Development of the eyelid requires coordination of the cellular processes involved in proliferation, cell size alteration, migration, and cell death. C57BL/6J-corneal opacity (B6-Co) mice are mutant mice generated by the administration of N-ethyl-N-nitrosourea (100 mg/kg). They exhibit the eyelids open at birth phenotype, abnormal round cell shape from tightened F-actin bundles in leading edge keratinocytes at E16.5, and gradual corneal opacity with neovessels. The tip of the leading edge in B6-Co mice did not move forward, and demonstrated a sharp peak shape without obvious directionality. Analysis of the biological characteristics of B6-Co mice demonstrated that abnormal migration of keratinocytes could affect eyelid development, but proliferation and apoptosis in B6-Co mice had no effect. Mutant gene mapping and sequence analysis demonstrated that in B6-Co mice, adenosine was inserted into the untranslated regions, between 3030 and 3031, in the mRNA 3'-terminal of Fgf10. In addition, guanine 7112 was substituted by adenine in the Mtap1B mRNA, and an A2333T mutation was identified in Mtap1B. Quantitative real-time polymerase chain reaction analysis showed that expression of the Hbegf gene was significantly down-regulated in the eyelids of B6- Co mice at E16.5, compared to B6 mice. However, the expression of Rock1, Map3k1, and Jnk1 genes did not show any significant changes. Abnormal keratinocyte migration and down-regulated expression of the Hbegf gene might be associated with impaired eyelid development in B6-Co mice.
Abstract Fiber breakage is one of the most negative factors that affect the mechanical performance of unidirectional carbon-fiber (CF)-reinforced composites. In the present study, unidirectional CF-reinforced epoxy (CF/epoxy) laminates with and without fiber breakages located in different layers were manufactured from prepreg via hot compression. The static and cyclic flexural mechanical properties of the unidirectional CF/epoxy laminates were determined through static and low-cycle three-point bending tests. Flexural behavior was observed by using optical microscopy to discuss the failure behavior of the unidirectional CF/epoxy laminates. Results indicated that fiber breakages in different layers led to the static and cyclic mechanical degradation of the unidirectional CF/epoxy laminates. Moreover, fiber breakages in the tension side displayed higher flexural strength and better cyclic performance than those in the compression side.
To fulfill the increasing demand on low-latency content distribution, this paper considers content distribution using generation-based network coding with the belief propagation decoder. We propose a framework to design generation-based network codes via characterizing them as building an irregular graph, and design the code by evaluating the graph. The and-or tree evaluation technique is extended to analyze the decoding performance. By allowing for non-constant generation sizes, we formulate optimization problems based on the analysis to design degree distributions from which generation sizes are drawn. Extensive simulation results show that the design may achieve both low decoding cost and transmission overhead as compared to existing schemes using constant generation sizes, and satisfactory decoding speed can be achieved. The scheme would be of interest to scenarios where (1) the network topology is not known, dynamically changing, and/or has cycles due to cooperation between end users, and (2) computational/memory costs of nodes are of concern but network transmission rate is spare.
Post-microbuckling is a fundamental feature of compressive failure process for the unidirectional-fiber-reinforced composites and laminated composites. The post-microbuckling behavior of composites under compression in the light of the Kevlar49-reinforced 648/BF3.400 (brittle epoxy) and EP (flexible epoxy) is studied, theoretically and experimentally. Analytical results of compressive strength are in good agreement with experimental results, qualitatively and quantitatively. By the experimental research, the post-microbuckling feature of the advancing kink band model is clearly displayed.
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