Rafaela de Melo Tavares

Universidade Federal de Viçosa

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Luís Augusto Nero

Universidade Federal de Viçosa

Ricardo Seiti Yamatogi

Universidade Federal de Viçosa

Anderson Carlos Camargo

Universidade Federal de Viçosa

Danilo Augusto Lopes da Silva

Universidade Federal de Viçosa

Bruna Torres Furtado Martins

Universidade Federal de Viçosa

Luciano dos Santos Bersot

Universidade Federal do Paraná

Elaine Cristina Pereira De Martinis

Universidade de São Paulo

R. Gomez

Aryele Nunes da Cruz Encide Sampaio

Universidade Estadual Paulista (Unesp)

Náudia da Silva Dias

Universidade de Cuiabá

Cooperative Institutions

Universidade Federal de Viçosa

Universidade Estadual Paulista (Unesp)

Blücher (Germany)

Universidade Federal de Mato Grosso

Universidade de São Paulo

Universidade de Cuiabá

Universidade do Estado de Mato Grosso

Universidade Federal do Rio de Janeiro

Universidade Federal do Pampa

Universidade Federal do Paraná

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Listeria monocytogenes From Farm to Fork in a Brazilian Pork Production Chain

Journal of Food Protection (2020)

Danilo A. L. Silva Clarisse Vieira Botelho Bruna Torres Furtado Martins Rafaela de Melo Tavares Anderson Carlos Camargo

10.4315/0362-028x.jfp-19-379

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Citations (5)

Biology of sponge natural products

Evelyn Richelle-Maurer J. C. Braekman M.J. de Kluyver C. Dévijver Sónia Savluchinske Feio

Sponge

Source

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Citations (0)

Listeria monocytogenes and Pseudomonas spp. Distribution in a Processing Facility of Spotted Sorubim (Pseudoplatystoma corruscans) in Brazil

Journal of Food Protection (2022)

Náudia da Silva Dias Rafaela de Melo Tavares Adelino da Cunha Neto Eduardo Eustáquio de Souza Figueiredo Anderson Carlos Camargo

10.4315/jfp-22-171

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Citations (2)

Interference of sanitizers, NaCl and curing salts on Listeria monocytogenes adhesion and subsequent biofilm formation

Letters in Applied Microbiology (2020)

Diogo Aparecido Lopes Silva Rafaela de Melo Tavares Luís Augusto Nero

Abstract Listeria monocytogenes, a well-known foodborne pathogen and the causative agent of listeriosis, has the ability to persist in food processing environments due to its high adhesion ability in different surfaces, playing an important role in the food industry. The aim of this study was to assess how the main stressing conditions, usually observed in meat processing facilities (sanitizers, NaCl, curing salts), interfere in L. monocytogenes adhesion and biofilm formation. The isolates, representatives of different L. monocytogenes lineages (n = 6) were subjected to four different sanitizers (S1: quaternary ammonium; S2: peracetic acid, hydrogen peroxide and glacial acetic acid, S3: biguanide polyhexamethylene hydrochloride, S4: hydrogen peroxide) to verify adhesion ability and susceptibility based on minimum inhibitory concentration (MIC). In addition, the isolates adhesion and biofilm were assessed up to 72 h under different conditions: sanitizers (MIC values), curing salts and NaCl (both at 5, 7·5, 10%), at different temperatures (4, 12 and 37°C). Despite the effectiveness of sanitizers, isolates presented higher biofilm development when compared to controls in the presence of quaternary ammonium (S1, 1: 1,024) at 4°C, over the tested time (P < 0·05). Furthermore, different responses were observed for the different L. monocytogenes strains tested, providing a better understanding of the persistence of this pathogen in the food processing facilities.

Peracetic acid

Hand sanitizer

10.1111/lam.13374

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Citations (10)

Biofilm growth by Listeria monocytogenes on stainless steel and expression of biofilm-related genes under stressing conditions

World Journal of Microbiology and Biotechnology (2021)

Danilo Augusto Lopes da Silva Rafaela de Melo Tavares Anderson Carlos Camargo Ricardo Seiti Yamatogi Elaine Cristina Pereira De Martinis

10.1007/s11274-021-03092-5

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Citations (11)

Agreement of methods to assess antimicrobial susceptibility using Escherichia coli isolates as target models

Letters in Applied Microbiology (2024)

Milimani Andretta Rafaela de Melo Tavares Andressa Fusieger Ricardo Seiti Yamatogi Luís Augusto Nero

Abstract Antimicrobial susceptibility tests (AST) conducted in vitro offer a range of methods to assess the antimicrobial resistance (AMR) of microorganisms. Escherichia coli, a widely distributed bacterium, is closely linked to the issue of AMR. In this way, the present study aimed to assess the agreement among different in vitro AST methods, including disk diffusion in agar, broth dilution, and agar dilution method. A total of 100 E. coli isolates were analyzed for their resistance levels against six antibiotics: amoxicillin, ceftiofur, ciprofloxacin, chloramphenicol, tetracycline, and sulfamethoxazole + trimethoprim, using the aforementioned AST methods. Standard breakpoint values were employed to classify isolates as resistant, intermediate, or susceptible, and comparisons among the AST methods were conducted by McNemar’s test (P < .05). The obtained data demonstrated equivalence among the AST methods, highlighting the reliability of these standardized classical methodologies. This standardization aids in preventing the inappropriate use of antimicrobials and the dissemination of antimicrobial-resistant microorganisms.

Ceftiofur

Agar dilution

Sulfamethoxazole

10.1093/lambio/ovae009

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Citations (1)

Biofilm Growth By Listeria Monocytogenes On Stainless Steel and Expression of Biofilm-Related Genes Under Stressing Conditions

Research Square (Research Square) (2021)

Danilo Augusto Lopes da Silva Rafaela de Melo Tavares Anderson Carlos Camargo Ricardo Seiti Yamatogi Elaine Cristina Pereira De Martinis

Abstract This research was carried out to assess the ability of L. monocytogenes for adhesion and growth in biofilm on stainless steel coupons under different stressing conditions (NaCl, curing salts and quaternary ammonium compounds - QAC), besides determining the expression of different genes involved in biofilm formation and stress response. Results from crystal violet assay revealed that one isolate carrying a premature stop codon (PMSC) in agrC gene formed high-density biofilms in the presence of QAC or cure salts (7.5% and 10%). Reverse Transcriptase-qPCR results revealed that isolates of L. monocytogenes lineages I and II presented differences in transcriptional profile of genes related to biofilm formation and adaptation to environmental conditions. In conclusion, our results demonstrated how L. monocytogenes can survive, multiply and form biofilm under adverse conditions related to food processing environments. Differences in transcriptional expression were observed, highlighting the role of regulatory gene networks for particular serotypes under different stress responses.

Crystal violet

10.21203/rs.3.rs-408423/v1

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Citations (0)

Interference of the acid stress on the expression of llsX by Listeria monocytogenes pathogenic island 3 (LIPI-3) variants

Food Research International (2020)

Rafaela de Melo Tavares Danilo Augusto Lopes da Silva Anderson Carlos Camargo Ricardo Seiti Yamatogi Luís Augusto Nero

Listeria monocytogenes harbor different virulence factors, with a highly heterogeneous distribution between distinct lineages and serotypes. The Listeria Pathogenicity Island 3 (LIPI-3), mainly described in lineage I, encodes for Listeriolysin S (LLS), a virulence factor expressed by L. monocytogenes in the gastrointestinal tract during in vivo infections. The aim of this study was to carry out a comparative genotypic analysis of LIPI-3 identified in L. monocytogenes isolates obtained in Brazil and subjected to whole genomic sequencing (WGS). In addition, transcription of llsX expression under different acid stress conditions was evaluated by RT-PCR. Homologues of the eight LIPI-3 genes (llsAGHXBYDP) were identified in 15 isolates (all from lineage I) representative of different sequence types: ST1 (n = 3), ST3 (n = 6), ST218 (n = 5) and ST288 (n = 1). Single nucleotide polymorphism (SNP) analysis revealed that genetic variation resulted in modification of the final peptide LLS for ST218 (serogroup IVb-v1) and ST288 (serogroup IIb). Selected strains from ST3 and ST288 were subjected to acid stress conditions and the expression of llsX, a LIPI-3 gene, was observed: only F2365 (4b/ST1) presented llsX expression after six hours of acid stress, indicating relevant differences when compared to isolates IIb (ST3 and 288). The results highlight the presence of genomic variations on LIPI-3 and llsX expression under acid stress conditions, demanding further studies to evaluate if these mutations have an impact on L. monocytogenes virulence in vivo.

Virulence factor

10.1016/j.foodres.2020.109063

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Citations (18)

Relevant foodborne bacteria associated to pork production chain

Advances in food and nutrition research (2024)

Bruna Torres Furtado Martins Anderson Carlos Camargo Rafaela de Melo Tavares Luís Augusto Nero

10.1016/bs.afnr.2024.09.016

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Citations (0)

Characterization of diarrheagenic Escherichia coli from different cattle production systems in Brazil

Food Microbiology (2024)

Rafaela de Melo Tavares Mallú Jagnow Sereno Aryele Nunes da Cruz Encide Sampaio Juliano Gonçalves Pereira Luciano dos Santos Bersot

STX2

10.1016/j.fm.2024.104508

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Citations (1)