Shuifu Chen

Zhejiang University

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Yao‐Guang Liu

South China Agricultural University

Yuanling Chen

Sanya University

Letian Chen

South China Agricultural University

Xiucai Zhao

South China Agricultural University

Qinlong Zhu

South China Agricultural University

Sun Bing-nan

Shenyang University of Chemical Technology

Xianrong Xie

South China Agricultural University

Yuchao Xia

Chongqing Jiaotong University

Gregory A. Kopp

Western University

Jingluan Han

South China Agricultural University

Cooperative Institutions

South China Agricultural University

State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources

Key Laboratory of Guangdong Province

Zhejiang University

Chinese Academy of Sciences

Nanchang University

Central South University

Chinese Academy of Agricultural Sciences

Institute of Subtropical Agriculture

Tongji University

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An asymmetric allelic interaction drives allele transmission bias in interspecific rice hybrids

Nature Communications (2019)

Yongyao Xie Jintao Tang Xianrong Xie Xiaojuan Li Jianle Huang

Abstract Hybrid sterility (HS) between Oryza sativa (Asian rice) and O . glaberrima (African rice) is mainly controlled by the S1 locus. However, our limited understanding of the HS mechanism hampers utilization of the strong interspecific heterosis. Here, we show that three closely linked genes ( S1A4 , S1TPR , and S1A6 ) in the African S1 allele ( S1-g ) constitute a killer-protector system that eliminates gametes carrying the Asian allele ( S1-s ). In Asian–African rice hybrids ( S1-gS1-s ), the S1TPR-S1A4-S1A6 interaction in sporophytic tissues generates an abortion signal to male and female gametes. However, S1TPR can rescue S1-g gametes, while the S1-s gametes selectively abort for lacking S1TPR. Knockout of any of the S1-g genes eliminates the HS. Evolutionary analysis suggests that S1 may have arisen from newly evolved genes, multi-step recombination, and nucleotide variations. Our findings will help to overcome the interspecific reproductive barrier and use Asian–African hybrids for increasing rice production.

Oryza

10.1038/s41467-019-10488-3

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Citations (62)

Wind tunnel experimental study of Wind pressure distribution on tall buildings considering surrounding interference

Zheng Yongxin Shuifu Chen

Wind loads on buildings in realistic environments may be considerably different from those measured on isolated buildings. Neighboring structures may either decrease or increase the flow-induced forces on the building being considered. In this paper a rigid model wind tunnel test under conventional terrain C wind field was performed to the 1:250 reduced-scale model of a high-rise building with three surrounding buildings for investigating the interference effects of wind-pressure distribution. The wind pressure coefficients under 8 wind directions were measured for the isolated building and the building with three other surroundings. Based on the test data, a detailed comparative study was conducted towards the wind pressure distributions on the building with and without surrounding others. Essential conclusions are as follows: the upstream building can decrease the wind load on downstream building. The shielding effect is related to the height of the disturbing buildings. Wind pressure distributions on the building with and without disturbing buildings tend to be similar above the height of the disturbing buildings.

Wind engineering

Pressure measurement

10.1109/cecnet.2011.5769416

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Citations (9)

ULTIMATE LOAD-CAPACITY ANALYSIS OF LIGHT-WEIGHT STEEL PORTAL FRAMES UNDER WIND AND SNOW LOADING

Low Temperature Architecture Technology (2013)

Shuifu Chen

Nonlinear ultimate limit state analysis was performed to a typical frame of an existing light-weight steel portal frame industrial building located at the coastal region of China using the refined finite element method.The wind and snow load combinations were determined from the current load code of China,and the corresponding wind-resistant and snow-resistant load-capacity redundant factors of the frame were then found through the analysis.The obtained results indicate that although the load-capacities of the frame under the two load combinations meet the current code requirement,the load-capacity redundant factors are relatively low if initial imperfections of the frame are considered.Furthermore the failure mode of the frame would change from a material yielding format to a local buckling format under consideration of initial imperfections.

Wind engineering

Portal frame

Limit state design

Mode (computer interface)

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Analysis and Evaluation of Normal Sectional Load Carrying Capacity of Steel Reinforced Concrete Columns

Jiegou Gongchengshi (2004)

Shuifu Chen

An accurate iterative computer-method for determination of normal sectional load carrying capacity of steel reinforced concete composite columns is presented in this paper, which can be used to analyze the arbitrarily shaped composite column. Based on this, some examples are used to evaluate the simplified methods recommendded by the Technical Specification for Steel Reinforced Concrete Composite Structures. Then, several useful conclusions and suggestions for the design of this type of columns are recommended.

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An Efficient Marker Gene Excision Strategy Based on CRISPR/Cas9-Mediated Homology-Directed Repair in Rice

International Journal of Molecular Sciences (2022)

Jiantao Tan Yaxi Wang Shuifu Chen Zhansheng Lin Yanchang Zhao

In order to separate transformed cells from non-transformed cells, antibiotic selectable marker genes are usually utilized in genetic transformation. After obtaining transgenic plants, it is often necessary to remove the marker gene from the plant genome in order to avoid regulatory issues. However, many marker-free systems are time-consuming and labor-intensive. Homology-directed repair (HDR) is a process of homologous recombination using homologous arms for efficient and precise repair of DNA double-strand breaks (DSBs). The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) system is a powerful genome editing tool that can efficiently cause DSBs. Here, we isolated a rice promoter (P

Selectable marker

Marker gene

Homology

10.3390/ijms23031588

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Citations (7)

Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants

Science China Life Sciences (2020)

Hao Yu Wubei Zong Dongchang Zeng Jingluan Han Shuifu Chen

Multiplex

10.1007/s11427-019-1612-6

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Citations (17)

Practical Analysis of Wind-Induced Human Comfort Condition of Tall Buildings

International Conference on E-Product E-Service and E-Entertainment (2010)

Shuifu Chen Chao Li

A practical analysis procedure for estimation of the wind-induced root mean square acceleration response and the human comfort condition of tall buildings is presented in this paper. The modal wind-force time history curves and the corresponding power spectrum curves are first determined through analysis of the wind-pressure coefficient data obtained from the aerodynamic wind-tunnel model test and the dynamic characteristics analysis of the building structure. The relationship of modal force power spectrum to natural frequency of the structure can then be found by using the curve-fitting method. Based on the random vibration theory a simplified expression of the root mean square acceleration with respect to the natural frequency considering the resonance response of the structure can be established. By comparing the obtained expression with the acceleration limits related to the human comfort requirement, the wind-induced human comfort condition assessment may be achieved.

Root mean square

Natural frequency

Response spectrum

10.1109/iceee.2010.5660343

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Analysis and practical determination of wind-load shape factors for low-rise hip roofed buildings

Jisuan lixue xuebao (2007)

Shuifu Chen

Practical formulas for determination of wind-load shape factors on the roof of low-rise hip roofed buildings under different wind directions have been proposed by means of the numerical simulation and wind tunnel test investigation.The basic size of the building under consideration is 7.5 m×15 m in plan,9.6 m of eave height and 1.5 m of overhang length.A boundary layer wind tunnel test was conducted for the building model at a geometric scale of 1∶20 and numerical simulations were carried out for the buildings with various roof pitches of 15°,25°,35°45°and 60°,various height-width ratios of 0.4,1.0,2.0,3.0 and 4.0 and various length-width ratios of 2.0,3.0,4.0,5.0 and 6.0.The obtained numerical results and the corresponding test data(if available) are presented in the paper and compared with each other.The results indicate that the roof pitch and height-width ratio are the main factors influencing the hip roof pressures.Practical formulas for determination of the hip roof shape factors are then proposed.A numerical example showing application of these formulas is finally given and good agreement is reached between the practical determination and wind tunnel test.It demonstrates that the proposed formulas are accurate and directly applicable to wind resistant design of hip roofed buildings.

Eaves

Wind engineering

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NUMERICAL SIMULATION OF WIND PRESSURES ON CANTILEVER GRANDSTAND ROOFS

Engineering Mechanics (2007)

Shuifu Chen

Numerical simulation has been carried out for the prediction of wind pressure distributions on cantilever grandstand roofs. In order to demonstrate the validity and accuracy of the numerical simulation technique, a flat grandstand roof with wind-tunnel test pressure results was first considered. The computed roof surface pressures were compared with the corresponding wind-tunnel test results and overall good agreement was reached. The wind pressure distribution on a real cantilevered grandstand roof located in Zhejiang Province of China was then examined. Two different cases with 0° and 16° roof slopes were taken into account. Through analysis and comparison of the computed wind pressure results for the two most unfavorable wind directions, 0° and 180°, a simplified pressure distribution curve and the corresponding pressure determination method for this type of roof structures were proposed. The distribution curve was also compared with those recommended by relevant design codes. This simplified pressure determination method is directly applicable to practical wind resistant design of same type of cantilever roof structures.

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Effects of plan dimensions on gust wind loads for high-rise buildings

Journal of Wind Engineering and Industrial Aerodynamics (2019)

Yi Liu Gregory A. Kopp Shuifu Chen

Load factor

Wind engineering

10.1016/j.jweia.2019.103980

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Citations (18)