Jesús M. de la Fuente

Universidad de Zaragoza

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Co-Authors

Valeria Grazú

Universidad de Zaragoza

100

María Moros

Universidad de Zaragoza

Scott G. Mitchell

Universidad de Zaragoza

Raluca M. Fratila

Instituto de Nanociencia y Materiales de Aragón

Laura De Matteis

Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine

Daxiang Cui

Shanghai Jiao Tong University

Rafael Martín‐Rapún

Universidad de Zaragoza

Lucía Gutiérrez

Instituto de Nanociencia y Materiales de Aragón

Laura Asín

Universidad de Zaragoza

Pablo del Pino

Center for Research in Molecular Medicine and Chronic Diseases

Cooperative Institutions

Consejo Superior de Investigaciones Científicas

181

Universidad de Zaragoza

171

Instituto de Nanociencia y Materiales de Aragón

103

Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine

Shanghai Jiao Tong University

Centre National de la Recherche Scientifique

Instituto de Salud Carlos III

Universidad Autónoma de Madrid

Instituto de Ciencia de Materiales de Sevilla

Universitat de Barcelona

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Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity

Nano Biomedicine and Engineering (2018)

Paula Sanguiñedo Raluca M. Fratila María Belén Estevez Jesús M. de la Fuente Valeria Grazú

Silver nanoparticles have particular properties that contribute to their very promising applications, novel in various fields of science, such as the development of biosensors, the diagnosis and treatment of cancer, the controlled release of drugs and the antimicrobial potential.The biological synthesis of nanoparticles is of great interest over other physical and chemical methods because the use of toxic chemicals and drastic reaction conditions are avoided.The extracellular biosynthesis using fungi could also make downstream processing much easier than the intracellular biosynthesis.One of the main applications of silver nanoparticles is their antimicrobial activity.Several studies have demonstrated the bactericidal properties of silver nanoparticles are different from silver ions, and that they are strongly influenced by their shape, size, concentration and colloidal state.In the present work, the ability of fungal strains from Uruguay to synthesize silver nanoparticles was studied.Eight fungi were able to synthesize nanoparticles.An extensive physicochemical characterization of the nanoparticles was carried out including ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, zeta-potential and gel electrophoretic mobility.According to the characterization and colloidal stability results, nanoparticles from three fungi were selected for antimicrobial activity assays.All nanoparticles were able to inhibit Escherichia coli growth, demonstrating their potential as effective antibacterial agent for use in biomedical applications.

Extracellular polymeric substance

Silver nanoparticle

10.5101/nbe.v10i2.p165-173

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Citations (31)

Field-responsive fluids of carbon-coated magnetic nanoparticles for plant biology and agronomy

Zuny Cifuentes María José Coronado Eduardo Corredor Laura Custardoy Rodrigo Fernández‐Pacheco

Trabajo presentado en el 14th International Conference on Electrorheological Fluids and Magnetorheological Suspensions (ERMR 2014), celebrado en Granada del 7 al 11 de julio de 2014.

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Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake

Nanomaterials (2020)

Rafael Ramírez Jiménez Álvaro Artiga Scott G. Mitchell Rafael Martín‐Rapún Jesús M. de la Fuente

Gold nanoprisms possess remarkable optical properties that make them useful for medical biotechnology applications such as diagnosis and photothermal therapy. However, shape-selective synthesis of gold nanoprisms is not trivial and typically requires either toxic surfactants or time-consuming purification protocols, which can limit their applicability. Here, we show how triangular gold nanoprisms of different sizes can be purified by precipitation using the non-toxic glutathione ligand, thereby removing the need for toxic surfactants and bottleneck purification techniques. The protocol is amenable for direct scaling up as no instrumentation is required in the critical purification step. The new purification method provides a two-fold increased yield in gold nanoprisms compared to electrophoretic filtration, while providing nanoprisms of similar localized surface plasmon resonance wavelength. Crucially, the gold nanoprisms isolated using this methodology show fewer non-specific interactions with cells and lower cellular internalization, which paves the way for a higher selectivity in therapeutic applications.

10.3390/nano10030539

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Citations (11)

A polyoxometalate-assisted approach for synthesis of Pd nanoparticles on graphene nanosheets: synergistic behaviour for enhanced electrocatalytic activity

RSC Advances (2015)

Sara Khadempir Ali Ahmadpour Mohammad Taghi Hamed Mosavian Narges Ashraf Fatemeh F. Bamoharram

A novel activation step has been applied to the Pd/PMo₁₂/GNSs nanohybrid, which enhances the electrocatalytic activity.

Polyoxometalate

10.1039/c5ra01084e

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Citations (36)

Improving immunosensor performance through oriented immobilization of antibodies on carbon nanotube composite surfaces

Biosensors and Bioelectronics (2012)

Sara Puertas María de Gracia Villa Ernest Mendoza Cecilia Jiménez‐Jorquera Jesús M. de la Fuente

Surface Modification

Carbodiimide

Polystyrene

10.1016/j.bios.2012.12.010

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Citations (51)

Adjuvants that improve the ability of carbaryl to remain on the skin of cattle and its residual effect.

Jesús M. de la Fuente J. Rosas

Carbaryl

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Adhesion Forces between LewisX Determinant Antigens as Measured by Atomic Force Microscopy

Angewandte Chemie International Edition (2001)

C. Tromas Javier Rojo Jesús M. de la Fuente África G. Barrientos Ricardo Garcı́a

The adhesion forces between individual molecules of Lewis(X) trisaccharide antigen (Le(X) ) have been measured in water and in calcium solution by using atomic force microscopy (AFM, see graph). These results demonstrate the self-recognition capability of this antigen, and reinforce the hypothesis that carbohydrate-carbohydrate interaction could be considered as the first step in the cell-adhesion process in nature.

Trisaccharide

10.1002/1521-3773(20010817)40:16<3052::aid-anie3052>3.0.co;2-q

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Citations (124)

Bioorthogonal click chemistry meets nanotechnology: covalent immobilization of magnetic nanoparticles on cell membranes

Francisco Javier Idiago López Eduardo Moreno Antolín Raluca M. Fratila Jesús M. de la Fuente

Resumen del trabajo presentado a la IV Reunion de Jovenes Investigadores en Coloides e Interfases, celebrada en Cordoba del 7 al 9 de febrero de 2018.

Bioorthogonal Chemistry

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Polyoxometalate-encapsulated nanoparticles as antimicrobial materials for cultural heritage preservation

Scott G. Mitchell Laura De Matteis Jesús M. de la Fuente

Resumen del trabajo presentado al 5th YOuth in COnservation of CUltural Heritage, celebrado en Madrid (Espana) del 21 al 23 de septiembre de 2016.

Anti-Infective Agents

Polyoxometalate

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Adhesion Forces between LewisX Determinant Antigens as Measured by Atomic Force Microscopy

Angewandte Chemie (2001)

C. Tromas Javier Rojo Jesús M. de la Fuente África G. Barrientos Ricardo Garcı́a

Die Adhäsionskräfte zwischen einzelnen Molekülen des LewisX-Trisaccharids (LeX) wurden in Wasser und in Ca2+-Lösung mit Kraftmikroskopie gemessen (siehe Auftragung). Die Ergebnisse zeigen das Selbsterkennungsvermögen bei diesem Antigen und stützen die Hypothese, dass Kohlenhydrat-Kohlenhydrat-Wechselwirkungen der erste Schritt bei der natürlichen Zelladhäsion sein könnte.

10.1002/1521-3757(20010817)113:16<3142::aid-ange3142>3.0.co;2-k

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Citations (14)