Mel V. Boo

National University of Singapore

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Yuen K. Ip

National University of Singapore

Shit F. Chew

Nanyang Technological University

Wai P. Wong

Monash University Malaysia

Kum C. Hiong

National Parks Board

Celine Y. L. Choo

National Chung Hsing University

Jeslyn S. T. Poo

National University of Singapore

Mei Lin Neo

National University of Singapore

Biyun Ching

National University of Singapore

Pham Hong Anh Cao

The University of Texas MD Anderson Cancer Center

Clarissa Z. Y. Koh

National University of Singapore

Cooperative Institutions

National University of Singapore

Nanyang Technological University

National Institute of Education Sciences

National Institute of Education

University of Ottawa

The University of Texas MD Anderson Cancer Center

The University of Texas Health Science Center at Houston

Valent BioSciences (United States)

Duke-NUS Medical School

University of North Carolina at Chapel Hill

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Molecular characterization, light-dependent expression, and cellular localization of a host vacuolar-type H + -ATPase (VHA) subunit A in the giant clam, Tridacna squamosa , indicate the involvement of the host VHA in the uptake of inorganic carbon and its supply to the symbiotic zooxanthellae

Gene (2018)

Yuen K. Ip Kum C. Hiong Leon Jian Ying Lim Celine Y. L. Choo Mel V. Boo

Zooxanthellae

10.1016/j.gene.2018.03.054

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Citations (37)

Light-enhanced phosphate absorption in the fluted giant clam, Tridacna squamosa, entails an increase in the expression of sodium-dependent phosphate transporter 2a in its colourful outer mantle

Coral Reefs (2020)

Christabel Y. L. Chan Kum C. Hiong Celine Y. L. Choo Mel V. Boo Wai Peng Wong

10.1007/s00338-020-01930-w

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Citations (11)

Dataset for: Light-dependent expression of a Na+/H+ exchanger 3-like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light-enhanced calcification

Figshare (2017)

Kum C. Hiong Pham Hong Anh Cao Celine Y. L. Choo Mel V. Boo Wai Peng Wong

Na+/H+ exchangers (NHEs) regulate intracellular pH and ionic balance by mediating H+ efflux in exchange for Na+ uptake in a 1:1 stoichiometry. This study aimed to obtain from the ctenidium of the giant clam Tridacna squamosa (TS) the complete cDNA sequence of a NHE3-like transporter (TSNHE3), and to determine the effect of light exposure on its mRNA expression level and protein abundance therein. The coding sequence of TSNHE3 comprised 2886 bp, encoding 961 amino acids with an estimated molecular mass of 105.7 kDa. Immunofluorescence microscopy revealed that TSNHE3 was localized to the apical membrane of epithelial cells of the ctenidial filaments and the tertiary water channels. Particularly, the apical immunofluorescence of the ctenidial filaments was consistently stronger in the ctenidium of clams exposed to 12 h of light than those of the control kept in darkness. Indeed, light induced significant increases in the transcript level and protein abundance of TSNHE3/TSNHE3 in the ctenidium, indicating that the transcription and translation of TSNHE3/TSNHE3 were light-dependent. As light-enhanced calcification generates H+, the increased expression of TSNHE3/TSNHE3 in the ctenidium could be a response to augment H+ excretion in pursuance of whole-body acid-base balance during light exposure. These results signify that shell formation in giant clams requires the collaboration between the ctenidium, which is a respiratory and iono-regulatory organ, and the inner mantle, which is directly involved in the calcification process, and provide new insights into the mechanisms of light-enhanced calcification in giant clams.

10.6084/m9.figshare.4818373

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The colorful mantle of the giant clam, Tridacna squamosa, expresses a light-dependent manganese superoxide dismutase to ameliorate oxidative stresses due to its symbiotic association with zooxanthellae

Coral Reefs (2018)

Kum C. Hiong Clarissa Z. Y. Koh Mel V. Boo Celine Y. L. Choo Wai Peng Wong

Zooxanthellae

10.1007/s00338-018-01738-9

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Citations (10)

Dataset for: Carbonic Anhydrase 2-like in the giant clam, Tridacna squamosa: characterization, localization, response to light, and possible role in the transport of inorganic carbon from the host to its symbionts

Figshare (2017)

Yuen K. Ip Clarissa Z. Y. Koh Kum C. Hiong Celine Y. L. Choo Mel V. Boo

The fluted giant clam, Tridacna squamosa, lives in symbiosis with zooxanthellae which reside extracellularly inside a tubular system. Zooxanthellae fix inorganic carbon (C_i) during insolation and donate photosynthate to the host. Carbonic anhydrases catalyze the interconversion of CO₂ and HCO₃^-, of which carbonic anhydrase 2 (CA2) is the most ubiquitous and involved in many biological processes. This study aimed to clone a CA2 homolog (CA2-like) from the fleshy and colorful outer mantle as well as the thin and whitish inner mantle of T. squamosa, to determine its cellular and subcellular localization, and to examine the effects of light exposure on its gene and protein expression levels. The cDNA coding sequence of CA2-like from T. squamosa comprised 789 bp, encoding 263 amino acids with an estimated molecular mass of 29.6 kDa. A phenogramic analysis of the deduced CA2-like sequence denoted an animal origin. CA2-like was not detectable in the shell-facing epithelium of the inner mantle adjacent to the extrapallial fluid. Hence, CA2-like is unlikely to participate directly in light-enhanced calcification. By contrast, the outer mantle, which contains the highest density of tertiary tubules and zooxanthellae, displayed high level of CA2-like expression, and CA2-like was localized to the tubule epithelial cells. More importantly, exposure to light induced significant increases in the protein abundance of CA2-like in the outer mantle. Hence, CA2-like could probably take part in the increased supply of inorganic carbon (C_i) from the host clam to the symbiotic zooxanthellae when the latter conduct photosynthesis to fix C_i during light exposure.

Characterization

10.6084/m9.figshare.5513500

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The giant clam Tridacna squamosa quickly regenerates iridocytes and restores symbiont quantity and phototrophic potential to above-control levels in the outer mantle after darkness-induced bleaching

Coral Reefs (2021)

Yuen K. Ip Mel V. Boo Miguel Mies Shit F. Chew

Darkness

10.1007/s00338-021-02199-3

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Citations (6)

Molecular Characterization of a Dual Domain Carbonic Anhydrase From the Ctenidium of the Giant Clam, Tridacna squamosa, and Its Expression Levels After Light Exposure, Cellular Localization, and Possible Role in the Uptake of Exogenous Inorganic Carbon

Frontiers in Physiology (2018)

Clarissa Z. Y. Koh Kum C. Hiong Celine Y. L. Choo Mel V. Boo Wai Peng Wong

A Dual-Domain Carbonic Anhydrase (DDCA) had been sequenced and characterized from the ctenidia (gills) of the giant clam, Tridacna squamosa, which lives in symbiosis with zooxanthellae. DDCA was expressed predominantly in the ctenidium. The complete cDNA coding sequence of DDCA from T. squamosa comprised 1,803 bp, encoding a protein of 601 amino acids and 66.7 kDa. The deduced DDCA sequence contained two distinct α-CA domains, each with a specific catalytic site. It had a high sequence similarity with tgCA from Tridacna gigas. In T. squamosa, the DDCA was localized apically in certain epithelial cells near the base of the ctenidial filament and the epithelial cells surrounding the tertiary water channels. Due to the presence of two transmembrane regions in the DDCA, one of the Zn2+-containing active sites could be located externally and the other one inside the cell. These results denote that the ctenidial DDCA was positioned to dehydrate HCO3− to CO2 in seawater, and to hydrate the CO2 that had permeated the apical membrane back to HCO3− in the cytoplasm. During insolation, the host clam needs to increase the uptake of inorganic carbon from the ambient seawater to benefit the symbiotic zooxanthellae; only then, can the symbionts conduct photosynthesis and share the photosynthates with the host. Indeed, the transcript and protein levels of DDCA/DDCA in the ctenidium of T. squamosa increased significantly after 6 and 12 h of exposure to light, respectively, denoting that DDCA could participate in the light-enhanced uptake and assimilation of exogenous inorganic carbon.

Zooxanthellae

10.3389/fphys.2018.00281

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Citations (29)

Evidence for the involvement of branchial Vacuolar-type H+-ATPase in the acidification of the external medium by the West African lungfish, Protopterus annectens, exposed to ammonia-loading conditions

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology (2022)

Yuen K. Ip Charmaine W Q Leong Mel V. Boo Wai Peng Wong Siew Hong Lam

10.1016/j.cbpa.2022.111297

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Citations (3)

The fluted giant clam (Tridacna squamosa) increases the protein abundance of the host's copper-zinc superoxide dismutase in the colorful outer mantle, but not the whitish inner mantle, during light exposure

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology (2020)