Cristiana Picco

Instituto Biofisika

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Paola Gavazzo

Instituto Biofisika

Joachim Scholz‐Starke

National Research Council

Raffaella Barbieri

National Research Council

Michael Pusch

Czech Academy of Sciences, Institute of Biophysics

ÓM

Óscar Morán

Instituto Biofisika

Alessia Naso

Institute of Genetics and Biophysics

Anna Menini

Scuola Internazionale Superiore di Studi Avanzati

Debora Baroni

National Research Council

Franco Gambale

Texas A&M University

Paolo Zuccolini

Instituto Biofisika

Cooperative Institutions

Instituto Biofisika

National Research Council

University of Genoa

Institute of Genetics and Biophysics

National Academies of Sciences, Engineering, and Medicine

University of Padua

Centre National de la Recherche Scientifique

Italian Institute of Technology

University of Kaiserslautern

Friedrich-Alexander-Universität Erlangen-Nürnberg

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Ferric reduction by a CYBDOM protein counteracts increased iron availability in root meristems induced by phosphorus deficiency

Nature Communications (2024)

Rodolfo A. Maniero Cristiana Picco Anja Hartmann Felipe Engelberger Antonella Gradogna

Abstract To mobilize sparingly available phosphorus (P) in the rhizosphere, many plant species secrete malate to release P sorbed onto (hydr)oxides of aluminum and iron (Fe). In the presence of Fe, malate can provoke Fe over-accumulation in the root apoplast, triggering a series of events that inhibit root growth. Here, we identified HYPERSENSITIVE TO LOW P1 (HYP1), a CYBDOM protein constituted of a DOMON and a cytochrome b 561 domain, as critical to maintain cell elongation and meristem integrity under low P. We demonstrate that HYP1 mediates ascorbate-dependent trans-plasma membrane electron transport and can reduce ferric and cupric substrates in Xenopus laevis oocytes and in planta . HYP1 expression is up-regulated in response to P deficiency in the proximal zone of the root apical meristem. Disruption of HYP1 leads to increased Fe and callose accumulation in the root meristem and causes significant transcriptional changes in roots. We further demonstrate that HYP1 activity overcomes malate-induced Fe accumulation, thereby preventing Fe-dependent root growth arrest in response to low P. Collectively, our results uncover an ascorbate-dependent metalloreductase that is critical to protect root meristems of P-deficient plants from increased Fe availability and provide insights into the physiological function of the yet poorly characterized but ubiquitous CYBDOM proteins.

Root cap

Callose

10.1038/s41467-023-43912-w

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Citations (8)

The VRAC blocker DCPIB directly gates BK channels and increases intracellular Ca2+ in melanoma and pancreatic duct adenocarcinoma cell lines

Biophysical Journal (2022)

Paolo Zuccolini Loretta Ferrera Alessia Remigante Cristiana Picco Raffaella Barbieri

10.1016/j.bpj.2021.11.282

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Citations (2)

Properties of Native and Cloned Cyclic Nucleotide Gated Channels from Bovine

Neurobiology (1996)

Paola Gavazzo Cristiana Picco Letizia Maxia Anna Menini

Transduction (biophysics)

10.1007/978-1-4615-5899-6_6

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Citations (9)

Tonoplast cytochrome b561 is a transmembrane ascorbate‐dependent monodehydroascorbate reductase: functional characterization of electron currents in plant vacuoles

New Phytologist (2023)

Antonella Gradogna Laura Lagostena Sara Beltrami Edoardo Tosato Cristiana Picco

Ascorbate (Asc) is a major redox buffer of plant cells, whose antioxidant activity depends on the ratio with its one-electron oxidation product monodehydroascorbate (MDHA). The cytoplasm contains millimolar concentrations of Asc and soluble enzymes that can regenerate Asc from MDHA or fully oxidized dehydroascorbate. Also, vacuoles contain Asc, but no soluble Asc-regenerating enzymes. Here, we show that vacuoles isolated from Arabidopsis mesophyll cells contain a tonoplast electron transport system that works as a reversible, Asc-dependent transmembrane MDHA oxidoreductase. Electron currents were measured by patch-clamp on isolated vacuoles and found to depend on the availability of Asc (electron donor) and ferricyanide or MDHA (electron acceptors) on opposite sides of the tonoplast. Electron currents were catalyzed by cytochrome b561 isoform A (CYB561A), a tonoplast redox protein with cytoplasmic and luminal Asc binding sites. The Km for Asc of the luminal (4.5 mM) and cytoplasmic site (51 mM) reflected the physiological Asc concentrations in these compartments. The maximal current amplitude was similar in both directions. Mutant plants with impaired CYB561A expression showed no detectable trans-tonoplast electron currents and strong accumulation of leaf anthocyanins under excessive illumination, suggesting a redox-modulation exerted by CYB561A on the typical anthocyanin response to high-light stress.

10.1111/nph.18823

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Citations (8)

Saffron and retinal neurodegenerative diseases: Relevance of chemical composition

Journal of Anatomy (2022)

Maria Anna Maggi Roberto Consonni Laura Ruth Cagliani Gianfranco Prestipino Silvia Bisti

Abstract Saffron is an ancient spice largely used in traditional medicine. It has been found to be effective in treatment of retinal neurodegenerative diseases like age‐related macular degeneration and Stargardt. In the present manuscript, it is shown that saffron’s neuroprotective power is strongly related to the bioactivity of all its chemical components. Nuclear magnetic resonance spectroscopy and “in vitro” experiments confirm the relevance of crocins for saffron efficacy. These results underline the importance of strictly defining the chemical composition of the natural compounds in saffron to optimize their effectiveness in the treatment of diseases.

Relevance

10.1111/joa.13722

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Citations (7)

Time-dependent expression of ryanodine receptors in sea urchin eggs, zygotes and early embryos

Zygote (2021)

G. Percivale C. Angelini Carla Falugi Cristiana Picco Gianfranco Prestipino

In this work, the presence of calcium-dependent calcium channels and their receptors (RyR) has been investigated in Paracentrotus lividus eggs and early embryos, from unfertilized egg to four-blastomere stages. Electrophysiological recordings of RyR single-channel current fluctuations showed that RyRs are functional during the first developmental events with a maximum at zygote stage, c. 40 min after fertilization, corresponding to the first cleavage. The nature of vertebrate-like RyRs active at this stage was established by specific activation/blockade experiments.

Paracentrotus lividus

Blastomere

Cleavage (geology)

10.1017/s0967199421000514

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DKT1, a novel K⁺ channel from carrot, forms functional heteromeric channels with KDC1

FEBS Letters (2004)

Elide Formentin Serena Varotto Alex Costa Patrick Downey Monica Bregante

We report the isolation and characterisation of DKT1, a new carrot K + channel α‐subunit belonging to the Shaker‐like family. DKT1 is expressed in many tissues of the adult plant, suggesting that it may play important roles in both nutrition and other important physiological processes. During embryo development, DKT1 is expressed at later phases implying the involvement of K + in embryo maturation. When co‐expressed with KDC1 in Xenopus oocytes, DKT1 is able to form functional, heteromeric channels, suggesting that possible interactions between these two ion channels in plant tissues may modulate K + uptake.

Shaker

10.1016/j.febslet.2004.07.052

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Citations (23)

Correctors modify the bicarbonate permeability of F508del-CFTR

Scientific Reports (2020)

Michele Fiore Cristiana Picco Óscar Morán

Abstract One of the most common mutations in Cystic Fibrosis (CF) patients is the deletion of the amino acid phenylalanine at position 508. This mutation causes both the protein trafficking defect and an early degradation. Over time, small molecules, called correctors, capable of increasing the amount of mutated channel in the plasma membrane and causing an increase in its transport activity have been developed. This study shows that incubating in vitro cells permanently transfected with the mutated channel with the correctors VX809, VX661 and Corr4a, and the combination of VX809 and Corr4a, a recovery of anion transport activity is observed. Interestingly, the permeability of bicarbonate increases in the cells containing corrected p.F508del CFTR channels is greater than the increase of the halide permeability. These different increases of the permeability of bicarbonate and halides are consistent with the concept that the structural conformation of the pore of the corrector-rescued p.F508del channels would be different than the normal wild type CFTR protein.

Bicarbonate

Transport protein

10.1038/s41598-020-65287-4

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Citations (14)

TMEM16E/ANO5 mutations related to bone dysplasia or muscular dystrophy cause opposite effects on lipid scrambling

Human Mutation (2020)

Eleonora Di Zanni Antonella Gradogna Cristiana Picco Joachim Scholz‐Starke Anna Boccaccio

Mutations in the human TMEM16E/ANO5 gene are causative for gnathodiaphyseal dysplasia (GDD), a rare bone malformation and fragility disorder, and for two types of muscular dystrophy (MD). Previous studies have demonstrated that TMEM16E/ANO5 is a Ca2+ -activated phospholipid scramblase and that the mutation c.1538C>T (p.Thr513Ile) causing GDD leads to a gain-of-function phenotype. Here, using established HEK293-based functional assays, we investigated the effects of MD-related and further GDD-related amino acid exchanges on TMEM16E/ANO5 function in the same expression system. These experiments also revealed that the gradual changes in HEK293 cell morphology observed upon expression of TMEM16E/ANO5GDD mutants are a consequence of aberrant protein activity. Our results collectively demonstrate that, on the level of protein function, MD mutations are associated to loss-of-function and GDD mutations to gain-of-function phenotypes, confirming conjectures made on the basis of inheritance modes.

Proband

10.1002/humu.24006

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Citations (25)

TMEM9B Regulates Endosomal ClC-3 and ClC-4 Transporters

Life (2024)

Margherita Festa Maria Antonietta Coppola Elena Angeli Abraham Tettey‐Matey Alice Giusto

The nine-member CLC gene family of Cl

10.3390/life14081034

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Citations (0)