Autoimmune processes have been implicated in the development of rheumatoid arthritis (RA); however, specific autoantigens that play a role in the aetiology of RA have been lacking. In this study, we found that sera from RA patients were particularly immunoreactive against the protein tryptase. Compared with osteoarthritis (OA) patients and healthy controls, RA patients had relatively higher levels of tryptase and concomitant anti-tryptase antibodies in their synovial tissues and sera. Similarly, synovial fluid from RA patients, but not from OA patients, contained antibodies that recognized tryptase in vitro. In addition, serum tryptase levels in both early and late RA patients significantly correlated with clinical indices usually used to diagnose RA, such as rheumatoid factor, Disease Activity Score using 28 joint counts and autoantibodies against cyclic citrullinated peptide. Our results identify tryptase as a candidate autoantigen involved in the pathogenesis of RA and monitoring its levels may have diagnostic and prognostic value.
Gastric cancer (GC) remains poor prognosis and survival issues due to the resistance of chemotherapies, such as cisplatin. The long non-coding RNA small nucleolar RNA host gene 7 (lncRNA-SNHG7) is known as an oncogenic molecule in diverse cancers. Here, we demonstrate that SNHG7 was significantly upregulated in gastric cancer and positively correlated with cisplatin resistance of gastric cancer cells that SNHG7 was significantly upregulated in cisplatin resistant cells. Silencing SNHG7 dramatically sensitized cisplatin resistant cells. In contrast, a negative correlation between lncRNA-SNHG7 and miR-34a was found that miR-34a was downregulated in gastric cancer patient tissues and significantly sensitized cisplatin resistant gastric cancer cells. Intriguingly, bioinformatical analysis indicated miR-34a has putative biding site for SNHG7 and such negative association between SNHG7 and miR-34a was verified in gastric cancer tissues. The cisplatin resistant cells displayed increased glycolysis rate and SNHG7 promoted cellular glycolysis rate of gastric cancer cells. Luciferase assay illustrated LDHA, a glycolysis enzyme, was the direct target of miR-34a. Importantly, inhibiting SNHG7 successfully suppressed LDHA expressions and sensitized cisplatin resistant cells and such inhibitory effects could be recovered by further anti-miR-34a. These findings suggest an important regulator mechanism for the SNHG7-mediated cisplatin resistance via miR-34a/LDHA-glycolysis axis.
Objective
To explore the effects of different exercise modes of ball-holding on preventing peripherally inserted central catheter (PICC) related thrombus, so as to provide a basis for formulating scientific nursing intervention.
Methods
From June to November 2017, this study selected 86 cancer patients with chemotherapy through PICC of Oncology Department in First Affiliated Hospital of PLA General Hospital by purposive sampling. All of the patients from two wards of Oncology Department were divided into the experimental group and the control group by tossing a coin. Patients of the experimental group carried out ball-holding 25 times to 30 times after 3 meals each day with clenching for 10 seconds, loosening 10 seconds and grip strength according to that the ball was compressed to one half. Patients of the control group accepted conventional nursing that ball-holding was implemented 3 times, a total of 500 times. Color Doppler ultrasound was used to dynamically observe the blood flow velocity, incidence, formation position, formation time and classification of thrombus as well as other complications after indwelling catheter. The SAS 9.4 was used to statistical analysis.
Results
This study involved 42 patients in the experimental group and 41 patients in the control group after removing missing patients. The maximum velocity of axillary vein of patients in experimental group before and 1, 3, 7, 14, 21, 28 days after indwelling catheter was (16.12±3.62) , (15.83±3.07) , (15.69±3.01) , (15.05±2.49) , (14.46±3.85) , (15.47±2.95) and (14.75±2.91) cm/s respectively. There was no significant difference in blood flow velocity before indwelling catheter between two groups (t=-0.54, P=0.594 5) . But, there were significant differences in blood flow velocity 21 and 28 days after indwelling catheter between two groups (t=-5.00, -4.69; P<0.000 1) . The difference of the maximum velocity in interaction between groups and different time points was significant (F=22.23, P<0.000 1) . The average velocity per unit time of axillary vein of experimental group before indwelling catheter and 1, 3, 7, 14, 21, 28 days after indwelling catheter was (9.97±3.01) , (9.69±2.61) , (9.72±2.49) , (8.88±1.47) , (8.55±2.20) , (9.48±2.71) , (9.26±2.73) cm/s respectively. There was no significant difference in average velocity before indwelling catheter between two groups (t=0.07, P=0.945 9) . After indwelling catheter, there were significant differences in average velocity 21 and 28 days after indwelling catheter between two groups (t=-4.60, -4.48; P<0.000 1) . There was also a significant difference in average velocity per unit time of axillary vein among the interaction between groups and different points (F=9.84, P<0.000 1) . The flow velocity of axillary vein of two groups declined over time 14 days before indwelling catheter. The flow velocity of experimental group gradually rose over time 14 days after indwelling catheter and was with a fall after rise 21 days after reaching peak value; the flow velocity of control group remarkably slowed over time. A significant difference was found in incidence of catheter related thrombus between two groups (P<0.05) . The incidence of thrombus of control group constantly rose over time (χ2=6.158, P=0.013) . There was also significant difference in incidence of other catheter related thrombus complications between two groups (χ2=5.760, P=0.016) .
Conclusions
The modes of ball-holding of experimental group can make the flow velocity of axillary vein in a high level and be propitious to prevent blood stagnation, reduce the incidences of thrombus and other catheter related thrombus complications. It is worth to be used in clinical application.
Key words:
Nursing care; Venous thrombosis; Ball-holding sport; Peripherally inserted central catheter; Catheter-related thrombus; Venous flow velocity
An adverse intrauterine environment impairs the development of pancreatic islets in the fetus and leads to insufficient β cell mass and β cell dysfunction. We previously reported that Pex14, a peroxin protein involved in the biogenesis and degradation of peroxisomes, is markedly reduced in the pancreas of an intrauterine growth restriction fetus and last into adulthood. Peroxisomes function in a wide range of metabolic processes including fatty acid oxidization, ROS detoxification, and anti‐inflammatory responses. To elucidate the impact of downregulation of the Pex14 gene on β cell, Pex14 was knocked down by siRNA in INS‐1 cells. Pex14 knockdown disturbed peroxisomal biogenesis and dysregulated fatty acid metabolism and lipid storage capability, thereby increased ROS level and blunted insulin secretion. Moreover, Pex14 knockdown upregulated inflammation factors and regulators of endoplasmic reticulum stress. The lipotoxicity of fatty acid (including palmitic acid and linoleic acid) in β cells was exacerbated by knockdown of Pex14, as indicated by H 2 O 2 accumulation and increased programmed cell death. The present results demonstrate the vital role of Pex14 in maintaining normal peroxisome function and β cell viability and highlight the importance of a functional peroxisomal metabolism for the detoxification of excess FAs in β cells.
The relatively low direct mortality caused by Paranosema locustae (Canning) has limited its application for controlling grasshopper when densities are high, and this study sought to determine if the simultaneous use of this pathogen and the IGR, Flufenoxuron (Cascade) could provide effective control. Nine treatments were tested: 45% Malathion EC at 1500 ml/ha, 5% Cascade at 150 ml/ha, 5% Cascade at 75 ml/ha, 5% Cascade at 37.5 ml/ha, P. locustae at 7.5 × 109 spores/ha, combinations of 5% Cascade at 75 ml/ha and P. locustae at 7.5 × 109 spores/ha, applied in different rations (1:1, 1:2, 1:3) in the same plot, the untreated control. P. locustae was applied on nonoverlapping plots with the IGR. The different in-plot combinations of P. locustae and Cascade in different ratios provided significantly better overall control of grasshoppers (all species) than the treatment of 5% Cascade of 150 ml/ha after 5d, but combinations were not significantly different from the other concentrations of Cascade after 12 and 31 d. When results were examined separately for specific species of grasshoppers, reduction of Dasyhippus harbipes (Fischer-Waldheim), was higher than that of Myrmeleotettix palpalis (Zubovsky). While combinations showed significant differences in the infection of different grasshopper species at 5 and 12 d posttreatment, no significant differences in rate of infection among the primary species (M. palpalis, D. harbipes, and Oedaleus asiaticus Bei-Bienko) were detected 31 d posttreatment. Our study found that P. locustae by itself could control grasshopper populations at medium densities but the combined application of P. locustae and Cascade at a ratio of 1:2 was more effective against high-density grasshopper populations.
Abstract The advance in nursing care for stoma patients is a challenging issue, which will influence the life quality. The quality of life is a major issue in the recovery of stoma patients. The evidence of experimental nursing has not been explored enough. A systematic search and a meta‐analysis were performed for the studies of experimental nursing interventions versus routine warming interventions on patients with a stoma. The comparisons between nursing interventions were performed to find which kind of intervention will be superior in improving life quality. After a restricted selection, 10 studies, 460 subjects with experimental nursing intervention, and 478 controls with the routine nursing intervention were enrolled in a variety of causes of the stoma. The focused outcome was the quality of life. The meta‐analysis was performed by Review Manager 5.4. Among the stoma patients, the meta‐analysis favours the experimental nursing intervention group with higher scores of life quality when compared to the routine nursing intervention group. The meta‐analysis results were with positive mean differences, significant tests for overall effect, and significant heterogeneities in the random‐effects model. The experimental nursing intervention showed higher positive effects on the quality of life when compared to routine nursing intervention for stoma patients. Experimental nursing intervention might be an option for stoma nursing practitioners to improve stoma care.
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