Enzymes with hydroxylating activity on alkanes have potential application as biotransformation catalysts in chemical and pharmaceutical industry. Genome of Alcanivorax borkumensis, a marine bacterium with hydrocarbon dissimilation activity, contains at least two P450 monooxygenases and two nonheme monooxygenases, AlkB1 and AlkB2, respectively. Presumably, all these enzymes possess alkane hydroxylating activity. Both AlkB1 and AlkB2 are membrane proteins. Two accessory proteins, rubredoxin and rubredoxin reductase, supply the reducing equivalent from nicotinamide adenine dinucleotide phosphate reduced (NADPH to hydroxylases. Rubredoxin reductase catalyses the reduction of rubredoxin by oxidation of NADPH, and rubredoxin transfers the electrons to the alkane hydroxylase to complete the hydroxylation reaction. Here, we sought to investigate the expression of alkB1 gene in Escherichia coli. Therefore, we amplified alkB1 gene from A. borkumensis genome by polymerase chain reaction and cloned it in the expression vector pET26 upstream of His-tag sequence. Predisposed BL21 (DE3) cells were transformed by the recombinant vector. At last, expression of recombinant enzyme was confirmed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blotting. Regarding the potential ability of this enzyme in hydroxylation of long-chained alkanes, the application of it would be studied in petroleum downstream industries.

Rubredoxin

Hydroxylation

10.1177/0748233711416953

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miRNAs and exosomal miRNAs in lung cancer: New emerging players in tumor progression and therapy response

Pathology - Research and Practice (2023)

Mehrdad Hashemi Elaheh Mohandesi Khosroshahi Mehrnaz Kalhor Chegini Maryam Abedi Arash Matinahmadi

Tumor progression

10.1016/j.prp.2023.154906

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STAT3-EMT axis in tumors: Modulation of cancer metastasis, stemness and therapy response

Pharmacological Research (2022)

Mehrdokht Sadrkhanloo Maliheh Entezari Sima Orouei Marzieh Ghollasi Nikoo Fathi

10.1016/j.phrs.2022.106311

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Expression deregulation of SHANK3 gene in children with autism spectrum disorder and attention deficit and hyperactivity disorder

Journal of Human Genetics and Genomics (2021)

Hanieh Bai Seyed Yousef Seyedena Morteza Karimipoor Mehrdad Hashemi

Expression deregulation of SHANK3 gene in children with autism spectrum disorder and attention deficit and hyperactivity disorder

Spectrum disorder

Deregulation

10.52547/jhgg.5.1.70

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MLC901 during sleep deprivation rescues fear memory disruption in rats

Naunyn-Schmiedeberg s Archives of Pharmacology (2019)

Mohammad Nasehi Ameneh Mohammadi Mohaddeseh Ebrahimi-Ghiri Mehrdad Hashemi Mohammad‐Reza Zarrindast

TFAM

Nap

Sleep

Memory Impairment

Privation

10.1007/s00210-018-01612-z

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Curcumin- and Piperine-Loaded Fe 3 O 4 @SiO 2 Magnetic Nanoparticles: Synthesis, Characterization, and Comparison of the Effects on MCF-7 Breast Cancer Cell Line

Research Square (Research Square) (2023)

Jalal Rezaeidian Vahid Naseh Maliheh Entezari Hakimeh Ziyadi Mehrdad Hashemi

Abstract Breast cancer is amongst the commonest cancers which can result in cancer death in women worldwide. The curcumin and piperine are both known as natural polyphenols that have anti-cancer effects. However, quick degradation and metabolism, low bioavailability, and short half-life are some serious issues with the use of curcumin and piperine. Thus, given the importance of magnetic nanoparticles (MNPs) in cancer treatment on the one hand and to overcome the above-mentioned issues on the other, curcumin and piperine were loaded on magnetic iron nanoparticles coated with silica (Fe 3 O 4 @SiO 2 ). Curcumin-loaded Fe 3 O 4 @SiO 2 nanoparticles (Fe 3 O 4 @SiO 2 -curcumine), piperine-loaded Fe 3 O 4 @SiO 2 nanoparticles (Fe 3 O 4 @SiO 2 -piperine), and curcumin/piperine-loaded Fe 3 O 4 @SiO 2 nanoparticles (Fe 3 O 4 @SiO 2 -curcumin/piperine) were prepared by co-participation method followed by drug functionalization. The analysis of prepared magnetic nanoparticles were performed by scanning electron microscopy (SEM), vibrating-sample magnetometer (VSM), Fourier transform infrared spectrometer (FT-IR), and x-ray powder diffraction (XRD). Analysis approved successful loading of drugs on Fe 3 O 4 @SiO 2 core-shell nanoparticles as well as good magnetic properties of curcumin- and piperine-loaded nanoparticles. Toxic effects of novel synthesized MNPs were measured by MTT assay on MCF-7 cancer cell lines. The results of MTT assay showed that all drug-loaded nanoparticles have anticancer effects on MCF-7 breast cancer cells, but impact of Fe 3 O 4 @SiO 2 -curcumin/piperine magnetic nanoparticles are greater than Fe 3 O 4 @SiO 2 -curcumine and Fe 3 O 4 @SiO 2 -piperine with drugs used alone, approving the synergistic effect of these two drugs on MNPs. These results provide a new perspective for cancer treatment using curcumin and piperine together.

Piperine

MCF-7

MTT assay

10.21203/rs.3.rs-2607145/v1

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Collagen 1A1 (COL1A1) and Collagen11A1(COL11A1) as diagnostic biomarkers in Breast, colorectal and gastric cancers

Gene (2023)

Niloufar Salimian Maryam Peymani Kamran Ghaedi Mehrdad Hashemi Ebrahim Rahimi

Diagnostic biomarker

10.1016/j.gene.2023.147867

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Citations (14)

Targeting AMPK signaling in ischemic/reperfusion injury: From molecular mechanism to pharmacological interventions

Cellular Signalling (2022)

Mahshid Deldar Abad Paskeh Ava Asadi Sepideh Mirzaei Mehrdad Hashemi Maliheh Entezari

AMP-Activated Protein Kinase

10.1016/j.cellsig.2022.110323

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Citations (29)

EVALUATION OF CISPLATIN AND CISPLATIN-LOADED MAGNETIC IRON OXIDE NANOPARTICLES ON BCL2 AND BAX GENES IN THE BREAST CANCER T47D CELL LINE

Pathobiology Research (2013)

Mohammad Javad Mokhtari Zhilla Hoseineian Fatemeh Koohpeima Azim Akbarzadeh Mehrdad Hashemi

Breast cancer is the second leading cause of cancer death in women. Cisplatin is a traditional cancer drug commonly used in chemotherapy for killing cancer cells. Modulation at the mRNA levels of apoptotic related genes often correlate with the sensitivity of various types of cancer cells to chemotherapeutic agents. Nanoparticulate drug delivery systems are being developed to effectively deliver smaller doses of chemotherapeutic agents and control drug distribution in the body. In this study, we evaluate the expressions of BCL2 and BAX genes in T47D treated with cisplatin and cisplatin nanoparticles, which can result in a new approach to breast cancer therapy. Methods: In this study, we treated T47D cells with different concentrations of cisplatin and cisplatin nanoparticles at 48 h. The IC50 was determined. We extracted RNA by using RNX solution, after which cDNA was synthesized. The precise primers for the BCL2, BAX and TBP genes were designed by specific software. The quantity of BCL2 and BAX gene expression compared to TBP gene (reference gene) was analyzed using real-time PCR. Results: BCL2 and BAX gene expression levels in T47D cells treated by cisplatin were 0.7 (BCL2) and 1.48 (BAX); in T47D cells treated with cisplatin-loaded nanoparticles, the gene expressions were 0.03 (BCL2) and 2.41 (BAX). Conclusion: In this study, the results have shown that cisplatin-loaded nanoparticles are effective anticancer agents. Cisplatin nanoparticles induce apoptosis in human breast cancer cell lines. We have shown that cisplatin nanoparticles strongly increased cytotoxicity in comparison to the free drug in the T47D cell line.

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