

Joanna Zeyland

University of Life Sciences in Poznań

Karolina Wielgus

Poznan University of Medical Sciences

Agnieszka Dobrowolska

Poznan University of Medical Sciences

Marta Kaczmarek-Ryś

Institute of Human Genetics

Z. Smorąg

National Research Institute of Animal Production

Jolanta Jura

Jagiellonian University

Cooperative Institutions

Poznan University of Medical Sciences

184

Polish Academy of Sciences

122

Institute of Human Genetics

University of Life Sciences in Poznań

Heliodor Swiecicki Clinical Hospital

Adam Mickiewicz University in Poznań

Pomeranian Medical University

Institute of Natural Fibres and Medicinal Plants

Medical University of Silesia

National Research Institute of Animal Production

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Evaluation of the CRISPR/Cas9 Genetic Constructs in Efficient Disruption of Porcine Genes for Xenotransplantation Purposes Along with an Assessment of the Off-Target Mutation Formation

Genes (2020)

Natalia Ryczek Magdalena Hryhorowicz Daniel Lipiński Joanna Zeyland Ryszard Słomski

The increasing life expectancy of humans has led to an increase in the number of patients with chronic diseases and organ failure. However, the imbalance between the supply and the demand for human organs is a serious problem in modern transplantology. One of many solutions to overcome this problem is the use of xenotransplantation. The domestic pig (Sus scrofa domestica) is currently considered as the most suitable for human organ procurement. However, there are discrepancies between pigs and humans that lead to the creation of immunological barriers preventing the direct xenograft. The introduction of appropriate modifications to the pig genome to prevent xenograft rejection is crucial in xenotransplantation studies. In this study, porcine GGTA1, CMAH, β4GalNT2, vWF, ASGR1 genes were selected to introduce genetic modifications. The evaluation of three selected gRNAs within each gene was obtained, which enabled the selection of the best site for efficient introduction of changes. Modifications were examined after nucleofection of porcine primary kidney fibroblasts with CRISPR/Cas9 system genetic constructs, followed by the tracking of indels by decomposition (TIDE) analysis. In addition, off-target analysis was carried out for selected best gRNAs using the TIDE tool, which is new in the research conducted so far and shows the utility of this tool in these studies.

Xenotransplantation

10.3390/genes11060713

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Citations (9)

Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack ) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells

BioTechnologia (2017)

Mariola Galbas Filip Porzucek Marek Selwet Agnieszka Nowak Ryszard Słomski

ENWEndNote BIBJabRef, Mendeley RISPapers, Reference Manager, RefWorks, Zotero AMA E. Galbas M, Porzucek F, Selwet M, Nowak A, Slomski R. Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells. BioTechnologia. 2017;98(3):219-224. doi:10.5114/bta.2017.70800. APA E. Galbas, M., Porzucek, F., Selwet, M., Nowak, A., & Slomski, R. (2017). Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells. BioTechnologia, 98(3), 219-224. https://doi.org/10.5114/bta.2017.70800 Chicago E. Galbas, Mariola, Filip Porzucek, Marek Selwet, Agnieszka Nowak, and Ryszard Slomski. 2017. "Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells". BioTechnologia 98 (3): 219-224. doi:10.5114/bta.2017.70800. Harvard E. Galbas, M., Porzucek, F., Selwet, M., Nowak, A., and Slomski, R. (2017). Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells. BioTechnologia, 98(3), pp.219-224. https://doi.org/10.5114/bta.2017.70800 MLA E. Galbas, Mariola et al. "Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells." BioTechnologia, vol. 98, no. 3, 2017, pp. 219-224. doi:10.5114/bta.2017.70800. Vancouver E. Galbas M, Porzucek F, Selwet M, Nowak A, Slomski R. Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells. BioTechnologia. 2017;98(3):219-224. doi:10.5114/bta.2017.70800.

HeLa

Triticale

10.5114/bta.2017.70800

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Gene polymorphism -418 G/C of tissue inhibitor of metalloproteinases 2 is associated with abdominal aortic aneurysm

Journal of Vascular Surgery (2014)

Joanna Mikołajczyk-Stecyna A. Korcz Marcin Gabriel Katarzyna Pawlaczyk Grzegorz Oszkinis

10.1016/j.jvs.2013.12.045

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Methylation level of HLA-E transgene in the xenogeneic tissues of transgenic pigs

Journal of Molecular and Genetic Medicine (2018)

Marlena Szalata Magdalena Hryhorowicz Agnieszka Nowak Joanna Zeyl Daniel Lipiński Ryszard Słomski

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Analiza powtórzeń tetranukleotydów w DNA

Poznań : Wydawnictwo Akademii Rolniczej im. Augusta Cieszkowskiego eBooks (2001)

Ryszard Słomski Katarzyna Kowalska Marta Kaczmarek-Ryś Jolanta Jura

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Biotechnology in the restoration of extinct animal species. An analysis of genomic and mitochondrial DNA of aurochs

BioTechnologia (2011)

Daniel Lipiński Hanna Przystałowska Marlena Szalata Joanna Zeyland Karolina Wielgus

Ancient DNA

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Citations (1)

[Immunogenic RNA in the treatment of neoplasms].

PubMed (1978)

Latos Am Ryszard Słomski

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Impact of CYP2E1, GSTA1 and GSTP1 gene variants on serum alpha glutathione S-transferase level in patients undergoing anaesthesia

BMC Medical Genetics (2016)

Adam Mikstacki Marzena Skrzypczak-Zielińska Oliwia Zakerska‐Banaszak Barbara Tamowicz Maria Skibińska

The serum glutathione S-transferase alpha (α-GST) concentration has been used as a marker of hepatic condition. After sevoflurane anaesthesia a mild impairment of hepatocellular integrity was observed. Genetic polymorphisms in CYP2E1, GSTA1 and GSTP1 genes, affecting enzymes activity, may possibly influence the hepatotoxic effect of sevoflurane. The aim of this study was to assess the influence of genetic polymorphism of CYP2E1, GSTA1 and GSTP1 genes on serum α-GST level in 86 unrelated patients representing ASA physical status I-II, undergoing laryngological surgery under general anaesthesia with sevoflurane. The serum samples from three perioperative time points were analyzed using ELISA. Genetic variants were detected by pyrosequencing and sequencing. Finally, the statistical associations between serum α-GST concentration and analyzed alleles of CYP2E1, GSTP1 and GSTA1 genes were estimated. The allele GSTA1*B (−567G, −69T, −52A) frequency was 0.43, whereas the alleles c.313G and c.341T of GSTP1 were identified with frequencies of 0.28 and 0.1 respectively. The -1053T allele of the CYP2E1 gene was observed with 0.01 frequency. We found serum α-GST concentrations in homozygous changes c.313A>G and c.341C>T of the GSTP1 gene significantly higher at the end of anaesthesia as compared with the levels at pre-anaesthetic and 24 h post-anaesthetic time points. Moreover, GSTA1 wild type genotype was associated with increased α-GST concentration at 24 h after the end of anaesthesia. GSTP1 gene polymorphism has an impact on the perioperative serum α-GST concentration in patients undergoing sevoflurane anaesthesia. A similar association, although not statistically significant exists between GSTA1 gene variants and perioperative serum α-GST level.

GSTP1

Glutathione S-transferase

10.1186/s12881-016-0302-6

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Citations (15)

[Use of polymerase chain reaction (PCR) in clinical diagnosis].

PubMed (1992)

Ryszard Słomski H. Chlebowska Jochen Reiss

Hot start PCR

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