The objective of this work was to evaluate the development, physiology and resistance to downy mildew of the BRS Margot grapevine treated with biodynamic preparations in a greenhouse. The experimental design was completely randomized with three treatments and ten replications in each treatment. The treatments were: 1) control pots with application of water in the aerial part; 2) organic treatment: application of chitosan (Fish Fertil® 200 mL.100 L-1) in the aerial part; 3) biodynamic treatment: application of biodynamic formulation silica horn (501) (4 g.60 L-1) in the aerial part and compost with formulations 502 to 507. Four leaf applications of the water, chitosan and preparation (501) were carried out in the 8 and 14 leaves separated stages in January and February (first cycle), November and December 2015 (second cycle) and two application of compost in plantation and September 2015 in the wooly bud stage. The plants were evaluated for the length and diameter of the canes, trunk, SPAD index, gas exchange and area under the disease progress curve (AUDPC). Biodynamic formulations had an effect on the development of the BRS Margot grapevine, verified by the increase of the length of the branches, CO2 assimilation, RuBisCO enzyme efficiency, chlorophyll content (SPAD index) and reduced the incidence of mildew.

10.19084/rca.17180

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True colors shining through: how low tunnel cover colors affect fruit yield and photosynthesis in strawberry cultivars

Vegetos (2024)

Juliane Maciel Henschel Juliano Tadeu Vilela de Resende André Ricardo Zeist Regina Lopes dos Santos Vanderlei Aparecido de Lima

Affect

10.1007/s42535-024-00985-2

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Control of the fungi Lasiodiplodia theobromae, the causal agent of dieback, in cv. syrah grapevines

Figshare (2021)

Carine Rusin F. R. Cavalcanti Patrícia Carla Giloni-Lima Cacilda Márcia Duarte Rios Faria Marcus André Kurtz Almança

ABSTRACT. Grapevine trunk diseases are among the most important limiting factors of worldwide viticulture. In this context, we aimed to verify the effect of chemical fungicides, biological agents and plant extracts on the control of Lasiodiplodia theobromae in pruning wounds and their physiological effects in cv. Syrah grapevines. Plant extracts (clove, cinnamon, garlic, rosemary and lemongrass), commercial fungicides (difeconazole, tebuconazole, mancozeb, sulfur, pyraclostrobin, fosetyl-Al, and azoxystrobin), chitosan, Trichoderma harzianum and Bacillus subtilis were used for the in vitro trials. The protection of pruning wounds in the potted vines was studied using fosetyl-Al, tebuconazole, Trichoderma harzianum, Bacillus subtilis, garlic extract and clove extract. The experiments were carried out through two vegetative cycles: 2015/2016 and 2016/2017. The length of wood discoloration, pathogen re-isolation percentage, fresh mass of the pruning material, peroxidase activity, fluorescence, chlorophyll index, phenological stages, shoot length and leaf area were evaluated. The treatments with clove, garlic extract, tebuconazole, pyraclostrobin, mancozeb, fosetyl-Al and B. subtilis reduced mycelial growth by more than 90%. In the grapevines, the use of T. harzianum decreased the re-isolation of L. theobromae, but no differences were verified for the other plant evaluations. We concluded that the use of T. harzianum would be a potential option for wound protection without altering the physiological aspects of cv. Syrah grapevines.

Lasiodiplodia theobromae

10.6084/m9.figshare.14288169

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Agronomic, Physiological, and Post-Harvest Aspects of Different Blueberry Cultivars Treated with Silicon

Comunicata Scientiae (2023)

Thays Silva Renan Caldas Umburanas Carla García Gabriela Datsch Bennemann Patrícia Carla Giloni-Lima

The objective of this study was to evaluate the effect of foliar application of silicon in different blueberry (Vaccinium myrtillus) cultivars. The treatments were installed in a factorial design with six replications, and consisted of three different cultivars (Brightwell, Beckblue and Climax) with or without regular application of silicon via foliar, using silicon oxide at 1.5 g L-1. It was evaluated the following plant attributes: leaf area, chlorophyll fluorescence, and phenylalanine ammonia lyase activity, meanwhile the following postharvest attributes were evaluated: fruit number, diameter and weight, yield, pH, content of soluble solids, titratable acidity, contents of phenolic compounds and anthocyanins, and activity of peroxidase. Vegetative development of blueberry plants in response to Si presented higher yield, leaf area, phenylalanine ammonia lyase activity, and higher chlorophyll fluorescence relationships such as maximum photochemical efficiency and PSII potential activity, as well as less stressed plants, especially in cultivars Climax and Brightwell. For post-harvest physicochemical characteristics, silicon foliar application improved fruit weight and diameter for cvs. Beckblue and Climax, and fruit number for cv. Brightwell. All cultivars, in response to silicon application increased the titratable acidity, the levels of phenolic compounds, the level of anthocyanins, whereas peroxidase activity decreased. The performance of the evaluated blueberry cultivars was better through silicon application, with greater performance of vegetative growth, fruit yield, and fruit quality, especially in cultivar Climax, which was the most suitable in terms in of fruit yield in this study.

Titratable acid

Vaccinium

Phenylalanine ammonia-lyase

10.14295/cs.v14.4043

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The Use of Chlorophyll a fluorescence as a Marker for the Stress Detection in situ Bioassays with Limnobium laevigatum

Ensaios e Ciência C Biológicas Agrárias e da Saúde (2022)

Rhuann Carlo Viero Taques Vanderlei Aparecido de Lima Gabriela Pupo Hagemeyer Patrícia Carla Giloni-Lima

The aim of this study was to evaluate the use of the Fluorescence of chlorophyll a (FChla) as a marker for stress detection in bioassays with Limnobium laevigatum. Therefore, three tests were carried out: 1) in the laboratory; 2) in situ in a polluted aquatic environment; 3) in situ in a preserved aquatic environment. To diagnose the environmental status of aquatic environments, physical and chemical parameters were measured using a Multiparameter probe (Hanna, Hi 9828). FChla was measured and calculated using the PAM-2500 Fluorometer (Pulse Amplitud Modulated, Heinz Walz, GmB) and the JIP test. The chlorophyll a extraction and analysis was carried out according to the methodology proposed by Porra (2002). The data set was submitted to statistical analysis by generalized linear models (GLM) with normal and gamma distribution with functions of Identity and Log binding. The chlorophyll a fluorescence using the JIP test parameters was able to detect changes in the physiological conditions of the macrophyte Limnobium laevigatum after in situ ecotoxicological assays in preserved and anthropized aquatic environments. This type of spectroscopy can be used as a marker to indicate degrees of pollution in aquatic environments. It was that L. laevigatum, a species not conventionally used yet in bioassays in the field of aquatic ecotoxicology, is sensitive to changes in environmental conditions. Keywords: OJIP Test. Energy Dissipation. Photosynthesis. Macrophyte. Resumo O objetivo deste estudo foi avaliar o uso da Fluorescência da clorofila (FChla) a como marcador para detecção de estresse em bioensaios com Limnobium laevigatum. Para tanto, foram realizados três ensaios: 1) em laboratório; 2) in situ em um ambiente aquático poluído; 3) in situ em um ambiente aquático preservado. Para diagnosticar o status ambiental dos meios aquáticos foram mensurados parâmetros físicos e químicos por meio de sonda Multiparâmetros (Hanna, Hi 9828). A FChla foi mensurada por meio do Fluorômetro PAM-2500 (Pulse Amplitud Modulated, Heinz Walz, GmB) e do JIP teste. A extração e análise de clorofila a foi realizada segundo a metodologia proposta por Porra (2002). O conjunto de dados foi submetido a análise estatística pelos modelos lineares generalizados (MLG) com distribuição normal e gama com funções de ligação Identidade e Log. A fluorescência da clorofila a por meio dos parâmetros do JIP teste foi capaz de detectar mudanças nas condições fisiológicas da macrófita Limnobium laevigatum após os ensaios ecotoxicológicos in situ em ambientes aquáticos preservados e antropizados. Esse tipo de espectroscopia pode ser utilizado como marcador para indicar graus de poluição em ambientes aquáticos. Concluímos que L. laevigatum, espécie ainda não convencionalmente utilizada em bioensaios na área da ecotoxicologia aquática, se apresenta sensível às mudanças em condições ambientais. Palavras-chave: Teste OJIP. Dissipação Energética. Fotossíntese. Macrófita.

Fluorometer

10.17921/1415-6938.2022v26n3p344-352

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Assessment of chromium bioaccumulation in Pseudokirchneriella subcapitata (Korshikov) Hindak by the Central Composite Design (CCD) and Response Surface Methodology (RSM)

Ecotoxicology and Environmental contamination (2013)

Patrícia Carla Giloni-Lima Danieli Delello Marcelo L. M. Cremonez Márcia Noélia Eler Vanderlei Aparecido de Lima

The effects of chromium bioaccumulation in Pseudokirchneriella subcapitata were evaluated by Central Composite Design (CCD), factorial 22 and Response Surface Methodology (RSM). All the models of regression generated by CCD were highly significant, with R2 between 77 and 88%, which is the percentual variability in the response that the model can account for. This is indicative of a satisfactory representation of the process models whose data can be used for simulations of response. The maximum shrinkage biovolume presented 28–69% reduction compared to controls. Results from this study suggest that the smaller algal cells amplify metal binding sites, leading to an increased bioaccumulation and a consequential increased capacity to accumulate chromium. Nevertheless, the absorption capacity decreases for more elevated chromium concentrations and for longer exposure.

Central composite design

10.5132/jbse.2012.02.007

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